ABSTRACT
SnO particles were synthesized by an alkali-assisted hydrothermal and microwave methods. The aqueous-based reactions were carried out at pH ~ 8, under inert atmosphere (Ar). The reactions were taken under different times, and a full XRD structural analysis was made to evaluate the conversion from the Sn6O4(OH)4 intermediate to SnO particles. Williamson-Hall analysis showed that the size and strain of the SnO particles were time and route treatment dependent. Microwave heating yielded a single tetragonal SnO phase after 1 h of thermal treatment, and TEM images revealed spherical-shaped SnO nanoparticles with an average size of 9(1) nm. While by the hydrothermal treatment single SnO phase was obtained only after 4 hours, yielding non-uniform and elongated particles with sub-micrometric size. A dissolution-recrystallization process was taken into account as the mechanism for SnO particles formation, in which hydroxylated complexes, Sn2(OH)6-2, then condense to form the oxide. The time-shorting reaction provided by the microwave-assisted synthesis may be attributed to better heat distribution.
ABSTRACT
The biothrofic pathogen Albugo candida (Pers.) attacks all the cultivated Brassica species and several wild crucifers causing the disease known as white rust. The symptoms consist on the initial appearance of more or less conspicuous white sori on the plant leaves and stems, followed by severe distortions of the affected organs, especially flowers and siliqua. On delicate vegetable types of Brassica rapa L., like pak choi (B. rapa var. chinensis L.) and chinese cabbages (B. rapa var. pekinensis L.) even a slight white rust infection can render all the production unmarketable. Development of resistant cultivars is the best way to control the disease and to insure low pesticide spraying and residues in vegetables. The objective of this work were: i) the screening of a collection of B. rapa accessions from several seed banks for sources of resistance to white rust; and ii) the study of the inheritance of resistance in the most resistant accessions. Forty three accessions of B. rapa chinensis and 19 accessions of B. rapapekinensis were screened at the cotyledon stage following the methods of Santos and Dias (2004) with a Portuguese A. candida isolate Ac506 (Br) collected from turnip leaves. Four accessions of B. rapa chinensis presented more than 50% of resistant plants: the pak choi (BRA 117) was the most resistant accession with 85% of resistant plants, followed by pak choi (BRA227) with 73%, pai tsai 'Ex China 1' (B00083) with 60% and pak choi 'Ai Jiao Huang' (BRA 225) with 53%. On the opposite, B. rapa pekinensis accessions were all consider as susceptible presenting only 0-11% of resistant plants. The inheritance of resistance was studied in two crosses between pak choi BRA 117 and the rapid cycling B. rapa plant line CrGC 1.19. Selfed parents and F1 and F2 generations were produced, and screened at the cotyledon stage as previously described. Analysis of the results suggests that inheritance of resistance to Ac506 in pak choi BRA 117 is controlled by two nuclear genes with dominant recessive interaction.
Subject(s)
Brassica/physiology , Plant Diseases/microbiology , Brassica/genetics , Brassica/immunology , Brassica/microbiology , Brassica rapa/genetics , Brassica rapa/immunology , Brassica rapa/microbiology , Brassica rapa/physiology , China , Crosses, Genetic , Disease Susceptibility , Flowers/microbiology , Flowers/physiology , Immunity, Innate , Plant Diseases/immunology , Plant Leaves/microbiology , Plant Leaves/physiology , Seeds/physiologyABSTRACT
The mitochondrial import of 5-aminolevulinate synthase (ALAS), the first enzyme of the mammalian heme biosynthetic pathway, requires the N-terminal presequence. The 49 amino acid presequence transit peptide (psALAS) for murine erythroid ALAS was chemically synthesized, and circular dichroism and (1)H nuclear magnetic resonance (NMR) spectroscopies used to determine structural elements in trifluoroethanol/H(2)O solutions and micellar environments. A well defined amphipathic alpha-helix, spanning L22 to F33, was present in psALAS in 50% trifluoroethanol. Further, a short alpha-helix, defined by A5-L8, was also apparent in the 26 amino acid N-terminus peptide, when its structure was determined in sodium dodecyl sulfate. Heme inhibition of ALAS mitochondrial import has been reported to be mediated through cysteine residues in presequence heme regulatory motifs (HRMs). A UV/visible and (1)H NMR study of hemin and psALAS indicated that a heme-peptide interaction occurs and demonstrates, for the first time, that heme interacts with the HRMs of psALAS.
Subject(s)
5-Aminolevulinate Synthetase/chemistry , 5-Aminolevulinate Synthetase/metabolism , Heme/chemistry , Heme/metabolism , Amino Acid Sequence , Amino Acids/chemistry , Animals , Circular Dichroism , Magnetic Resonance Spectroscopy , Mice , Mitochondria/metabolism , Models, Molecular , Molecular Sequence Data , Peptides/chemistry , Protein Binding , Protein Structure, Secondary , Protein Structure, Tertiary , SpectrophotometryABSTRACT
OBJECTIVES: To assess the efficacy of desmopressin nasal spray compared with diclofenac given intramuscularly in patients with acute renal colic caused by urolithiasis. PATIENTS AND METHODS: The study included 61 patients randomized into three different groups; group A received desmopressin (40 microg, nasal spray), group B diclofenac (75 mg) intramuscularly and group C, both desmopressin and diclofenac. Pain was assessed using a visual analogue scale (a 10-cm horizontal scale ranging from 'no pain' to 'unbearable pain') at baseline, 10, 20 and 30 min after administering the treatments. RESULTS: On admission, the pain level was the same in all three groups. At 10 min the pain decreased in all groups to a level that was not significantly different. At 20 min groups B and C had similar mean pain levels (3.7), whereas in group A it was 5.0. At 30 min, groups B and C scored 2-3, and group A 5.6. All three treatments were equally effective at 10 and 20 min but at 30 min there was a stabilization/slight increase in pain level in group A. CONCLUSIONS: Desmopressin has several advantages, e.g. ease of administration, simplicity of delivery and apparent lack of side-effects, which makes it suitable for ambulatory use. Desmopressin acts rapidly and seems to be effective in both single and combined therapy with diclofenac; it decreases the need for a second treatment and increases the analgesic effect of diclofenac. Some patients responded well to desmopressin, with rapid and complete pain relief. These results indicate that desmopressin may be used to treat renal colic either alone or combined, increasing the analgesic effect of other drugs. More studies are needed to validate and confirm the results; it would also be useful to determine factors that may identify the subgroup of patients who respond quickly and with almost complete pain relief.
Subject(s)
Colic/drug therapy , Deamino Arginine Vasopressin/administration & dosage , Kidney Diseases/drug therapy , Renal Agents/administration & dosage , Administration, Intranasal , Anti-Inflammatory Agents, Non-Steroidal/administration & dosage , Diclofenac/administration & dosage , Drug Combinations , Female , Humans , Injections, Intramuscular , Male , Middle Aged , Pain Measurement , Time Factors , Treatment OutcomeABSTRACT
This is a descriptive study done in a qualitative perspective. It aims to describe the conceptions of health care units' managers about the work in public health. The subjects perceptions are that this work goes from interdisciplinary thought with a preventive focus to the curative practice of ambulatory care. On what concerns Nursing, nurses' management functions and auxiliaries assistance activities are pointed out. They believe that strategies to approximate to the SUS principles are necessary in the context of Public Health.
Subject(s)
Attitude of Health Personnel , Job Description , Models, Nursing , Nurse Administrators/organization & administration , Nurse Administrators/psychology , Nursing, Supervisory/organization & administration , Public Health Administration , Public Health Nursing/organization & administration , Adult , Brazil , Female , Humans , Male , Middle Aged , Nursing Assistants/organization & administration , Nursing Methodology Research , Nursing Staff/organization & administration , Patient Care Team/organization & administration , Surveys and QuestionnairesABSTRACT
Hemangioma is the most common benign neoplasm of the liver, for which a 'wait and see' policy has been advised when it is small or without symptoms. Surgery is the treatment of choice of these tumors when marked symptomatology is present. However, some of these lesions cannot be excised due to their size, multiplicity, location or medical conditions. In these patients, radiotherapy has been reported to give good results with minor morbidity, suspending the tumor growth and decreasing the symptomatology. Seven symptomatic patients were treated with radiotherapy with a dose of 15-30 Gray in 15-22 fractions. Improvement of the quality of life was observed in all patients and the regression of the tumor volume was confirmed by computed tomography in five, with a follow-up ranging from 40 to 67 months.