Cross-Talk and Multiple Control of Target of Rapamycin (TOR) in Sclerotinia sclerotiorum.

Sclerotinia sclerotiorum is a necrotrophic phytopathogenic fungus that cross-talks with its hosts for control of cell-death pathways for colonization. Target of rapamycin (TOR) is a central regulator that controls cell growth, intracellular metabolism, and stress responses in a variety of eukaryotes, but little is known about TOR signaling in S. sclerotiorum. In this study, we identified a conserved TOR signaling pathway and characterized SsTOR as a critical component of this pathway. Hyphal growth of S. sclerotiorum was retarded by silencing SsTOR, moreover, sclerotia and compound appressoria formation were severely disrupted. Notably, pathogenicity assays of strains shows that the virulence of the SsTOR-silenced strains were dramatically decreased. SsTOR was determined to participate in cell wall integrity (CWI) by regulating the phosphorylation level of SsSmk3, a core MAP kinase in the CWI pathway. Importantly, the inactivation of SsTOR induced autophagy in S. sclerotiorum potentially through SsAtg1 and SsAtg13. Taken together, our results suggest that SsTOR is a global regulator controlling cell growth, stress responses, cell wall integrity, autophagy, and virulence of S. sclerotiorum. IMPORTANCE TOR is a conserved protein kinase that regulates cell growth and metabolism in response to growth factors and nutrient abundance. Here, we used gene silencing to characterize SsTOR, which is a critical component of TOR signaling pathway. SsTOR-silenced strains have limited mycelium growth, and the virulence of the SsTOR-silenced strains was decreased. Phosphorylation analysis indicated that SsTOR influenced CWI by regulating the phosphorylation level of SsSmk3. Autophagy is essential to preserve cellular homeostasis in response to cellular and environmental stresses. Inactivation of SsTOR induced autophagy in S. sclerotiorum potentially through SsAtg1 and SsAtg13. These findings further indicated that SsTOR is a global regulator of the growth, development, and pathogenicity of S. sclerotiorum in multiple ways.

High-contrast ratio and high-efficiency anamorphic DLP illumination system design with a freeform surface lens.

In this paper, an anamorphic digital light processing illumination system design with a freeform surface lens is described. A lens with a freeform surface lens is combined with the achromatic total internal reflection prism set for application in a relay lens system. In order to achieve a high contrast ratio without sacrificing illumination system efficiency, the novel, to the best of our knowledge, design uses an x-y polynomial surface for the freeform surface lens to reduce the overlap angle of the anamorphic angular distribution in angular space. Moreover, there is no need to use a baffle at the aperture stop in this novel design. We can reach a half-cone angle of 17.49° in the y direction of the angular distribution in angular space. The overlap angle is only 0.98°. Finally, with this novel design, we can achieve a high contrast ratio of 9128:1 and an illumination system efficiency is 66.4%. The four-corner average spot size at the active area of the digital micromirror device chip is controlled at about 240 µm, and the optical distortion is less than 1%. The corresponding lateral color aberration is 3.61 µm.