Development of a wound epithelialization healing model: reducing the impact of contraction healing on the wound surface.

Animal experiments are important in trauma-related studies because they simulate in vivo effects. Rodents are a good choice for preparing trauma models; however, contractile healing in rodents results in a healing pattern that differs considerably from that in humans. Therefore, this study developed a new rodent model that avoids contractile healing of the skin around the wound using an anti-contraction ring, and the skin in the wound's center remains intact and acts as a source for epithelialized diffusion healing. Cell proliferation, migration, revascularization, and collagen secretion did not differ between the novel and conventional full-skin defect trauma models. However, the healing rate at various stages significantly differed between the two groups owing to differences in the healing patterns. And without effective treatment, the experimental group cannot heal. The stabilities of the novel and conventional methods were good regardless of operator or batch. In summary, this new animal trauma model provides a stable experimental environment similar to that in humans, which may promote trauma-related research.

Platelet-Rich Fibrin Accelerates Skin Wound Healing in Diabetic Mice.

Diabetic foot ulcers (DFUs) are associated with an increased risk of secondary infection and amputation. Platelet-rich fibrin (PRF), a platelet and leukocyte concentrate containing several cytokines and growth factors, is known to promote wound healing. However, the effect of PRF on diabetic wound healing has not been adequately investigated. The aim of the study was to investigate the effect of PRF on skin wound healing in a diabetic mouse model. Platelet-rich fibrin was prepared from whole blood of 8 healthy volunteers. Two symmetrical skin wounds per mouse were created on the back of 16 diabetic nude mice. One of the 2 wounds in each mouse was treated with routine dressings (control), whereas the other wound was treated with PRF in addition to routine dressings (test), each for a period of 14 days. Skin wound healing rate was calculated.Use of PRF was associated with significantly improved skin wound healing in diabetic mice. On hematoxylin and eosin and CD31 staining, a significant increase in the number of capillaries and CD31-positive cells was observed, suggesting that PRF may have promoted blood vessel formation in the skin wound. In this study, PRF seemed to accelerate skin wound healing in diabetic mouse models, probably via increased blood vessel formation.

Reduction mammaplasty with the modified round block technique in Chinese patients and its anatomy study.

BACKGROUND: Although reduction mammaplasty is one of the most common procedures in plastic surgery, it is associated with some serious complications, especially in cases of severe hypertrophy or advanced ptosis, including necrosis of the nipple and areola, absence of normal sensation of the nipple-areolar complex, and loss of lactational function for future nursing. A thorough understanding of the breast anatomy is vital for successful reduction mammaplasty. This article describes the blood supply and innervation of the breasts, and introduces a modified round block technique for reduction mammaplasty based on this anatomy. METHODS: Six adult female cadavers were used to assess the vasculature of the anterior thorax and the nerve distribution of the breasts according to vascular perfusion and autopsy. Based on this anatomical study, a modified round block technique was designed for reduction mammaplasty, which was performed in 40 Chinese patients with breast hypertrophy. RESULTS: Würinger's horizontal septum was observed in all six cadavers. In the superficial layer, the main sources of blood are the second and third intercostal perforating branches of the internal thoracic artery, which run along the medial ligaments, and the branches of the lateral thoracic artery, which run along the lateral ligaments. In the deep layer, the main sources of blood are the fourth and fifth intercostal artery perforators, which run along Würinger's horizontal septum. Innervation of the nipple-areolar complex is achieved mainly by the lateral branches of the fourth intercostal nerve, which also run along Würinger's horizontal septum toward the nipple-areolar complex. The 40 patients with breast hypertrophy underwent successful reduction mammaplasty using our modified round block technique, except for one case, which developed necrosis of the nipple-areolar complex. CONCLUSIONS: Reduction mammaplasty using our modified round block technique can maximally preserve the blood supply to the remaining gland as well as the innervation to the nipple-areolar complex, while maintaining the advantages of the traditional technique, such as an invisible scar and good projection.