An origami microfluidic paper device based on core-shell Cu@Cu2S@N-doped carbon hollow nanocubes.

BACKGROUD: The global prevalence of diabetes mellitus, a serious chronic disease with fatal consequences for millions annually, is of utmost concern. The development of efficient and simple devices for monitoring glucose levels is of utmost significance in managing diabetes. The advancement of nanotechnology has resulted in the indispensable utilization of advanced nanomaterials in high-performance glucose sensors. Modulating the morphology and intricate composition of transition metals represents a viable approach to exploit their structure/function correlation, thereby achieving optimal electrocatalytic performance of the synthesized catalysts. RESULTS: Herein, a sensitive and rapid Cu-encapsulated Cu2S@nitrogen-doped carbon (Cu@Cu2S@N-C) hollow nanocubes-functionalized microfluidic paper-based analytical device (µ-PAD) was fabricated. Through a delicate sacrificial template/interface technique and thermal decomposition, inter-connected hollow networks were formed to boost the active sites, and the carbon shell was coated to protect Cu from being oxidation. For application, the constructed µ-PAD is used for glucose sensing utilizing an origami automated sample pretreatment system enabled by a simple application of strong alkaline solution on wax paper. Under optimal circumstances, the Cu@Cu2S@N-C electrochemical biosensor exhibits broad detection range of 2-7500 µM (R2 = 0.996) with low detection limit of 0.16 µM (S/N = 3) and high sensitivity of 1996 µA mM-1 cm-2. Additionally, the constructed µ-PAD also exhibited excellent selectivity, stability, and reproducibility. SIGNIFICANCE: By rationally designing the double-shell hollow nanostructure and introducing Cu-encapsulated inner layer, the synthesized Cu@Cu2S@N-C hollow nanocubes show large specific surface area, short diffusion channels, and high stability. The proposed origami µ-PAD has been successfully applied to serum samples without any additional sample preparation steps for glucose determination, offering a new perspective for early nonenzymatic glucose diagnosis.

The microbial composition of pancreatic ductal adenocarcinoma: A systematic review of 16S rRNA gene sequencing.

BACKGROUND: Pancreatic cancer, specifically pancreatic ductal adenocarcinoma (PDAC), continues to pose a significant clinical and scientific challenge. The most significant finding of recent years is that PDAC tumours harbour their specific microbiome, which differs amongst tumour entities and is distinct from healthy tissue. This review aims to evaluate and summarise all PDAC studies that have used the next-generation technique, 16S rRNA gene amplicon sequencing within each bodily compartment. As well as establishing a causal relationship between PDAC and the microbiome. MATERIALS AND METHODS: This systematic review was carried out according to the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines. A comprehensive search strategy was designed, and 1727 studies were analysed. RESULTS: In total, 38 studies were selected for qualitative analysis and summarised significant PDAC bacterial signatures. Despite the growing amount of data provided, we are not able to state a universal 16S rRNA gene microbial signature that can be used for PDAC screening. This is most certainly due to the heterogeneity of the presentation of results, lack of available datasets and the intrinsic selection bias between studies. CONCLUSION: Several key studies have begun to shed light on causality and the influence the microbiome constituents and their produced metabolites could play in tumorigenesis and influencing outcomes. The challenge in this field is to shape the available microbial data into targetable signatures. Making sequenced data readily available is critical, coupled with the coordinated standardisation of data and the need for consensus guidelines in studies investigating the microbiome in PDAC.

Partially coherent Pearcey-Gauss source with hyperbolic sine correlation of the spatial spectrum.

By designing the intricate coherence structure, we are able to create a desired beam profile and trajectory. Our research focus lies on the Fourier plane, specifically emphasizing the coherence of spatial frequencies, and we find it can be seen as a constant system response. A theoretical framework is developed, and experimental studies are conducted to generate a light field of the spatial spectrum with a complex correlation using the pseudo-mode superposition method. We successfully produce partially coherent Pearcey-Gauss beams whose spatial spectrum is hyperbolic sine correlational. Interestingly, these beams maintain the distinctive propagation properties of the Pearcey pattern while exhibiting the remarkable ability to split the mainlobe into two separate lobes.

Hermite-Gaussian-Talbot carpets.

In this Letter, we demonstrate the generation of Hermite-Gaussian-Talbot carpets (HGTC) based on the interference of a Hermite-Gaussian (HG) beam array with constant successive separation (shift). Despite the acceleration of HG beams during propagation, their symmetric structure ensures that the self-imaged carpets are generated in straight lines perpendicular to the propagation direction, at particular distances, multiples of the famous Talbot distance zT. By considering the separation as a multiple or a fraction of the Hermite-Gaussian beam width, the calculated Talbot distance zT is expressed as a function of the beam parameters, such as the Rayleigh length. The same carpets are also observed in planes situated at different fractions of zT, but with different frequency appearances. An interesting feature of these carpets is that the dimension of one cell of the beam array remains constant in each period (period fraction). We believe that such novel, to our knowledge, carpets will be useful in photonics for creating lattices and optical potentials.

Tunable spin splitting of reflected Laguerre-Gaussian beams on controllable metamaterials with anti-PT symmetry.

The intriguing photonic spin Hall effect (PSHE) of reflected Laguerre-Gaussian (LG) beams can be exhibited on the systems with optical anti-parity-time (Anti-PT) symmetry. During the reflection, the left/right circularly polarized (LCP/RCP) components of reflected LG beams are considered. By controlling parameters of the Anti-PT systems, the PSHE of reflected LCP/RCP can be identical and symmetrical with respect to incident-reflected plane (IRP). Due to gain/non-Hermitian effects of designed Anti-PT systems, special PSHE near the strong gain points (SGP) and exceptional points (EPs) can be obtained simulatively. Through analyses in PSHE of reflected LCP on four similar Anti-PT systems, specific conclusions that can even be extended to more general cases. Moreover, simulations of PSHE by simultaneously varying the incident angles * and imaginary/real dielectric constants Im/Re[ε] of the Anti-PT systems, specal PSHE and other novel optical phenomena with real applications can be revealed. So Anti-PT systems not only provide novel ways to regulate the PSHE of reflected LG beams, but also offer possibilities for new optical characteristics of devices.

Application of the Electrospinning Technique in Electrochemical Biosensors: An Overview.

Electrospinning is a cost-effective and flexible technology for producing nanofibers with large specific surface areas, functionalized surfaces, and stable structures. In recent years, electrospun nanofibers have attracted more and more attention in electrochemical biosensors due to their excellent morphological and structural properties. This review outlines the principle of electrospinning technology. The strategies of producing nanofibers with different diameters, morphologies, and structures are discussed to understand the regulation rules of nanofiber morphology and structure. The application of electrospun nanofibers in electrochemical biosensors is reviewed in detail. In addition, we look towards the future prospects of electrospinning technology and the challenge of scale production.

Biofunctionalization of dental abutments by a zinc/chitosan/gelatin coating to optimize fibroblast behavior and antibacterial properties.

Tightly sealed peri-implant gingival tissue provides a barrier against oral bacterial invasion, protecting the alveolar bone and maintaining long-term implant survival. To investigate if zinc can enhance the integration between peri-implant gingival tissue and abutment surface, we herein present novel zinc/chitosan/gelatin (Zn/CS/Gel) coatings prepared using the electrophoretic deposition (EPD) technique. The effect of these coatings on human gingival fibroblasts (hGFs) was investigated by culturing these cells on top of the EPD coatings. Surface characterization demonstrated that Zn2+ were released in a sustained and pH-responsive manner. The preclinical cell culture evaluation of these coatings indicated that the zinc-containing coatings enhanced cell migration, adhesion and collagen secretion of hGFs. Moreover, the zinc-containing coatings exhibited antibacterial efficacy by inhibiting the growth of Porphyromonas gingivalis and reducing attachment of Staphylococcus aureus. Notably, zinc-free CS/Gel coatings prevented attachment of P. gingivalis as well. The coatings were also shown to be cytocompatible with epithelial cells and osteoblasts, which are other relevant cell types which surround dental implants after clinical placement. Based on our findings, it can be concluded that Zn-containing coatings hold promise to enhance the adhesion of gingival tissue to the implant surface, which may potentially contribute to the formation of a robust peri-implant soft sealing counteracting bacterial invasion.

Spatiotemporal Airyprime complex-variable-function wave packets in a strongly nonlocal nonlinear medium.

A type of circular Airyprime function of complex-variable Gaussian vortex (AFCGV) wave packets in a strongly nonlocal nonlinear medium is introduced numerically, combining the properties of helicity states and abrupt autofocusing. We investigate the effects of the chirp factor, distribution parameter, and decay factor on the AFCGV wave packets in the strongly nonlocal nonlinear medium. Interestingly, by adjusting the distribution parameter, the AFCGV wave packets can exhibit stable rotational motions in various shapes, such as symmetric lobes and doughnuts. In addition, the Poynting vector and the gradient force of the AFCGV wave packets are also discussed. Our research not only explains the theoretical model for controlling AFCGV wave packets but also advances fundamental research on self-bending and autofocusing structured light fields.

Catalytic hairpin assembly-based AIEgen/graphene oxide nanocomposite for fluorescence-enhanced and high-precision spatiotemporal imaging of microRNA in living cells.

The low abundance, heterogeneous expression, and temporal changes of miRNA in different cellular locations pose significant challenges for both the detection sensitivity of miRNA liquid biopsy and intracellular imaging. In this work, we report an intelligently assembled biosensor based on catalytic hairpin assembly (CHA) and aggregation-induced emission (AIE), named as catalytic hairpin aggregation-induced emission (CHAIE), for the ultrasensitive detection and intracellular imaging of miRNA-155. To achieve such goal, tetraphenylethylene-N3 (TPE-N3) is used as AIE luminogen (AIEgen), while graphene oxide is introduced to quench the fluorescence. When the target miRNA is present, CHA reaction is triggered, causing the AIEgen to self-assemble with the hairpin DNA. This will restrict the intramolecular rotation of the AIEgen and produce a strong AIE fluorescence. Interestingly, CHAIE does not require any enzyme or expensive thermal cycling equipment, and therefore provides a rapid detection. Under optimal conditions, the proposed biosensor can determine miRNA in the concentration range from 2 pM to 200 nM within 30 min, with the detection limit of 0.42 pM. The proposed CHAIE biosensor in this work offers a low background signal and high sensitivity, making it applicable for highly precise spatiotemporal imaging of target miRNA in living cells.

ZnO-CeO2 Hollow Nanospheres for Selective Determination of Dopamine and Uric Acid.

ZnO-CeO2 hollow nanospheres have been successfully synthesized via the hard templating method, in which CeO2 is used as the support skeleton to avoid ZnO agglomeration. The synthesized ZnO-CeO2 hollow nanospheres possess a large electrochemically active area and high electron transfer owing to the high specific surface area and synergistic effect of ZnO and CeO2. Due to the above advantages, the resulting ZnO-CeO2 hollow spheres display high sensitivities of 1122.86 µA mM-1 cm-2 and 908.53 µA mM-1 cm-2 under a neutral environment for the selective detection of dopamine and uric acid. The constructed electrochemical sensor shows excellent selectivity, stability and recovery for the selective analysis of dopamine and uric acid in actual samples. This study provides a valuable strategy for the synthesis of ZnO-CeO2 hollow nanospheres via the hard templating method as electrocatalysts for the selective detection of dopamine and uric acid.

Propagation dynamics of self-accelerating second-order Hermite complex-variable-function Gaussian wave packets in a harmonic potential.

This paper investigates the evolutionary dynamics of self-accelerating second-order Hermite complex-variable-function Gaussian (SSHCG) wave packets in a harmonic potential. The periodic variation of the wave packets is discussed via theoretical analysis and numerical simulation. The control variables method is applied to manipulate the distribution factor, cross-phase factor, potential depth, and chirp parameter, enabling the realization of unique propagation dynamics. In three-dimensional models, the SSHCG wave packets exhibit rotational states, featuring butterfly shape, three peaks shape, two polarity shape, elliptical shape, and ring-shaped double-vortex structures. Furthermore, the energy flow and the angular momentum of the wave packets are investigated. Additionally, the performance of the radiation force on a Rayleigh dielectric particle is studied. This investigation results in the emergence of distinct SSHCG wave packet propagation dynamics, and potential applications in optical communications and optical trapping are presented.

Treatment of Coking Wastewater Using Hydrodynamic Cavitation Coupled with Fenton Oxidation Process.

Effective and economical processes for the advanced treatment of coking wastewater were urgently needed to reduce the persistent organic pollutants of external drainage. In the present work, we investigated the degradation of organic pollutants in coking wastewater through IHC/FO (imping stream hydrodynamic cavitation (IHC) coupled with the Fenton oxidation (FO) process) and IHC alone for their feasibility in the advanced treatment of coking wastewater. To select the optimum parameters, attention was paid to the effects of main operation conditions including inlet fluid pressure, medium temperature, initial pH, reaction time, and initial Fe(II) and initial H2O2 concentrations. The results showed that the effects of conditions that need energy to be maintained (such as initial pH and inlet pressure) on the organic pollutant removal efficiency through IHC/FO were less pronounced than those through IHC alone. Moreover, the application of IHC/FO could remove more organic pollutants from coking wastewater than IHC even at an energy-efficient condition. For example, the highest COD removal efficiency of 12.5% was achieved in the IHC treatment at 0.4 MPa, pH 3, and 60 min for the reaction time. In the case of IHC/FO, the maximum COD removal of 33.2% was obtained at pH 7, 0.1 MPa, 12 mmol/L H2O2, and 3 mmol/L Fe2+ after reacting for 15 min. The ultraviolet and visible spectrophotometry (UV-Vis) absorption spectra and gas chromatography and mass spectrometry (GC-MS) analysis further revealed that the kinds and amounts of pollutants (especially those that had benzenes) remaining in water treated through IHC/FO were much fewer and smaller than in water treated through IHC alone. The better performances of IHC/FO than IHC alone were likely related to the more hydroxyl radicals produced through IHC/FO. Taken together, our findings indicate that IHC/FO has great application potential in the advanced treatment of coking wastewater.

Rydberg-atom acceleration by circular Airy laser pulses.

Circular Airy pulsed beams are introduced to significantly optimize the acceleration of neutral Rydberg atoms. Compared with the conventional pulsed Gaussian beams used in the previous report, the circular Airy structure abruptly self-focuses and subsequently propagates with weak diffraction, resulting in a much higher accelerating efficiency for both radial and longitudinal velocities, as well as a longer accelerating range along the propagation axis. The parameter dependencies of the beams on the acceleration are also analyzed.

(3+1)-dimensional Pearcey-Gaussian wave packet with arbitrary velocity driven by flying focus.

The group velocity (GV) modulation of space-time wave packets (STWPs) along the transverse and longitudinal directions in free space is constrained by various factors. To surmount this limitation, a technique called "flying focus" has been developed, which enables the generation of laser pulses with dynamic focal points that can propagate at arbitrary velocities independent of GV. In this Letter, we propose a (3+1)-dimensional Pearcey-Gauss wave packet based on the "flying focus" technique, which exhibits superluminal propagation, transverse focus oscillation, and longitudinal periodic autofocusing. By selecting appropriate parameters, we can flexibly manipulate the position, the size, and the number of focal points- or make the wave packet follow a desired trajectory. This work may pave the way for the advancement of space-time structured light fields.

The Roles and Interactions of Porphyromonas gingivalis and Fusobacterium nucleatum in Oral and Gastrointestinal Carcinogenesis: A Narrative Review.

Epidemiological studies have spotlighted the intricate relationship between individual oral bacteria and tumor occurrence. Porphyromonas gingivalis and Fusobacteria nucleatum, which are known periodontal pathogens, have emerged as extensively studied participants with potential pathogenic abilities in carcinogenesis. However, the complex dynamics arising from interactions between these two pathogens were less addressed. This narrative review aims to summarize the current knowledge on the prevalence and mechanism implications of P. gingivalis and F. nucleatum in the carcinogenesis of oral squamous cell carcinoma (OSCC), colorectal cancer (CRC), and pancreatic ductal adenocarcinoma (PDAC). In particular, it explores the clinical and experimental evidence on the interplay between P. gingivalis and F. nucleatum in affecting oral and gastrointestinal carcinogenesis. P. gingivalis and F. nucleatum, which are recognized as keystone or bridging bacteria, were identified in multiple clinical studies simultaneously. The prevalence of both bacteria species correlated with cancer development progression, emphasizing the potential impact of the collaboration. Regrettably, there was insufficient experimental evidence to demonstrate the synergistic function. We further propose a hypothesis to elucidate the underlying mechanisms, offering a promising avenue for future research in this dynamic and evolving field.

A paper-based ratiometric fluorescence sensor based on carbon dots modified with Eu3+ for the selective detection of tetracycline in seafood aquaculture water.

Paper-based ratiometric fluorescence sensors are normally prepared using two or more types of fluorescent materials on a paper chip for simple, low-cost and fast detection. However, the choice of multi-step and one-step modifications on the paper chip affects the analytical performance. Herein, a novel paper-based dual-emission ratiometric fluorescence sensor was designed for the selective detection of tetracycline (TC). Carbon dots (CDs) modified with Eu3+ were combined with a sealed paper-based microfluidic chip by two methods: one-step grafting of CDs-Eu3+ on paper and step-by-step grafting of CDs and Eu3+ on paper. The analytical performance was studied and optimized respectively. The red fluorescence of Eu3+ at 450 nm is enhanced and the blue fluorescence of CDs at 617 nm is quenched by energy transfer in the presence of TC. Under optimal conditions, TC is selectively determined in the linear range from 0.1 µM to 100 µM with a detection limit of 0.03 µM by the step-by-step grafting method. In addition, the sealed paper chip could effectively prevent pollution and volatilization from the reagent. This technique has been used to analyze TC in seafood aquaculture water with satisfactory results.

Propagation dynamics of the controllable circular Airyprime beam in the Kerr medium.

In this paper, we study the propagation dynamics of the circular Airyprime beam (CAPB) in the Kerr medium for the first time. We investigate the effects of the astigmatism factor, the chirp factor, and vortices on the CAPB propagating in the Kerr medium. At the same time, we are also introducing a special-shaped Airyprime beam (SAPB) during its propagation. The transmission characteristics of the CAPB and the SAPB in the Kerr medium are compared under identical conditions. Our theoretical results provide additional possibilities for CAPB modulation in the Kerr medium, thereby promising wider applicability of CAPB in various research areas.

Highly efficient enhancement and extension of focusing ability for ring Pearcey beam by means of dual-region parabolic trajectory offset modulation.

It is a highly significant area of research to investigate how to effectively enhance the focusing ability of abruptly auto-focusing beams (AAFBs) while extending the focal length. We introduce a dual-region parabolic trajectory offset modulation to auto-focusing ring Pearcey beams (RPBs), presenting a novel, to the best of our knowlege, approach to extend the focal length while greatly enhancing their auto-focusing capabilities. Unlike directly introducing a linear chirp, which inevitably shortens the focal length to enhance the auto-focusing ability and allows only single focusing in the RPBs, our scheme can achieve a multi-focusing effect. Furthermore, we have experimentally generated such a beam, verifying our theoretical predictions. Our findings offer promising possibilities for generating optical bottles, trapping multiple particles periodically, and enhancing free-space optical communication capabilities.

Oral Bacteria: Friends and Foes?

The oral cavity is an ideal niche for microbial prosperity due to its stable temperature, suitable pH, and continuous nutrient supply [...].

Singular multi-wavelength and multi-waveband transparencies generated by P T-symmetric dumbbell optical waveguide networks.

In this paper, we investigate the singular multi-wavelength and multi-waveband transparencies generated by P T-symmetric dumbbell optical waveguide networks composed of two materials, and obtain the number regularity for the transparency wavelengths of one-unit-cell system and the general relationships for the transmission and reflection coefficients of multi-unit-cell systems. Consequently, three types of exact transparencies produced by multi-unit-cell systems are found based on the aforementioned formulas: (i)exact multi-wavelength unidirectional or bidirectional transparency as the same as those of one-unit-cell system; (ii)exact multi-wavelength bidirectional transparency at which one-unit-cell system cannot produce exact transparency, generated by adjusting the number of unit cells; (iii)exact multi-wavelength bidirectional transparency at which one-unit-cell system produces exact transparency, also generated by adjusting the number of unit cells. It provides theoretical foundations for developing highly sensitive and multi-wavelength optical filters. On the other hand, we also discover that multi-unit-cell systems can create approximate multi-waveband bidirectional transparencies by adjusting the number of unit cells, which provides scientific support for developing high-performance optical stealth devices.