ABSTRACT
Lentinula edodes polysaccharides from at four different development stages (referred to L1, L2, L3 and L4, respectively) were extracted by hot water method, and graded ethanol precipitation to final concentration of 20%, 50% and 70%, then12 crude polysaccharide fractions (referred to L1P20, L2P20, L3P20; L4P20, L1P50, L2P50, L3P50, L4P50 and L1P70, L2P70, L3P70, L4P70, respectively) were obtained. Physicochemical properties and exoteric bioactivities of the crude polysaccharide fractions were measured. The results of physicochemical properties revealed that extraction yields of P20 fractions were significantly higher than those of P50 and P70 fractions, and the contents of polysaccharide and ß-glucan in L3P50 fractions were higher, and the viscosity-average molecular weight reached a maximum at L2, and high molecular weight polysaccharides could be obtained at a low alcohol concentration in P20 fractions, and the glycosidic bonds were found to exist in all crude polysaccharide fractions. These crude polysaccharide fractions showed different bioactivities, wherein the polysaccharides of higher molecular weight in P20 fractions had greater bioactivity. These results showed that immature stage of Lentinula edodes was the optimal harvest time for obtaining higher bioactivity of crude polysaccharides.
Subject(s)
Biological Products/chemistry , Biological Products/pharmacology , Chemical Phenomena , Fungal Polysaccharides/chemistry , Fungal Polysaccharides/pharmacology , Shiitake Mushrooms/chemistry , Animals , Antioxidants/chemistry , Antioxidants/pharmacology , Fungal Proteins , Mice , Molecular Weight , Nitric Oxide , RAW 264.7 Cells , Shiitake Mushrooms/growth & development , Spectrum Analysis , ViscosityABSTRACT
This paper explored the effects of Tween 80 on the biomass, intracellular polysaccharide (IPS) content, fermentation parameters, the pellets size of mycelium, and the antioxidant activity of IPS in Lentinus edodes liquid fermentation. With adding to Tween 80, the outputs of biomass and IPS increased during the L. edodes fermentation, respectively, while the reducing sugar content was decreased, as well as, the time courses of pH value were different. It was also shown that the addition of Tween 80 could protect the intact of pellets from breaking down. The effects of Tween 80 on the main structure of IPS were no obvious, and the IPS were revealed similar infrared spectrum, as was indicated by the infrared spectrum analysis. Improvements in the scavenging capacity of DPPH radicals of IPS were observed in Tween 80 treated group compared with the control group. Tween 80 exerts impacts on the liquid fermentation of L. edodes.