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1.
Development ; 151(8)2024 Apr 15.
Article in English | MEDLINE | ID: mdl-38619323

ABSTRACT

Regulation of chromatin states is essential for proper temporal and spatial gene expression. Chromatin states are modulated by remodeling complexes composed of components that have enzymatic activities. CHD4 is the catalytic core of the nucleosome remodeling and deacetylase (NuRD) complex, which represses gene transcription. However, it remains to be determined how CHD4, a ubiquitous enzyme that remodels chromatin structure, functions in cardiomyocytes to maintain heart development. In particular, whether other proteins besides the NuRD components interact with CHD4 in the heart is controversial. Using quantitative proteomics, we identified that CHD4 interacts with SMYD1, a striated muscle-restricted histone methyltransferase that is essential for cardiomyocyte differentiation and cardiac morphogenesis. Comprehensive transcriptomic and chromatin accessibility studies of Smyd1 and Chd4 null embryonic mouse hearts revealed that SMYD1 and CHD4 repress a group of common genes and pathways involved in glycolysis, response to hypoxia, and angiogenesis. Our study reveals a mechanism by which CHD4 functions during heart development, and a previously uncharacterized mechanism regarding how SMYD1 represses cardiac transcription in the developing heart.


Subject(s)
DNA Helicases , DNA-Binding Proteins , Gene Expression Regulation, Developmental , Heart , Mi-2 Nucleosome Remodeling and Deacetylase Complex , Myocytes, Cardiac , Transcription Factors , Animals , Humans , Mice , Cell Differentiation/genetics , Chromatin/metabolism , Glycolysis/genetics , Heart/embryology , Histone-Lysine N-Methyltransferase/metabolism , Histone-Lysine N-Methyltransferase/genetics , Mi-2 Nucleosome Remodeling and Deacetylase Complex/metabolism , Mi-2 Nucleosome Remodeling and Deacetylase Complex/genetics , Mice, Knockout , Muscle Proteins/metabolism , Muscle Proteins/genetics , Myocytes, Cardiac/metabolism , Proteomics , Transcription, Genetic
2.
Dev Cell ; 56(21): 3019-3034.e7, 2021 11 08.
Article in English | MEDLINE | ID: mdl-34655525

ABSTRACT

Sex disparities in cardiac homeostasis and heart disease are well documented, with differences attributed to actions of sex hormones. However, studies have indicated sex chromosomes act outside of the gonads to function without mediation by gonadal hormones. Here, we performed transcriptional and proteomics profiling to define differences between male and female mouse hearts. We demonstrate, contrary to current dogma, cardiac sex disparities are controlled not only by sex hormones but also through a sex-chromosome mechanism. Using Turner syndrome (XO) and Klinefelter (XXY) models, we find the sex-chromosome pathway is established by X-linked gene dosage. We demonstrate cardiac sex disparities occur at the earliest stages of heart formation, a period before gonad formation. Using these datasets, we identify and define a role for alpha-1B-glycoprotein (A1BG), showing loss of A1BG leads to cardiac defects in females, but not males. These studies provide resources for studying sex-biased cardiac disease states.


Subject(s)
Gonads/growth & development , Gonads/metabolism , Proteomics , Sex Characteristics , Sex Chromosomes/metabolism , Animals , Female , Genes, X-Linked/genetics , Male , Mice , Proteomics/methods
3.
Curr Opin Chem Biol ; 48: 150-157, 2019 02.
Article in English | MEDLINE | ID: mdl-30711722

ABSTRACT

Congenital malformations, or structural birth defects, are now the leading cause of infant mortality in the United States and Europe (Dolk et al., 2010; Heron et al., 2009). Of the congenital malformations, congenital heart disease (CHD) is the most common (Dolk et al., 2010; Heron et al., 2009). Thus, a molecular understanding of heart development is an essential goal for improving clinical approaches to CHD. However, CHDs are commonly a result of genetic defects that manifest themselves in a spatial and temporal manner during the early stages of embryogenesis, leaving them mostly intractable to mass spectrometry-based analysis. Here, we describe the technologies and advancements in the field of mass spectrometry over the past few years that have begun to provide insights into the molecular and cellular basis of CHD and prospects for these types of approaches in the future.


Subject(s)
Heart Defects, Congenital/etiology , Heart/embryology , Mass Spectrometry/methods , Proteomics/methods , Animals , Cell Differentiation , Cellular Reprogramming , Embryonic Stem Cells/cytology , Fibroblasts/cytology , Humans , Induced Pluripotent Stem Cells/cytology , Myocytes, Cardiac/cytology , Proteome/analysis
4.
Dev Cell ; 36(3): 262-75, 2016 Feb 08.
Article in English | MEDLINE | ID: mdl-26859351

ABSTRACT

Human mutations in the cardiac transcription factor gene TBX5 cause congenital heart disease (CHD), although the underlying mechanism is unknown. We report characterization of the endogenous TBX5 cardiac interactome and demonstrate that TBX5, long considered a transcriptional activator, interacts biochemically and genetically with the nucleosome remodeling and deacetylase (NuRD) repressor complex. Incompatible gene programs are repressed by TBX5 in the developing heart. CHD mis-sense mutations that disrupt the TBX5-NuRD interaction cause depression of a subset of repressed genes. Furthermore, the TBX5-NuRD interaction is required for heart development. Phylogenetic analysis showed that the TBX5-NuRD interaction domain evolved during early diversification of vertebrates, simultaneous with the evolution of cardiac septation. Collectively, this work defines a TBX5-NuRD interaction essential to cardiac development and the evolution of the mammalian heart, and when altered may contribute to human CHD.


Subject(s)
Chromatin Assembly and Disassembly/genetics , Gene Expression Regulation, Developmental/genetics , Heart/embryology , Myocardium/metabolism , T-Box Domain Proteins/genetics , Animals , Humans , Mice, Transgenic , Organogenesis/genetics , Organogenesis/physiology , T-Box Domain Proteins/metabolism , Transcription, Genetic/genetics
5.
Development ; 142(11): 2037-47, 2015 Jun 01.
Article in English | MEDLINE | ID: mdl-25953344

ABSTRACT

Organ growth occurs through the integration of external growth signals during the G1 phase of the cell cycle to initiate DNA replication. Although numerous growth factor signals have been shown to be required for the proliferation of cardiomyocytes, genetic studies have only identified a very limited number of transcription factors that act to regulate the entry of cardiomyocytes into S phase. Here, we report that the cardiac para-zinc-finger protein CASZ1 is expressed in murine cardiomyocytes. Genetic fate mapping with an inducible Casz1 allele demonstrates that CASZ1-expressing cells give rise to cardiomyocytes in the first and second heart fields. We show through the generation of a cardiac conditional null mutation that Casz1 is essential for the proliferation of cardiomyocytes in both heart fields and that loss of Casz1 leads to a decrease in cardiomyocyte cell number. We further report that the loss of Casz1 leads to a prolonged or arrested S phase, a decrease in DNA synthesis, an increase in phospho-RB and a concomitant decrease in the cardiac mitotic index. Taken together, these studies establish a role for CASZ1 in mammalian cardiomyocyte cell cycle progression in both the first and second heart fields.


Subject(s)
DNA-Binding Proteins/metabolism , G1 Phase , Heart/embryology , Mammals/embryology , Myocytes, Cardiac/cytology , Myocytes, Cardiac/metabolism , S Phase , Transcription Factors/metabolism , Animals , Cell Lineage , Cell Proliferation , Embryo, Mammalian/metabolism , Female , Integrases/metabolism , Male , Mice , Myocardium/cytology , Myocardium/metabolism , Myocardium/ultrastructure
7.
Dev Cell ; 25(2): 132-43, 2013 Apr 29.
Article in English | MEDLINE | ID: mdl-23639441

ABSTRACT

The formation of the vascular system is essential for embryonic development and homeostasis. However, transcriptional control of this process is not fully understood. Here we report an evolutionarily conserved role for the transcription factor CASZ1 (CASTOR) in blood vessel assembly and morphogenesis. In the absence of CASZ1, Xenopus embryos fail to develop a branched and lumenized vascular system, and CASZ1-depleted human endothelial cells display dramatic alterations in adhesion, morphology, and sprouting. Mechanistically, we show that CASZ1 directly regulates Epidermal Growth Factor-Like Domain 7 (Egfl7). We further demonstrate that defects of CASZ1- or EGFL7-depleted cells are in part due to diminished RhoA expression and impaired focal adhesion localization. Moreover, these abnormal endothelial cell behaviors in CASZ1-depleted cells can be rescued by restoration of Egfl7. Collectively, these studies show that CASZ1 is required to directly regulate an EGFL7/RhoA-mediated pathway to promote vertebrate vascular development.


Subject(s)
DNA-Binding Proteins/metabolism , Embryo, Nonmammalian/metabolism , Endothelial Growth Factors/metabolism , Endothelium, Vascular/cytology , Extracellular Matrix Proteins/metabolism , Gene Expression Regulation , Morphogenesis/physiology , Transcription Factors/metabolism , Xenopus Proteins/metabolism , Xenopus laevis/metabolism , rhoA GTP-Binding Protein/metabolism , Animals , Blotting, Western , Calcium-Binding Proteins , Cell Adhesion , Cell Differentiation , Cell Proliferation , Cells, Cultured , Chromatin Immunoprecipitation , DNA-Binding Proteins/genetics , EGF Family of Proteins , Embryo, Nonmammalian/cytology , Embryonic Development , Endothelial Growth Factors/genetics , Endothelium, Vascular/metabolism , Extracellular Matrix Proteins/genetics , Female , Fluorescent Antibody Technique , Human Umbilical Vein Endothelial Cells/cytology , Human Umbilical Vein Endothelial Cells/metabolism , Humans , Immunoenzyme Techniques , Molecular Sequence Data , Transcription Factors/genetics , Transcription, Genetic , Xenopus Proteins/genetics , Xenopus laevis/growth & development , rhoA GTP-Binding Protein/genetics
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