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1.
Int J Mol Sci ; 24(7)2023 Apr 05.
Article in English | MEDLINE | ID: mdl-37047735

ABSTRACT

Plant surface properties are crucial determinants of resilience to abiotic and biotic stresses. The outer layer of the plant cuticle consists of chemically diverse epicuticular waxes. The WAX INDUCER1/SHINE subfamily of APETALA2/ETHYLENE RESPONSIVE FACTORS regulates cuticle properties in plants. In this study, four barley genes homologous to the Arabidopsis thaliana AtWIN1 gene were mutated using RNA-guided Cas9 endonuclease. Mutations in one of them, the HvWIN1 gene, caused a recessive glossy sheath phenotype associated with ß-diketone deficiency. A complementation test for win1 knockout (KO) and cer-x mutants showed that Cer-X and WIN1 are allelic variants of the same genomic locus. A comparison of the transcriptome from leaf sheaths of win1 KO and wild-type plants revealed a specific and strong downregulation of a large gene cluster residing at the previously known Cer-cqu locus. Our findings allowed us to postulate that the WIN1 transcription factor in barley is a master mediator of the ß-diketone biosynthesis pathway acting through developmental stage- and organ-specific transactivation of the Cer-cqu gene cluster.


Subject(s)
Arabidopsis , Hordeum , Hordeum/genetics , Hordeum/metabolism , Waxes/metabolism , Transcription Factors/metabolism , Arabidopsis/genetics , Arabidopsis/metabolism , Plant Leaves/metabolism , Multigene Family , Gene Expression Regulation, Plant , Plant Epidermis/genetics
2.
Chem Biodivers ; 19(2): e202100755, 2022 Feb.
Article in English | MEDLINE | ID: mdl-34918866

ABSTRACT

Conifer essential oils have been used for centuries in traditional medicine, and nowadays they are of special interest for official medicine, aromatherapy and perfumery. In the present work, comprehensive information is given on the composition of essential oils prepared from the twigs of the conifer trees of the pine family (Pinaceae): Abies sibirica Ledeb., Larix sibirica Ledeb., Picea obovata Ledeb., Pinus sibirica Du Tour, Pinus sylvestris L. A total of 50 samples of essential oils have been studied. The samples were prepared during vegetation stage in the time period 1998-2012 from the growing wild trees in the South part of the Western Siberia (Russian Federation) and neighboring territories of Republic of Kazakhstan within the area with geographical coordinates LAT 49.180012-57.908583 and LON 83.213217-91.258717 at elevation of 82-2070 m above sea level. All the essential oil samples were obtained from freshly collected plant raw material by steam distillation at atmospheric pressure in stainless steel apparatus, which had been specially designed for field research. All the chromatographic profiles were prepared from authentic samples whose voucher specimens are deposited at the Central Siberian Botanical Garden of the Siberian Branch of the Russian Academy of Sciences (NS). The following information for each sample is provided: (1) date and location of the plant raw material collecting, indicating administrative areas and the exact geographic coordinates; (2) yield of essential oil, (3) chemical composition of the essential oil sample based on GC/MS experiments using full mass-spectra (EI, 70 eV) and linear retention indices of the components, (4) results of GC-FID quantification based on internal standards and response factors, (5) enantiomeric composition of the main components based on GC×GC experiments using the 2nd column with cyclodextrin-based chiral selector, (6) GC profile of the high-boiling fractions indicating the characteristic sesquiterpenoids. Therefore, this study provides reliable information about the variability and true composition of the Siberian conifer oils, and the experimental data given can serve as reference chromatographic profiles of volatile substances to solve the problems of quality, authenticity and safety.


Subject(s)
Oils, Volatile , Pinaceae , Tracheophyta , Oils, Volatile/chemistry , Plant Oils , Quality Control , Trees
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