Fast and flexible solid-state linear transformer driver for plasma discharge based on metal oxide semiconductor field effect transistor.

A pulsed power supply with a short rise time and high repetition frequency is favorable to driving diffusive plasma for strongly oxidizing radical (O3, OH) generation and increasing the system's energy efficiency. In this paper, a 10-stage solid-state linear transformer driver (LTD) with a nanosecond rise time is developed to drive plasma for wastewater treatment. To decrease the rise time, a control system with low jitter is developed to improve the synchronization of pulses using an optocoupler isolation chip. A 10-stage LTD with a rise time of 6.2 ns is realized in the case that the rise time of the single-stage LTD is 5.4 ns. The results show that the LTD can generate pulses on a 300 Ω resistive load with a repetition frequency of 10 kHz, an amplitude of 8.80 kV, an overshoot less than 3.97%, and a reverse overshoot less than 4.82%. The rise time (6.2-33.0 ns), the pulse width (35.9-200.0 ns), and the fall time (10.5-27.6 ns) can be adjusted flexibly and independently by controlling the drive signals of metal oxide semiconductor field effect transistors. The pulsed generator is utilized to drive plasma in the needle-water electrode system. The preliminary experimental results show that the plasma includes abundant oxygen atoms and hydroxyl radicals with high activity, and it is suitable for wastewater treatment.

Efficacy and safety of bispecific antibodies vs. immune checkpoint blockade combination therapy in cancer: a real-world comparison.

Emerging tumor immunotherapy methods encompass bispecific antibodies (BSABs), immune checkpoint inhibitors (ICIs), and adoptive cell immunotherapy. BSABs belong to the antibody family that can specifically recognize two different antigens or epitopes on the same antigen. These antibodies demonstrate superior clinical efficacy than monoclonal antibodies, indicating their role as a promising tumor immunotherapy option. Immune checkpoints are also important in tumor immunotherapy. Programmed cell death protein-1 (PD-1) is a widely acknowledged immune checkpoint target with effective anti-tumor activity. PD-1 inhibitors have demonstrated notable therapeutic efficacy in treating hematological and solid tumors; however, more than 50% of patients undergoing this treatment exhibit a poor response. However, ICI-based combination therapies (ICI combination therapies) have been demonstrated to synergistically increase anti-tumor effects and immune response rates. In this review, we compare the clinical efficacy and side effects of BSABs and ICI combination therapies in real-world tumor immunotherapy, aiming to provide evidence-based approaches for clinical research and personalized tumor diagnosis and treatment.

A repetitive frequency square wave generator based on Blumlein pulse forming network and pseudospark switch.

The Blumlein pulse forming network (PFN) has widely been used in pulsed power technology to generate square waves with short pulse widths. In this paper, we developed a repetitive frequency square wave generator based on Blumlein PFN and pseudospark switch (PSS). A Blumlein PFN with unequal capacitances has been proposed, and the PFN parameters have been optimized for better output waveforms. A single-gap PSS with a withstand voltage of 40 kV and a repetitive frequency of 100 Hz has been designed to switch the Blumlein PFN. The experiment results show that the square wave generator can output pulses with a voltage of 26 kV, a rise time of 25 ns, and a pulse width of 90 ns on a matched load of 11 Ω. It has operated steadily for 10 h with a repetitive frequency of 100 Hz, and the jitter remains at around 1 ns after 1.05 × 106 shots.

A two-terminal fault location method for gas switch prefire in linear transformer driver.

Large-scale linear transformer drivers (LTDs) are composed of numerous high-power gas switches, and switch prefire is a frequent operational fault. To detect and locate the faulty switch accurately and efficiently, a two-terminal location method is proposed. A B-dot sensor is integrated on the gas switch's shell to collect the discharging signal. All the B-dot sensors are connected in parallel through cables of equal length. The fault position can be determined by the time delay of the signals at the two terminals. A diode is inserted between the B-dot sensor's coil and the cable core to ensure low-loss transmission of the signal. Two methods are applied in fault location, including time-of-arrival (TOA) and time reversal (TR). For the TOA method, an energy criterion and a phase criterion are applied and compared. The accuracy of the energy criterion is greatly influenced by the signal-to-noise ratio, while the phase criterion requires a reasonable estimate of the actual delay to account for the impact of phase periodicity. The TR method based on a precise simulation model is established, which demonstrates high precision in location. The TR method has been tested and validated on a single stage LTD module. Moreover, the location method for double switches prefire is discussed theoretically. The method proposed in this paper will be helpful to improve the efficiency of the commissioning, operation, and maintenance of the large-scale LTD devices.

The numerical simulation of a four-stage linear transformer driver module based on the Preisach magnetic core model.

The use of linear transformer drivers (LTDs) is widely considered the most promising technological approach for the development of future pulsed-power accelerators. In large-scale pulsed-power accelerators, abnormal conditions like switch prefire can occur frequently during tests and normal operations due to the presence of a large number of switches. The diagnosis of such faults based on signature waveforms requires further investigation. According to previous research, the characteristics of the magnetic cores greatly influence the fault waveforms. In this paper, a full-cycle mathematical model of the magnetic core is established utilizing a classical Preisach model based on experimental results. This model is coupled with the LTD circuit model in simulations, and simulation results are obtained under the condition of switch prefire. The simulation results are in good agreement with the experimental results from a four-stage LTD module with a sharing shell and de-ionized water insulated transmission line. The magnetization process of the cores is also determined under prefire conditions. Analyses of the magnetization process indicate that the completely demagnetized core shows high permeability under positive excitation and that the permeability abruptly decreases as the excitation is reversed. The hysteresis characteristics result in a phenomenon in which the output voltage in the prefired stage is almost unipolar. Finally, the features of the fault voltages captured in the experiments are also explained.

Microcystin-LR prenatal exposure drives preeclampsia-like changes in mice by inhibiting the expression of TGF-ß and VEGFA.

Microcystin-leucine-arginine (MC-LR) is widespread in the water and food, which has suspected to be associated with adverse pregnancy outcomes. In the present study, we aim to assess the interaction between MC-LR exposure and preeclampsia development and elucidate the molecular events involved. After exposure to MC-LR during pregnancy, the mice developed hypertension and proteinuria, the typical symptoms of preeclampsia. This was associated with decreased invasiveness of placental trophoblast and vascular dysplasia caused by MC-LR through down-regulating VEGFA and TGF-ß expression via AKT/m-TOR/HIF-1α pathway. In addition, this conclusion has been confirmed in a case-control study. Significantly, the addition of Deferoxamine (DFM), a phosphorylated serine-threonine protein kinases (p-AKT) specific agonist, can antagonize the inhibitory effect of MC-LR on the expression of related proteins, which further ameliorate the migration and invasion ability of HTR-8/Svneo cells. To sum up, our study revealed the pathologic mechanism by which MC-LR lead to preeclampsia and emphasized the importance of pregnancy management.

Transcriptomic responses of the liver of mandarin fish (Siniperca chuatsi) under hypoxic stress.

Environmental hypoxia is becoming more prevalent in aquatic environments of mandarin fish (Siniperca chuatsi) aquaculture because of eutrophication and climate change. Little information is available on the molecular mechanisms of the detrimental effects of hypoxia in this species. In this study, the authors assembled a transcriptome for mandarin fish exposed to lower oxygen conditions at different times (24 and 96 h). The antioxidant enzymatic activities of catalase, glutathione, superoxide dismutase, glutamic pyruvic transaminase and malondialdehyde significantly increased at 6 or 12 h but decreased after reaching a climax during 96 h hypoxia stress. The gene ontology study revealed 27,616 transcripts, whereas the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed 25 linked pathways. Significant changes in the expression of certain genes involved in protein processing in the endoplasmic reticulum, the calcium signalling system and inositol phosphate metabolism were discovered using the KEGG pathway analysis. In the liver, 97 genes were differentially expressed between the control and experimental groups. The expression level of 28 differentially expressed genes (DEGs) under different hypoxic stress conditions was detected using real-time PCR and compared to transcriptome sequencing results. The result showed that some genes in the experimental group associated with hypoxic stress, such as hif, ho-1a, ho-1b, igfbp1, hsp90α and hsp90 ß, were significantly upregulated compared with those in the control group. The large amount of transcriptome data from this research has enlarged the mandarin fish gene and genome bioinformation. The identified DEGs and pathways are useful in further studies of biological responses to hypoxia.

Handheld NIR-to-NIR Platform for on-site evaluating protective neutralizing antibody against SARS-CoV-2 ancestral strain and Omicron variant after vaccination or infection.

Lateral flow assays (LFAs) are promising points-of-care tests, playing a vital role in diseases screening, diagnosis and surveillance. However, development of portable, cheap, and smart LFAs platform for sensitive and accurate quantification of disease biomarkers in complex media is challenging. Here, a cheap handheld device was developed to realize on-site detection of disease biomarkers by Nd3+/Yb3+ co-doped near-infrared (NIR)-to-NIR downconversion nanoparticles (DCNPs) based LFA. Its sensitivity is at least 8-fold higher for detecting NIR light signal from Nd3+/Yb3+ co-doped nanoparticles than conventional expensive InGaAs camera based detection platform. Additionally, we enhance NIR quantum yield of Nd3+/Yb3+ co-doped nanoparticles up to 35.5% via simultaneous high dopant of sensitizer ions Nd3+ and emitter ions Yb3+. Combination of NIR-to-NIR handheld detection device and ultra-bright NIR emitting NaNbF4:Yb60%@NaLuF4 nanoparticle probe allows the detection sensitivity of SARS-CoV-2 ancestral strain and Omicron variants specific neutralizing antibodies LFA up to the level of commercial enzyme linked immunosorbent assay kit. Furthermore, by this robust method, enhanced neutralizing antibodies against SARS-CoV-2 ancestral strain and Omicron variants are observed in healthy participants with Ad5-nCoV booster on top of two doses of inactivated vaccine. This NIR-to-NIR handheld platform provides a promising strategy for on-site evaluating protective humoral immunity after SARS-CoV-2 vaccination or infection.

High voltage nanosecond pulse generator based on pseudospark switch and diode opening switch.

With the development of technology, low-temperature plasma plays an increasingly important role in industrial applications. The industrial application of low-temperature plasma has the following requirements for plasma, high electron energy, low macroscopic temperature, and uniformity. Low-temperature plasma driven by nanosecond pulses reflects more significant advantages in these aspects compared to direct current plasma and alternating current plasma. In this paper, a simple topology is proposed, which is based on the pseudospark switch and the diode opening switch. A pulse generator is developed, which can eventually output pulses with an amplitude of 106 kV, a rise time of 15.5 ns, a pulse width of 46 ns, and a maximum repetition rate of 1 kHz on a 260 Ω resistive load. The pulse generator can successfully drive needle-plate discharge plasma in ambient air. It has excellent parameters, stability, compactness, and a long lifetime. The proposed topology may be helpful for nanosecond pulse generators with amplitude ranging from tens to hundreds of kilovolts, which could be widely used in industry.

Salvianolic Acid B Regulates Oxidative Stress, Autophagy and Apoptosis against Cyclophosphamide-Induced Hepatic Injury in Nile Tilapia (Oreochromis niloticus).

Salvianolic acid B (Sal B), as one of the main water-soluble components of Salvia miltiorrhizae, has significant pharmacological activities, including antioxidant, free radical elimination and biofilm protection actions. However, the protective effect of Sal B on Nile tilapia and the underlying mechanism are rarely reported. Therefore, the aim of this study was to evaluate the effects of Sal B on antioxidant stress, apoptosis and autophagy in Nile tilapia liver. In this experiment, Nile tilapia were fed diets containing sal B (0.25, 0.50 and 0.75 g·kg-1) for 60 days, and then the oxidative hepatic injury of the tilapia was induced via intrapleural injection of 50 g·kg-1 cyclophosphamide (CTX) three times. After the final exposure to CTX, the Nile tilapia were weighed and blood and liver samples were collected for the detection of growth and biochemical indicators, pathological observations and TUNEL detection, as well as the determination of mRNA expression levels. The results showed that after the CTX treatment, the liver was severely damaged, the antioxidant capacity of the Nile tilapia was significantly decreased and the hepatocyte autophagy and apoptosis levels were significantly increased. Meanwhile, dietary Sal B can not only significantly improve the growth performance of tilapia and effectively reduce CTX-induced liver morphological lesions, but can also alleviate CTX-induced hepatocyte autophagy and apoptosis. In addition, Sal B also significantly regulated the expression of genes related to antioxidative stress, autophagy and apoptosis pathways. This suggested that the hepatoprotective effect of Sal B may be achieved through various pathways, including scavenging free radicals and inhibiting hepatocyte apoptosis and autophagy.

Alleviative effects of astragaloside IV on cyclophosphamide-induced oxidative damage and immunosuppression in tilapia (Oreochromis niloticus).

Astragaloside IV (ASIV) has effects of antioxidation and immunologic enhancement. However, there are few reports on the application and potential mechanism of ASIV in aquaculture. In this study, we investigated the effect of ASIV on growth, antioxidation, and immune function of tilapia. Tilapia were fed a diet containing 0.1, 0.2, and 0.5 g·kg-1 ASIV for 60 days, followed by an intrapleural injection of 50 mg·kg-1 cyclophosphamide (CTX) to induce oxidative damage and immunosuppression. Then tilapia were weighed and blood, liver, spleen, kidney, and intestinal were collected. The results showed ASIV increased the final weight, relative weight rate, and specific growth rate of tilapia, reduce conversion ratio, and reduced the morphological lesions of tissues. Meanwhile, ASIV alleviated CTX-induced oxidative damage by improving antioxidant activity in serum and tissues and inhibiting lipid peroxidation. Additionally, ASIV attenuated the immunosuppression of tilapia caused by CTX, regulated immunochemical indexes in serum, increased the viability of peripheral blood leukocytes and head kidney macrophages, and restored respiratory burst activity (O2-) in head kidney macrophages and splenocytes. Furthermore, qPCR data showed ASIV up-regulated antioxidant-related gene expression of nrf2, ho-1, gpx3, and cat and immune-related gene expression including C3 and igm. In conclusion, ASIV as a feed additive can not only improve the growth performance but also enhance the antioxidant capacity and immune function of tilapia, which may be associated with the ability of ASIV to scavenge free radicals, reduce lipid peroxidation levels, and stabilize numbers of immune cells.

Microcystin-LR inhibits early pregnancy by impairing the vascular network of luteum: Involvement of the MEK/ERK/SP1/VEGFR2 axis.

Microcystin-leucine-arginine (MC-LR) reduces the fertility of female mice, but the mechanism is unknown. We studied the effect of MC-LR on early pregnancy and elucidated its possible mechanism. The number of embryo beds and embryo volume decreased in pregnant mice at 6 or 8 days after fertilization after acute exposure to MC-LR. The corpus luteum secretes estrogen and progesterone, which are involved in embryo implantation and maintenance of early pregnancy. MC-LR exposure reduced luteal blood vessel branches and inhibited hormone synthesis. Functional blood vessels are essential to the maintenance of luteal structure and function. Reduced migration and tube-forming were also detected in human umbilical vascular endothelial cells (HUVECs) treated with MC-LR. MC-LR significantly decreased the expression of vascular endothelial growth factor receptor 2 (VEGFR2) in vivo and in vitro, which was responsible for the inhibited construction of the vascular network. The MEK/ERK/SP1 signal pathway mediated the decrease in VEGFR2 expression, and the agonists of phosphorylated extracellular regulated protein kinases (p-ERK) alleviated the anti-angiogenic effect of MC-LR. In conclusion, we demonstrated the toxicity of MC-LR on construction of vascular network in corpus luteum, which could provide a new perspective on female infertility or miscarriage caused by environmental factors.

The optical diagnosis of electrical wire explosion under a microsecond current pulse.

Electrical wire explosions have many applications in scientific research and industry. Optical diagnosis is a powerful method to clarify the evolutionary process of such explosions. In this paper, an experimental platform was established to diagnose the optical radiation of electrical wire explosions. A low-jitter trigatron switch and its trigger generator were designed to ensure accurate synchronization. The spatial-temporal evolution process and the self-emission spectrum of electrical explosion plasmas from different wires (copper and tantalum) were obtained and analyzed. The optical diagnosis results indicated that the electrical explosion of copper wire was mainly characterized by the inhomogeneity of partial ionization and the rapid expansion of the discharge channel. The spectrum in the early discharge stage of the copper wire electrical explosion was a continuum, and most of the self-radiation spectral lines belonged to Cu I or Cu II. At the later stage of the plasma dissipation process, the continuous spectrum gradually transformed into a line spectrum. The development of the tantalum wire discharge channel was relatively uniform, and the plasma was mainly established in the gas-liquid mixed phase channel of the tantalum wire. The self-emission spectrum of the tantalum wire was always continuous, and the absorption process of line spectrum radiation was distinct.

Single-cell phenotypic profiling to identify a set of immune cell protein biomarkers for relapsed and refractory diffuse large B cell lymphoma: A single-center study.

Diffuse large B-cell lymphoma (DLBCL) is the most common invasive type of non-Hodgkin lymphoma. Cell-of-origin (COO) classification is related to patients' prognoses. Primary drug resistance in treatment for DLBCL has been observed. The specific serum biomarkers in these patients who suffer from relapsed and refractory (R/R)-DLBCL remains unclear. In the current study, using single-cell RNA sequencing (scRNA-seq) and mass cytometry (CyTOF), we determined and verified immune cell biomarkers at the mRNA and protein levels in single-cell resolution from 18 diagnostic PBMC specimens collected from patients with R/R DLBCL. As controls, 5 PBMC specimens from healthy volunteers were obtained. We identified a panel of 35 surface marker genes for the features of R/R DLBCL unique cell cluster by scRNA-seq of 8 R/R DLBCL patient samples and validated its efficiency in an external cohort consisting of 10 R/R DLBCL patients by CyTOF. The cell clustering and dimension reduction were compared among R/R DLBCL samples in CyTOF Space with COO as well as the C-MYC expression designation. Immune cells from each patient occupied unique regions in the 32-dimensional phenotypic space with no apparent clustering of samples into discrete subtypes. Significant heterogeneity observed in subgroups was mainly attributed to individual differences among samples and not to expression differences in a single, homogeneous immune cell subpopulation. The marker panel showed reliability in labeling R/R DLBCL without any influence from COO stratification and C-MYC expression designation. Furthermore, we compared all the markers between R/R DLBCL and normal samples. A total of 12 biomarkers were significantly overexpressed in R/R DLBCL relative to the normal samples. Therefore, we further optimized the diagnostic biomarker panel of R/R DLBCL comprising CD82, CD55, CD36, CD63, CD59, IKZF1, CD69, CD163, CD14, CD226, CD84, and CD31. In summary, we developed a novel set of biomarkers for the diagnoses of patients with R/R DLBCL. Detections procedures at single-cell resolution provide precise biomarkers, which may substantially overcome intertumoral and intratumoral heterogeneity among primary samples. The findings confirmed that each case was unique and may comprise multiple, genetically distinct subclones.

Investigation on discharge process and switching characteristics of a pseudospark switch in series with a magnetic switch.

To reduce the commutation loss of the pseudospark switch, we connect a magnetic switch in series to the anode of the pseudospark switch. Herein, the typical discharge process and switching characteristics of a pseudospark switch in series with a magnetic switch are experimentally investigated at a different number of magnetic cores, gas pressures, and applied voltages. The results show that the addition of the magnetic switch extends the duration of the hollow cathode discharge of the pseudospark. Moreover, the rapid increase of the loop current and the fast decrease of the discharge voltage are separated, which is beneficial for reducing the commutation loss. With an increasing number of magnetic cores, the magnetic delay time increases and the commutation loss significantly decreases. However, the trigger delay time hardly changes since the magnetic switch does not affect the development of the pre-breakdown phase. At a fixed applied voltage, the trigger delay, magnetic delay time, and commutation loss decrease with increasing gas pressure. Furthermore, at a fixed gas pressure, the trigger delay of the pseudospark switch in series with a magnetic switch first decreases and then remains unchanged with increasing applied voltage, which is similar to that of a pseudospark switch without a magnetic switch because of the strong enough trigger injection. The magnetic delay time decreases and the commutation loss almost linearly increases with increasing applied voltage.

New advances in solid-state pulse generator based on magnetic switches.

Pulsed power technology is gradually forming a development trend of civil-military integration, which puts forward more requirements for pulsed power generators. This paper takes magnetic switches (MSs) as the starting point and reviews recent advancements in pulse generators based on MSs. First, the working mechanism of the MS "rapid inductance drop after magnetic core saturation" is analyzed. Second, the basic uses of MSs are introduced with specific examples, namely, magnetic compression unit, saturated pulse transformer, and magnetic delay switches. Then, the typical topologies of pulse generators based on MSs are discussed, including transmission line, Marx, Fitch, linear transformer driver, and semiconductor opening switch pumping circuits. These circuits' technical characteristics and parameter levels are highlighted. Finally, the existing problems and future development trends of MS-based solid-state pulse generators are discussed.

Self-triggering topology for high-power nanosecond pulse generators based on avalanche transistors Marx bank circuits and linear transformer driver.

In recent years, several novel avalanche transistor-based power synthesis topologies have been proposed to improve the output performance of pulse generators based on avalanche transistors. The most promising is the topology based on avalanche transistors Marx Bank Circuits (MBCs) and linear transformer driver (LTD). However, it suffers from the same problems as other semiconductor switch-based LTD generators. The greater the number of LTD modules, the higher the requirements for synchronization and drive capability of the trigger system. This paper proposes a new self-triggering topology for pulse generators based on avalanche transistors MBCs and LTD, which significantly simplifies the entire generator's requirement for trigger system synchronization and driving capability. First, the circuit topology and its operation principle are introduced. Then, three prototypes with one trigger LTD module and three self-triggering LTD modules are developed. The output characteristics are experimentally investigated. The results verify the feasibility of the proposed topology. Finally, the output amplitude and the rise time are 3.35 kV/3.7 ns, 4.12 kV/3.7 ns, and 4.88 kV/4.0 ns on a 25 Ω resistive load, respectively. All generators can operate at 1 kHz. The topology proposed in the article maximally simplifies the requirements for synchronization and drive capability of the trigger system for generators based on avalanche transistor MBCs and LTD.

Investigation of a novel ring-cusp magnetically confined plasma bridge neutralizer.

The plasma bridge neutralizer (PBN) based on a tungsten filament is a promising technique of a thermionic DC electron source where a hot filament is immersed in an inert gas flow and electrons are "bridged" from a small orifice to the ion beam. PBNs have been widely used in space propulsion and industrial applications due to their relatively simple structure and low power consumption. However, they have well-known disadvantages, namely, low emission current density and short lifetime. In this article, we propose a novel ring-cusp magnetically confined PBN (RCM-PBN) to address these issues. In the RCM-PBN, electrons are confined by a ring-cusp magnetic field, which improves the ionization efficiency and reduces the discharge chamber wall losses. Electrical insulation of the orifice plate from the chamber wall prevents a large number of electrons from being collected by the orifice plate, which greatly improves the extracted electron current. The effects of different operating parameters on the extracted electron current were studied through experiments. It was found that the increase in the extracted electron current with the extraction voltage was related to the anode spot formation. Analysis of the gas utilization factor and electron extraction cost shows that the optimal operating condition was obtained at an argon mass flow rate of 1.2 SCCM and a heater power of 45 W. At its optimum, a stable electron current of 1.1 A was extracted from the RCM-PBN with a gas utilization factor of 12.8 and an electron extraction cost of 143 W/A.

DesA Prognostic Risk Model of LncRNAs in Patients With Acute Myeloid Leukaemia Based on TCGA Data.

Purpose: This study aimed to combine the clinical data of acute myeloid leukaemia (AML) from The Cancer Genome Atlas (TCGA) database to obtain prognosis-related biomarkers, construct a prognostic risk model using long non-coding RNAs (lncRNAs) in AML and help patients with AML make clinical treatment decisions. Methods: We analysed the transcriptional group information of 151 patients with AML obtained from TCGA and extracted the expressions of lncRNAs. According to the mutation frequency, the patients were divided into the high mutation group (genomic unstable group, top 25% of mutation frequency) and low mutation group (genomic stable group, 25% after mutation frequency). The 'limma' R package was used to analyse the difference in lncRNA expressions between the two groups, and the "survival," "caret," and "glmnet" R packages were used to screen lncRNAs that are related to clinical prognosis. Subsequently, a prognosis-related risk model was constructed and verified through different methods. Results: According to the lncRNA expression data in TCGA, we found that seven lncRNAs (i.e. AL645608.6, LINC01436, AL645608.2, AC073534.2, LINC02593, AL512413.1, and AL645608.4) were highly correlated with the clinical prognosis of patients with AML, so we constructed a prognostic risk model of lncRNAs based on LINC01436, AC073534.2, and LINC02593. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses of differentially expressed lncRNA-related target genes were performed, receiver operating characteristic (ROC) curves were created, the applicability of the model in children was assessed using the TARGET database and the model was externally verified using the GEO database. Furthermore, different expression patterns of lncRNAs were validated in various AML cell lines derived from Homo sapiens. Conclusions: We have established a lncRNA prognostic model that can predict the survival of patients with AML. The Kaplan-Meier analysis showed that this model distinguished survival differences between patients with high- and low-risk status. The ROC analysis confirmed this finding and showed that the model had high prediction accuracy. The Kaplan-Meier analysis of the clinical subgroups showed that this model can predict prognosis independent of clinicopathological factors. Therefore, the proposed prognostic lncRNA risk model can be used as an independent biomarker of AML.

Dark/bright band of a melting layer detected by coherent Doppler lidar and micro rain radar.

Observation of a melting layer using a 1.55 µm coherent Doppler lidar (CDL) is first presented during a stratiform precipitation event. Simultaneous radar measurements are also performed by co-located 1.24 cm micro rain radar (MRR) and 10.6 cm Doppler weather radar (DWR). As a well-known bright band in radar reflectivity appears during precipitation, an interesting dark band about 160 m below that in lidar backscattering is observed. Due to the absorption effect, the backscattering from raindrops at 1.55 µm is found much weaker than that at short wavelengths usually used in direct detection lidars. However, the CDL provides additional Doppler information which is helpful for melting layer identification. For example, a spectrum bright band with broadened width and sign conversion of skewness is detected in this case. After a deep analysis of the power spectra, the aerosol and precipitation components are separated. The fall speed of hydrometeors given by CDL is found smaller than that of MRR, with the differences of approximately 0.5 m/s and 1.5 m/s for the snow and rainfall, respectively. To illustrate the influence of absorption effect, simulations of the backscatter coefficient and extinction coefficient of aerosol and rainfall are also performed at the wavelength range of 0.3 ∼ 2.2 µm using the Mie theory.