Quercetin in semen extender improves frozen-thawed spermatozoa quality and in-vivo fertility in crossbred Kamori goats.

This study investigated the antioxidant effect of quercetin-treated semen on frozen-thawed spermatozoa quality and in-vivo fertility in crossbred Kamori goats. In total, 32 ejaculates from four fertile bucks were diluted in Tris-based egg yolk extender with varying levels of quercetin (0, 1, 5, 10, and 15 µM). Qualified semen samples were pooled and frozen in French straws. The results revealed that the addition of quercetin in the semen extender increased (p < 0.05) frozen-thawed sperm total motility (TM), progressive motility (PM), rapid velocity (RV), average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), and amplitude of lateral head (ALH) displacement in contrast to the control group. Quercetin supplementation had no effect on beat cross frequency (BCF), straightness (STR), and linearity (LIN) (p > 0.05). Quercetin showed significantly higher (p < 0.05) plasma membrane and acrosome integrity and viability (p < 0.05) of spermatozoa in contrast to the control group. Quercetin in the semen extender significantly increased (p < 0.05) superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and total antioxidant capacity (TAC) levels while reduced (p < 0.05) the contents of total oxidant status (TOS) and malondialdehyde (MDA), which were in contrast to the control group. Ultrasound results revealed that 24 out of 30 (80%) goats were found pregnant when semen was treated with 5 µM quercetin while the control group showed 18 out of 30 (60%) animals were pregnant. Thus, the study concluded that 5 µM quercetin-treated semen was found to be efficient, showed increased antioxidant status, and reduced oxidant production, leading to improved spermatozoa quality and in-vivo fertility in goats.

Physicochemical properties and performance of non-woody derived biochars for the sustainable removal of aquatic pollutants: A systematic review.

Biochar is a carbon-rich material produced from the partial combustion of different biomass residues. It can be used as a promising material for adsorbing pollutants from soil and water and promoting environmental sustainability. Extensive research has been conducted on biochars prepared from different feedstocks used for pollutant removal. However, a comprehensive review of biochar derived from non-woody feedstocks (NWF) and its physiochemical attributes, adsorption capacities, and performance in removing heavy metals, antibiotics, and organic pollutants from water systems needs to be included. This review revealed that the biochars derived from NWF and their adsorption efficiency varied greatly according to pyrolysis temperatures. However, biochars (NWF) pyrolyzed at higher temperatures (400-800 °C) manifested excellent physiochemical and structural attributes as well as significant removal effectiveness against antibiotics, heavy metals, and organic compounds from contaminated water. This review further highlighted why biochars prepared from NWF are most valuable/beneficial for water treatment. What preparatory conditions (pyrolysis temperature, residence time, heating rate, and gas flow rate) are necessary to design a desirable biochar containing superior physiochemical and structural properties, and adsorption efficiency for aquatic pollutants? The findings of this review will provide new research directions in the field of water decontamination through the application of NWF-derived adsorbents.

Genome-wide identification and analysis of the cotton ALDH gene family.

BACKGROUND: Aldehyde dehydrogenases (ALDHs) are a family of enzymes that catalyze the oxidation of aldehyde molecules into the corresponding carboxylic acid, regulate the balance of aldehydes and protect plants from the poisoning caused by excessive accumulation of aldehydes; however, this gene family has rarely been studied in cotton. RESULTS: In the present study, genome-wide identification was performed, and a total of 114 ALDH family members were found in three cotton species, Gossypium hirsutum, Gossypium arboreum and Gossypium raimondii. The ALDH genes were divided into six subgroups by evolutionary analysis. ALDH genes in the same subgroup showed similar gene structures and conserved motifs, but some genes showed significant differences, which may result in functional differences. Chromosomal location analysis and selective pressure analysis revealed that the ALDH gene family had experienced many fragment duplication events. Cis-acting element analysis revealed that this gene family may be involved in the response to various biotic and abiotic stresses. The RT‒qPCR results showed that the expression levels of some members of this gene family were significantly increased under salt stress conditions. Gohir.A11G040800 and Gohir.D06G046200 were subjected to virus-induced gene silencing (VIGS) experiments, and the sensitivity of the silenced plants to salt stress was significantly greater than that of the negative control plants, suggesting that Gohir.A11G040800 and Gohir.D06G046200 may be involved in the response of cotton to salt stress. CONCLUSIONS: In total, 114 ALDH genes were identified in three Gossypium species by a series of bioinformatics analysis. Gene silencing of the ALDH genes of G. hirsutum revealed that ALDH plays an important role in the response of cotton to salt stress.

Characteristics and optical properties of atmospheric aerosols based on long-term AERONET investigations in an urban environment of Pakistan.

Radiative balance, local climate, and human health are all significantly influenced by aerosol. Recent severe air pollution over Lahore, a city in Pakistan calls for more thorough research to determine the negative impacts brought on by too many aerosols. To study regional aerosol characteristics and their differences from various aspects, in-depth and long-term (2007-2020) investigations of the columnar aerosol properties over the urban environment of Lahore were carried out by using AERONET data. The Aerosol Optical Depth (AOD400) and Angstrom Exponent (AE400-870) vary from low values of 0.10 to a maximum value of 4.51 and from 0.03 to 1.81, respectively. The huge differences in the amount of AOD440 as well as AE440-870 show the large fluctuation of aerosol classes because of various sources of their emission. During the autumn and winter seasons, the decreasing trend of the optical parameters of aerosols like Single Scattering Albedo (SSA) and Asymmetry Parameter (ASY) with increasing wavelength from 675 to 1020 nm indicates the dominance of light-absorbing aerosols (biomass burning (BB) and industrial/urban (UI). Due to the long-distance dust movement during spring, summer, and autumn, coarse mode particles predominated in Lahore during the study period. Dust type (DD) aerosols are found to be the dominant one during spring (46.92%), summer (54.31%), and autumn (57.46%) while urban industry (BB/UI) was dominant during the winter season (53.21%). During each season, the clean continental (CC) aerosols are found to be in negligible amounts, indicating terrible air quality in Lahore City. The present research work fills up the study gap in the optical properties of aerosols in Lahore and will help us understand more fully how local aerosol fluctuation affects regional climate change over the urban environment of Lahore.

Geographical distribution of radon and associated health risks in drinking water samples collected from the Mulazai area of Peshawar, Pakistan.

Geospatial methods, such as GIS and remote sensing, map radon levels, pinpoint high-risk areas and connect geological traits to radon presence. These findings direct health planning, focusing tests, mitigation, and policies where radon levels are high. Overall, geospatial analyses offer vital insights, shaping interventions and policies to reduce health risks from radon exposure. There is a formidable threat to human well-being posed by the naturally occurring carcinogenic radon (222Rn) gas due to high solubility in water. Under the current scenario, it is crucial to assess the extent of 222Rn pollution in our drinking water sources across various regions and thoroughly investigate the potential health hazards it poses. In this regard, the present study was conducted to investigate the concentration of 222Rn in groundwater samples collected from handpumps and wells and to estimate health risks associated with the consumption of 222Rn-contaminated water. For this purpose, groundwater samples (n = 30) were collected from handpumps, and wells located in the Mulazai area, District Peshawar. The RAD7 radon detector was used as per international standards to assess the concentration of 222Rn in the collected water samples. The results unveiled that the levels of 222Rn in the collected samples exceeded the acceptable thresholds set by the US Environmental Protection Agency (US-EPA) of 11.1 Bq L-1. Nevertheless, it was determined that the average annual dose was below the recommended limit of 0.1 mSv per year, as advised by both the European Union Council and the World Health Organization. In order to avoid the harmful effects of such excessive 222Rn concentrations on human health, proper ventilation and storage of water in storage reservoirs for a long time before use is recommended to lower the 222Rn concentration.

Metal Oxide-Impregnated Biochar for Azo Dye Remediation as Revealed through Kinetics, Thermodynamics, and Response Surface Methodology.

This study reports for the first time the adsorption capacity of a novel adsorbent Croton bonplandianus Baill. biochar. Its adsorption capacity was further enhanced by loading magnetic composites on it, which makes it an efficient medium for the adsorption of dyes. Two azo dyes, Basic Brown 1 (BB1) and Basic Orange 2 (BO2), were studied for their effective adsorption from aqueous media. A comprehensive characterization was performed by using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) to study the properties of Fe2O3-loaded C. bonplandianus Baill. biochar (FO-CBPBB). A series of batch experiments were conducted to optimize various parameters (pH, contact time, adsorbent amount, initial BB1 and BO2 concentrations, and temperature) for the maximum adsorption of BB1 and BO2 on the FO-CBPBB adsorbent. The percentage of BB1 and BO2 dyes that adsorb to FO-CBPBB under the best experimental circumstances (pH of solution 7, contact time 80 min, temperature of solution 40 °C, initial BB1 and BO2 dye concentrations 80 mg L-1, and adsorbent dose 1 g L-1) was 93 and 95%, respectively. The best adsorption of BB1 and BO2 was accomplished by optimizing the effects of several factors, including the starting dye concentration, contact time, and temperature, based on the central composite design. The Freundlich and Langmuir isotherm models were used to examine the equilibrium data. The Langmuir isotherm with the greatest adsorption capacity and R2 value effectively captured the experimental results. When kinetic parameters were investigated, it was found that pseudo-second-order was appropriate, reflecting the fact that the dye-adsorbent interaction was the rate-controlling factor in this study. The sorption process was endothermic and spontaneous, as shown by the thermodynamic variables. Based on the interaction between the adsorbent and azo dyes, it was concluded that the adsorption process was electrostatic in nature. Adsorbents that have been synthesized can effectively remove azo dyes from wastewater. Excellent regeneration efficiency was exhibited by FO-CBPBB, which makes it an eco-friendly and cost-effective alternative to other costly techniques applied for water purification.

Wintertime investigation of PM10 concentrations, sources, and relationship with different meteorological parameters.

Meteorological factors play a crucial role in affecting air quality in the urban environment. Peshawar is the capital city of the Khyber Pakhtunkhwa province in Pakistan and is a pollution hotspot. Sources of PM10 and the influence of meteorological factors on PM10 in this megacity have yet to be studied. The current study aims to investigate PM10 mass concentration levels and composition, identify PM10 sources, and quantify links between PM10 and various meteorological parameters like temperature, relative humidity (RH), wind speed (WS), and rainfall (RF) during the winter months from December 2017 to February 2018. PM10 mass concentrations vary from 180 - 1071 µg m-3, with a mean value of 586 ± 217 µg m-3. The highest concentration is observed in December, followed by January and February. The average values of the mass concentration of carbonaceous species (i.e., total carbon, organic carbon, and elemental carbon) are 102.41, 91.56, and 6.72 µgm-3, respectively. Water-soluble ions adhere to the following concentration order: Ca2+ > Na+ > K+ > NH4+ > Mg2+. Twenty-four elements (Al, Si, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Co, Zn, Ga, Ge, As, Se, Kr, Ag, Pb, Cu, and Cd) are detected in the current study by PIXE analysis. Five sources based on Positive Matrix Factorization (PMF) modeling include industrial emissions, soil and re-suspended dust, household combustion, metallurgic industries, and vehicular emission. A positive relationship of PM10 with temperature and relative humidity is observed (r = 0.46 and r = 0.56, respectively). A negative correlation of PM10 is recorded with WS (r = - 0.27) and RF (r = - 0.46). This study's results motivate routine air quality monitoring owing to the high levels of pollution in this region. For this purpose, the establishment of air monitoring stations is highly suggested for both PM and meteorology. Air quality standards and legislation need to be revised and implemented. Moreover, the development of effective control strategies for air pollution is highly suggested.

Identification and function analysis of GABA branch three gene families in the cotton related to abiotic stresses.

γ -aminobutyric acid (GABA) is closely related to the growth, development and stress resistance of plants. Combined with the previous study of GABA to promote the cotton against abiotic stresses, the characteristics and expression patterns of GABA branch gene family laid the foundation for further explaining its role in cotton stress mechanism. Members of GAD, GAB-T and SSADH (three gene families of GABA branch) were identified from the Gossypium hirsutum, Gossypium barbadense, Gossypium arboreum and Gossypium raimondii genome. The GABA branch genes were 10 GAD genes, 4 GABA-T genes and 2 SSADH genes. The promoter sequences of genes mainly contains response-related elements such as light, hormone and environment.Phylogenetic analysis shows that GAD indicating that even in the same species, the homologous sequences in the family. The GABA-T gene of each cotton genus was in sum the family had gene loss in the process of dicotyledon evolution. SSADH families Gossypium hirsutum, Gossypium barbadense, Gossypium arboreum and Gossypium raimondii were closely related to the dicot plants.GABA gene is involved in the regulation of salt stress and high temperature in Gossypium hirsutum.GABA attenuated part of the abiotic stress damage by increasing leaf protective enzyme activity and reducing reactive oxygen species production.This lays the foundation for a thorough analysis of the mechanism of GABA in cotton stress resistance.

Nanoparticles in plant resistance against bacterial pathogens: current status and future prospects.

Nanoparticles (NPs) serve immense roles in various fields of science. They have vastly upgraded conventional methods in the fields of agriculture and food sciences to eliminate growing threats of crop damage and disease, caused by various phytopathogens including bacteria, fungi, viruses, and some insects. Bacterial diseases resulted in mass damage of crops by adopting antibacterial resistance, which has proved to be a major threat leading to food scarcity. Therefore, numerous NPs with antibacterial potentials have been formulated to overcome the problem of antibiotic resistance alongside an increase in crop yield and boosting plant immunity. NPs synthesized through green synthesis techniques have proved to be more effective and environment-friendly than those synthesized via chemical methods. NPs exhibit great roles in plants ranging from enhanced crop yield to disease suppression, to targeted drug and pesticide deliveries inside the plants and acting as biosensors for pathogen detection. NPs serves major roles in disruption of cellular membranes, ROS production, altering of DNA and protein entities and changing energy transductions. This review focuses on the antibacterial effect of NPs on several plant bacterial pathogens, mostly, against Pseudomonas syringe, Ralstonia solanacearum, Xanthomonas axonopodis, Clavibacter michiganensisand Pantoea ananatis both in vivo and ex vivo, thereby minimizing their antibacterial resistance and enhancing the plants acquired immunity. Therefore, NPs present a safer and more reliable bactericidal activity against various disease-causing bacteria in plants.

Recent trends and economic significance of modified/functionalized biochars for remediation of environmental pollutants.

The pollution of soil and aquatic systems by inorganic and organic chemicals has become a global concern. Economical, eco-friendly, and sustainable solutions are direly required to alleviate the deleterious effects of these chemicals to ensure human well-being and environmental sustainability. In recent decades, biochar has emerged as an efficient material encompassing huge potential to decontaminate a wide range of pollutants from soil and aquatic systems. However, the application of raw biochars for pollutant remediation is confronting a major challenge of not getting the desired decontamination results due to its specific properties. Thus, multiple functionalizing/modification techniques have been introduced to alter the physicochemical and molecular attributes of biochars to increase their efficacy in environmental remediation. This review provides a comprehensive overview of the latest advancements in developing multiple functionalized/modified biochars via biological and other physiochemical techniques. Related mechanisms and further applications of multiple modified biochar in soil and water systems remediation have been discussed and summarized. Furthermore, existing research gaps and challenges are discussed, as well as further study needs are suggested. This work epitomizes the scientific prospects for a complete understanding of employing modified biochar as an efficient candidate for the decontamination of polluted soil and water systems for regenerative development.

An analysis of lncRNAs related to fiber quality and the discovery of their target genes in a Gossypium hirsutum line with Gossypium mustelinum introgression.

KEY MESSAGE: Analysis of fiber quality lncRNAs and their target genes from a pair of Gossypium mustelinum near-isogenic lines provide new prospects for improving the fiber quality of Upland cotton. Long noncoding RNAs (lncRNAs) are an important part of genome transcription and play roles in a wide range of biological processes in plants. In this research, a pair of near-isogenic cotton lines, namely, a Gossypium mustelinum introgression line (IL9) with outstanding fiber quality and its recurrent Upland cotton parent (PD94042), were used as the experimental materials. Cotton fibers were selected for lncRNA sequencing at 17 and 21 days post-anthesis. A total of 2693 differentially expressed genes were identified. In total, 5841 lncRNAs were ultimately screened, from which 163 differentially expressed lncRNAs were identified. Target genes of the lncRNAs were predicted by two different methods: cis and trans. Some of the target genes were related to cell components, membrane components, plant hormone signal transduction and catalytic metabolism, and the results indicated that there might also be important effects on the development of fiber. Four differentially expressed target genes related to fiber quality (Gomus.D05G015100, Gomus.A05G281300, Gomus.A12G023400 and Gomus.A10G226800) were screened through gene function annotation, and the functions of these four genes were verified through virus-induced gene silencing (VIGS). Compared to the negative controls, plants in which any of these four genes were silenced showed significant reductions in fiber strength. In addition, the plants in which the Gomus.A12G023400 gene was silenced showed a significant reduction in fiber uniformity, whereas the plants in which Gomus.A05G281300 was silenced showed a significant increase in fiber fineness as measured via micronaire. Our results showed that these genes play different roles during fiber development, impacting fiber quality.

Decoding the guardians of cotton resilience: A comprehensive exploration of the ßCA genes and its role in Verticillium dahliae resistance.

Plant Carbonic anhydrases (Cas) have been shown to be stress-responsive enzymes that may play a role in adapting to adverse conditions. Cotton is a significant economic crop in China, with upland cotton (Gossypium hirsutum) being the most widely cultivated species. We conducted genome-wide identification of the ßCA gene in six cotton species and preliminary analysis of the ßCA gene in upland cotton. In total, 73 ßCA genes from six cotton species were identified, with phylogenetic analysis dividing them into five subgroups. GHßCA proteins were predominantly localized in the chloroplast and cytoplasm. The genes exhibited conserved motifs, with motifs 1, 2, and 3 being prominent. GHßCA genes were unevenly distributed across chromosomes and were associated with stress-responsive cis-regulatory elements, including those responding to light, MeJA, salicylic acid, abscisic acid, cell cycle regulation, and defence/stress. Expression analysis indicated that GHßCA6, GHßCA7, GHßCA10, GHßCA15, and GHßCA16 were highly expressed under various abiotic stress conditions, whereas GHßCA3, GHßCA9, GHßCA10, and GHßCA18 had higher expression patterns under Verticillium dahliae infection at different time intervals. In Gossypium thurberi, GthßCA1, GthßCA2, and GthßCA4 showed elevated expression across stress conditions and tissues. Silencing GHßCA10 through VIGS increased Verticillium wilt severity and reduced lignin deposition compared to non-silenced plants. GHßCA10 is crucial for cotton's defense against Verticillium dahliae. Further research is needed to understand the underlying mechanisms and develop strategies to enhance resistance against Verticillium wilt.

Biosorption Potential of Arachis hypogaea-Derived Biochar for Cd and Ni, as Evidenced through Kinetic, Isothermal, and Thermodynamics Modeling.

Biochar derived from plant biomass has great potential for the decontamination of aqueous media. It is the need of the hour to test biochar derived from economical, easily available, and novel materials. In this regard, the present study provides insight into the sorption of two heavy metals, i.e., cadmium (Cd) and nickel (Ni), using native Arachis hypogaea and its biochar prepared through pyrolysis. The effect of different factors, including interaction time, initial concentration of adsorbate, and temperature, as well as sorbent dosage, was studied on the sorption of Cd and Ni through a batch experiment. Characterization of the native biowaste and prepared biochar for its surface morphology and functional group identification was executed using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). Results revealed the presence of different functional groups such as -OH on the surface of the adsorbent, which plays an important role in metal attachment. SEM reveals the irregular surface morphology of the adsorbent, which makes it easy for metal attachment. Thermogravimetric analysis shows the stability of A. hypogaea biochar up to 380 °C as compared with native adsorbent. The adsorption efficacy of A. hypogaea was found to be higher than that of native A. hypogaea for both metals. The best adsorption of Cd (94.5%) on biochar was observed at a concentration of 40 ppm, an adsorbent dosage of 2 g, a contact time of 100 min, and a temperature of 50 °C. While the optimum conditions for adsorption of Ni on biochar (97.2% adsorption) were reported at a contact time of 100 min, adsorbent dosage of 2.5 g, initial concentration of 60 ppm, and temperature of 50 °C. Results revealed that biochar offers better adsorption of metal ions as compared with raw samples at low concentrations. Isothermal studies show the adsorption mechanism as physical adsorption, and the negative value of Gibb's free energy confirms the spontaneous nature of the adsorption reaction. An increase in entropy value favors the adsorption process. Results revealed that the sorbent was a decent alternative to eliminate metal ions from the solution instead of costly adsorbents.

Adsorption potential of orange rind-based nanosorbents for the removal of cadmium(II) and chromium(VI) from contaminated water.

Heavy metals (HMs) in water are highly poisonous and carcinogenic agents for human health. To alleviate the toxic impacts of HMs, green remediation technologies are the need of the hour. In this regard, different nanosorbents (CMCG@ORP, ORAC, NiO/NPs, and NiO@ORAC/NCs) were synthesized in the present study, and the percentage removal of heavy metals [chromium(VI) and cadmium(II) ions] was evaluated. The nanosorbents were characterized by using FTIR, SEM, UV-Vis spectroscopy, and XRD. UV-Vis spectroscopy confirmed the synthesis of nanosorbents such as NiO/NPs and NiO@ORAC/NCs at 330.5 nm and 352.55 nm, respectively. The characterization studies show that the surface of synthesized nano-sorbents was highly coarse, uneven, and abrasive. XRD pattern deduced that the sample was of single phase, and no other impurity was detected except the face-centered cubic-phase peak of NiO. The maximum adsorption of Cd (91%) and Cr (92%) was found at initial concentrations of 100 and 60 ppm respectively at contact time = 180 min, temperature 25 °C, and with an adsorbent dose of 0.5 g. Isothermal, kinetic, and thermodynamic studies were also performed to evaluate the adsorption mechanisms and feasibility of the process. Adsorption mostly followed Freundlich isotherm which indicates the multilayer adsorption phenomenon and the negative value of Gibb's free energy showed the spontaneous nature and feasibility of the adsorption reaction. Surface complexation, ion exchange, surface precipitation, and the phenomenon of physical adsorption occurred on the sorbent surface which led to the attachment of Cd and Cr to the tested nanosorbents. In conclusion, NiO@ORAC/NCs were the most effective in the alleviation of Cd(II) and Cr(VI) ions in contaminated water.

Drought-Induced miRNA Expression Correlated with Heavy Metal, Phenolic Acid, and Protein and Nitrogen Levels in Five Chickpea Genotypes.

Drought is a prime stress, drastically affecting plant growth, development, and yield. Plants have evolved various physiological, molecular, and biochemical mechanisms to cope with drought. Investigating specific biochemical pathways related to drought tolerance mechanisms of plants through biotechnology approaches is one of the quickest and most effective strategies for enhancing crop production. Among them, microRNAs (miRNAs) are the principal post-transcriptional regulators of gene expression in plants during plant growth under biotic and abiotic stresses. In this study, five different chickpea genotypes (Inci, Hasan bey, Arda, Seçkin, and Diyar 95) were grown under normal and drought stress. We recorded the expression levels of microRNAs in these genotypes and found differential expression (miRNA396, miR408, miRNA414, miRNA528, and miRNA1533) under contrasting conditions. Results revealed that miRNA414 and miRNA528 considerably increased in all genotypes under drought stress, and expression levels of miRNA418, miRNA1533, and miRNA396 (except for the Seçkin genotype) were found to be higher under the watered conditions. These genotypes were also investigated for heavy metal, phenolic acid, protein, and nitrogen concentrations under normal and drought stress conditions. The Arda genotype showed a significant increase in nitrogen (5.46%) and protein contents (28.3%), while protein contents were decreased in the Hasan bey and Seçkin genotypes subjected to drought stress. In the case of metals, iron was the most abundant element in all genotypes (Inci = 15.4 ppm, Hasan bey = 29.6 ppm, Seçkin = 37.8 ppm, Arda = 26.3 ppm, and Diyar 95 = 40.8 ppm) under normal conditions. Interestingly, these results were related to miRNA expression in the chickpea genotypes and hint at the regulation of multiple pathways under drought conditions. Overall, the present study will help us to understand the miRNA-mediated regulation of various pathways in chickpea genotypes.

Comparative Efficacy of Foliar Plus Soil Application of Urea versus Conventional Application Methods for Enhanced Growth, Yield, Agronomic Efficiency, and Economic Benefits in Rice.

The experiment was conducted at the research field, Department of Agronomy, Hajee Mohammad Danesh Science and Technology University, Dinajpur from December 2017 to May 2018 to find out the best treatment of foliar application of urea on the growth and yield of boro rice cv. BRRI dhan28. The experiment consisted of 10 treatments, laid out in a randomized complete block design in triplicate. The recommended doses (RD) of urea, TSP, MOP, gypsum, ZnSO4, and borax were applied during land preparation except for urea at 250, 75, 100, 75, 7, and 5 kg ha-1, respectively, where urea was applied as per treatment specification. The results revealed that the application of N fertilizer as foliage along with soil significantly influenced the growth, plant characteristics, and yield of BRRI dhan28. There was no significant difference between T8 (70% in soil and 10% as foliage) and T9 (100% in soil) treatment regarding the maximum panicle length (21.43 and 20.71 cm), fertile grains (117.40 and 113.30), total grains (134.40 and 130.97), 1000-grain weight (24.56 and 23.56 g), grain yield (5.91 and 5.74 t ha-1), straw yield (7.83 and 7.92 t ha-1), biological yield (13.74 and 13.66 t ha-1), and harvest index (43.01 and 42.02%), respectively, in this study. These results indicated that N fertilization as direct soil application (70%) and as foliage application (10%), i.e., 80% N fertilization, produced the highest grain yield and major yield traits which we received by 100% N fertilization as soil that was practiced traditionally by the farmers. The effect of overfertilization (T10) was not positive, producing the highest number of noneffective tillers and sterile grains (nonfilled grains). Therefore, it is possible to achieve an equivalent or more yield by saving 20% urea by the combination of soil (70%) and foliage (10%) application as compared to the traditional method of fertilizer application (100% in soil).

Genome-wide analysis of the LAZ1 gene family in Gossypium hirsutum.

BACKGROUND: As the world's leading fiber crop and a major oil-producing crop, cotton fiber yield and fiber quality are affected by environmental stresses, especially heat, drought and salinity. The LAZ1 (Lazarus 1) family genes are responsive to abscisic acid, drought, and salt treatments. Currently, mining and functional analyses of LAZ1 family genes in cotton have not been reported. METHODS AND RESULTS: In this study, 20 GhLAZ1 genes, designated GhLAZ1-1 - GhLAZ1-20, were identified in the genome of Gossypium hirsutum through the construction of an HMM model, and their molecular properties, chromosomal localization, phylogeny, gene structure, evolutionary selection pressure, promoter cis elements and gene expression under salt stress were analyzed. With the exception of GhLAZ1-17 and GhLAZ1-20, the remaining 18 GhLAZ1 genes were unevenly localized on 13 chromosomes in G. hirsutum; evolutionary analysis showed that these genes could be divided into three subfamilies; and evolutionary selection pressure analysis demonstrated that the GhLAZ1 genes were all under purifying selection. Many elements related to light responses, hormone responses, and abiotic stresses were predicted on the GhLAZ1 family gene promoters, and real-time quantitative PCR results showed that GhLAZ1-2, GhLAZ1-8, and GhLAZ1-18 were upregulated significantly in salt-treated cotton leaves. CONCLUSIONS: Our results suggested that GhLAZ1 genes were involved in the salt tolerance mechanism in G. hirsutum and provided a reference for further exploring the function and molecular mechanism of LAZ1 genes.

Morphological, Mineralogical, and Biochemical Characteristics of Particulate Matter in Three Size Fractions (PM10, PM2.5, and PM1) in the Urban Environment.

Air pollution in megacities is increasing due to the dense population index, increasing vehicles, industries, and burning activities that negatively impact human health and climate. There is limited study of air pollution in many megacities of the world including Pakistan. Lahore is a megacity in Pakistan in which the continuous investigation of particulate matter is very important. Therefore, this study investigates particulate matter in three size fractions (PM1, PM2.5, and PM10) in Lahore, a polluted city in south Asia. The particulate matter was collected daily during the winter season of 2019. The average values of PM1, PM2.5, and PM10 were found to be 102.00 ± 64.03, 188.31 ± 49.21, and 279.73 ± 75.04 µg m-3, respectively. Various characterization techniques including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM) combined with energy-dispersive X-ray spectroscopy (EDX) were used. FT-IR and XRD techniques identified the minerals and compounds like quartz, peroxides, calcites and vaterite, feldspar group, kaolinite clay minerals, chrysotile, vaterite, illite, hematite, dolomite, calcite, magnesium phosphate, ammonium sulfate, calcium iron oxide, gypsum, vermiculite, CuSO4, and FeSO4. Morphology and elemental composition indicated quartz, iron, biological particles, carbonate, and carbonaceous particles. In addition, various elements like C, O, B, Mg, Si, Ca, Cl, Al, Na, K, Zn, and S were identified. Based on the elemental composition and morphology, different particles along with their percentage were found like carbonaceous- (38%), biogenic- (14%), boron-rich particle- (14%), feldspar- (10%), quartz- (9%), calcium-rich particle- (5%), chlorine-rich particle- (5%), and iron-rich particle (5%)-based. The main sources of the particulate matter included vehicular exertion, biomass consumption, resuspended dust, biological emissions, activities from construction sites, and industrial emissions near the sampling area.

Exploring the recuperative potential of brassinosteroids and nano-biochar on growth, physiology, and yield of wheat under drought stress.

Drought stress as a result of rapidly changing climatic conditions has a direct negative impact on crop production especially wheat which is the 2nd staple food crop. To fulfill the nutritional demand under rapidly declining water resources, there is a dire need to adopt a precise, and efficient approach in the form of different amendments. In this regard, the present study investigated the impact of nano-biochar (NBC) and brassinosteroids (BR) in enhancing the growth and productivity of wheat under different drought stress conditions. The field study comprised different combinations of amendments (control, NBC, BR, and NBC + BR) under three irrigation levels (D0, D1 and D2). Among different treatments, the synergistic approach (NBC + BR) resulted in the maximum increase in different growth and yield parameters under normal as well as drought stress conditions. With synergistic approach (NBC + BR), the maximum plant height (71.7 cm), spike length (17.1), number of fertile tillers m-2 (410), no. of spikelets spike-1 (19.1), no. of grains spike-1 (37.9), 1000 grain weight (37 g), grain yield (4079 kg ha-1), biological yield (10,502 kg ha-1), harvest index (43.5). In the case of physiological parameters such as leaf area index, relative water contents, chlorophyll contents, and stomatal conductance were maximally improved with the combined application of NBC and BR. The same treatment caused an increase of 54, 10, and 7% in N, P, and K contents in grains, respectively compared to the control treatment. Similarly, the antioxidant response was enhanced in wheat plants under drought stress with the combined application of NBC and BR. In conclusion, the combined application of NBC and BR caused a significant increase in the growth, physiological and yield attributes of wheat under drought stress.