A highly selective probe engineered to detect polarity and distinguish normal cells and tumor cells in tissue sections.

Early diagnostics and therapies for diseases such as cancer are limited by the fact that the inducing factors for the development of cytopathies are not clear. The stable polarity of lipid droplets is a potential biomarker for tumor cells; however, the complex intracellular biological environment poses great difficulties for specific detection of the polarity. Therefore, to meet this pressing challenge, we designed a highly selective fluorescent probe, DCI-Cou-polar, which used the ICT mechanism to differentiate normal cells and tumor cells in tissue sections by detecting changes in the polarities of intracellular lipid droplets. The introduction of a cyclic amine at the 7-position of coumarin (benzoquinolizine coumarin) reduced its ability to donate electrons compared with the diethylamino group, which increased the probe selectivity while retaining the sensitivity to polarity. With NIR emission and large Stokes shifts, DCI-Cou-polar has high sensitivity to polarity, excellent photostability, and biocompatibility, and it tracks lipid droplets with high fidelity. Therefore, we believe that this polarity-sensitive probe provides information on the connection between the polarity of lipid droplets and tumors while improving the development of highly selective polarity probes.

Development of a novel NIR viscosity fluorescent probe for visualizing the kidneys in diabetic mice.

Viscosity is an important parameter for evaluating cell health, and abnormal viscosity can cause a variety of intracellular organelle function disorders. The mitochondria are a key organelle in cells, and the viscosity of the mitochondria determines the state of the cell. In this work, we report a novel near-infrared fluorescent probe, referred to as NI-VD, that has a large Stokes-shift and a satisfactory response multiple. NI-VD can sensitively detect changes in cell viscosity in cells and tissues, and it can effectively avoid interference from the overlap of excitation and emission light. The fluorescence spectrum shows that NI-VD has maximum emission peaks at 730 nm, and the fluorescence intensity is amplified with an increase in the solution viscosity. The response from pure PBS solution to glycerol changes by 13-fold. After confirmation in a variety of cell and biological models, NI-VD can detect the changes in viscosity in mitochondria. Most importantly, this study is the first to visualize the differences between the kidneys of diabetic mice and normal mice. This approach is a new solution for the diagnosis and treatment of diabetic nephropathy.