ABSTRACT
Chemical bonding reaction and immobilization through low energy radiation (heating) have been investigated to fix a side-chain liquid crystalline polymer (SC-LCP) on silica particles in order to use the resulting modified silica in normal-phase HPLC. Highly stable chromatographic stationary phases are observed under excellent polymer solvent flow conditions (THF) for both methods and better column efficiencies are also exhibited towards PAHs' separation compared to the classical coated stationary phase. The characterization of these new stationary phases and the rationale for improved column stability have been investigated by solid state 13C and 29Si CP/MAS NMR spectroscopy. It is clearly shown that the chemical bonding is achieved by the classical hydrosilylation reaction between PHMS chains and vinyl modified silica. The bonded polymer is likely a copolymer than a homopolymer. The immobilization of the SC-LCP by heating results in the breaking of Si-O-Si bonds of the polysiloxane chain after the attack of the silica surface silanols. Applications to fullerenes and carotenes separation of these bonded stationary phases are compared to the separation power of a classical monomeric C18 stationary phase in NP-HPLC as n-hexane-toluene or methyl-tertiobutyl ether-methanol mixtures.
Subject(s)
Magnetic Resonance Spectroscopy/methods , Polymers/chemistry , Silicon Dioxide/chemistry , Crystallization , Polycyclic Compounds/isolation & purificationABSTRACT
The 35-residue peptide corresponding to the very hydrophobic transmembrane region of the tyrosine kinase receptor neu, Neu(TM35), has been synthesized. The peptide can be solubilized in millimolar concentrations in TFE or incorporated into an SDS-water micellar solution or into well-hydrated DMPC/DCPC bicelles. In all these media, circular dichroism demonstrated that the peptide adopts a helical structure for about 80% of its amino acids. The peptide is monomeric below 2 mM in TFE, as also determined by variable concentration experiments. The three-dimensional solution structure in TFE has been obtained by homonuclear proton NMR and shows a well-defined alpha-helix from residues 4 to 21, then a pi-bulge from Ile(22) to Gly(28), and a final short alpha-helix from positions 29 to 32. This experimental finding is in agreement with structures predicted recently by molecular dynamics calculations in a vacuum [Sajot, N., and Genest, M. (2000) Eur. Biophys. J. 28, 648-662]. The biological implications of a possible retention of this structure in a membrane environment are finally discussed.
Subject(s)
Membrane Proteins/chemistry , Peptide Fragments/chemistry , Receptor, ErbB-2/chemistry , Amino Acid Sequence , Circular Dichroism , Dimyristoylphosphatidylcholine/chemistry , Lipid Bilayers/chemistry , Membrane Proteins/chemical synthesis , Micelles , Molecular Sequence Data , Nuclear Magnetic Resonance, Biomolecular/methods , Peptide Fragments/chemical synthesis , Phospholipid Ethers/chemistry , Protein Structure, Secondary , Sodium Dodecyl Sulfate , Solutions , Thermodynamics , Trifluoroethanol/chemistryABSTRACT
The influence of melittin on two DMPA membrane systems at pH 4.2 and 8.2 has been investigated by solid-state 31P and 2H NMR, as a function of temperature and peptide concentration. Melittin promotes greater morphological changes for both systems in the fluid phase, the effect being larger at pH 4.2. Close inspection of fatty acyl chain dynamics suggests that some parallels can be drawn between the DMPA/melittin at pH 8.2 and PC/melittin systems. In addition, at pH 8.2 a direct neutralization at the interface of one of the lipid negative charges by a positive charge of the peptide occurs, as can be monitored by 31P NMR at the molecular level. For the system at pH 4.2 and at high temperature, a lipid-to-peptide molar ratio of 30 is sufficient to transform the whole system into an isotropic phase, proposed to be inverted micelles. When the system is cooled down towards the gel phase one observes an intermediate hexagonal phase in a narrow range of temperature.
Subject(s)
Aniline Compounds/chemistry , Lipid Bilayers/chemistry , Melitten/chemistry , Hydrogen-Ion Concentration , Magnetic Resonance Spectroscopy , Phosphorus Isotopes , X-Ray DiffractionABSTRACT
High quality purification of membrane-spanning peptides and proteins remains a challenging problem. In this work we describe a tailored chromatographic purification of a synthetic 35-residue peptide corresponding to the transmembrane region of the tyrosine kinase receptor c-erb2/neu. Composed to over 70% by the amino acids alanine, isoleucine, leucine, phenylalanine and valine, this peptide presents a very hydrophobic character. Product isolation from the complex peptide mixture, obtained after acid cleavage of the resin matrix used during the solid-phase synthesis, represents a difficult task. We propose a three step strategy based on gel permeation and reversed-phase high-performance liquid chromatography, using aprotic polar solvent mixtures. The challenge consisted in obtaining a sufficient amount of an extremely pure sample, in order to allow structural analysis by NMR spectroscopy. Keeping trace of the synthetic peptide throughout the different purification steps was assured by MALDI TOF mass spectrometry, and the final product purity was checked by coupled liquid chromatography-ESI TOF mass spectrometry.
Subject(s)
Membrane Proteins/chemistry , Peptide Fragments/isolation & purification , Receptor, ErbB-2/chemistry , Amino Acid Sequence , Magnetic Resonance Spectroscopy , Molecular Sequence Data , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
Chemical synthesis of highly hydrophobic peptides and proteins remains a challenging problem. Strong interchain associations within the peptide-resin matrix have to be overcome. A synthetic strategy for solid phase peptide synthesis is proposed, mainly based on prolonged coupling time using aprotic polar solvent mixtures. A tailored chromatographic purification was required to obtain a sample sufficiently pure for structural analysis. In this work, the total chemical synthesis of the membrane-embedded yeast mitochondrial ATP synthase subunit 8 is described. The quality of the synthetic protein was checked by electrospray mass spectrometry, its tendency to adopt alpha-helical secondary structure is evidenced by circular dichroism spectroscopy.
Subject(s)
Intracellular Membranes/enzymology , Mitochondria/ultrastructure , Proton-Translocating ATPases/chemical synthesis , Amino Acid Sequence , Chromatography, High Pressure Liquid , Circular Dichroism , Membrane Proteins/chemical synthesis , Protein Structure, Secondary , Saccharomyces cerevisiae/ultrastructure , Yeasts/ultrastructureABSTRACT
Proton decoupled deuterium NMR spectra of oriented bilayers made of DMPC and 30 mol % deuterated cholesterol acquired at 76.8 MHz (30 degreesC) have provided a set of very accurate quadrupolar splitting for eight C-D bonds of cholesterol. Due to the new precision of the experimental data, the original analysis by. Biochemistry. 23:6062-6071) had to be reconsidered. We performed a systematic study of the influence on the precision and uniqueness of the data-fitting procedure of: (i) the coordinates derived from x-ray, neutron scattering, or force field-minimized structures, (ii) internal mobility, (iii) the axial symmetry hypothesis, and (iv) the knowledge of some quadrupolar splitting assignments. Good agreement between experiment and theory could be obtained only with the neutron scattering structure, for which both axial symmetry hypothesis and full order parameter matrix analysis gave satisfactory results. Finally, this work revealed an average orientation of cholesterol slightly different from those previously published and, most importantly, a molecular order parameter equal to 0.95 +/- 0.01, instead of 0.79 +/- 0.03 previously found for the same system at 30 degreesC. Temperature dependence in the 20-50 degreesC range shows a constant average orientation and a monotonous decrease of cholesterol Smol, with a slope of -0.0016 K-1. A molecular order parameter of 0.89 +/- 0.01 at 30 degreesC was determined for a DMPC/16 mol % of cholesterol.
Subject(s)
Cholesterol/chemistry , Dimyristoylphosphatidylcholine/chemistry , Lipid Bilayers/chemistry , Biophysical Phenomena , Biophysics , Deuterium , Magnetic Resonance Spectroscopy , Molecular Structure , ThermodynamicsABSTRACT
Cadmium(II) interactions with multilamellar vesicles of dimyristoyl (DM)- and dipalmitoyl (DP)-phosphatidylcholine (PC), -phosphatidylserine (PS), -phosphatidic acid (PA), -phosphatidylglycerol (PG) and -phosphatidylethanolamine (PE) have been investigated both from the metal and the membrane viewpoints, respectively, by solution 113Cd-NMR and diphenylhexatriene fluorescence polarization coupled with solid-state 31P-NMR. Results can be summarized as follows. (1) Strong cadmium binding to membrane phospholipids results in a decrease of the free Cd(II) 113Cd-NMR isotropic signal and because of slow exchange, in the NMR time scale, between free and bound cadmium pools, the lipid/water partition coefficients, Klw, of the Cd(II) species can be determined in the lamellar gel (fluid) phase. It is found Klw(DMPC) approximately Klw(EggPE) approximately 2+/-2 (2+/-2); Klw(DMPA)=392+/-20 (505+/-25); Klw(DMPG)=428+/-21 (352+/-17); Klw(DMPS)=544+/-27 (672+/-34). Cadmium interactions with membrane phospholipids are therefore electrostatic in nature and the phosphate moiety is proposed as a potential binding site. (2) The presence of Cd(II) stabilizes the gel phases of PG, PA and PS lipids and leads to suppression of the main phase transition for PA and PS. These effects are reduced upon increasing salinity to 0.5 M Cl- and abolished at 1.8 M Cl-, Cd(II) being removed from the membranes due to formation of soluble CdCln species. Moving the pH from 7 to 6 also decreases Cd(II) binding to PA, because of surface charge reduction. (3) Cadmium promotes the formation of isotropic 31P-NMR lines with PG systems and of a hexagonal phase on egg PE bilayers at 24 degreesC, suggesting dramatic membrane reorganization. Properties of cadmium and calcium interacting with phospholipid model membranes are compared, and the potential roles of these interactions in the molecular mechanisms of cadmium uptake and toxicity in cells are discussed.
Subject(s)
Cadmium/chemistry , Lipid Bilayers/chemistry , Phospholipids/chemistry , Cadmium/toxicity , Cadmium Chloride/chemistry , Fluorescence Polarization , Hydrogen-Ion Concentration , Isotopes , Magnetic Resonance Spectroscopy , Sodium Chloride , TemperatureABSTRACT
To investigate whether lipid solubilization is of relevance in describing the interaction between melittin and biological membranes, we studied melittin-induced polymorphism using model membranes composed of the biological lipid sphingomyelin (bovine brain). The behavior of the system was monitored by solid state 31P-NMR and turbidity measurements and compared to the peptides well-characterized action on the synthetic lipid dipalmitoylphosphatidylcholine. It was found that melittin-induced macroscopic changes of sphingomyelin membranes are qualitatively the same as in the case of dipalmitoyl-phosphatidylcholine bilayers. The sphingomyelin/melittin system is thus proposed to show a reversible vesicle-to-disc transition (fluid-to-gel phase) through an intermediate fusion or aggregation event centered at the main transition temperature, Tm, as reported in the case of saturated phosphatidylcholine. In the case of spontaneous disc formation at 37 degrees C, the lipid-to-peptide molar ratio in the discoidal objects was determined to be approximately 20 for dipalmitoylphosphatidylcholine and about 12 in the case of natural sphingomyelin. Melittin partition coefficients between membranes and the aqueous medium at 37 degrees C were found to be 6.1 +/- 0.8 mM-1 and 3.7 +/- 0.4 mM-1 for sphingomyelin and dipalmitoylphosphatidylcholine, respectively. For very high peptide quantities (lipid-to-peptide molar ratio, Ri < or = 5) mixed micelles are formed over the entire temperature range (20 degrees to 60 degrees C) for both kinds of lipids.
Subject(s)
Lipid Bilayers/chemistry , Melitten/chemistry , Melitten/pharmacology , Phosphatidylcholines/chemistry , Sphingomyelins/chemistry , Animals , Brain/drug effects , Brain/metabolism , Brain/ultrastructure , Cattle , Cell Membrane/drug effects , Cell Membrane/metabolism , Kinetics , Magnetic Resonance Spectroscopy/methods , Micelles , Nephelometry and Turbidimetry , Solutions , TemperatureABSTRACT
31P two-dimensional exchange solid-state NMR spectroscopy was used to measure the lateral diffusion, D(L), in the fluid phase of dipalmitoylphosphatidylcholine (DPPC) in the presence and absence of melittin. The use of a spherical solid support with a radius of 320 +/- 20 nm, on which lipids and peptides are adsorbed together, and a novel way of analyzing the two-dimensional exchange patterns afforded a narrow distribution of D(L) centered at a value of (8.8 +/- 0.5) x 10(-8) cm2/s for the pure lipid system and a large distribution of D(L) spanning 1 x 10(-8) to 10 x 10(-8) cm2/s for the lipids in the presence of melittin. In addition, the determination of D(L) for nonsupported DPPC multilamellar vesicles (MLVs) suggests that the support does not slow down the lipid diffusion and that the radii of the bilayers vary from 300 to 800 nm. Finally, the DPPC-melittin complex is stabilized at the surface of the silica beads in the gel phase, opening the way to further study of the interaction between melittin and DPPC.
Subject(s)
1,2-Dipalmitoylphosphatidylcholine/chemistry , Liposomes , Melitten , Diffusion , Kinetics , Models, Theoretical , Nuclear Magnetic Resonance, Biomolecular , Phosphorus , Silicon Dioxide , Spectroscopy, Fourier Transform InfraredABSTRACT
Interaction of nystatin A1 with multilamellar vesicles (MLV) of dilauroylphosphatidylcholine (DLPC), observed either by adding nystatin to preformed MLV (mixtures I) or by incorporating it during the formation of vesicles (mixtures II, inner lamellas of MLV in contact with nystatin) was investigated for 0.002 < or = nystatin/DLPC = R(A) < or = 0.20, by four complementary methods. The main results were: (i) Ultraviolet absorption and circular dichroism (CD) spectra of mixtures I revealed the occurrence of a saturable association with a stoichiometry (R(A) = 0.007 +/- 0.002) constant between 3 and 33 degrees C. (ii) By differential scanning calorimetry, thermograms of the two types of mixtures were similar only when water was in great excess. In the opposite (e.g., (H2O)/(DLPC) = R(W) < or = 300), mixture II thermograms displayed two features, upshifted by about 6.5 degrees C with respect to the sharp peak observed with mixture I, resembling those obtained for pure DLPC when the low-temperature phase was the subgel phase. For this R(W), the nystatin absolute concentrations were those for which nystatin form superaggregates as revealed by the nystatin CD spectra. It is proposed that these superaggregates are excluded from the interlamellar spacings of MLV and exert a pumping action on the interlamellar water. The subsequent dehydration of the inner lamellas is thought to convert them into the subgel state. (iii) 2H-NMR spectra of sn-2-perdeuterated DLPC MLV + nystatin mixtures II, confirmed such a temperature shift of the main transition. They showed, in addition, an ordering of the aliphatic chains immediately above the transition temperature, equivalent to a bilayer thickening of 2 A.
Subject(s)
Anti-Bacterial Agents/chemistry , Lipid Bilayers/chemistry , Nystatin/chemistry , Phosphatidylcholines/chemistry , Polyenes/chemistry , Calorimetry, Differential Scanning , Circular Dichroism , Magnetic Resonance SpectroscopyABSTRACT
The number of water molecules bound to the dimyristoylphosphatidylcholine (DMPC) interface was investigated both in the fluid (L(alpha)) and gel (L(beta')) phases by solid state deuterium NMR of D2O. We determined that each DMPC molecule binds 9.7 +/- 0.5 and less than 4.3 +/- 0.5 D2O in the fluid and gel phases respectively. These results are accounted for by considering the number of DMPC binding sites as well as the molecular organization in each phase.
Subject(s)
Dimyristoylphosphatidylcholine/chemistry , Calorimetry, Differential Scanning , Deuterium , Gels , Magnetic Resonance Spectroscopy , Water/chemistryABSTRACT
The ordering of 30 mol % cholesterol (CH) or cholesterol sulfate (CS) on chain deuterated dimyristoylphosphatidylcholine (DMPC) was investigated by 2H-NMR for different hydrations. It is found that: (i) hydration has merely no influence on chain order (chain length) for DMPC-cholesterol systems, (ii) in CS-containing mixtures chain order (length) is greater at low hydration (DMPC-to-water molar ratio, Ri, of 11.3) than in excess water (Ri approximately 500) and (iii) at low hydration the ordering is about the same for CS or CH-containing systems whereas it is not at high hydration. DMPC-CS bilayer thickness is therefore very sensitive to hydration.
Subject(s)
Cholesterol Esters , Dimyristoylphosphatidylcholine , Membranes, Artificial , Hydrogen Bonding , Magnetic Resonance SpectroscopyABSTRACT
The comparative effect of cholesterol (CH) versus cholesterol sulfate (CS) on dimyristoylphosphatidylcholine (DMPC) membranes has been investigated by optical microscopy, freeze-fracture electron microscopy, x-ray diffraction, and solid state 2H and 31P nuclear magnetic resonance (NMR). The sulfate analogue extends the lamellar phase domain toward high water contents, and substitution of 30 mol % CH by CS in DMPC lamellae induces the trapping of 30 wt % additional water. The greater swelling of the CS-containing systems is evidenced by determination of lamellar repeat distances at maximal hydration: 147 +/- 4 A and 64 +/- 2 A in the presence of CS and CH, respectively. 2H-NMR of heavy water demonstrates that CS binds approximately 12 more water molecules at the interface than CH whereas NMR of deuterium-labeled DMPC chains reveals that 30 mol % CS orders the membrane as 15 mol % CH at high temperature and disorders much more than CH at low temperatures. The various effects of CS versus CH are discussed by taking into account attractive Van der Waals forces and repulsive steric/electrostatic interactions of the negatively charged sulfate group.
Subject(s)
Cholesterol Esters/chemistry , Cholesterol/chemistry , Dimyristoylphosphatidylcholine/chemistry , Membrane Lipids/chemistry , Binding Sites , Biophysical Phenomena , Biophysics , Deuterium Oxide , In Vitro Techniques , Lipid Bilayers/chemistry , Magnetic Resonance Spectroscopy , Models, Chemical , Thermodynamics , Water/chemistry , X-Ray DiffractionABSTRACT
The binding of inorganic mercury Hg(II) to phospholipid headgroups has been investigated by phosphorus-31 nuclear magnetic resonance of phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylcholine (PC) in water micellar and multilamellar phases. HgCl2 triggers the aggregation of phospholipid micelles, leading to a lipid-mercury precipitate that is no longer detectable by high-resolution 31P-NMR. The remaining signal area corresponds to micelles in the soluble fraction and is a non-linear function of the initial mercury-to-lipid molar ratio. Kinetics of micelle aggregation are exponential for the first 15 min and show a plateau tendency after 120 min. Apparent Hg(II) affinities for phospholipid headgroups are in the order: PE > PS > PC. The same binding specificity is observed when HgCl2 is added to (1:1) mixtures of different micelles (PE + PC; PS + PC). However, mercury binding to mixed micelles prepared with two lipids (PE/PC or PS/PC) induces the aggregation of both lipids. Hg(II) also leads to a 31P-NMR chemical shift anisotropy decrease of PC, PS and mixed (1:1) PE/PC multilamellar vesicles and markedly broadens PS spectra. This indicates that HgCl2 binding forces phospholipid headgroups to reorient and that the concomitant network formation leads to a slowing down of PS membrane collective motions. Formation of a gel-like lamellar phase characterized by a broad NMR linewidth is also observed upon HgCl2 binding to PE samples both in fluid (L alpha) or hexagonal (H(II)) phases. The PE hexagonal phase is no longer detected in the presence of HgCl2. Mixed PE/PC dispersions remain in the fluid phase upon mercury addition, indicating that no phase separation occurs. Addition of excess NaCl leads to the appearance of the non-reactive species HgCl4(2-) and induces the reversal of all the above effects.
Subject(s)
Membranes, Artificial , Mercury/chemistry , Phospholipids/chemistry , Binding Sites , Biophysical Phenomena , Biophysics , Kinetics , Magnetic Resonance Spectroscopy , Micelles , Phosphatidylcholines/chemistry , Phosphatidylethanolamines/chemistry , Phosphatidylserines/chemistry , Phosphorus/chemistryABSTRACT
The effect of pH and cholesterol on the dimyristoylphosphatidic acid (DMPA) model membrane system has been investigated by solid state 2H- and 31P-NMR. It has been shown that each of the three protonation states of the DMPA molecule corresponds to a 31P-NMR powder pattern with characteristic delta sigma values; this implies additionally that the proton exchange on the membrane surface is slow on the NMR time scale (millisecond range). Under these conditions, the 2H-labeled lipid chains sense only one magnetic environment, indicating that the three spectra detected by 31P-NMR are related to charge-dependent local dynamics or orientations of the phosphate headgroup or both. Chain ordering in the fluid phase is also found to depend weakly on the charge at the interface. In addition, it has also been found that the first pK of the DMPA membrane is modified by changes in the lipid lateral packing (gel or fluid phases or in the presence of cholesterol) in contrast to the second pK. The incorporation of 30 mol% cholesterol affects the phosphatidic acid bilayer in a way similar to what has been reported for phosphatidylcholine/cholesterol membranes, but to an extent comparable to 10-20 mol % sterol in phosphatidylcholines. However, the orientation and molecular order parameter of cholesterol in DMPA are similar to those found in dimyristoylphosphatidylcholine.
Subject(s)
Cholesterol/chemistry , Glycerophospholipids , Membrane Lipids/chemistry , Membranes, Artificial , Phosphatidic Acids/chemistry , Biophysical Phenomena , Biophysics , Hydrogen/chemistry , Hydrogen-Ion Concentration , In Vitro Techniques , Lipid Bilayers/chemistry , Magnetic Resonance Spectroscopy , Phosphorus/chemistry , Protons , TemperatureABSTRACT
An expression for the C-C bond order parameter, SCC, of membrane hydrocarbon chains has been derived from the observed C-D bond order parameters. It allows calculation of the probability of each of the C-C bond rotamers and, consequently, the number of gauche defects per chain as well as their projected average length onto the bilayer normal, thus affording the calculation of accurate hydrophobic bilayer thicknesses. The effect of temperature has been studied on dilauroyl-, dimyristoyl-, and dipalmitoylphosphatidylcholine (DLPC, DMPC, DPPC) membranes, as has the effect of cholesterol on DMPC. The salient results are as follows: 1) an odd-even effect is observed for the SCC versus carbon position, k, whose amplitude increases with temperature; 2) calculation of SCC, from nonequivalent deuterons on the sn-2 chain of lipids, SCC2, leads to negative values, indicating the tendency for the C1-C2 bond to be oriented parallel to the bilayer surface; this bond becomes more parallel to the surface as the temperature increases or when cholesterol is added; 3) calculation on the sn-2 chain length can be performed from C1 to Cn, where n is the number of carbon atoms in the chain, and leads to 10.4, 12.2, and 13.8 A for DLPC, DMPC, and DPPC close to the transition temperature, TC, of each of the systems and to 9.4, 10.9, and 12.6 for T-TC = 30-40 degrees C, respectively; 4) separation of intra- and intermolecular motions allows quantitation of the number of gauche defects per chain, which is equal to 1.9, 2.7, and 3.5 for DLPC, DMPC, and DPPC near TC and to 2.7, 3.5, and 4.4 at T-TC = 30-40 degrees C, respectively. Finally, the validity of our model is discussed and compared with previously published models.
Subject(s)
Cholesterol , Lipid Bilayers/chemistry , Membranes , Models, Biological , Phosphatidylcholines/chemistry , 1,2-Dipalmitoylphosphatidylcholine/chemistry , Dimyristoylphosphatidylcholine/chemistry , Mathematics , Models, Molecular , Molecular Conformation , Reproducibility of Results , Structure-Activity RelationshipABSTRACT
Using a combination of X-ray diffraction data from oriented films and multilamellar liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) in the subgel phase, we have established the presence of a 2D molecular lattice containing two lipid molecules. The proposed 2D lattice is consistent with all the X-ray diffraction data on the subgel phase of DPPC available in the literature. In this phase, the DPPC molecules are ordered in the plane of the bilayer and are also found to be positionally correlated across a single bilayer but not with those in adjacent bilayers. We also present the possible molecular arrangements for the proposed lattice.
Subject(s)
1,2-Dipalmitoylphosphatidylcholine/chemistry , Lipid Bilayers , Magnetic Resonance Spectroscopy , X-Ray DiffractionABSTRACT
Light scattering and 31P-NMR have been used to monitor the effect of the bee-toxin, melittin, on phosphatidylcholine (PC) bilayers of variable acyl chain length (from C16:0 to C20:0). Melittin interacts with all lipids provided the interaction is initiated in the lipid fluid phase. For low-to-moderate amounts of toxin (lipid-peptide molar ratios, Ri > or = 15), the system takes the form of large spheroidal vesicles, in the fluid phase, whose radius increases from 750 A with dipalmitoyl-PC (DPPC) to 1500 A with diarachinoyl-PC (DAPC). These vesicles fragment into small discoids of 100-150 A radius when the system is cooled down below Tc (the gel-to-fluid phase transition temperature). Little chain length dependence is observed for the small objects. Small structures are also detected independently of the physical state of lipids (gel or fluid) when Ri < or = 5 and provided the interaction has been made above Tc. Small discs clearly characterized for DPPC and distearoyl-PC (DSPC) lipids are much less stable with DAPC. However in the long term, all these small structures fuse into large lipid lamellae. Discs are thermodynamically unstable and kinetics of disappearance of the small lipid-toxin complexes increases as the chain length increases in the sense: DAPC >> DSPC > DPPC. Kinetics of fusion of the small discs into extended bilayers is described by a pseudo-first-order law involving a lag time after which fusion starts. Increasing the chain length decreases the lag time and increases the rate of fusion. Formation of both the large vesicles in the fluid phase and the small discs in the gel phase as well as their stability is discussed in terms of relative shapes and dynamics of both lipids and toxin.
Subject(s)
Lipid Bilayers , Melitten/chemistry , Phosphatidylcholines/chemistry , 1,2-Dipalmitoylphosphatidylcholine/chemistry , Drug Stability , Kinetics , Light , Magnetic Resonance Spectroscopy , Molecular Conformation , Phosphorus , Scattering, Radiation , Structure-Activity Relationship , ThermodynamicsABSTRACT
Solid-state deuterium and phosphorus-31 nuclear magnetic resonance studies of deuterium-labeled beta--[2,2',3,4,4',6-2H6]-cholesterol and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine have been undertaken to monitor the action of melittin on model membranes containing 30 mol% cholesterol, both at the molecular and macroscopic level. Cholesterol totally inhibits the toxin-triggered formation of large unilamellar vesicles and strongly restricts the appearance of small discs. The latter remain stable over a wide temperature range (20-60 degrees C) because of an increase in their cholesterol content as the temperature increases. This process is related to a constant disc hydrophobic thickness of approximately 29 A. The system, when not in the form of discs, appears to be composed of very large vesicles on which melittin promotes magnetically induced ellipsoidal deformation. This deformation is the greatest when the maximum of discs is observed. A model to describe both the disc formation and stability is proposed.
Subject(s)
1,2-Dipalmitoylphosphatidylcholine/chemistry , Cholesterol/chemistry , Lipid Bilayers/chemistry , Melitten/pharmacology , Animals , Biophysical Phenomena , Biophysics , Deuterium , In Vitro Techniques , Magnetic Resonance Spectroscopy , Membranes, Artificial , Models, Chemical , Phosphorus , TemperatureABSTRACT
Neutron scattering experiments have been performed on oriented dipalmitoylphosphatidylcholine (DPPC) bilayers containing diphenylhexatriene (DPH) or its trimethylammonium analog (TMA-DPH). DPH and TMA-DPH were either protonated or deuterated in one of the phenyl rings which afforded by using proton-deuterium contrast methods the location of these fluorescent probes in the model membrane. Both probes exhibit bimodal distributions in DPPC. The position, population and orientation in the two sites vary depending upon the physical state of the bilayer (gel or fluid) and the presence or absence of the TMA group. In gel (L beta') phase lipids DPH is located close and parallel to the bilayer surface (site I) and near the bilayer center, oriented at approximately 30 degrees with respect to the normal to the surface (site II). On going to the fluid (L alpha) phase, a distribution of orientations around the parallel to the surface is only observed for site II. Orientation of DPH in site I is unchanged. In the gel phase TMA-DPH is found in a position close and parallel to the bilayer surface (site I) and in a position (site II) oriented at an angle of approximately 25 degrees with respect to the bilayer normal, with the trimethylammonium group anchored in the head group domain. On going to the fluid phase there is a change in molecular orientation of each of the sites. In site I the molecule penetrates deeper in the bilayer and adopts a approximately 20 degrees tilt with respect to the surface, with an orientational distribution of +/- 10 degrees. In site II the molecule becomes perpendicular to the membrane surface. Changes in population of sites, both with DPH and TMA-DPH, are observed on going from low to high temperatures. They are however difficult to quantitate due to experimental conditions. The H2O-2H2O exchange experiments afforded an estimate of the water layer thickness as well as the maximum penetration of water into the interior of the bilayer.