Subject(s)
Aortic Aneurysm/diagnostic imaging , Aortic Dissection/diagnostic imaging , Echocardiography, Transesophageal , Tunica Intima/diagnostic imaging , Acute Disease , Aortic Dissection/surgery , Aortic Aneurysm/surgery , Aortic Valve/diagnostic imaging , Aortic Valve/surgery , Blood Vessel Prosthesis Implantation , Fatal Outcome , Humans , Iliac Artery/diagnostic imaging , Male , Middle Aged , Multiple Organ Failure/etiology , Pleural Effusion/diagnostic imaging , Postoperative ComplicationsABSTRACT
The aim of this study was to describe an high-performance liquid chromatographic assay for the simultaneous determination of two HIV protease inhibitors, saquinavir and ritonavir, in human serum. The method involved extraction of ritonavir and saquinavir from serum with the aid of solid-phase extraction C18 cartridges followed by high-performance liquid chromatography with a C8 column and ultraviolet detection set at a wavelength of 240 nm. The assay was linear and has been validated over the concentrations range of 0.5-32 microg/ml for ritonavir and 0.075-4.8 microg/ml for saquinavir, from 600 microl serum extracted. In future, the assay will be used to support human population pharmacokinetic studies, and therapeutic drug monitoring for ritonavir and saquinavir.