Amino acids inhibit the photodynamic inactivation effect by hindering cellular oxidative stress.

Photodynamic inactivation (PDI) could utilize light to activate reactive oxygen species (ROS) produced by photosensitizers to kill bacteria for preservation. To delve into the complex effects arising during the post-harvest PDI processing, we conducted experiments using Pseudomonas reinekei, a food spoilage bacteria extracted from rotten Pakchoi. Through analyzing the metabolomics results, we discovered that methionine (Met) and glutamate (Glu) exhibited significant inhibitory effects during the PDI process. The oxidative stress generated by light treatment resulted in a reduction of 30.31% and 36.37% in the levels of Met and Glu, respectively. The data also showed that exogenous Met and Glu reduced intracellular oxidative stress levels, increased peroxidase activity, and prevented the damage of intracellular material and cell membrane deformation. That amino acids could inhibit the effect of PDI by hindering oxidative stress. Therefore, the amino acid content should be considered when applying PDI to treat Met- or Glu-rich foods.

Recognition and detection of histamine in foods using aptamer modified fluorescence polymer dots sensors.

Histamine has been known as a momentous cause of biogenic amine poisoning. Therefore, the content of histamine in foods is strictly required to be controlled within a certain range. Here, an aptamer fluorescent sensor was developed for detection of histamine. Poly [(9, 9-di-n-octylfluorenyl-2, 7-diyl)-alt-(benzo [2,1,3] thiadia-zol-4, 8-diyl)] (PF8BT) and the styrene maleic anhydride copolymer (PSMA) were used for the preparation of PF8BT-Polymer dots (PF8BT-Pdots). PF8BT-Pdots and the cyanine3-phosphoramidite (Cy3) were linked through aptamer to achieve the ratiometric detection for histamine. PF8BT-Pdots were partly quenched by Cy3 due to the fluorescence resonance energy transfer (FRET), when the histamine molecule was recognized by aptamer on the surface of PF8BT-Pdots. A linear range (3-21 µmol/L) was obtained for histamine detection with a low limit of detection (LOD = 0.38 µmol/L). PF8BT aptamer Pdots (PF8BT-A) were used to detect histamine in simply treated aquaculture water and tuna. The cell imaging of HeLa cells presented a good biosecurity and outstanding fluorescent imaging capability of PF8BT-A. The aptamer fluorescent sensors provided a new platform for rapid and accurate detection of histamine in aquatic products and had great potential for the application in food safety and quality control.

Alizarin complexone modified UiO-66-NH2 as dual-mode colorimetric and fluorescence pH sensor for monitoring perishable food freshness.

Food prone to spoilage has a huge food safety hazard, threatening people's health, so early detection of food spoilage is a continuous and urgent need. Herein, we developed a dual-mode response sensor, alizarin complexone@UiO-66-NH2, which can accurately detect pH. The sensor demonstrated significant changes in color from pale yellow to deep pink, while the fluorescence shifted from light blue to blue violet. Moreover, both UV absorption and fluorescence intensity showed a linear correlation with pH raging from 4.5 to 7.5. These results indicate that the sensor effectively responds to pH, making it suitable for detecting the freshness of perishable food. To put this into practice, we integrated the sensor with cellulose-based filter paper to determine the freshness of shrimp and beef, which was proved to be effective in assessing freshness. In the future, it can be combined with intelligent colorimetric and fluorescence instruments to achieve visual detection.

Oral immunization with recombinant L. lactis expressing GCRV-II VP4 produces protection against grass carp reovirus infection.

The hemorrhagic disease causing by grass carp reovirus (GCRV) infection, is associated with major economic losses and significant impact on aquaculture worldwide. VP4 of GCRV is one of the major outer capsid proteins which can induce an immune response in the host. In this study, pNZ8148-VP4/L. lactis was constructed to express recombinant VP4 protein of GCRV, which was confirmed by the Western-Blot and enzyme-linked immunosorbent assay. Then we performed the oral immunization for rare minnow model and the challenge with GCRV-II. After oral administration, pNZ8148-VP4/L. lactis can continuously reside in the intestinal tract to achieve antigen presentation. The intestinal and spleen samples were collected at different time intervals after immunization, and the expression of immune-related genes was detected by real-time fluorescence quantitative PCR. The results showed that VP4 recombinant L. lactis could induce complete cellular and humoral immune responses in the intestinal mucosal system, and effectively regulate the immunological effect of the spleen. The immunogenicity and the protective efficacy of the oral vaccine was evaluated by determining IgM levels and viral challenge to vaccinated fish, a significant level (P < 0.01) of antigen-specific IgM with GCRV-II neutralizing activity was able to be detected, which provided a effective protection in the challenge experiment. These results indicated that an oral probiotic vaccine with VP4 expression can provide effective protection for grass carp against GCRV-II challenge, suggesting a promising vaccine strategy for fish.

Approaching Effective Differential Centrifugal Fractionation by Combining Image Analysis with Analytical Ultracentrifugation.

Centrifugation is one of the most commonly used methods for separation in biology and chemistry. However, effective fractionation is not always easy to obtain, as preparative centrifuge experiments are mostly conducted in an empirical way, even when it is guided by the quantitative results from analytical ultracentrifuge (AUC). Very few works have been performed to enhance the fractionation resolution of the differential centrifugation method in a swing-out rotor. This is primarily due to the absence of a characterization tool for sedimentation in the preparative centrifuge. In this study, we utilized image analysis to map the particle concentration distribution throughout the preparative centrifuge tube, revealing an unexpected and abnormal sedimentation process. By characterizing the sedimentation coefficient distributions of the fractionated product via AUC, we demonstrated that the overall sedimentation efficiency in a swing-out preparative centrifuge was significantly reduced. Furthermore, effective fractionation was confined to the intermediate phase of the entire sedimentation process. We propose that the mechanism here is a combination of the inverse Boycott effect and droplet sedimentation. The actual sedimentation process within a preparative centrifuge can be described by modifying the Lamm equation phenomenologically, which simply results in an effective sedimentation coefficient. Our work builds a foundation for determining the optimal preparative centrifugation conditions for various systems.

Development of packaging films based on UiO-66 MOF loaded melatonin with antioxidation functions for spinach preservation.

Spinach tends to deteriorate after harvest due to physiological metabolic activities. As a natural, pollution-free, and environmentally friendly preservative, melatonin (MT) can effectively maintain the quality of fruits and vegetables after harvest and delay senescence. To enhance the preservation effect of MT, this study developed antioxidant films using MT-loaded UiO-66 metal-organic framework (MOF) nanoparticles. This approach effectively extends the shelf life of spinach while preserving its quality. The underlying mechanism involves leveraging the microporous structure and stability of UiO-66 MOF. Experimental results obtained from the packaging films demonstrated significant improvements in both mechanical strength and antioxidant properties when UiO-66 was loaded with MT at a concentration of 0.20 mg/mL and combined with sodium alginate. Freshness preservation experiments also indicated the effective preservation effect of these films on spinach. In conclusion, the results of this study suggest that MT-loaded UiO-66 MOF is a promising active packaging material for spinach preservation.

The application of ice glazing containing D-sodium erythorbate combined with vacuum packaging to maintain the physicochemical quality and sweet/umami non-volatile flavor compounds of frozen stored large yellow croaker (Pseudosciaena crocea).

Ice glazing containing 0.3 % D-sodium erythorbate (DSE), combined with vacuum packaging, was used as a method to maintain the quality of large yellow croaker during frozen storage. This study aimed to assess various aspects, including water properties (water holding capacity and moisture distribution), protein-related characteristics (secondary and tertiary structure of myofibrillar protein), freshness indicators (K value and total volatile base nitrogen (TVB-N)), and non-volatile flavor compounds (free amino acids (FAAs) and nucleotides) in samples stored for 300 days at -23 °C. The results showed that vacuum packaging had a significant inhibitory effect on the growth of ice crystal. Notably, it successfully maintained the cross-sectional area of nearly all ice crystals below 20,000 µm2, effectively curtailing water loss. Simultaneously, the combination of vacuum packaging with the complex ice glaze effectively mitigated the degradation of IMP and free amino acids, maintaining low levels of K value (12.85 %) and TVB-N (11.28 mg N/100 g) throughout the 300-day frozen storage, retaining first-class freshness. Among the various treatment modalities assessed, the combined application of vacuum packaging and 0.3 % DSE-infused ice glazing emerged as the most effective in terms of preservation outcomes. This efficacious combination shows promising potential for the frozen storage of aquatic products and is therefore recommended for practical implementation.

Recent Study of Separation and Identification of Micro- and Nanoplastics for Aquatic Products.

Micro- and nanoplastics (MNPs) are polymeric compounds widely used in industry and daily life. Although contamination of aquatic products with MNPs exists, most current research on MNPs focuses on environmental, ecological, and toxicological studies, with less on food safety. Currently, the extent to which aquatic products are affected depends primarily on the physical and chemical properties of the consumed MNPs and the content of MNPs. This review presents new findings on the occurrence of MNPs in aquatic products in light of their properties, carrier effects, chemical effects, seasonality, spatiality, and differences in their location within organisms. The latest studies have been summarized for separation and identification of MNPs for aquatic products as well as their physical and chemical properties in aquatic products using fish, bivalves, and crustaceans as models from a food safety perspective. Also, the shortcomings of safety studies are reviewed, and guidance is provided for future research directions. Finally, gaps in current knowledge on MNPs are also emphasized.

Prolong the postharvest shelf life of spinach through the antioxidative ability of melatonin.

Spinach is also known as Persian cuisine, it is rich in nutrients such as protein, vitamin C and minerals, and has high nutritional value. In this study, Spinach was treated with melatonin in order to prolong its shelf life. Melatonin has strong antioxidant effects as an endogenous free radical scavenger. The spinach was sprayed with 0.10, 0.20 and 0.30 mg/mL melatonin solution after harvesting, and distilled water was used as control for low temperature storage at 4 °C. The results showed that melatonin spraying Spinach delayed the degradation of chlorophyll, especially the treatment of 0.20 mg/mL melatonin was the most effective. The content of soluble sugar and soluble protein in spinach tissue was kept high, the accumulation of malondialdehyde (MDA) was reduced, and the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were increased. These findings suggested that melatonin treatment may be a useful technique to prolong the postharvest life of spinach and improve its quality.

Corrigendum to "Prolong the postharvest shelf life of spinach through the antioxidative ability of melatonin" [Food Chem. 19 (2023) 100769].

[This corrects the article DOI: 10.1016/j.fochx.2023.100769.].

Factors affecting the quality of frozen large yellow croaker (Pseudosciaena crocea) in cold chain logistics: Retention time and temperature fluctuation.

The purpose of this study is to provide a reference for avoiding the quality loss of large yellow croaker in cold chain transportation. The effects of retention time before freezing and temperature fluctuation caused by transshipment in logistics were evaluated by TVB-N, K value, TMA value, BAs, FAAs content and protein-related characteristics. The results showed that the retention would lead to the rapid increase of TVB-N, K value, and TMA value. And the temperature fluctuation would further lead to deterioration of these indicators. We concluded that the influence of retention time was far greater than that of temperature fluctuation. In addition, the bitter free amino acids (FAAs) were highly correlated with the freshness-related indicators, which could reflect the freshness changes of samples, especially the quantity of histidine. Therefore, it is suggested to freeze samples immediately after catching and try to avoid temperature fluctuations during cold chain to maintain the quality.

Apoptotic Changes, Oxidative Stress and Immunomodulatory Effects in the Liver of Japanese Seabass (Lateolabrax japonicus) Induced by Ammonia-Nitrogen Stress during Keep-Live Transport.

This study investigated the effects of NH3-N on antioxidant responses, histoarchitecture, and immunity of Japanese seabass (Lateolabrax japonicus) during keep-live transport. The findings suggest that NH3-N stress transport alters the transcription of P53, Caspase 9, Bcl2, Caspase 3 and Bax genes, demonstrating that NH3-N stress can trigger the apoptotic pathway of P53-Bax-Bcl2 and Caspase and induce apoptosis. NH3-N stress transport also evoked transcriptional upregulation of inflammatory cytokines (tumor necrosis factor α (TNF-α), Toll-like receptor 3 (TLR-3), nuclear factor kappa ß (NF-κB), interleukin 6 (IL-6) and interleukin 1ß (IL-1ß)) and increased complement C3, C4, lysozyme (LZM) and immunoglobulin (IgM) levels, activating the innate immunological system during keep-live transport. In addition, NH3-N stress transport altered changes in the levels of superoxide dismutase (SOD), catalase (CAT), glutathione-related enzymes, and heat shock proteins 70 and 90 in the liver, indicating that the antioxidant system and Hsp protected the cells from NH3-N-induced oxidative stress. When excess ROS were not removed, they caused the body to respond with immunological and inflammatory responses, as well as apoptosis and tissue damage. This helps towards understanding the effect of NH3-N levels on sea bass during keep-live transport.

Nanoscale semiconducting polymer dots with rhodamine spirolactam as fluorescent sensor for mercury ions in living systems.

Mercury ion (Hg2+), as one of the most poisonous heavy metal ions, could seriously damage mental and neurological functions thus causing severe diseases. A fluorescent ratiometric sensor based on semiconducting polymer dots (Pdots) and rhodamine spirolactam derivate was developed for the detection of Hg2+. The Pdots were prepared by Poly [(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-diphenylene-vinylene-2-methoxy-5-{2-ethylhexyloxy}-benzene)] (PDDB) with emitting strong green fluorescence. The organic fluorescence dye N-(rhodamine-B) lactam-hydrazine (RhBH), as Hg2+-recognizing monomer, was conjugated to the surface of Pdots. Hg2+ could specifically trigger ring-opening process of RhBH and thus induce strong Förster resonance energy transfer (FRET) effect, resulting in the green fluorescence decrease of Pdots (energy donor) and red emission derived from the ring-opened RhBH (energy acceptor) increasing. PDDB@RhBH showed a sensitive and reversible response toward Hg2+ and had a great performance on resisting interferences from various biological analytes. Additionally, both fluorescent imaging in living cells and zebrafish, and systemic toxicity analysis in rats demonstrated that PDDB@RhBH was a great potential fluorescent sensor for quantitative Hg2+ imaging in living systems.

Potential of Good's buffers to inhibit denaturation of myofibrillar protein upon freezing.

The current systematic study sought to examine the potential use of three Good's buffers (MES, MOPS and HEPES) in inhibiting myofibrillar protein (MFP) denaturation induced by acidity changes. The highest degree of acidity variation was found in the center and bottom of large bottles due to the freeze-concentration effect. Good's buffer tended to basify during freezing, and it could prevent the crystallization of sodium phosphate (Na-P) buffer. Acidification upon freezing Na-P disrupted the natural conformation of MFP and induced the formation of large proteins aggregates with tight packing. The 15 mM MES, 20 mM MOPS, and 30 mM HEPES were respectively added to neutralize the strong acidity drop induced by freezing 20 mM Na-P, and all of them significantly improved the stability of the MFP conformation (P < 0.05). This work is not only critical to meet the growing demand for protein, but also groundbreaking for broadening the applicability of Good's buffers in the food industry.

Construction of a nanoscale metal-organic framework aptasensor for fluorescence ratiometric sensing of AFB1 in real samples.

Aflatoxins B1 (AFB1) that could contaminate agricultural products has received sustained attention due to its high toxicity and wide distribution. Therefore, sensitive and facile detection method for AFB1 is significant for food safety and control. In this work, a ratiometric fluorescence NMOFs-Aptasensor was developed based on the combination of Cy3-modified aptamer and zirconium-based nanoscale metal-organic frameworks (NMOFs). NMOFs served as energy donors, and Cy3 labeled on the AFB1 aptamer was used as an acceptor. An energy donor-acceptor pair was fabricated in the NMOFs-Aptasensor. With AFB1 selectively caught by the AFB1 aptamer, the fluorescence of the NMOFs-Aptasensor changed via fluorescence resonance energy transfer (FRET), and the fluorescence spectra changed accordingly. The ratiometric fluorescence signal was utilized to quantitatively measure AFB1. The reported NMOFs-Aptasensor presented great detection performance from 0 to 3.33 ng mL-1, with an LOD of 0.08 ng mL-1. Moreover, the fluorescence sensor was successfully applied to detect AFB1 in real samples.

Evaluation on the effect of ice glazing with different compound additives on the quality of frozen stored (-23 °C) large yellow croaker (Pseudosciaena crocea).

BACKGROUND: Compounded ice glazing has been used in large yellow croaker to improve its quality during frozen storage. The ice glazing liquid is prepared by compound use of trehalose and tea polyphenols, and the moisture, protein-related properties and freshness of the fish have been evaluated during 300 days of frozen storage. RESULTS: The results showed that the addition of trehalose effectively reduced the loss of water. At the same time, it was difficult for ice crystals to grow under the action of trehalose, the average diameter could still be maintained at 111.25-119.85 µm. The combination with tea polyphenols could effectively maintain the protein structure and keep the total volatile base nitrogen (TVB-N) and K value within 11.84 mg/100 g and 13.18%, so that the freshness of the fish was always at the first level. CONCLUSION: In a word, the ice glazing with 5% trehalose and 8% tea polyphenols had the best preservation effect, which was recommended for the frozen storage. © 2022 Society of Chemical Industry.

Aptamers-functionalized nanoscale MOFs for saxitoxin and tetrodotoxin sensing in sea foods through FRET.

Saxitoxin (STX) and tetrodotoxin (TTX) are widely distributed and extremely harmful marine toxins, it is certainly worth to spend effort to develop facile methods to detect them in sea food for human safety. In this work, two nano-sensors were developed by combining with two zirconium fluorescence Nanoscale metal-organic frameworks (NMOFs) with two emissions and TAMRA-labelled aptamers for STX and TTX sensing, respectively. The recognition of STX and TTX by these nano-sensors could change the structure of aptamer, which caused the blue or green emissions from NMOFs (energy donor) decreased while red emission from TAMRA-labelled aptamers (energy acceptor) increased owing to fluorescence resonance energy transfer (FRET) effect. Based on this strategy, NMOFs-Aptasensor 1 and NMOFs-Aptasensor 2 were developed for the ratiometric detection, with detection limits of 1.17 nM and 3.07 nM for STX and TTX, respectively. Moreover, NMOFs-Aptasensors displayed significant stability, pH-independence, selectivity and NMOFs-Aptasensors were successfully applied in shellfish sample for toxin sensing.

Chlorophyllin-Based 405 nm Light Photodynamic Improved Fresh-Cut Pakchoi Quality at Postharvest and Inhibited the Formation of Biofilm.

The aim of this study was to evaluate the effect of chlorophyllin-based photodynamic inactivation (Chl-PDI) on biofilm formation and fresh-cut pakchoi quality during storage. Firstly, Chl-based PDI reduced the amount of biofilm in an in vivo experiment and inactivated the food spoilage bacteria. Antibacterial mechanism analysis indicated that the bacterial extracellular polysaccharides and extracellular proteins were vulnerable targets for attacks by the Chl-based PDI. Then, the food spoilage microorganisms (Pseudomonas reinekei and Pseudomonas palleroniana) were inoculated onto the surface of fresh-cut pakchoi. We used chlorophyllin (1 × 10-5 mol/L) and 405 nm light (22.27 J/cm2 per day) to investigate the effect of Chl-based PDI treatment on fresh-cut pakchoi quality during storage. The results showed that Chl-based PDI increased the visual quality and the content of chlorophyll, VC, total soluble solids, and SOD activity and decreased the occurrence of leaf yellowing and POD activity. These suggest that Chl-based PDI can be used for the preservation of fresh-cut pakchoi and has the potential to inhibit biofilm formation of food spoilage bacteria. It is of great significance for the effective processing and traditional vegetable preservation.

Construction of Effective Nanosensor by Combining Semiconducting Polymer Dots with Diphenylcarbazide for Specific Recognition of Trace Cr (VI) Ion in Water and Vitro.

Hexavalent chromium (Cr (VI)) ion, as highly toxic environmental pollution, severely endangers the ecological environment and public health. Herein, a fluorescent nanosensor (PFO-DPC) was constructed by combining semiconducting polymer dots with diphenylcarbazide (DPC) for sensing Cr (VI) ion in aqueous solution and living cells. DPC and poly (styrene-co-maleic anhydride) (PSMA) polymer mixed with polyfluorene (PFO) were utilized for selectively indicating Cr (VI) ion and improving the efficiency of detection, respectively. The presence of Cr (VI) ion effectively turned off the blue and green fluorescence of PFO-DPC in the aqueous environment, and the fluorescence quenching efficiency exhibited a good linear relationship between the range of 0.0 to 2.31 nM (R2 = 0.983) with a limit of detection (LOD) of 0.16 nM. The mechanism of fluorescence quenching could possibly be attributed to the internal filtration effect (IFE). Additionally, PFO-DPC showed a satisfactory performance in monitoring intracellular Cr (VI) ion. Our results indicate that the sensor is promising in various applications.

The HD-GYP domain protein of Shewanella putrefaciens YZ08 regulates biofilm formation and spoilage activities.

Shewanella putrefaciens is an important spoilage bacteria in seafood and its ability to form biofilms in food processing environments increases the chances of food spoilage. Exploring the regulatory factors associated with biofilm formation and spoilage activity in S. putrefaciens is of great significance for extending the shelf life of seafood. In this work, the regulatory function of HD-GYP domain protein K2227_17660 in spoilage microorganism S. putrefaciens YZ08 was studied. The deletion mutant Δ17660 was developed to explore the effects of K2227_17660 in c-di-GMP content regulation, motility, biofilm formation, extracellular protease activity, and spoilage potential by phenotypic and transcriptional comparison with wild-type (WT) strain. Deletion of K2227_17660 significantly increased c-di-GMP content, biofilm biomass, the production of extracellular polysaccharide, trimethylamine (TMA), and putrescine compared with WT strains, and also affected membrane fatty acid composition. Furthermore, RT-qPCR results revealed the expression levels of genes associated with biofilm biomass, spoilage and unsaturated fatty acids (UFAs) synthesis changed in a manner consistent with the phenotypes. Our results indicated that K2227_17660 possesses phosphodiesterase (PDE) activity that controls the biofilm biomass and spoilage potential of S. putrefaciens. This study provided a basis for a correlation between c-di-GMP and food spoilage in S. putrefaciens, providing new insights into the control of food quality and safety.