ABSTRACT
Spectrofluorometric method for determination of promethazine hydrochloride, thioridazine hydrochloride and perazine dimaleate in the pure form and in drugs is described. Fluorescence excitation spectra of series of aqueous solutions were measured. The fluorescence signal was found to be a linear function of the promethazine hydrochloride (PTM) concentration in the range: 0.30-20.02 ppm, thioridazine hydrochloride (TR): (0.43-21.70 ppm and perazine dimaleate (PDM): 0.85-50.60 ppm. The excitation spectra were used for determination of phenothiazine derivatives in pharmaceutical formulations such as: Diphergan, Thioridazin and Pernazinum. The influence of K+, Na+, Mg2+ i Ca2+ cations on the fluorescence intensity of phenothiazine derivatives was also studied.
Subject(s)
Phenothiazines/analysis , Antipsychotic Agents/analysis , Spectrometry, Fluorescence , TabletsABSTRACT
Five tricyclic antidepressants: imipramine hydrochloride, noxiptilin hydrochloride, hydroxyzine dihydrochloride, diethiazine hydrochloride and amitryptyline hydrochloride were determined by the capillary electrophoresis method. The determinations were performed by using a fused sillica capillary (60 cm x 50 microns i.d., effective length 53 cm). Acetate buffer (25 mM ammonium acetate and 1 mM acetic acid) in (1:1) methanol:water solution was used as an electrolyte. The determination voltage was 20 kV. The UV-detector at 214 nm was applied.
