ABSTRACT
The construction of a novel enzyme-controlled nanomachine with multiple release mechanisms for on-command delivery is described. This nanodevice was assembled by modifying mesoporous silica nanoparticles with 2-(benzo[d]thiazol-2-yl)phenyl 4-aminobenzoate moieties, and further capped with ß-cyclodextrin-modified glucose oxidase neoglycoenzyme. The device released the encapsulated payload in the presence of H2O2 and acidic media. The use of glucose as an input chemical signal also triggered cargo release through the enzymatic production of gluconic acid and hydrogen peroxide, and the subsequent disruption of the gating mechanism at the mesoporous surface. The nanodevice was successfully employed for the enzyme-controlled release of doxorubicin in HeLa cancer cells.
Subject(s)
Glucose Oxidase , beta-Cyclodextrins , Delayed-Action Preparations , Doxorubicin/pharmacology , Glucose , Humans , Hydrogen Peroxide , Porosity , Silicon Dioxide , para-AminobenzoatesABSTRACT
Antibiotics are found in natural waters, raising concern about their human and environmental toxicity and the wide occurrence of antibiotic resistant bacteria. The antibiotic resistance crisis is attributed to the overuse and misuse of these medications. Particularly, sulfamethazine (SMT), an antibiotic commonly used in pigs and cattle for the treatment of bacterial diseases, has been detected in the natural environment (soil and water). Among all the technologies developed to combat the deteriorating water quality and control antimicrobial resistance, heterogeneous photocatalysis should be highlighted for the degradation of refractory organic compounds. Here, we described the SMT adsorption and photodegradation capacity of a highly porous and robust zirconium-based MOF UiO-66 under realistic conditions, and its potential recyclability. Further, its SMT removal capacity was improved by functionalizing the MOF porosity (28.5% of SMT adsorption in 24 h for nanoUiO-66-NH2), and nanosizing the MOF (100% SMT photodegradation in only 4 h for nanoUiO-66). Finally, the safety of the formed by-product during SMT photodegradation was confirmed, reinforcing the potential of the application of UiO-66 in water remediation.
