Enhancing RABASAR for Multi-Temporal SAR Image Despeckling through Directional Filtering and Wavelet Transform.

The presence of speckle noise severely hampers the interpretability of synthetic aperture radar (SAR) images. While research on despeckling single-temporal SAR images is well-established, there remains a significant gap in the study of despeckling multi-temporal SAR images. Addressing the limitations in the acquisition of the "superimage" and the generation of ratio images within the RABASAR despeckling framework, this paper proposes an enhanced framework. This enhanced framework proposes a direction-based segmentation approach for multi-temporal SAR non-local means filtering (DSMT-NLM) to obtain the "superimage". The DSMT-NLM incorporates the concept of directional segmentation and extends the application of the non-local means (NLM) algorithm to multi-temporal images. Simultaneously, the enhanced framework employs a weighted averaging method based on wavelet transform (WAMWT) to generate superimposed images, thereby enhancing the generation process of ratio images. Experimental results demonstrate that compared to RABASAR, Frost, and NLM, the proposed method exhibits outstanding performance. It not only effectively removes speckle noise from multi-temporal SAR images and reduces the generation of false details, but also successfully achieves the fusion of multi-temporal information, aligning with experimental expectations.

Optimization of Signal Timing for Urban Expressway Exit Ramp Connecting Intersection.

To alleviate the traffic problems of congestion and queue overflow on a mainline at the intersection of an urban expressway exit ramp articulation during peak hours, a bi-level programming optimization model of signal timing is proposed. The lower-level optimization objective is to maximize the capacity of the expressway exit ramp that articulates with the entrance road, while the upper-level optimization objective is to minimize the average vehicle delay and the number of stops per vehicle, taking into account the queue length in the direction of the ramp and other directions. The particle swarm optimization algorithm is selected to solve the proposed model, applied to a real case, and is validated using MATLAB and VISSIM simulation platforms. The simulation results show that the average vehicle delay and the number of stops per vehicle in the exit ramp on the expressway are reduced by 22.09% and 18.60%, while those in the intersection area are reduced by 20.96% and 17.19%, respectively. The conclusion indicates that the signal timing scheme obtained by this method can effectively improve the traffic efficiency at the intersection of the exit ramp on the expressway and alleviate the problem of congestion and the overflow of the exit ramp back to the mainline.

Pulmonary Hypertension Caused by Fibrosing Mediastinitis.

Pulmonary hypertension (PH) is a progressive and severe disorder in pulmonary hemodynamics. PH can be fatal if not well managed. Fibrosing mediastinitis (FM) is a rare and benign fibroproliferative disease in the mediastinum, which may lead to pulmonary vessel compression and PH. PH caused by FM (PH-FM) is a pathologic condition belonging to group 5 in the World Health Organization PH classification. PH-FM has a poor prognosis because of a lack of effective therapeutic modalities and inappropriate diagnosis. With the development of percutaneous pulmonary vascular interventional therapy, the prognosis of PH-FM has been greatly improved in recent years. This article provides a comprehensive review on the epidemiology, pathophysiologic characteristics, clinical manifestations, diagnostic approaches, and treatment modalities of PH-FM based on data from published reports and our medical center with the goal of facilitating the diagnosis and treatment of this fatal disease.

An STP-HSI index method for urban built-up area extraction based on multi-source remote sensing data.

A change in an urban built-up area can reflect the process of urbanization and the development of a city. At present, multi-source remote sensing data extraction of built-up areas based on the human settlement index (HSI) has achieved relatively good results but the existence of noise, such as light spillover in the night-time light remote sensing data, seriously affects the accuracy of the HSI. In this paper, a high-precision human settlement index (STP-HSI) method based on spatio-temporal remote sensing and point-of-interest (POI) data is presented to improve the classification accuracy in urban built-up areas extractions. First, to correct light spillover, a new night-time light index the fuzzy c-means spatio-temporal point (FCM-STP) based on fuzzy c-means clustering is proposed, which integrates the spatio-temporal characteristics and uses night light video imaging data from Luojia-1 and POI data. Then, based on the FCM-STP index, the HSI is updated to the STP-HSI index. Finally, a random forest algorithm is used to extract the urban built-up areas, and the random forest feature database is composed of normalized difference vegetation index (NDVI), normalized difference built-up index (NDBI) and STP-HSI index features and texture features. To develop and evaluate the accuracy of the new method for built-up areas extraction with multi-source data, three test sites located in the cities of China (Guangzhou, Xiamen and Nanjing) are used. The experimental results show that our method outperforms the single-source multi-spectral (Landsat 8) data extraction results, the overall accuracy is improved by up to 7.52%, and the kappa coefficient is improved by up to 14%. Compared with the HSI index, the maximum contribution rates of the STP-HSI increased by 25.74%. These experimental results show that the method in this paper is feasible.

Comprehensive metrological and content analysis of the income inequality research in health field: A bibliometric analysis.

The association between income inequality in a society and the poor health status of its people has attracted the attention of researchers from multiple disciplines. Based on the ISI Web of Science database, bibliometric methods were used to analyze 546 articles related to income inequality research in health field published between 1997 and 2021. We found that the USA contributed most articles, the Harvard Univ was the most influential institution, Social Science & Medicine was the most influential journal, and Kawachi I was the most influential author; the main hotspots included the income inequality, income, health inequality, mortality, socioeconomic factors, concentration index, social capital, self-rated health, income distribution, infant mortality, and population health in 1997-2021; the cardiovascular disease risk factor, social capital income inequality, individual mortality risk, income-related inequalities, understanding income inequalities, income inequality household income, and state income inequality had been the hot research topics in 1997-2003; the self-assessed health, achieving equity, income-related inequalities, oral health, mental health, European panel, occupational class, and cardiovascular diseases had been the hot research topics in 2004-2011; the adolescent emotional problem, South Africa, avoidable mortality, rising inequalities, results from world health survey, working-age adult, spatial aggregation change, prospective study, and mental health-empirical evidence had been the hot research topics in 2012-2021; there were 11 articles with strong transformation potential during 2012-2021. The research results of this paper are helpful to the scientific understanding of the current status of income inequality research in health field.

Trends, Issues and Future Directions of Urban Health Impact Assessment Research: A Systematic Review and Bibliometric Analysis.

Health impact assessment (HIA) has been regarded as an important means and tool for urban planning to promote public health and further promote the integration of health concept. This paper aimed to help scientifically to understand the current situation of urban HIA research, analyze its discipline co-occurrence, publication characteristics, partnership, influence, keyword co-occurrence, co-citation, and structural variation. Based on the ISI Web database, this paper used a bibliometric method to analyze 2215 articles related to urban HIA published from 2012 to 2021. We found that the main research directions in the field were Environmental Sciences and Public Environmental Occupational Health; China contributed most articles, the Tehran University of Medical Sciences was the most influential institution, Science of the Total Environment was the most influential journal, Yousefi M was the most influential author. The main hotspots include health risk assessment, source appointment, contamination, exposure, particulate matter, heavy metals and urban soils in 2012-2021; road dust, source apposition, polycyclic aromatic hydrocarbons, air pollution, urban topsoil and the north China plain were always hot research topics in 2012-2021, drinking water and water quality became research topics of great concern in 2017-2021. There were 25 articles with strong transformation potential during 2020-2021, but most papers carried out research on the health risk assessment of toxic elements in soil and dust. Finally, we also discussed the limitations of this paper and the direction of bibliometric analysis of urban HIA in the future.

Occurrence, seasonal variation and environmental impact of phosphorus flame retardants in a large scale wastewater treatment plant.

The occurrence, seasonal variation and emission of nine widely used phosphorus flame retardants (PFRs) were investigated in a wastewater treatment plant (WWTP) located in Guangzhou, China, over 1 year. Results showed that PFRs were widely detected in wastewater and sewage sludge. Tris(2-chloroisopropyl) phosphate (TCIPP) was the most dominant PFRs in influent, effluent, and sludge. Significant seasonal variation of total PFRs in the influent was observed (p < 0.05). However, no significant seasonal variation found in chlorinated and alkyl PFRs. The emission of PFRs was comparable with the previously reported values of decabromodiphenyl ether in WWTPs. Risk quotient for PFRs showed low eco-toxicity risk in effluent for aquatic organisms. Since the removal efficiency of total PFRs was less than 30% and the use of PFRs had been increasing, continuous monitoring of the environmental impact on the receiving water is still needed.

Geometric Self-Calibration of YaoGan-13 Images Using Multiple Overlapping Images.

Geometric calibration is an important means of improving the absolute positioning accuracy of space-borne synthetic aperture radar imagery. The conventional calibration method is based on a calibration field, which is simple and convenient, but requires a great deal of manpower and material resources to obtain ground control points. Although newer cross-calibration methods do not require ground control points, calibration accuracy still depends on a periodically updated reference image. Accordingly, this study proposes a geometric self-calibration method based on the positioning consistency constraint of conjugate image points to provide rapid and accurate calibration of the YaoGan-13 satellite. The proposed method can accurately calibrate geometric parameters without requiring ground control points or high-precision reference images. To verify the absolute positioning accuracy obtained using the proposed self-calibration method, YaoGan-13 Stripmap images of multiple regions were collected and evaluated. The results indicate that high-accuracy absolute positioning can be achieved with a plane accuracy of 3.83 m or better for Stripmap data, without regarding elevation error. Compared to the conventional calibration method using high-accuracy control data, the difference between the two methods is only about 2.53 m, less than the 3-m resolution of the image, verifying the effectiveness of the proposed self-calibration method.

Assessing pollution and risk of polycyclic aromatic hydrocarbons in sewage sludge from wastewater treatment plants in China's top coal-producing region.

Managing and disposing of sewage sludge have been a severe environmental challenge around the world. China produces hundreds of million tons of sewage sludge annually, and a better understanding of the extent and risk of the associated pollution is of critical importance for implementing environmentally safe regulations and practices. The present study examined the quantity, composition, source, and risk of polycyclic aromatic hydrocarbons (PAHs) in sewage sludge from 18 wastewater treatment plants (WWTPs) in Shaanxi, one of China's top coal-producing provinces. The total concentrations of 16 PAHs varied from 778 to 3264 ng/g dry weight, which is below the upper safety limit (5000 ng/g dry weight) set for the disposal of sludge from municipal wastewater treatment plants for agricultural use in China. However, the concentration of individual PAH compound exceeded the acceptable level prescribed by the Netherland Soil Standard. Three-ring PAHs were the most abundant constituent (50% of total PAHs on average), followed by four-ring PAHs averaging 25%. Relative to sludge PAHs in the same region a decade ago, the total concentrations decreased by more than 27% and the composition shifted to a more pronounced dominance by low molecular weight compounds. This compositional shift suggests higher contributions of petrogenic sources, which may reflect China's increasing consumption of petroleum products over the past decade. The flux of sludge PAHs from each WWTP was positively correlated with the corresponding city's GDP and population, and the total flux amounted to over 100 kg each year for WWTPs in the Xi'an city. The mean toxicity equivalent quantity (TEQ) value was more than twice higher than the value recommended by the Netherlands Soil Standard, and seven carcinogenic PAHs were the primary contributor (i.e., 89-99%) of the TEQ. Collectively, our findings demonstrate that sewage sludge PAHs in Shaanxi constitute a significant source of environmental pollution and toxicity, which cautions against the direct discharge and reuse of sewage sludge and further highlights challenges in managing and disposing of the vast quantities of sewage sludge in China.

Block Adjustment without GCPs for Chinese Spaceborne SAR GF-3 Imagery.

The Gaofen-3 (GF-3) satellite is the first C-band multi-polarization synthetic aperture radar (SAR) with the ability of high-accuracy mapping in China. However, the Ground Control Points (GCPs) are essential to ensure the accuracy of mapping for GF-3 SAR imagery at present. In this paper, we analyze the error sources that affect the geometric processing and propose a new block adjustment method without GCPs for GF-3 SAR imagery. Firstly, the geometric calibration of GF-3 image is carried out. Secondly, the rational polynomial coefficient (RPC) model is directly generated after the geometric calibration parameters compensation of each image. Finally, we solve the orientation parameters of the GF-3 images through DEM assisted planar block adjustment and conduct ortho-rectification. With two different imaging modes of GF-3 satellite, which include the QPSI and FS2, we carry out the block adjustment without GCPs. Experimental results of testing areas including Wuhan city and Hubei province in China show that the geometric mosaic accuracy and the absolute positioning accuracy of the orthophoto are better than one pixel, which has laid a good foundation for the application of GF-3 image in global high-accuracy mapping.

Organophosphorus flame retardants and heavy metals in municipal landfill leachate treatment system in Guangzhou, China.

The occurrence, distribution and removal efficiencies of organophosphorus flame retardants (OPFRs) and metals were examined in a municipal landfill leachate treatment system in Guangzhou, China. Five OPFRs and thirty-five metals were detected in wastewater samples collected at different treatment stages. ∑OPFRs was reduced from 4807.02 ng L-1 to 103.91 ng L-1 through the treatment system, with close to 98% removed from the dissolved phase. Tris(clorisopropyl) phosphates (TCPPs) dominated through the treatment process and accounted for over 80% and 50% of ∑OPFRs at the influent and the effluent, respectively. TCPPs were most efficiently removed (98.6%) followed by tris(2-chloroethyl) phosphate (TCEP) (96.6%) and triphenyl phosphate (TPP) (88.5%). For metals, Fe, Cr, and Rb were dominant in the raw leachate, detected at 7.55, 2.82, and 4.50 mg L-1, respectively. Thirteen regulated heavy metals - including eight major pollutants (i.e., As. Cd, Cr, Cu, Hg, Ni, Pb, and Zn) - have been detected in all wastewater samples at sub-mg L-1 levels. Over 99.5% removal was achieved for Cr, Ni, and Fe, and close to 95% removal efficiency was observed for Rb. For the eight major heavy metals, over 99% removal was observed; the only exception was Cu, which was removed at 89%. It was found that microfiltration/reverse osmosis was critical for the removal of OPFRs and heavy metals while the core biological treatment played a minor role towards their removal. Remobilization of Co, Cu, Fe, Hg, Mn, Ni, Sb, and Sr from the returned sludge occurred during the second denitrification, indicating the need for additional post-biological process for effective removal of both contaminants. This study highlights the critical need to develop cheap, effective treatment technologies for contaminants-laden leachate generated from open dumps and under-designed landfills.

Improvement of Gaofen-3 Absolute Positioning Accuracy Based on Cross-Calibration.

The Chinese Gaofen-3 (GF-3) mission was launched in August 2016, equipped with a full polarimetric synthetic aperture radar (SAR) sensor in the C-band, with a resolution of up to 1 m. The absolute positioning accuracy of GF-3 is of great importance, and in-orbit geometric calibration is a key technology for improving absolute positioning accuracy. Conventional geometric calibration is used to accurately calibrate the geometric calibration parameters of the image (internal delay and azimuth shifts) using high-precision ground control data, which are highly dependent on the control data of the calibration field, but it remains costly and labor-intensive to monitor changes in GF-3's geometric calibration parameters. Based on the positioning consistency constraint of the conjugate points, this study presents a geometric cross-calibration method for the rapid and accurate calibration of GF-3. The proposed method can accurately calibrate geometric calibration parameters without using corner reflectors and high-precision digital elevation models, thus improving absolute positioning accuracy of the GF-3 image. GF-3 images from multiple regions were collected to verify the absolute positioning accuracy after cross-calibration. The results show that this method can achieve a calibration accuracy as high as that achieved by the conventional field calibration method.

Multi-Mode GF-3 Satellite Image Geometric Accuracy Verification Using the RPC Model.

The GaoFen-3 (GF-3) satellite is the first C-band multi-polarization synthetic aperture radar (SAR) imaging satellite with a resolution up to 1 m in China. It is also the only SAR satellite of the High-Resolution Earth Observation System designed for civilian use. There are 12 different imaging models to meet the needs of different industry users. However, to use SAR satellite images for related applications, they must possess high geometric accuracy. In order to verify the geometric accuracy achieved by the different modes of GF-3 images, we analyze the SAR geometric error source and perform geometric correction tests based on the RPC model with and without ground control points (GCPs) for five imaging modes. These include the spotlight (SL), ultra-fine strip (UFS), Fine Strip I (FSI), Full polarized Strip I (QPSI), and standard strip (SS) modes. Experimental results show that the check point residuals are large and consistent without GCPs, but the root mean square error of the independent checkpoints for the case of four corner control points is better than 1.5 pixels, achieving a similar level of geometric positioning accuracy to that of international satellites. We conclude that the GF-3 satellite can be used for high-accuracy geometric processing and related industry applications.

Removal Efficiency and Risk Assessment of Polycyclic Aromatic Hydrocarbons in a Typical Municipal Wastewater Treatment Facility in Guangzhou, China.

The loading and removal efficiency of 16 US EPA polycyclic aromatic hydrocarbons (PAHs) were examined in an inverted A²/O wastewater treatment plant (WWTP) located in an urban area in China. The total PAH concentrations were 554.3 to 723.2 ng/L in the influent and 189.6 to 262.7 ng/L in the effluent. The removal efficiencies of ∑PAHs in the dissolved phase ranged from 63 to 69%, with the highest observed in naphthalene (80% removal). Concentration and distribution of PAHs revealed that the higher molecular weight PAHs became more concentrated with treatment in both the dissolved phase and the dewatered sludge. The sharpest reduction was observed during the pretreatment and the biological phase. Noncarcinogenic risk, carcinogenic risk, and total health risk of PAHs found in the effluent and sewage sludge were also assessed. The effluent BaP toxic equivalent quantities (TEQBaP) were above, or far above, standards in countries. The potential toxicities of PAHs in sewage effluent were approximately 10 to 15 times higher than the acceptable risk level in China. The health risk associated with the sewage sludge also exceeded international recommended levels and was mainly contributed from seven carcinogenic PAHs. Given that WWTP effluent is a major PAH contributor to surface water bodies in China and better reduction efficiencies are achievable, the present study highlights the possibility of utilizing WWTPs for restoring water quality in riverine and coastal regions heavily impacted by PAHs contamination.

Geometric Calibration and Accuracy Verification of the GF-3 Satellite.

The GF-3 satellite is the first multi-polarization synthetic aperture radar (SAR) imaging satellite in China, which operates in the C band with a resolution of 1 m. Although the SAR satellite system was geometrically calibrated during the in-orbit commissioning phase, there are still some system errors that affect its geometric positioning accuracy. In this study, these errors are classified into three categories: fixed system error, time-varying system error, and random error. Using a multimode hybrid geometric calibration of spaceborne SAR, and considering the atmospheric propagation delay, all system errors can be effectively corrected through high-precision ground control points and global atmospheric reference data. The geometric calibration experiments and accuracy evaluation for the GF-3 satellite are performed using ground control data from several regions. The experimental results show that the residual system errors of the GF-3 SAR satellite have been effectively eliminated, and the geometric positioning accuracy can be better than 3 m.

Occurrence of PBDEs and alternative halogenated flame retardants in sewage sludge from the industrial city of Guangzhou, China.

This study investigated the prevalence and abundance of halogenated flame retardants (HFRs) in sludge samples from 5 sewage treatment plants in Guangzhou, China. Detection of 18 polybrominated diphenyl ethers (PBDEs), 9 alternative HFRs including Dechlorane Plus (DP), brominated alkylbenzenes, and polybrominated biphenyls, and 2 related degradation products was conducted. Decabromodiphenyl ether (BDE 209) and decabromodiphenyl ethane (DBDPE) were the dominant HFRs, with concentrations ranging from 200 to 2150 ng/g and 680-27,400 ng/g, respectively. The DBDPE detected was the highest level reported so far, exceeding those previously reported by 10-100 times. PBDEs were surpassed as the dominant HFRs in sewage sludge, with mean DBDPE/BDE 209 ratio exceeding 2 in all samples. The review of earlier surveys reveals that DBDPE level was surging while BDE 209 was declining. Annual emissions of BDE 209, DP, and DBDPE were estimated to be 227.9, 10.5, and 979.3 kg/yr, respectively. Although ecological risks assessment suggested low risks for the examined sludge, the key environmental properties and transformation pathways of alternative HFRs remain largely unknown. These findings prompt for further investigations on alternative HFR and sustainable management practices for HFR-laden biosolids. The HFR emission pattern revealed in this study is likely representative of other similarly industrialized regions in the post-PBDE era.

Serum Bactericidal Assay: New Role in Salmonella Detection.

While inspecting animal feed for Salmonella contamination, we routinely observed bacterial colonies on selective agars that were similar in appearance to those formed by Salmonella. These were identified as Citrobacter freundii, Proteus mirabilis, and Serratia fonticola using biochemical and serological techniques. Because the presence of these bacterial species confounds identification of Salmonella, we refer to them as "interference bacteria." Polyvalent antisera against these interference bacteria were prepared by immunizing rabbits with a mixture of all three organisms. To minimize or eliminate interference by these bacteria, the polyvalent antisera were introduced between the steps of selective enrichment and Salmonella-selective plating. The antisera raised against the interference bacteria, when combined with neonatal rabbit complement, exhibited specific bactericidal activity against C. freundii, P. mirabilis, and S. fonticola. The respective serum bactericidal assay titers were 2(9), 2(8), and 2(10). In selective broth, polyvalent antisera could also kill the target bacterial cells effectively. We tested 526 samples (186 white fishmeal, 97 red fishmeal, and 243 cattle bone powder) using the polyvalent antisera and found that the rates of contamination of each species of the three respective foods decreased by 58.8, 100, and 83%. Our data indicates that polyvalent sera against C. freundii, P. mirabilis, and S. fonticola can be used as inhibitors to increase the accuracy of Salmonella detection.

First report of zoonotic Cryptosporidium spp., Giardia intestinalis and Enterocytozoon bieneusi in golden takins (Budorcas taxicolor bedfordi).

Genetic study of Cryptosporidium spp., Giardia intestinalis and Enterocytozoon bieneusi at species/assemblage/genotype/subtype level facilitates understanding their mechanical transmissions and underpins their control. A total of 191 fresh faecal samples were collected from golden takins in China and examined using multilocus sequence typing (MLST). Cryptosporidium spp. was detected in 15 faecal samples (7.9%), including Cryptosporidium parvum (2/15) and Cryptosporidium andersoni (13/15). MLST tool identified C. andersoni subtypes (A1, A4, A4, A1) and (A4, A4, A4, A1), and C. parvum gp60 gene subtype IId A19G1. The prevalence of G. intestinalis infection was 8.9% (17/191) and assemblage analysis identified 14 assemblage E and three assemblage B. Intra-variations were observed at triose phosphate isomerase (tpi), beta giardin (bg) and glutamate dehydrogenase (gdh) loci within the assemblage E, showing seven, three and three new subtypes in respective locus. Ten and one multilocus genotypes (MLGs) were present in assemblages E and B, respectively. E. bieneusi infection was positive in 14.7% (28/191) of the examined specimens, with three genotypes known (BEB6, D and I) and four novel internal transcribed spacer (ITS) genotypes (TEB1-TEB4). The present study revealed, for the first time, the presence of zoonotic C. parvum IId A19G1, G. intestinalis assemblage B and E. bieneusi genotype D and four novel genotypes in golden takins in China. These findings expand the host range of three zoonotic pathogens and have important implications for controlling cryptosporidiosis, giardiasis and microsporidiosis in humans and animals.

Fuzzy State Transition and Kalman Filter Applied in Short-Term Traffic Flow Forecasting.

Traffic flow is widely recognized as an important parameter for road traffic state forecasting. Fuzzy state transform and Kalman filter (KF) have been applied in this field separately. But the studies show that the former method has good performance on the trend forecasting of traffic state variation but always involves several numerical errors. The latter model is good at numerical forecasting but is deficient in the expression of time hysteretically. This paper proposed an approach that combining fuzzy state transform and KF forecasting model. In considering the advantage of the two models, a weight combination model is proposed. The minimum of the sum forecasting error squared is regarded as a goal in optimizing the combined weight dynamically. Real detection data are used to test the efficiency. Results indicate that the method has a good performance in terms of short-term traffic forecasting.

Endoplasmic reticulum stress-mediated apoptosis is activated in intestines of mice with Trichinella spiralis infection.

Gastrointestinal helminth infection, including Trichinella spiralis, initiates a series of intestinal structural, cellular and physiological changes. Intestinal invasion is an important stage of trichinellosis because it determines the development and subsequent course of the disease and its consequences. Apoptosis mediated by endoplasmic reticulum stress (ERS) plays a key role in infectious diseases, but the effect of T. spiralis infection on inducing apoptosis in the small intestine has been neglected. We investigated apoptosis and changes in ERS-associated apoptosis molecules in the intestine of mice with T. spiralis infection. TUNEL staining and detection of the apoptotic marker cleaved caspase 3 revealed that apoptosis occurred in the mouse intestine at days 3 and 7 post-infection. The ER chaperone 78-kDa glucose-regulated protein (GRP78) was upregulated at days 3 and 7 post-infection. The ERS-associated apoptosis molecules C/EBP homologous protein, cleaved caspase 12 and c-Jun NH2-terminal kinase were upregulated at days 3 and 7, days 3, 7 and 10 and days 7 and 10 post-infection, respectively. Thus, apoptosis occurred in the intestine of mice with T. spiralis infection, and the ERS-mediated apoptosis pathway was activated by infection with this small intestine dwelling nematode.