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1.
Blood Cells Mol Dis ; 36(2): 259-64, 2006.
Article in English | MEDLINE | ID: mdl-16458028

ABSTRACT

The human ribosomal protein S19 gene (RPS19) is mutated in approximately 20% of patients with Diamond-Blackfan anemia (DBA), a congenital disease with a specific defect in erythropoiesis. The clinical expression of DBA is highly variable, and subclinical phenotypes may be revealed by elevated erythrocyte deaminase (eADA) activity only. In mice, complete loss of Rps19 results in early embryonic lethality whereas Rps19+/- mice are viable and without major abnormalities including the hematopoietic system. We have performed a detailed analysis of the Rps19+/- mice. We estimated the Rps19 levels in hematopoietic tissues and we analyzed erythrocyte deaminase activity and globin isoforms which are used as markers for DBA. The effect of a disrupted Rps19 allele on a different genetic background was investigated as well as the response to erythropoietin (EPO). From our results, we argue that the loss of one Rps19 allele in mice is fully compensated for at the transcriptional level with preservation of erythropoiesis.


Subject(s)
Anemia, Diamond-Blackfan/genetics , Erythropoiesis/genetics , Ribosomal Proteins/genetics , Animals , Biomarkers/analysis , Erythropoietin/pharmacology , Heterozygote , Mice , Mice, Knockout , Ribosomal Proteins/deficiency , Transcription, Genetic
2.
Int Immunol ; 12(7): 995-1003, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10882411

ABSTRACT

During lymphocyte activation, changes in cell morphology are commonly observed. This reflects cell functions important for the regulation of immune responses such as cell adhesion or cell migration. Notably, IL-4 has been shown to induce adhesion and locomotion in B cells, and we have recently described that IL-4 causes dramatic changes in B cell morphology. Thus, such B cells spread with dendritic cell protrusions and produce microvilli-like structures. The molecular mechanisms by which IL-4 induces these complex changes are currently unknown. Two signal transduction pathways are well described for IL-4, i.e. one involving insulin receptor substrate (IRS)-2 and a Janus kinase (JAK)/ signal transducer and activator of transcription (STAT) pathway mediated by STAT6. In this study we therefore used B cells from STAT6-deficient mice to address the question of a possible STAT6 dependence in IL-4-induced morphology changes. By light and electron microscopy, cell spreading and polarization were found to be severely impaired and microvilli formation was reduced. In contrast, only mild impairment was observed in cell adhesion in B cells from STAT6-deficient mice. Our results show that adhesion can be induced in the absence of STAT6. However, expression of STAT6 is necessary for optimal responses in both cell adhesion and microvilli induction. STAT6 is also essential to allow an IL-4-dependent spreading or polarization response. A possible interpretation of our results is that STAT6-dependent expression of a specific gene or genes is required for IL-4 to affect changes in B cell morphology.


Subject(s)
B-Lymphocytes/drug effects , Cytoskeleton/drug effects , Interleukin-4/pharmacology , Trans-Activators/physiology , Animals , B-Lymphocytes/pathology , B-Lymphocytes/physiology , Cells, Cultured , DNA/biosynthesis , Insulin Receptor Substrate Proteins , Intracellular Signaling Peptides and Proteins , Lipopolysaccharides/pharmacology , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Phosphoproteins/physiology , Receptors, Interleukin-4/analysis , STAT6 Transcription Factor
3.
J Immunol ; 160(11): 5366-73, 1998 Jun 01.
Article in English | MEDLINE | ID: mdl-9605136

ABSTRACT

Lymphocyte activation is often accompanied by changes in cell morphology, for example, in cell adhesion or motility. IL-4 is a cytokine exerting many effects on B lymphocytes. In this study, we show that stimulation with LPS in combination with IL-4, but not LPS or IL-4 alone, results in a pronounced dendritic morphology of B cells. Using a culture system in which Abs directed to B cell surface markers are immobilized on the tissue culture plastic, we find that cell spreading can be mediated by a variety of Abs, including anti-CD44, -CD23, -LFA-1, -VLA-4, -ICAM-1, and -Ig. B cells stimulated with anti-Ig Abs plus IL-4, or anti-CD40 Abs in the presence or absence of IL-4, are also induced to spread, while IL-2, IL-5, or IL-10 in combination with LPS or alone fail to induce this. Spreading correlates with induction of tight cell aggregation. It is sensitive to cytochalasin B, indicating a requirement for intact actin cytoskeleton. CD44 is selectively detected in the detergent-insoluble fraction of cell lysates prepared from LPS plus IL-4-stimulated B cell cultures after Ab cross-linking of CD44, suggesting a membrane protein-cytoskeleton interaction. Interestingly, electron microscopy studies reveal induction of microvilli-like structures on LPS plus IL-4-stimulated blasts, suggesting that IL-4 can influence cell morphology on an ultra-structural level. In summary, our data show that stimulation with LPS plus IL-4 or ligation of CD40 is capable of inducing dramatic morphologic changes in murine B cells, which correlates with in vitro induction of strong cell adhesion.


Subject(s)
B-Lymphocytes/cytology , Cytoskeleton/drug effects , Interleukin-4/pharmacology , Animals , Antibodies, Anti-Idiotypic/pharmacology , Antibodies, Monoclonal/pharmacology , B-Lymphocytes/immunology , B-Lymphocytes/ultrastructure , CD40 Antigens/immunology , Cell Movement/drug effects , Cell Movement/immunology , Cytoskeleton/immunology , Drug Synergism , Hyaluronan Receptors/metabolism , Immune Sera/pharmacology , Immunoglobulin M/immunology , Interleukin-10/pharmacology , Interleukin-2/pharmacology , Interleukin-5/pharmacology , Kinetics , Lipopolysaccharides/pharmacology , Lymphocyte Activation/drug effects , Lymphocyte Activation/immunology , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Mice, Knockout , Microvilli/ultrastructure
4.
Eur J Immunol ; 25(5): 1224-9, 1995 May.
Article in English | MEDLINE | ID: mdl-7774626

ABSTRACT

CD23 is a low-affinity receptor for IgE (Fc epsilon RII). Functions attributed to CD23 not involving IgE suggest that it interacts with ligands other than IgE. CD21 has recently been described as a counter ligand for CD23. A number of lines of evidence have implicated CD23 as an adhesion molecule in human B cells. We have investigated the role of CD23 in homotypic B cell aggregation in the mouse, using lipopolysaccharide plus interleukin-4-induced aggregation as a model system. In this system high levels of aggregation are accompanied by a massive up-regulation of CD23 expression. However, in contrast to what has been observed in human B cells, we find no evidence of a role for CD23 in homotypic adhesion of murine B cells.


Subject(s)
B-Lymphocyte Subsets/immunology , Cell Adhesion Molecules/physiology , Receptors, IgE/physiology , Animals , CHO Cells , Cell Aggregation , Cricetinae , Female , Goats , Immunoglobulin Fab Fragments/immunology , Interleukin-4/pharmacology , Lipopolysaccharides/pharmacology , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Mice, Knockout , Rabbits , Receptors, IgE/deficiency , Receptors, IgE/genetics , Recombinant Proteins/pharmacology , Transfection
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