ABSTRACT
We studied the possibility of using liposomal forms of hydroxyaluminium tetra-3-phenylthiophthalocyanine as a near infrared band photosensitizer. Experiments on mice with solid Ehrlich tumor and subcutaneously transplanted P-388 leukemia revealed high selectivity of accumulation of the photosensitizer in tumors in comparison with normal tissues and high photodynamic activity of the preparation. This photosensitizer can be used as the basis for creating an effective preparation for photodynamic therapy and fluorescent diagnosis.
Subject(s)
Carcinoma, Ehrlich Tumor/drug therapy , Indoles/therapeutic use , Leukemia P388/drug therapy , Organometallic Compounds/therapeutic use , Photochemotherapy , Photosensitizing Agents/therapeutic use , Animals , Carcinoma, Ehrlich Tumor/diagnosis , Leukemia P388/diagnosis , Mice , Neoplasm TransplantationABSTRACT
The synthesis of new metallophthalocyanine-oligonucleotide conjugates is reported. These conjugates can cause sequence-specific photosensitized or catalytic oxidation of DNA by molecular oxygen.
Subject(s)
DNA/chemistry , Indoles/chemistry , Oligonucleotides/chemistry , Organometallic Compounds/chemistry , Radiation-Sensitizing Agents/chemistry , Catalysis , Indoles/chemical synthesis , Isoindoles , Oligonucleotides/chemical synthesis , Organometallic Compounds/chemical synthesis , Oxidation-Reduction , Photochemistry , Radiation-Sensitizing Agents/chemical synthesisABSTRACT
Comparative studies of oxygen consumption, changes of photosensitizer fluorescence, and photodestruction of erythrocytes, and photodestruction of oxygen transport protein hemoglobin were performed during photodynamic reaction in whole and hemolyzed blood with phthalocyanines, chlorines, porphyrins, and methylene blue photosensitizers in vitro and in selected cases in vivo. The present work deals with the investigation of blood oxygen saturation SO2 and photosensitizer fluorescence during and immediately after light irradiation in the photodynamic therapy process. It has been observed that SO2 behavior strongly correlates with the type of photosensitizer. The decrease of photosensitizer fluorescence (photobleaching) during light irradiation can be followed by the recovery of the photosensitizer fluorescence immediately after interruption of the irradiation within 6-8 min. The levels of photodestruction of erythrocytes in whole blood and photodestruction of hemoglobin in hemolyzed blood in combination with the above photosensitizers reveal the influence of photodynamic reactions upon the ability of blood to transport oxygen. Maximal photohemolysis activity has been found with chlorine p6 photosensitizers.
Subject(s)
Blood/radiation effects , Carcinoma, Ehrlich Tumor/blood , Heart Diseases/blood , Photochemotherapy , Photosensitizing Agents/administration & dosage , Absorption , Animals , Carcinoma, Ehrlich Tumor/drug therapy , Erythrocytes/drug effects , Erythrocytes/metabolism , Erythrocytes/radiation effects , Female , Fluorescence , Heart Diseases/drug therapy , Humans , Injections, Intravenous , Light , Mice , Mice, Inbred C57BL , Oxygen Consumption/radiation effects , Photosensitizing Agents/pharmacokineticsABSTRACT
The photodynamic effects of sulphonated zinc and aluminum phthalocyanine derivatives as well as phosphonated aluminum phthalOcyanine on the firing of isolated crayfish mechanoreceptor neurons were studied. After 30 min staining neurons were irradiated with He-Ne laser (632.8 nm, 0.3 W/cm2) and changes in neuron firing frequency were recorded. Neuron firing was found to be very sensitive to photodynamic effect and could serve as a sensitive indicator of cell photodamage. It changed the firing level and then died at nanomolar concentrations of phthalocyanines. The dynamics of the neuron responses to photodynamic effects included stages of firing activation and/or inhibition prior to irreversible firing abolition. The order of these stages depended on photosensitizer type and concentration. The comparison of the dependencies of neuron lifetime on photosensitizer concentrations showed ZnPcS2 to be the most effective photosensitizer.