ABSTRACT
The aim of this study was to evaluate the fermentation of dietary fiber from green bean (Phaseolus vulgaris) and prickly pear shell (Opuntia ficus-indica) by Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum 450B growing as mono-culture and co-culture, the fermentation products, and proteins expressed during this process. The analysis of the fermentation profile showed a major growth of bacteria in the culture media of each dietary fiber supplemented with glucose, and particularly B. bifidum 450B at 48 h showed the highest growth. In the case of the co-culture, the growth was lower indicating the possible negative interaction between L. acidophilus LA-5 and B. bifidum 450B and may be due to the less amount of carbohydrates and the high content of non-soluble fiber that affected the nutrients availability for the bacterial strains. The pH changes indicated the presence of short-chain fatty acids (SCFAs), being acetate (46-100%) the main SCFA. Changes in the proteome concerned proteins that are involved in carbohydrate and other carbohydrate pathways. The characterization of the bacteria according to the growth, metabolites, and proteins expressed allows understanding the response to the change of environmental conditions and could be useful to understand L. acidophilus LA-5 and B. bifidum 450B strains' adaptation to specific applications.
Subject(s)
Bifidobacterium bifidum/metabolism , Dietary Fiber/metabolism , Lactobacillus acidophilus/metabolism , Opuntia/metabolism , Phaseolus/metabolism , Environmental Microbiology , Fatty Acids, Volatile/metabolism , Fermentation/physiologyABSTRACT
This paper aims to present the usefulness of the different diagnosis imaging methods (anatomical and functional) in the characterization of bone injury. Any data, however insignificant, is justified and should be specified. In this case, the discrepancy between the vascular and pool phases in the bone scintigraphy with 99mTc-MDP reveals revealed a lesion with an intense reaction secondary to the "foreign body effect", which is not necessarily malignant.
Subject(s)
Femoral Neoplasms/diagnostic imaging , Osteoma, Osteoid/diagnostic imaging , Radiopharmaceuticals , Technetium Tc 99m Medronate/analogs & derivatives , Adolescent , Diagnosis, Differential , Foreign-Body Reaction/diagnostic imaging , Humans , Magnetic Resonance Imaging , Radionuclide ImagingABSTRACT
El objetivo es presentar la utilidad de los distintos métodos de diagnóstico por imagen (anatómicos y funcionales), en la caracterización de una lesión ósea.Cualquier dato, por insignificante que este sea, tiene su justificación y debe ser especificado. En este caso, la discrepancia en la gammagrafía ósea con 99mTc-MDP, entre las fases vascular y 'pool', revela a una lesión con intensa reacción secundaria al efecto de 'cuerpo extraño', que necesariamente no tiene que ser maligna (AU)
Subject(s)
Adolescent , Humans , Technetium Tc 99m Medronate , Radiopharmaceuticals , Osteoma, Osteoid , Diagnosis, Differential , Magnetic Resonance Imaging , Foreign-Body Reaction , Femoral NeoplasmsABSTRACT
The tomographic red blood cell imaging is useful in the investigation the cavernous hemangioma of the liver (specificity: 100%). In all positive studies, with negative cytology for malignancy, and absence of complications (bleeding, growth, etc.) It'll could adopt conservation aptness.
