ABSTRACT
OBJECTIVE: To extract the essential oils from the Seedlings, the Aseptic Seedlings and the Tissue Culture Seedlings of Thymus vulgaris and analyze their chemical components and the relative contents. METHODS: The essential oils were extracted by steam distillation, the chemical components and the relative contents were identified and analyzed by gas chromatography-mass spectrometry (GC/MS) and peak area normalization method. RESULTS: The main chemical components of essential oil in these three samples had no significant difference, they all contained the main components of essential oil in Thymus vulgaris: Thymol, Carvacrol, o-Cymene, gamma-Terpinene, Caryophyllene et al. and only had a slight difference in the relative content. CONCLUSION: This study provides important theoretical foundation and data reference for further study on production of essential oil in thyme by tissue culture technology.
Subject(s)
Oils, Volatile/chemistry , Sesquiterpenes/analysis , Thymol/analysis , Thymus Plant/chemistry , Cyclohexane Monoterpenes , Cymenes , Gas Chromatography-Mass Spectrometry/methods , Molecular Structure , Monoterpenes/analysis , Monoterpenes/chemistry , Oils, Volatile/isolation & purification , Sesquiterpenes/chemistry , Thymol/chemistry , Tissue Culture TechniquesABSTRACT
OBJECTIVE: To prepare an oral nutritional liquid for protecting liver and antialcoholic from defatted oyster powder. METHODS: The orthogonal design was used to determine the optimal enzymatic hydrolytic condition and the enzymolysis liquid from oyster was deodorized and seasoned. RESULTS: The optimum hydrolytic conditions were determined as follows: defatted oyster powder added concentration of 2%, ratio of enzyme to base material 10%, enzymatic hydrolytic time 90 min, temperature 50 degrees C, pH 6.2. The content of glycogen, crude protein and amino acids in the oral nutritional liquid were 461 mg/100 mL, 381 mg/100 mL and 294.5 mg/100 mL, respectively. CONCLUSIONS: The processing conditions are stable and reliable and can be used to provide reference for further pilot scale production.
Subject(s)
Alcoholism/prevention & control , Materia Medica/chemistry , Materia Medica/isolation & purification , Ostreidae , Papain/metabolism , Administration, Oral , Amino Acids/analysis , Animals , Glycogen/analysis , Hydrolysis , Powders , Technology, Pharmaceutical/methods , TemperatureABSTRACT
OBJECTIVE: To purify the oleuropein crude extracts by sephadex LH-20 column chromatograph. METHODS: This experiment used fast protein chromatography system (AKTA FPLC) produced by Amersham, Sweden. The chromatography column (20 mm x 300 mm) was matched with protein purification instrument. Sephadex LH-20 was used in the Fast Protein Liquid Chromatography columns. The mobile phase was 50% ethanol with a flow velocity of 1.0 mL per minute and the detection wavelength was 254 nm. The content of oleuropein was determined by HPLC. RESULTS: The purity of oleuropein was 82.9% after passing the column twice when the sample volume was 2 mL. CONCLUSION: Sephadex LH-20 can be re-used and the regeneration is convenient, it also provides a reference for the production of oleuropein.
Subject(s)
Chromatography, Gel/methods , Chromatography, High Pressure Liquid/methods , Olea/chemistry , Pyrans/isolation & purification , Technology, Pharmaceutical/methods , Dextrans , Ethanol/chemistry , Iridoid Glucosides , Iridoids , Pyrans/analysis , Pyrans/chemistry , Reproducibility of ResultsABSTRACT
OBJECTIVE: To study the conditions and parameters of the enrichment of oleuropein with macroporous resin. METHODS: Aqueous extract of Olea europaea leaves prepared through microwave extraction was adsorbed directly with macroporous resin D-101 and the impurities such sugar were washed out by water then oleuropein was eluted by 70% ethanol. HPLC was used to determine the content of oleuropein. RESULTS: The contents of oleuropein increased from 5% to 21.6% in the solid, with 88.6% of recovery rate. CONCLUSION: Macroporous resin D-101 fits in purification of water-soluble oleuropein. The process is simple and convenient and can be used for industrial production.