ABSTRACT
AIM: To study the efficacy of cytoflavin in correction of oxidative stress and the activity of Na/K-ATPase in posttraumatic craniocerebral trauma (CCT) in rats. MATERIAL AND METHODS: The study was performed on white non-linear female rats with a modeled CCT. An effect of cytoflavin on free radical oxidation and plasma antioxidant system, processes of plasma lipid peroxidation and antioxidant properties of red blood cells and erythrocyte Na/KATPases activity was studied on 1, 3, 7- and 12th day after CCT. RESULTS AND CONCLUSION: CCT identified the growth of free-radical processes in blood plasma and an increase in lipid peroxidation in plasma and red blood cells as well as a reduction in the activity of catalase and Na/K-ATPase. Introduction of cytoflavin reduced oxidative stress from the 3rd day and normalized free radical oxidation and lipid peroxidation in plasma and red blood cells to the 7th day of the study against the background of the high total antioxidant capacity of blood and erythrocyte catalase activity. The effect of cytoflavin led to an increase in the activity of Na/K-ATPase on the 3rd-7th day of the study compared with the reduced activity of the enzyme in the control. Recovery of pro- and antioxidant balance under the action of cytoflavin associated with increased activity of Na/K-ATPase aimed at optimizing and reducing the homeostatic mechanisms of secondary damage in the post traumatic period.
Subject(s)
Antioxidants/pharmacology , Brain Injuries, Traumatic/blood , Brain Injuries, Traumatic/drug therapy , Erythrocytes/enzymology , Flavin Mononucleotide/pharmacology , Inosine Diphosphate/pharmacology , Niacinamide/pharmacology , Oxidative Stress/drug effects , Sodium-Potassium-Exchanging ATPase/metabolism , Succinates/pharmacology , Animals , Antioxidants/therapeutic use , Catalase/metabolism , Disease Models, Animal , Drug Combinations , Female , Flavin Mononucleotide/therapeutic use , Inosine Diphosphate/therapeutic use , Lipid Peroxidation/drug effects , Niacinamide/therapeutic use , Rats , Succinates/therapeutic useABSTRACT
It is established that exposure of the red blood cells of the rats to low doses of ionizing radiation (0.04; 0.08; 0.16; 0.25 and 0.33 mGr) leads to non-linear changes in the processes of lipid peroxidation in the membrane of erythrocytes, their electrophoretic mobility, osmotic resistance. In a dose range from 0.08 to 0.16 mGr with ionizing radiation apoptosis that determines a temporary slowing of the process of hemolysis and stabilization of erythrocytes that is confirmed by morphological changes in erythrocytes is most likely triggered.
Subject(s)
Apoptosis/radiation effects , Erythrocytes/chemistry , Gamma Rays , Hemolysis/radiation effects , Animals , RatsABSTRACT
In this paper, we study the relation between the electrophoretic mobility of erythrocytes (EPME) and the activity of Na,K-ATPase of erythrocytes under the action of different types of stress factors (immobilization, hypoxia, radiation exposure), as well as the introduction of stress implementing agents (adrenaline, cortisol, bee and toad venom) rats. Activity of Na,K-ATPase and the change of EPME washed erythrocytes were examined through 15, 120 min and 24 hours after exposure. Found that at the initial stage (15 min after a stress-exposure) decrease of EPME associated with increased enzyme activity and further increase of EPME takes place on the background of the decrease of activity of Na,K-ATPase in all types of alterations, except the actions toad venom and cortisol. Toad venom and cortisol led to reduced activity of Na,K-ATPase and increase of EPME.
Subject(s)
Erythrocytes/cytology , Hypoxia/enzymology , Sodium-Potassium-Exchanging ATPase/metabolism , Amphibian Venoms/pharmacology , Animals , Animals, Outbred Strains , Bee Venoms/pharmacology , Cells, Cultured , Electrophoresis , Epinephrine/pharmacology , Erythrocytes/drug effects , Erythrocytes/enzymology , Erythrocytes/radiation effects , Female , Gamma Rays , Hydrocortisone/pharmacology , Hypoxia/physiopathology , Immobilization , Oxygen/pharmacology , Rats , Sodium-Potassium-Exchanging ATPase/antagonists & inhibitors , Stress, Physiological/drug effects , Stress, Physiological/radiation effects , Time FactorsABSTRACT
The article deals with the results of analysis of electrophoretic mobility of erythrocytes and leukogram in patients with dyscirculatory encephalopathy on different stages of disease on application therapy with inclusion of stress-modulating pharmaceuticals into course of treatment. It is established that the electrophoretic mobiliy of erythrocytes makes it possible to evaluate the adaptive indicators blood in patients with dyscirculatory encephalopathy. The consideration of these indicators makes feasible the substantiation of inclusion of stress-modulating therapy into complex treatment of patients with chronic cerebrovascular inefficiency.
Subject(s)
Brain Ischemia , Chronic Disease , Erythrocytes , Adult , Brain Ischemia/blood , Brain Ischemia/drug therapy , Delta Sleep-Inducing Peptide/administration & dosage , Drug Combinations , Erythrocytes/chemistry , Erythrocytes/drug effects , Flavin Mononucleotide/administration & dosage , Glycine/administration & dosage , Humans , Inosine Diphosphate/administration & dosage , Middle Aged , Niacinamide/administration & dosage , Succinates/administration & dosageABSTRACT
It has been shown that the exposure of "intact" rats and human blood to low-intensity pulse magnetic field leads to similar changes in the electrophoretic mobility of erythrocytes, which may be connected with the modification of the spectrum of their membrane proteins.
Subject(s)
Electromagnetic Fields , Erythrocytes/radiation effects , Animals , Blood Proteins/metabolism , Digestive System Diseases/blood , Electrophoresis , Erythrocyte Membrane/metabolism , Erythrocytes/physiology , Female , Humans , In Vitro Techniques , Magnetics , Membrane Proteins/metabolism , RatsABSTRACT
It is carried out research of action low-intensive electromagnetic radiations--low-intensive laser radiation and radiations of the highest frequency on normal animals and at modelling the stress-reaction, caused by introduction of adrenaline. Absence of effects of system of blood is noted at action low-intensive electromagnetic radiations on normal an organism and them correction action on alteration an organism, shown in restoration of the broken parameters--leukocyte the blood count, electrophoretic mobility of erythrocytes and phospholipide's structure of their membranes.
Subject(s)
Erythrocytes/radiation effects , Lasers , Leukocytes/radiation effects , Microwaves , Animals , Electrophoresis , Erythrocytes/physiology , Female , Leukocyte Count , Leukocytes/cytology , Rats , Stress, Physiological/radiation effectsABSTRACT
Clinical and experimental studies were under way to examine nonspecific adaptive reactions of blood in chronic brain ischemia. The electrophoretic mobility of red blood cells and differential blood count were studied in animals (rats) when simulating local and total brain ischemia, as well as in patients with stages I, II, and III dyscirculatory encephalopathy. General mechanisms responsible for changing the adaptive reactions of blood were found in varying chronic brain ischemia in the experiments and clinical observations. These characterize the activity of the pituitary-adrenal system and the state of an adaptive process in the body.
Subject(s)
Brain Ischemia/blood , Pituitary-Adrenal System/metabolism , Adult , Animals , Chronic Disease , Female , Humans , Male , Middle Aged , Rats , Rats, WistarABSTRACT
Changes in the electrophoretic mobility of red blood cells and their phospholipid composition were studied in patients with psoriasis and duodenal peptic ulcer. A relationship of the electrophoretic mobility of red blood cells to their phospholipids spectrum was established in the above diseases. The paper shows the same change in the electrophoretic mobility of red blood cells and a change in the fractions of phosphotidylethanolamine, sphingomyelin, lysophosphotidylcholine of the erythrocytic membranes.
Subject(s)
Duodenal Diseases/blood , Erythrocyte Membrane/metabolism , Phospholipids/blood , Psoriasis/blood , Electrophoresis , Erythrocyte Membrane/chemistry , Female , Humans , Male , Phospholipids/analysisABSTRACT
The effect of neuraminidase, trypsin and pronase on the electrophoretic mobility of human erythrocytes and their aggregation induced by La3+ was studied in vitro. The aggregation of intact red cells differs from that of cells treated with neuraminidase and proteolytic enzymes. The above differences are observed in the range of La3+ suspension concentration from 20 to 330 microM. A possible mechanism of aggregation is discussed.
Subject(s)
Erythrocytes/physiology , Lanthanum/metabolism , Neuraminidase/metabolism , Pronase/metabolism , Trypsin/metabolism , Cell Aggregation , Electricity , Electrophoresis , Erythrocytes/metabolism , Humans , In Vitro TechniquesABSTRACT
The change of electrophoretic mobility (EPM) of erythrocytes of donor blood caused by their incubations with the bee venom and its major components--melittin and phospholipase A2 was investigated. It was estimated that the bee venom reversibly decreased EPM of erythrocytes to the higher degree than its separate components. The reaction was completely abolished after preliminary processing of erythrocytes with neuroaminidase and partly after their processing with glutaraldehyde, but the effect of EPM increased under the conditions of the increasing of ATP concentration. Proceeding from the authors' data it was concluded that the major components of the bee venom successively interacted with glicocalyx elements and membrane of erythrocytes which evokes the charge redistribution in these blood cells.
