ABSTRACT
OBJECTIVES: Autologous tenocyte implantation (OrthoATI™) therapy has demonstrated efficacy in treating patients with tendinopathy at various anatomical sites. This study evaluates the effect of patient age, gender, and tendon biopsy site on morphology, growth, and gene expression of autologous tendon cells used to treat chronic tendinopathy. METHODS: Patients undergoing OrthoATI™ for tendinopathies between 2020 and 2022 were initially treated by biopsies taken from patella tendon (PT) or palmaris longus tendon (PL). The biopsies were sent to a Good Manufacturing Practice (GMP) cell laboratory where tendon cells were isolated, cultured, and expanded for four to six weeks. Cell morphology was assessed using phase contrast microscopy. Droplet digital PCR (ddPCR) was utilized for gene expression analysis. Dichotomous results were compared between groups using x2 or Fisher's exact tests with no adjustment for multiple comparisons. The nonparametric Mann-Whitney U and Kruskal-Wallis tests were utilized for the sex and age (<35y, 35-44y, 45-54y, >55y) analyses, respectively. All analyses were performed using IBM SPSS v27, and a two-tailed P-value of <0.05 was considered statistically significant. RESULTS: 149 patients were included in the analysis. The PT was biopsied in 63 patients, and PL in 86 patients. There were no observer effects for age and gender between the PT and PL groups. There was no statistical significance between the PT and PL tendons for cell morphology, average cell population doubling time (PDT) (PT 83.9 vs PL 82.7 âh, p â= â0.482), cellular yield (PT 16.2 vs PL 15.2 â× â106, p â= â0.099), and cell viability (PT 98.7 vs PL 99.0%, p â= â0.277). Additionally, ddPCR analyses showed no statistical significance found in tenogenic gene expression, including collagen type I (COL1, p â= â0.86), tenomodulin (TNMD, p â= â0.837) and scleraxis (SCX, p â= â0.331) between PT- and PL-derived tendon cells. An age stratification analysis found no effect on growth and gene expression. COL1 was found to be higher in males when compared to females (P â< â0.001), but otherwise no difference was seen in growth and gene expression in the gender analysis. No postbiopsy clinical complications were reported for either group. CONCLUSION: This study has shown that the growth and bioactivities of tendon cells from tendon biopsies for OrthoATI™ are not affected by tendon donor site and age. LEVEL OF EVIDENCE: IV.
Subject(s)
Tendinopathy , Tenocytes , Transplantation, Autologous , Humans , Tendinopathy/therapy , Female , Male , Middle Aged , Adult , Tenocytes/metabolism , Transplantation, Autologous/methods , Age Factors , Chronic Disease , Tendons , Transplant Donor Site , Biopsy/methods , Sex FactorsABSTRACT
BACKGROUND: It is controversial whether or not the carpometacarpal joint (CMCJ) should be included in total wrist arthrodesis (TWA). Complications commonly occur at this site and studies examining its inclusion and exclusion are conflicting. A randomised clinical trial comparing wrist arthrodesis with CMCJ arthrodesis and spanning plate to wrist arthrodesis with CMCJ preservation and non-CMCJ spanning plate has not been performed. METHOD: A single centre randomised clinical trial including 120 adults with end-stage isolated wrist arthritis will be performed to compare TWA with and without the CMCJ included in the arthrodesis. The primary outcome is complications in the first post-operative year. Secondary outcomes are Disabilities of the Arm, Shoulder and Hand (DASH) score, Patient Rated Wrist Evaluation (PRWE) and grip strength measured at 1, 2 and 5 years. Late complications, return to work and satisfaction will also be recorded. DISCUSSION: It is unknown whether the CMCJ should be included in TWA. This trial will contribute to an improved understanding of optimal management of the CMCJ in total wrist arthrodesis. TRIAL REGISTRATION: This trial was prospectively registered with the Australia New Zealand Clinical Trials Registry with identifying number ACTRN12621000169842 on the 16th February 2021. WHO: U1111-12626523. ANZCTR: ACTRN12621000169842.
Subject(s)
Carpometacarpal Joints , Arthrodesis/adverse effects , Bone Plates , Carpometacarpal Joints/diagnostic imaging , Carpometacarpal Joints/surgery , Hand Strength , Humans , Randomized Controlled Trials as Topic , Treatment Outcome , Wrist , Wrist Joint/diagnostic imaging , Wrist Joint/surgeryABSTRACT
BACKGROUND: Medical imaging has an increasing role in the early diagnosis and management of sports injuries. Post-injury management decisions are becoming more heavily dependent on imaging findings. Timely use of appropriate imaging for sports injuries is required for optimal outcomes. OBJECTIVE: The aims of this article are to discuss a range of imaging options that are available in the clinical setting of sports injury, and to provide an insight into the role of each imaging modality and image-guided procedures in enabling early return to activity for the athlete. DISCUSSION: Although imaging is playing an increasing part in the diagnosis and management of sports injuries, the role of each modality is not usually clear because of a considerable overlap of roles of these modalities. A succinct summary of imaging modalities commonly used in the clinical setting of sporting injuries is presented in this article, including the relative merits for choosing the correct imaging modality.
Subject(s)
Athletic Injuries/diagnostic imaging , Diagnostic Imaging/trends , Athletic Injuries/diagnosis , Diagnostic Imaging/methods , Humans , Magnetic Resonance Imaging/methods , Magnetic Resonance Imaging/trends , Radiography/methods , Radiography/trends , Tomography, X-Ray Computed/methods , Tomography, X-Ray Computed/trends , Ultrasonography/methods , Ultrasonography/trendsABSTRACT
Calcific tendinosis is characterized by macroscopic deposits of hydroxyapatite within the tendon. Commonest location is rotator cuff of shoulder. Cortical erosion with intraosseous migration of calcium deposits is rare complication of this condition. It may be confused with neoplasm or infection, resulting in unnecessary biopsies or interventions. The knowledge of this uncommon complication and its appearance should enable its detection by imaging and lead to specific treatment.
Subject(s)
Calcinosis/complications , Calcinosis/diagnostic imaging , Rotator Cuff/diagnostic imaging , Tendinopathy/complications , Tendinopathy/diagnostic imaging , Adrenal Cortex Hormones/administration & dosage , Adult , Calcinosis/therapy , Female , Humans , Magnetic Resonance Imaging/methods , Rotator Cuff/drug effects , Rotator Cuff/surgery , Tendinopathy/therapy , Tomography, X-Ray Computed/methods , Ultrasonography, Interventional/methodsABSTRACT
BACKGROUND: Proximal humeral fractures are common in older patients. The majority are minimally displaced and are associated with good outcomes after nonoperative treatment. Poorer outcomes are associated with displaced, multipart fractures. There is no clear benefit from surgical fracture fixation compared to nonoperative treatment. Replacement of the fractured humeral head with a hemiarthroplasty is another treatment option, but has not been shown to be clearly superior to nonoperative treatment or internal fixation. Recently, reverse total shoulder arthroplasty has been used to treat these fractures, particularly in the older population with several case series demonstrating good outcomes. No comparative trial has been performed to test the effectiveness of reverse total shoulder arthroplasty against nonoperative treatment. METHODS/DESIGN: ReShAPE (Reverse Shoulder Arthroplasty for the treatment of Proximal humeral fractures in the Elderly) is a multicenter combined randomized and observational study. The primary objective is to compare pain and function 12 months post fracture using the American Shoulder and Elbow Society (ASES) score in patients aged 70 years or older with three- and four-part proximal humeral fractures treated by either reverse shoulder arthroplasty or nonoperative treatment. Secondary outcome measures will include the DASH (Disability of the Arm, Shoulder and Hand) score, the EQ-5D (EuroQol Health Survey), the EQ-VAS, pain, radiological parameters and complications. DISCUSSION: The study will assess the effectiveness of reverse shoulder arthroplasty for complex proximal humeral fractures and thereby guide treatment of a common injury in the older population. TRIAL REGISTRATION: World Health Organization Universal Trial Number (WHO UTN): U1111-1180-5452 . Registered on 10 March 2016. Australian and New Zealand Clinical Trials Registry (ANZCTR): 12616000345482 . Registered on 16 March 2016.
Subject(s)
Arthroplasty, Replacement, Shoulder/methods , Humerus/surgery , Shoulder Fractures/surgery , Age Factors , Aged , Arthroplasty, Replacement, Shoulder/adverse effects , Australia , Clinical Protocols , Disability Evaluation , Female , Humans , Humerus/diagnostic imaging , Humerus/physiopathology , Male , Pain Measurement , Recovery of Function , Research Design , Shoulder Fractures/diagnosis , Shoulder Fractures/physiopathology , Shoulder Pain/diagnosis , Shoulder Pain/etiology , Shoulder Pain/prevention & control , Surveys and Questionnaires , Time Factors , Treatment OutcomeABSTRACT
Chronic elbow subluxation is uncommon but challenging to treat. Although many treatment methods have been described, few clinical outcome studies have been done because of the infrequency of the condition. Reconstruction has been better defined for radial head deficiencies than for the coronoid. Recognizing and repairing or reconstructing any associated soft-tissue deficiencies are important to a good outcome. For some patients, protecting the reconstruction with a hinged external fixator is recommended to allow early range of motion.