ABSTRACT
Fifty patients with stable angina who had undergone elective coronary angioplasty with stenting were examined. Myocardial microlesions diagnosed from the elevated levels of troponin (Tn) I and creatine phosphate kinase MB (CPK-MB) were detected in 48-54% of the patients; however, the content of Tn I exceeded the high reference level only in 18%. Tn I is a more specific method than CPK-MB in detecting myocardial microlesions during intracoronary intervention. The signs of myocardial microlesions were most commonly detected during intervention into the arteries with types B and C stenoses in case of complicated or technically difficult stent implantation.
Subject(s)
Angioplasty, Balloon, Coronary/adverse effects , Myocardium/pathology , Stents , Adult , Aged , Aged, 80 and over , Coronary Stenosis/pathology , Coronary Stenosis/therapy , Creatine Kinase/blood , Female , Humans , Male , Middle Aged , Necrosis , Troponin I/bloodABSTRACT
Using the method of peroxidative luminol-dependent chemiluminescence we showed that combined treatment with aspirin in low dose and dipyridamole corrects imbalance in oxidative homeostasis in mouse serum. This state resulted from a sharp increase in prooxidant processes after platelet disaggregation with normal dose of aspirin or dose-dependent inhibition of free radical processes with dipyridamole.
Subject(s)
Aspirin/pharmacology , Blood/metabolism , Dipyridamole/pharmacology , Homeostasis/drug effects , Platelet Aggregation Inhibitors/pharmacology , Animals , Antioxidants/metabolism , Blood/drug effects , Dose-Response Relationship, Drug , Free Radicals/metabolism , Luminescent Measurements , Mice , Mice, Inbred C57BL , Oxidation-Reduction , Platelet Aggregation/drug effectsABSTRACT
Depending on the type of autonomous regulation, differences in basic levels of interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF alpha) were revealed under conditions of hyperthermia in healthy subjects aged 19-21. A parasympathetic type of autonomous regulation corresponded to higher initial levels of proinflammatory cytokinesis, whereas a dominating sympathetic type corresponded to lower levels of the IL-1 beta and TNF alpha. The subjects with the latter type of regulation revealed an increase in the IL-1 beta TNF alpha combined with a higher heat tolerance. The subjects with the former type of regulation revealed a lower heat tolerance. The increase in the alpha2-macroglobulin appeared to be a most typical acute phase response of the human body to hyperthermia.
Subject(s)
Acute-Phase Reaction , Autonomic Nervous System/physiology , Hot Temperature , Acute-Phase Proteins/biosynthesis , Adult , Blood Pressure , Body Temperature , Exercise Test , Heart Rate , Humans , Interleukin-1/biosynthesis , Male , Parasympathetic Nervous System/physiology , Sympathetic Nervous System/physiology , Tumor Necrosis Factor-alpha/biosynthesisABSTRACT
The structure and biochemical properties of the major soluble regulatory proteins of the complement, including the CI-inhibitor, factors I, H, and J, properdin, the C4-binding protein and other protein components, are reviewed. Three types of the proteolytic complement cascade regulation by proteins are envisaged: the first one involves the inhibition of complement proteases, the second one is based on proteolytic degradation of complement activation products and the third one includes the regulation of stability of complement protein complexes. The main functional role of humoral regulatory proteins consists in the limitation of the proteolytic cascade.
Subject(s)
Complement Activation , Proteins/physiology , Complement System Proteins/physiology , Humans , Hydrolysis , Protease InhibitorsABSTRACT
A new procedure was developed for estimating the esterase activity of proteolytic enzymes by using CIS subcomponent of complement and N-alpha-benzoyl-L-arginine ethyl ester as a substrate. In contrast to other methods, which used static variants of gas chromatographic vapour-phase analysis for registration of the volatile reaction product alcohol, the procedure involved steady gas extraction of the volatile product in the gas stream over the incubation mixture. This method enabled to obtain a kinetic curve during one experiment as well as to elevate the accuracy of enzymatic activity estimation.
