Irradiation with 365 nm and 405 nm wavelength shows differences in DNA damage of swine pancreatic islets.

INTRODUCTION: 3D printing is being used more extensively in modern biomedicine. One of the problems is selecting a proper crosslinking method of bioprinted material. Amongst currently used techniques we can distinguish: physical crosslinking (e.g. Ca2+ and Sr2+) and chemical crosslinking-the UV light crosslinking causing the biggest discussion. UV radiation is selectively absorbed by DNA, mainly in the UV-B region but also (to some extent) in UV-A and UV-C regions. DNA excitement results in typical photoproducts. The amount of strand breaks may vary depending on the period of exposition, it can also differ when cells undergo incubation after radiation. AIM: The aim of this study was to show whether and how the time of irradiation with 405 nm and 365 nm wavelengths affect DNA damage in cell lines and micro-organs (pancreatic islets). MATERIALS AND METHODS: The degree of DNA damage caused by different wavelengths of radiation (405 nm and 365 nm) was evaluated by a comet assay. The test was performed on fibroblasts, alpha cells, beta cells and porcine pancreatic islets after 24 hours incubation period. Samples without radiation treatment were selected as a control group. Results analysis consisted of determining the percent of cells with damaged DNA and the tail intensity evaluation. RESULTS: The degree of DNA damage in pancreatic islets after exposure to 405 nm wavelength oscillated between 2% and 6% depending on the tested time period (10 - 300 seconds). However, treating islets using 365 nm wavelength resulted in damage up to 50%. This clearly shows significantly less damage when using 405 nm wavelength. Similar results were obtained for the tested cell lines. CONCLUSIONS: Crosslinking with 405 nm is better for pancreatic islets than crosslinking with 365 nm UV light.

Chronoendocrinological studies in athletes. III. Certain psychoendocrinological correlations.

In 83 healthy athletes, statistically highly significant circadian rhythms of psychological parameters (as measured with the SAN-questionnaire [8]: feelings of well-being, activation [1], mood) have been validated and quantified by cosinor analysis. There were significant correlations between some personality factors, as measured with Cattell 16PF questionnaire, in 32 fencers, and some mean circadian hormone blood concentrations. No significant correlations have been found between parameters measured with Eysenck test or with the D48 test and the mean circadian blood levels of T3, TSH, CSL, T, HGH, IRI.

Chromoendocrinological studies in athletes. IV. Multiple hormonal responses to submaximal muscular exercise.

Responses of serum hormones and factors measured with the SAN-test (feelings of well-being, activation, mood) to a submaximal work load in 83 health athletes were expressed in per cent of the values expected individually according to the respective circadian rhythm [5,6,7]. In the most subjects a significant increase of serum HGH and a significant decrease of serum T (= testosterone) was found under the work load. Within 45 min after the completion of the work the level of HGH returned to the expected one, according to the circadian rhythm, while the level of T did not. Some other minor hormonal responses were observed. No significant differences were shown between the actual values of factors measured with the SAN-test and the respective values expected according to the circadian rhythm.

Chronoendocrinological studies in athletes. Circadian rhythms of HGH and IRI.

The serum concentrations of HGH and IRI studied over a 24-hour span in 83 healthy athletes under normal rest conditions in a normal living environment show statistically significant rhythms, validated and quantified by cosinor analysis; for each hormone three different types of the circadian rhythm (with a peak of acrophase between: 6.30 a.m. and 1.30 p.m., 1.30 p.m. and 10.30 p.m., 10.30 p.m. and 6.30 a.m.) were observed. The IRI response to a meal load underwent cyclic variations during the day.

Chronoendocrinological studies in athletes. Circadian rhythms of T3, TSH, cortisol and testosterone.

Endocrine functions were studied in 83 healthy athletes, maintained under normal light-dark conditions, with normal activity-sleep patterns. In these subjects statistically significant circadian rhythms of T3, TSH, cortisol (CSL) and testosterone (T) were found; for each hormone two or three different types of circadian rhythm (with peaks of acrophase between: 6.30 a.m. and 1.30 a.m., 1.30 p.m. and 10.30 p.m., 10.30 p.m. and 6.30 p.m.) were observed. The following correlations have been demonstrated between the length of participation (in years) in performance sports and the mean circadian blood hormone concentration: a negative one for CSL (p less than 0.01) and a positive one for TSH (p less than 0.05). An increase of the mean circadian values of T concentration in venous blood was found in females fencers (n = 9).

Plasma immunoreactive insulin and somatotropin in delirium tremens and alcoholic hallucinosis.

The glucose tolerance curve in alcoholics in delirium tremens was similar to that seen in hepatogenic diabetes. The secretion of immunoreactive insulin and somatotropin after glucose was similar in patients with delirium tremens and alcoholic hallucinosis.

[Plasma ACTH, STH and other hormone levels in various groups under chlormethiazole, haloperidol or reserpine load in alchohol delirium, alcoholic hallucinations, and chronic alcoholism]. / Untersuchungen über den Gehalt des Plasmas an ACTH, an STH und an anderen Hormonen unter Belastung in verschiedenen Gruppen mit Chlormethiazol, Haloperidol oder Reserpin bei Alkoholdelir, alkoholischen Halluzinosen und chronischen Alkoholikern

Studies of 135 men with safely diagnosed alcohol delirium mostly revealed increased ACTH blood values when sober and increased T4 values in about 1/3 of these patients. There is a correlation between the psychiatric clinical picture of the alcohol delirium and the ACTH content of the plasma. Under load with chloromethiazole, halperidole or with reserpine, there is a significant drop in the increased ACTH and T4 values. In an acute alcoholic hallucinosis (n=16) similar endocrinological changes as in most cases of safely diagnosed alcohol delirium were observed. In a chronic alcoholic hallucinosis (n=11) and in chronic alcoholics (n=31) the endocrinological values were similar to those of patients after alcohol delirium.