ABSTRACT
In Toponomics, the function protein pattern in cells or tissue (the toponome) is imaged and analyzed for applications in toxicology, new drug development and patient-drug-interaction. The most advanced imaging technique is robot-driven multi-parameter fluorescence microscopy. This technique is capable of co-mapping hundreds of proteins and their distribution and assembly in protein clusters across a cell or tissue sample by running cycles of fluorescence tagging with monoclonal antibodies or other affinity reagents, imaging, and bleaching in situ. The imaging results in complex multi-parameter data composed of one slice or a 3D volume per affinity reagent. Biologists are particularly interested in the localization of co-occurring proteins, the frequency of co-occurrence and the distribution of co-occurring proteins across the cell. We present an interactive visual analysis approach for the evaluation of multi-parameter fluorescence microscopy data in toponomics. Multiple, linked views facilitate the definition of features by brushing multiple dimensions. The feature specification result is linked to all views establishing a focus+context visualization in 3D. In a new attribute view, we integrate techniques from graph visualization. Each node in the graph represents an affinity reagent while each edge represents two co-occurring affinity reagent bindings. The graph visualization is enhanced by glyphs which encode specific properties of the binding. The graph view is equipped with brushing facilities. By brushing in the spatial and attribute domain, the biologist achieves a better understanding of the function protein patterns of a cell. Furthermore, an interactive table view is integrated which summarizes unique fluorescence patterns. We discuss our approach with respect to a cell probe containing lymphocytes and a prostate tissue section.
Subject(s)
Computer Graphics , Microscopy, Fluorescence/statistics & numerical data , Proteomics/statistics & numerical data , Data Interpretation, Statistical , Humans , Imaging, Three-Dimensional/statistics & numerical data , Lymphocytes/metabolism , Male , Neoplasm Proteins/metabolism , Prostatic Neoplasms/metabolismABSTRACT
This paper presents a novel framework for visualizing volumetric data specified on complex polyhedral grids, without the need to perform any kind of a priori tetrahedralization. These grids are composed of polyhedra that often are non-convex and have an arbitrary number of faces, where the faces can be non-planar with an arbitrary number of vertices. The importance of such grids in state-of-the-art simulation packages is increasing rapidly. We propose a very compact, face-based data structure for representing such meshes for visualization, called two-sided face sequence lists (TSFSL), as well as an algorithm for direct GPU-based ray-casting using this representation. The TSFSL data structure is able to represent the entire mesh topology in a 1D TSFSL data array of face records, which facilitates the use of efficient 1D texture accesses for visualization. In order to scale to large data sizes, we employ a mesh decomposition into bricks that can be handled independently, where each brick is then composed of its own TSFSL array. This bricking enables memory savings and performance improvements for large meshes. We illustrate the feasibility of our approach with real-world application results, by visualizing highly complex polyhedral data from commercial state-of-the-art simulation packages.
ABSTRACT
We present a systematic approach to the interactive visual analysis of heterogeneous scientific data. The data consist of two interrelated parts given on spatial grids over time (e.g., atmosphere and ocean part from a coupled climate model). By integrating both data parts in a framework of coordinated multiple views (with linking and brushing), the joint investigation of features across the data parts is enabled. An interface is constructed between the data parts that specifies 1) which grid cells in one part are related to grid cells in the other part, and vice versa, 2) how selections (in terms of feature extraction via brushing) are transferred between the two parts, and 3) how an update mechanism keeps the feature specification in both data parts consistent during the analysis. We also propose strategies for visual analysis that result in an iterative refinement of features specified across both data parts. Our approach is demonstrated in the context of a complex simulation of fluid-structure interaction and a multirun climate simulation.
ABSTRACT
Rhinologists are often faced with the challenge of assessing nasal breathing from a functional point of view to derive effective therapeutic interventions. While the complex nasal anatomy can be revealed by visual inspection and medical imaging, only vague information is available regarding the nasal airflow itself: Rhinomanometry delivers rather unspecific integral information on the pressure gradient as well as on total flow and nasal flow resistance. In this article we demonstrate how the understanding of physiological nasal breathing can be improved by simulating and visually analyzing nasal airflow, based on an anatomically correct model of the upper human respiratory tract. In particular we demonstrate how various Information Visualization (InfoVis) techniques, such as a highly scalable implementation of parallel coordinates, time series visualizations, as well as unstructured grid multi-volume rendering, all integrated within a multiple linked views framework, can be utilized to gain a deeper understanding of nasal breathing. Evaluation is accomplished by visual exploration of spatio-temporal airflow characteristics that include not only information on flow features but also on accompanying quantities such as temperature and humidity. To our knowledge, this is the first in-depth visual exploration of the physiological function of the nose over several simulated breathing cycles under consideration of a complete model of the nasal airways, realistic boundary conditions, and all physically relevant time-varying quantities.
Subject(s)
Computer Graphics , Computer Simulation , Image Processing, Computer-Assisted/methods , Models, Anatomic , Nose/anatomy & histology , Pulmonary Ventilation/physiology , Humans , Humidity , Nose/diagnostic imaging , Nose/physiology , Respiration , Temperature , Tomography, X-Ray ComputedABSTRACT
One of the most prominent topics in climate research is the investigation, detection, and allocation of climate change. In this paper, we aim at identifying regions in the atmosphere (e.g., certain height layers) which can act as sensitive and robust indicators for climate change. We demonstrate how interactive visual data exploration of large amounts of multi-variate and time-dependent climate data enables the steered generation of promising hypotheses for subsequent statistical evaluation. The use of new visualization and interaction technology--in the context of a coordinated multiple views framework--allows not only to identify these promising hypotheses, but also to efficiently narrow down parameters that are required in the process of computational data analysis. Two datasets, namely an ECHAM5 climate model run and the ERA-40 reanalysis incorporating observational data, are investigated. Higher-order information such as linear trends or signal-to-noise ratio is derived and interactively explored in order to detect and explore those regions which react most sensitively to climate change. As one conclusion from this study, we identify an excellent potential for usefully generalizing our approach to other, similar application cases, as well.
ABSTRACT
Perfusion data are dynamic medical image data which characterize the regional blood flow in human tissue. These data bear a great potential in medical diagnosis, since diseases can be better distinguished and detected at an earlier stage compared to static image data. The wide-spread use of perfusion data is hampered by the lack of efficient evaluation methods. For each voxel, a time-intensity curve characterizes the enhancement of a contrast agent. Parameters derived from these curves characterize the perfusion and have to be integrated for diagnosis. The diagnostic evaluation of this multi-field data is challenging and time-consuming due to its complexity. For the visual analysis of such datasets, feature-based approaches allow to reduce the amount of data and direct the user to suspicious areas. We present an interactive visual analysis approach for the evaluation of perfusion data. For this purpose, we integrate statistical methods and interactive feature specification. Correlation analysis and Principal Component Analysis (PCA) are applied for dimensionreduction and to achieve a better understanding of the inter-parameter relations. Multiple, linked views facilitate the definition of features by brushing multiple dimensions. The specification result is linked to all views establishing a focus+context style of visualization in 3D. We discuss our approach with respect to clinical datasets from the three major application areas: ischemic stroke diagnosis, breast tumor diagnosis, as well as the diagnosis of the coronary heart disease (CHD). It turns out that the significance of perfusion parameters strongly depends on the individual patient, scanning parameters, and data pre-processing.
Subject(s)
Blood Flow Velocity/physiology , Blood Vessels/physiology , Computer Graphics , Models, Cardiovascular , Rheology/methods , User-Computer Interface , Computer Simulation , Databases, Factual , HumansABSTRACT
This paper presents a scalable framework for real-time raycasting of large unstructured volumes that employs a hybrid bricking approach. It adaptively combines original unstructured bricks in important (focus) regions, with structured bricks that are resampled on demand in less important (context) regions. The basis of this focus+context approach is interactive specification of a scalar degree of interest (DOI) function. Thus, rendering always considers two volumes simultaneously: a scalar data volume, and the current DOI volume. The crucial problem of visibility sorting is solved by raycasting individual bricks and compositing in visibility order from front to back. In order to minimize visual errors at the grid boundary, it is always rendered accurately, even for resampled bricks. A variety of different rendering modes can be combined, including contour enhancement. A very important property of our approach is that it supports a variety of cell types natively, i.e., it is not constrained to tetrahedral grids, even when interpolation within cells is used. Moreover, our framework can handle multi-variate data, e.g., multiple scalar channels such as temperature or pressure, as well as time-dependent data. The combination of unstructured and structured bricks with different quality characteristics such as the type of interpolation or resampling resolution in conjunction with custom texture memory management yields a very scalable system.