Health-related quality of life and clinical severity in people with epidermolysis bullosa - a proposal for assessing nutritional compromise by body mass index (birmingham Epidermolysis bullosa Severity Score) / Calidad de vida relacionada con la salud y gravedad clínica en personas con epidermólisis bullosa: una propuesta para evaluar el deterioro nutricional por el índice de masa corporal (Birmingham Epidermolysis Bullosa Severity Score)

Introduction and objectives: epidermolysis bullosa (EB) is a rare genetic disease characterised by skin fragility with blisters and erosions on the skin and/or mucous membranes. People with EB often experience several extracutaneous manifestations, including clinical and health-related quality of life (HRQoL) complications. Herein, we evaluate their HRQoL and clinical severity and propose an objective criterion for estimating nutritional compromise using the Birmingham Epidermolysis Bullosa Severity Score (BEBS) tool. Methods: this series of cases included people with EB, monitored by a multi-professional team. Clinical severity was assessed with the BEBS, using body mass index ranges by age, as an objective proposal, to estimate the degree of nutritional compromise. To assess HRQoL, the Children's Dermatology Life Quality Index (individuals aged 4-16 years) and the Quality of Life Evaluation in Epidermolysis Bullosa – Brazilian Portuguese (individuals 17 years and over) were used. Results: of the nine individuals with recessive dystrophic EB (88.9 % female and 12.91 (SD = 11.71) years), the mean total BEBS score was 24.47 (SD = 12.80) points on a scale of 0 to 100 points. Six participants had significant nutritional compromise according to the proposed criteria. Five of the six participants evaluated for HRQoL reported experiencing some impact, with individuals aged 17 and over being more affected and with greater clinical severity. Conclusions: individuals with greater clinical severity of EB experience a more significant impact on their HRQoL. The proposed quantitative criteria for assessing nutritional compromise may help standardise assessments by professionals monitoring the nutritional status of individuals with EB. Keywords: Quality of life. Epidermolysis bullosa. Body mass index.(AU)

Introducción y objetivos: la epidermólisis bullosa (EB) es una rara enfermedad genética caracterizada por fragilidad de la piel con ampollas yerosiones. Las personas con EB experimentan manifestaciones extracutáneas y complicaciones clínicas y de calidad de vida relacionada con la salud (CVRS). Evaluamos la CVRS y la gravedad clínica y proponemos un criterio objetivo para estimar el deterioro nutricional con la herramienta Birmingham Epidermolysis Bullosa Severity Score (BEBS). Métodos: esta serie de casos incluyó pacientes con EB monitoreadas por un equipo multiprofesional. Se evaluó la gravedad clínica con el BEBS utilizando rangos de índice de masa corporal por edad. Para evaluar la CVRS se utilizaron el Children's Dermatology Life Quality Index (individuos de 4 a 16 años) y el Quality of Life Evaluation in Epidermolysis Bullosa – Brazilian Portuguese (individuos de 17 años y más). Resultados: de los nueve individuos con EB distrófica recesiva (88,9 % mujeres y 12,91 (DE = 11,71) años), la puntuación total media del BEBS fue de 24,47 (DE = 12,80) puntos en una escala de 0 a 100 puntos. Seis participantes tenían un deterioro nutricional significativo según los criterios propuestos. Cinco de los seis participantes evaluados en la CVRS informaron experimentar algún impacto, siendo los individuos de 17 años y más los más afectados y con mayor gravedad clínica. Conclusiones: los pacientes con mayor gravedad clínica experimentan un impacto más significativo en su CVRS. Los criterios cuantitativos propuestos para evaluar el deterioro nutricional pueden ayudar a estandarizar las evaluaciones de los profesionales que monitorean el estadonutricional de las personas con EB.

Health-related quality of life and clinical severity in people with epidermolysis bullosa - A proposal for assessing nutritional compromise by body mass index (Birmingham Epidermolysis Bullosa Severity Score).

Introduction: Introduction and objectives: epidermolysis bullosa (EB) is a rare genetic disease characterised by skin fragility with blisters and erosions on the skin and/or mucous membranes. People with EB often experience several extracutaneous manifestations, including clinical and health-related quality of life (HRQoL) complications. Herein, we evaluate their HRQoL and clinical severity and propose an objective criterion for estimating nutritional compromise using the Birmingham Epidermolysis Bullosa Severity Score (BEBS) tool. Methods: this series of cases included people with EB, monitored by a multi-professional team. Clinical severity was assessed with the BEBS, using body mass index ranges by age, as an objective proposal, to estimate the degree of nutritional compromise. To assess HRQoL, the Children's Dermatology Life Quality Index (individuals aged 4-16 years) and the Quality of Life Evaluation in Epidermolysis Bullosa - Brazilian Portuguese (individuals 17 years and over) were used. Results: of the nine individuals with recessive dystrophic EB (88.9 % female and 12.91 (SD = 11.71) years), the mean total BEBS score was 24.47 (SD = 12.80) points on a scale of 0 to 100 points. Six participants had significant nutritional compromise according to the proposed criteria. Five of the six participants evaluated for HRQoL reported experiencing some impact, with individuals aged 17 and over being more affected and with greater clinical severity. Conclusions: individuals with greater clinical severity of EB experience a more significant impact on their HRQoL. The proposed quantitative criteria for assessing nutritional compromise may help standardise assessments by professionals monitoring the nutritional status of individuals with EB. Keywords: Quality of life. Epidermolysis bullosa. Body mass index.

Introducción: Introducción y objetivos: la epidermólisis bullosa (EB) es una rara enfermedad genética caracterizada por fragilidad de la piel con ampollas y erosiones. Las personas con EB experimentan manifestaciones extracutáneas y complicaciones clínicas y de calidad de vida relacionada con la salud (CVRS). Evaluamos la CVRS y la gravedad clínica y proponemos un criterio objetivo para estimar el deterioro nutricional con la herramienta Birmingham Epidermolysis Bullosa Severity Score (BEBS). Métodos: esta serie de casos incluyó pacientes con EB monitoreadas por un equipo multiprofesional. Se evaluó la gravedad clínica con el BEBS utilizando rangos de índice de masa corporal por edad. Para evaluar la CVRS se utilizaron el Children's Dermatology Life Quality Index (individuos de 4 a 16 años) y el Quality of Life Evaluation in Epidermolysis Bullosa ­ Brazilian Portuguese (individuos de 17 años y más). Resultados: de los nueve individuos con EB distrófica recesiva (88,9 % mujeres y 12,91 (DE = 11,71) años), la puntuación total media del BEBS fue de 24,47 (DE = 12,80) puntos en una escala de 0 a 100 puntos. Seis participantes tenían un deterioro nutricional significativo según los criterios propuestos. Cinco de los seis participantes evaluados en la CVRS informaron experimentar algún impacto, siendo los individuos de 17 años y más los más afectados y con mayor gravedad clínica. Conclusiones: los pacientes con mayor gravedad clínica experimentan un impacto más significativo en su CVRS. Los criterios cuantitativos propuestos para evaluar el deterioro nutricional pueden ayudar a estandarizar las evaluaciones de los profesionales que monitorean el estado nutricional de las personas con EB.

Long-Term Lifestyle Habits and Quality of Life after Roux-in-Y Gastric Bypass in Brazilian Public versus Private Healthcare Systems: Beyond Weight Loss.

Lifestyle and health-related quality of life (HRQoL) are good markers of surgical obesity treatment. This study aimed to investigate the lifestyle and HRQoL of patients at least five years after Roux-en-Y gastric bypass in public (SUS) and private (PVT) Brazilian healthcare systems. In this cross-sectional study, weight loss (WL), % of excess WL (%EWL), diet quality, physical activity, alcohol consumption, and HRQoL were evaluated. Analysis of covariance, binary and multinomial logistic regression, adjusted for confounders, were performed. The SUS group had more vulnerable socioeconomic statuses than the PVT group. Total %WL and % EWL were 24.64 ± 0.99% and 60.46 ± 2.41%, respectively, without difference between groups. In the Pain/Discomfort and Anxiety/Depression domains of HRQoL, more than 50% reported moderate problems without differences between groups. Processed food ingestion was higher in the PVT (132.10 ± 60.15 g/1000 kcal) than in the SUS (103.43 ± 41.72 g/1000 kcal), however, without statistical significance (p = 0.093). The PVT group showed lower physical activity (OR: 0.23; 95%CI: 0.87-0.63; p = 0.004) and a higher risk of alcohol-related problems (OR: 3.23; 95%CI; 1.03-10.10; p = 0.044) compared to SUS group. Participants generally achieved satisfactory WL, regardless of healthcare systems. However, PVT participants had unfavorable lifestyle characteristics, highlighting the need for studies investigating environmental issues post-bariatric surgery.

Factors associated with diet quality among Brazilian individuals with cardiovascular diseases.

BACKGROUND: An individual's dietary pattern contributes in different ways to the prevention and control of recurrent cardiovascular events. However, the quality of the diet is influenced by several factors. The present study aimed to evaluate the quality of the diet of individuals with cardiovascular diseases and determine whether there is an association between sociodemographic and lifestyle factors. METHODS: This is a cross-sectional study carried out with individuals with atherosclerosis (coronary artery disease, cerebrovascular disease or peripheral arterial disease) recruited from 35 reference centres for the treatment of cardiovascular disease in Brazil. Diet quality was assessed according to the Modified Alternative Healthy Eating Index (mAHEI) and stratified into tertiles. For comparing two groups, the Mann-Whitney or Pearson's chi-squared tests were used. However, for comparing three or more groups, analysis of variance or Kruskal-Wallis was used. For the confounding analysis, a multinomial regression model was used. p < 0.05 was considered statistically significant. RESULTS: In total, 2360 individuals were evaluated: 58.5% male and 64.2% elderly. The median (interquartile range [IQR]) of the mAHEI was 24.0 (20.0-30.0), ranging from 0.4 to 56.0 points. When comparing the odds ratios (ORs) for the low (first tertile) and medium (second tertile) diet quality groups with the high-quality group (third tertile), it was observed that there was an association between diet quality with a family income of 1.885 (95% confidence intervals [CI] = 1.302-2.729) and 1.566 (95% CI = 1.097-2.235), as well as physical activity of 1.391 (95% CI = 1.107-1.749) and 1.346 (95% CI = 1.086-1.667), respectively. In addition, associations were observed between diet quality and region of residence. CONCLUSIONS: A low-quality diet was associated with family income, sedentarism and geographical area. These data are extremely relevant to assist in coping with cardiovascular disease because they enable an assessment of the distribution of these factors in different regions of the country.

Factors associated with diet quality among Brazilian individuals with cardiovascular diseases

BACKGROUND: An individual's dietary pattern contributes in different ways to the prevention and control of recurrent cardiovascular events. However, the quality of the diet is influenced by several factors. The present study aimed to evaluate the quality of the diet of individuals with cardiovascular diseases and determine whether there is an association between sociodemographic and lifestyle factors. METHODS: This is a cross-sectional study carried out with individuals with atherosclerosis (coronary artery disease, cerebrovascular disease or peripheral arterial disease) recruited from 35 reference centres for the treatment of cardiovascular disease in Brazil. Diet quality was assessed according to the Modified Alternative Healthy Eating Index (mAHEI) and stratified into tertiles. For comparing two groups, the Mann-Whitney or Pearson's chi-squared tests were used. However, for comparing three or more groups, analysis of variance or Kruskal-Wallis was used. For the confounding analysis, a multinomial regression model was used. p < 0.05 was considered statistically significant. RESULTS: In total, 2360 individuals were evaluated: 58.5% male and 64.2% elderly. The median (interquartile range [IQR]) of the mAHEI was 24.0 (20.0-30.0), ranging from 0.4 to 56.0 points. When comparing the odds ratios (ORs) for the low (first tertile) and medium (second tertile) diet quality groups with the high-quality group (third tertile), it was observed that there was an association between diet quality with a family income of 1.885 (95% confidence intervals [CI] = 1.302-2.729) and 1.566 (95% CI = 1.097-2.235), as well as physical activity of 1.391 (95% CI = 1.107-1.749) and 1.346 (95% CI = 1.086-1.667), respectively. In addition, associations were observed between diet quality and region of residence. CONCLUSIONS: A low-quality diet was associated with family income, sedentarism and geographical area. These data are extremely relevant to assist in coping with cardiovascular disease because they enable an assessment of the distribution of these factors in different regions of the country.

Development and Validation of Protocol Based on Brazilian Dietary Guidelines for Adults with Diabetes Mellitus Who Attended Primary Health Care.

BACKGROUND: To date, there is no protocol providing dietary guidelines to assist health care professionals in counseling Brazilian individuals with Diabetes Mellitus (DM) assisted in primary health care (PHC) according to the Dietary Guidelines for the Brazilian Population (DGBP). Therefore, this study aimed to develop and validate a protocol based on the DGBP for health care, non-nutritionist professionals in counseling adults with DM in PHC. METHODS: We systematized the recommendations published in the DGBP, the Diabetes Brazilian Society guidelines, and the scientific literature regarding food and nutrition needs of adults with DM. The clarity and relevance were validated by an expert panel (n = 19) and the understanding and applicability were validated by PHC professionals (n = 12). The degree of agreement of the experts was assessed using a Content Validity Index (CVI). Items receiving CVI > 0.8 were considered appropriate. RESULTS: The protocol consisted of six dietary recommendations that encouraged the daily consumption of beans, vegetables, and fruits, advised the avoidance of sugar-sweetened beverages and ultra-processed foods, stimulated eating in appropriate environments, and gave additional guidance addressed to the particularities of DM. The protocol clarity, relevance, and applicability were successfully validated. CONCLUSION: The protocol supports health care, non-nutritionist professionals in the guidance of dietary recommendations and promoting adequate and healthy eating habits for adults with DM in PHC.

FGF Ligands and Receptors in Osteochondral Tissues of the Temporomandibular Joint in Young and Aging Mice.

OBJECTIVE: Fibroblast growth factors (FGFs) are a family of 22 proteins and 4 FGF receptors (FGFRs) that are crucial elements for normal development. The contribution of different FGFs and FGFRs for the homeostasis or disease of the cartilage from the mandibular condyle is unknown. Therefore, our goal was to characterize age-related alterations in the protein expression of FGF ligands and FGFRs in the mandibular condyle of mice. METHOD: Mandibular condyles of 1-, 6-, 12-, 18-, and 24-month-old C57BL/6J male mice (5 per group) were collected and histologically sectioned. Immunofluorescence for FGFs that have been reported to be relevant for chondrogenesis (FGF2, FGF8, FGF9, FGF18) as well as the activated/phosphorylated FGFRs (pFGFR1, pFGFR3) was carried out. RESULTS: FGF2 and FGF8 were strongly expressed in the cartilage and subchondral bone of 1-month-old mice, but the expression shifted mainly to the subchondral bone as mice aged. FGF18 and pFGFR3 expression was limited to the cartilage of 1-month-old mice only. Meanwhile, pFGFR1 and FGF9 were mostly limited to the cartilage with a significant increase in expression as mice aged. CONCLUSIONS: Our results indicate FGF2 and FGF8 are important growth factors for mandibular condylar cartilage growth in young mice but with limited role in the cartilage of older mice. In addition, the increased expression of pFGFR1 and FGF9 and the decreased expression of pFGFR3 and FGF18 as mice aged suggest the association of these factors with aging and osteoarthritis of the cartilage of the mandibular condyle.

Assessment of nutrient intakes of children and adolescents with recessive dystrophic epidermolysis bllosa, severe subtype / Evaluación de la ingesta de nutrientes de niños y adolescentes con epidermólisis bullosa distrófica recesiva, subtipo severo

Background and aims: epidermolysis bullosa (EB) is a rare disease presenting with skin fragility and formation of blisters. Constant presence of skin lesions, loss of blood, wastage of heat through the open skin, increased turnover of proteins and infections, result in increased energy, protein, and micronutrient needs. This study investigated the intake adequacy of energy, protein, zinc, iron, and dietary fiber in children with epidermolysis bullosa. Methods: this cross-sectional, analytical study was conducted with children and adolescents of up to 18 years of age with recessive dystrophic EB, severe subtype. Demographic data and clinical manifestations affecting food consumption were collected. Nutritional assessment was performed through anthropometric data. We used a formula to estimate specific energy needs for EB, and ratio of skin lesions was also evaluated to assist in estimating energy needs. A protein adequacy of 115 % to 200 % of the Recommended Dietary Allowance was considered for the adequacy of protein intake. And the Dietary Reference Intake tables according to sex and age were used as a reference for micronutrients. Intake assessment was performed using seven consecutive daily food records. Sip feed consumption was considered for intake assessment. Results: all patients showed undernutrition and presented at least three clinical symptoms that affect food consumption: pseudosyndactyly, microstomy, and blisters in the oral cavity. Sip feed constituted between 20 % and 50 % of the patients’ energy intake. Intake of iron and zinc was adequate for most patients (confidence of adequacy ≥ 0.85), while fiber intake was below the reference value. (AU)

Antecedentes y objetivos: la epidermólisis bullosa (EB) es una enfermedad rara que se manifiesta con fragilidad cutánea y formación de ampollas. La presencia constante de lesiones en la piel, la pérdida de sangre, la pérdida de calor a través de la piel abierta, el aumento de la pérdidas de proteínas y las infecciones hacen que aumenten las necesidades de energía, proteínas y micronutrientes. Este estudio investigó la adecuación de la ingesta de energía, proteínas, zinc, hierro y fibra dietética en niños con EB. Métodos: este estudio analítico transversal se llevó a cabo con niños y adolescentes de hasta 18 años de edad con el subtipo grave de la EB distrófica recesiva. Se recogieron los datos demográficos y las manifestaciones clínicas que afectan al consumo de alimentos. La evaluación nutricional se realizó mediante datos antropométricos. Se utilizó una fórmula para estimar las necesidades energéticas específicas de la EB y también se evaluó la proporción de las lesiones cutáneas para ayudar a estimar las necesidades energéticas. Para la adecuación de la ingestade proteínas se consideró entre el 115 y el 200 % de la ingesta dietética recomendada. Y como referencia para los micronutrientes se utilizaron las tablas de ingesta dietética de referencia según el sexo y la edad. La evaluación de la ingesta se realizó mediante siete registros diarios consecutivos de alimentos. Para la evaluación de la ingesta se tuvo en cuenta el consumo de suplementos nutricionales. Resultados: todos los pacientes mostraban desnutrición y presentaban al menos tres síntomas clínicos que afectaban al consumo de alimentos:pseudosindactilia, microstomía y ampollas en la cavidad oral. Los suplementos nutricionales constituían entre el 20 y el 50 % de la ingesta energética de los pacientes. La ingesta de hierro y zinc era adecuada para la mayoría de los pacientes (confianza de adecuación ≥ 0,85), mientras que la ingesta de fibra estuvo por debajo del valor de referencia. (AU)

Dynamic changes in transcriptome during orthodontic tooth movement.

OBJECTIVES: The objective of this study was to determine global changes in gene expression with next generation sequencing (NGS) in order to assess the biological effects of orthodontic tooth movement (OTM) on alveolar bone in a rat model. MATERIALS AND METHODS: Thirty-five Wistar rats (age 14 weeks) were used in the study. The OTM was performed using closed coil Nickel-Titanium spring to apply a mesial force on maxillary first molars of 8-10 g. Three hours, 1, 3, 7 and 14 days after the placement of the appliance, rats were killed at each time point respectively. The alveolar bone, around left maxillary first molar, were excised on compression side. The samples were immediately frozen in liquid nitrogen for subsequent RNA extraction. Total RNA samples were prepared for mRNA sequencing using the Illumina kit. RNA-Seq reads were aligned to the rat genomes using the STAR Aligner and bioinformatic analysis was performed. RESULTS: A total of 18 192 genes were determined. Day 1 has the highest number of differentially expressed genes (DEGs) observed with more upregulated than downregulated genes. A total of 2719 DEGs were identified to use as input for the algorithm. Six distinct clusters of temporal patterns were observed representing proteins that were differentially regulated indicating different expression kinetics. Principal component analysis (PCA) showed distinct clustering by time points and days 3, 7 and 14 share similar gene expression pattern. CONCLUSIONS: Distinct gene expression pattern was observed at different time points studied. Hypoxia, inflammation and bone remodelling pathways are major mechanisms behind OTM.

Assessment of nutrient intakes of children and adolescents with recessive dystrophic epidermolysis bllosa, severe subtype.

Introduction: Background and aims: epidermolysis bullosa (EB) is a rare disease presenting with skin fragility and formation of blisters. Constant presence of skin lesions, loss of blood, wastage of heat through the open skin, increased turnover of proteins and infections, result in increased energy, protein, and micronutrient needs. This study investigated the intake adequacy of energy, protein, zinc, iron, and dietary fiber in children with epidermolysis bullosa. Methods: this cross-sectional, analytical study was conducted with children and adolescents of up to 18 years of age with recessive dystrophic EB, severe subtype. Demographic data and clinical manifestations affecting food consumption were collected. Nutritional assessment was performed through anthropometric data. We used a formula to estimate specific energy needs for EB, and ratio of skin lesions was also evaluated to assist in estimating energy needs. A protein adequacy of 115 % to 200 % of the Recommended Dietary Allowance was considered for the adequacy of protein intake. And the Dietary Reference Intake tables according to sex and age were used as a reference for micronutrients. Intake assessment was performed using seven consecutive daily food records. Sip feed consumption was considered for intake assessment. Results: all patients showed undernutrition and presented at least three clinical symptoms that affect food consumption: pseudosyndactyly, microstomy, and blisters in the oral cavity. Sip feed constituted between 20 % and 50 % of the patients' energy intake. Intake of iron and zinc was adequate for most patients (confidence of adequacy ≥ 0.85), while fiber intake was below the reference value. Conclusions: this study underscores the importance of nutritional monitoring for EB patients, which is often interpreted as a skin disease but has enormous nutritional repercussions.

Introducción: ínas.Antecedentes y objetivos: la epidermólisis bullosa (EB) es una enfermedad rara que se manifiesta con fragilidad cutánea y formación de ampollas. La presencia constante de lesiones en la piel, la pérdida de sangre, la pérdida de calor a través de la piel abierta, el aumento de la pérdidas de proteínas y las infecciones hacen que aumenten las necesidades de energía, proteínas y micronutrientes. Este estudio investigó la adecuación de la ingesta de energía, proteínas, zinc, hierro y fibra dietética en niños con EB. Métodos: este estudio analítico transversal se llevó a cabo con niños y adolescentes de hasta 18 años de edad con el subtipo grave de la EB distrófica recesiva. Se recogieron los datos demográficos y las manifestaciones clínicas que afectan al consumo de alimentos. La evaluación nutricional se realizó mediante datos antropométricos. Se utilizó una fórmula para estimar las necesidades energéticas específicas de la EB y también se evaluó la proporción de las lesiones cutáneas para ayudar a estimar las necesidades energéticas. Para la adecuación de la ingesta de proteínas se consideró entre el 115 y el 200 % de la ingesta dietética recomendada. Y como referencia para los micronutrientes se utilizaron las tablas de ingesta dietética de referencia según el sexo y la edad. La evaluación de la ingesta se realizó mediante siete registros diarios consecutivos de alimentos. Para la evaluación de la ingesta se tuvo en cuenta el consumo de suplementos nutricionales. Resultados: todos los pacientes mostraban desnutrición y presentaban al menos tres síntomas clínicos que afectaban al consumo de alimentos: pseudosindactilia, microstomía y ampollas en la cavidad oral. Los suplementos nutricionales constituían entre el 20 y el 50 % de la ingesta energética de los pacientes. La ingesta de hierro y zinc era adecuada para la mayoría de los pacientes (confianza de adecuación ≥ 0,85), mientras que la ingesta de fibra estuvo por debajo del valor de referencia. Conclusiones: este estudio destaca la importancia del seguimiento nutricional de los pacientes con EB, que a menudo se interpreta como una enfermedad de la piel pero que tiene enormes repercusiones nutricionales.

Validation of a dietary advice protocol for adults with obesity in primary health care according to the Brazilian Dietary Guidelines.

BACKGROUND: Obesity is a non-communicable chronic disease which carries a high cost for the Brazilian Unified Health System. Standardized protocols can help primary health care (PHC) professionals manage the disease. OBJECTIVES: To describe the validation process of a protocol concerning dietary guidelines for adults with obesity in PHC by non-nutrition professionals. METHODS: A validation study of a dietary advice protocol consisting of 6 recommendations was conducted according to Brazilian Dietary Guidelines. The topics incorporated into the recommendations were submitted to a panel of judges for content validity and achieved a Scale Content Validity Index (S-CVI) score >0.80. Subsequently, an online workshop was held and consisted of guiding questions to adequately improve current protocols. Face validity was assessed in a mediation workshop conducted with PHC non-nutrition professionals. Following the validation process, necessary adjustments were made to the eating protocol. RESULTS: The validation process was conducted by a panel of 20 judges and 10 PHC professionals. The content was validated using a 0.98 S-CVI. The online workshop expert panel agreed the instrument provides a trustworthy foundation for appropriate dietary guidelines. Moreover, the judges suggested changes to the flowchart designed to support the professionals' decisions, discussed the absence of quantitative prescription guidelines, and offered additional suggestions to strengthen equity and encourage autonomy in non-nutrition healthcare professionals in PHC. CONCLUSION: This study describes the validation process of a dietary advice protocol for people with obesity, as well as the importance of its integration into PHC.

Obesity is a public health problem that generates high costs for the Brazilian Unified Health System. Dietary guidelines are essential for the care of people suffering from obesity in primary health care (PHC) and should be implemented by all health professionals. However, the absence of protocols based on the Brazilian Dietary Guidelines to support dietary advice constitutes a gap that can prevent the active participation of professionals without training in the field of nutrition. Thus, the objective of this study is to describe the validation process of a protocol for dietary advice for adults with obesity for non-nutrition professionals working in PHC. Researchers and practitioners participated in panels as judges and evaluated the initial text of the protocol, suggesting changes when necessary. Notes on the clarity of the text were also provided. After the requested adjustments, the protocol was validated and made available by the Brazilian Ministry of Health to PHC professionals.

Anabolic Response of Intermittent Parathyroid Hormone and Alendronate on the Osteochondral Tissue of TMJ.

OBJECTIVE: To characterize the effects of parathyroid hormone (PTH) and alendronate (Alend) on the osteochondral tissue of temporomandibular joint (TMJ). MATERIALS AND METHODS: Ninety-six male and female transgenic reporter mice, 4 to 5 weeks old were divided into 6 groups: (1) Control group: Saline was injected daily for 14 days; (2) PTH: PTH was injected daily for 14 days; (3) Alend: Alend was injected every alternate days for 14 days; (4) Combined PTH and Alend: PTH was injected daily and Alend injected every alternate days for 14 days; (5) PTH then Alend: PTH was injected daily for 14 days followed by Alend injections in alternate days for 14 days; and (6) PTH wait Alend: PTH was injected daily for 14 days. There was a waiting period of 1 week before administration of Alend in alternate days for 14 days. Mice were injected with 5-ethnyl-2'-deoxyuridine (EdU), 48 and 24 hours prior to euthanization. RESULTS: There was significant increase in bone volume and decrease in osteoclastic activity in groups in which Alend was administered after PTH in both gender. There was significant increase in cartilage thickness with PTH or Alend alone in females, whereas in males, PTH alone led to increase in cartilage thickness. Chondrocyte apoptosis was significantly decreased with PTH or Alend alone in both male and female. Matrix metallopeptidase 13, and aggreganase-2 (ADAMTS5) expression were significantly decreased with PTH and Alend alone in both gender. CONCLUSION: PTH and Alend administration causes anabolic effects in the osteochondral tissue of TMJ.

New Insights on the Association Between Socioeconomic Status and Weight Loss After Bariatric Surgery: a Systematic Review and Meta-analysis.

The effect of socioeconomic status (SES) on weight loss (WL) after bariatric surgery (BS) remains unclear. This systematic review and meta-analysis aimed to investigate the association between SES and WL at least 12 months after BS. This study included 53 observational studies (retrieved from databases in October 2021 and updated in February 2022) involving adults who underwent any type of BS; SES data and data regarding outcomes of weight loss were also retrieved. Our results revealed that white individuals had a higher percentage of excess WL than blacks (95% confidence interval [CI]: 3.25-10.99, heterogeneity index [I2] = 44.87% and 95% CI: 8.08-13.59, I2 = 0%, respectively; both p < 0.01) after 12 and 24 months of BS. In conclusion, only race/ethnicity was associated with WL after BS.

Sarcopenic obesity diagnosis by different criteria mid-to long-term post-bariatric surgery.

BACKGROUND/AIMS: The aim of this study was to apply the European Society for Clinical Nutrition and Metabolism/European Association for the Study of Obesity (ESPEN/EASO) consensus to identify sarcopenic obesity (SO) in adults mid to long-term post-Roux-en-Y gastric bypass (RYGB) using both dual-energy x-ray absorptiometry (DXA) and bioelectrical impedance analysis (BIA). Further, this approach was compared to accepted sarcopenia diagnostic criteria (Revised European Working Group on Sarcopenia in Older People [EWGSOP2] and Sarcopenia Definition and Outcomes Consortium [SDOC]). METHODS: This cross-sectional study included adults ≥2 years post-RYGB surgery. Obesity was diagnosed by excess fat mass (FM) for all diagnostic criteria. Agreement was evaluated using Cohen's Kappa. RESULTS: We evaluated 186 participants (90.9% female, median age 43.9 years, 6.8 years post-surgery), of which 60.2% (BIA), and 83.3% (DXA) had excess FM. Low muscle strength was not identified using absolute handgrip strength. The prevalence of SO by BIA or DXA, respectively, was 7.9% (95%CI 3.9-12.5), and 23.0% (95%CI 17.1-30.3) [ESPEN/EASO SO consensus]; 0.7% (95%CI 0-2.0), and 3.3% (95%CI 0.7-5.9) [EWGSOP2]; and 27.0% (95%CI 19.7-34.2), and 30.3% (95%CI 23.0-37.5) [SDOC]. Agreement between the ESPEN/EASO SO consensus and other diagnostic criteria was none to slight using DXA: EWGSOP2 k = 0.19; 95% CI 0.04-0.34, or SDOC k = 0.16; 95% CI -0.01-0.32. Moderate agreement was observed within the ESPEN/EASO SO consensus for BIA and DXA (k = 0.43; 95% CI 0.26-0.60). CONCLUSIONS: This is the first study to explore the prevalence of SO using the ESPEN/EASO criteria. We identified a high but variable prevalence of SO in post-bariatric surgery patients (7.9-23.0%), depending on the body composition technique used; prevalence was higher using DXA. Little agreement was observed for the diagnosis of SO using the three diagnostic criteria. Future studies are needed to explore the relationship between SO identified by the ESPEN/EASO consensus and health status/outcomes.

Effect of physical exercise on muscle strength in adults following bariatric surgery: A systematic review and meta-analysis of different muscle strength assessment tests.

Individuals following bariatric surgery are considered at high risk for the development of sarcopenic obesity (excess fat mass, low muscle mass and low physical function), and exercise may play an important role in its prevention and treatment. We systematically reviewed 5 scientific databases (Embase, Medline, Scopus, SPORTDiscus, and Web of Science) and 2 grey literature databases (ProQuest and Google Scholar) for clinical trials that evaluated the effect of exercise on muscle strength in adults following bariatric surgery and conducted a separate meta-analysis for studies that used different muscle strength tests. Random-effect models, restricted maximum likelihood method and Hedges' g were used. The review protocol was registered at the International Prospective Register of Systematic Reviews (PROSPERO) database (CRD42020152142). Fifteen studies were included (638 patients), none had a low risk of bias, and all were included in at least 1 of the 5 meta-analyses (repetition maximum [lower and upper limbs], sit-to-stand, dynamometer, and handgrip tests). Exercise interventions improved both upper (effect size, 0.71; 95% CI, 0.41-1.01; I2 = 0%) and lower (effect size, 1.37; 95% CI, 0.84-1.91; I2 = 46.14) limb muscle strength, as measured by repetition maximum tests. Results were similar for the sit-to-stand (effect size, 0.60; 95% CI, 0.20-1.01; I2 = 68.89%) and dynamometer (effect size, 0.46; 95% CI, 0.06-0.87; I2 = 31.03%), but not for the handgrip test (effect size, 0.11; 95% CI, -0.42-0.63; I2 = 73.27%). However, the certainty level of the meta-analyses was very low. Exercise with a resistance training component performed post bariatric surgery may improve muscle strength, which is related to sarcopenic obesity, functional capacity, and mortality risk, therefore should be included in the follow-up.

Hipertensão Arterial e Parâmetros Lipídicos, Glicídicos e de Adiposidade Associados em Adolescentes Escolares do Distrito Federal / Hypertension and Associated Lipid, Glucose, and Adiposity Parameters in School-Aged Adolescents in the Federal District, Brazil

Resumo Fundamento A prevalência de hipertensão arterial sistêmica (HAS) e de outros distúrbios metabólicos tem aumentado em indivíduos jovens. Entretanto, não há estudos representativos sobre esse assunto com a população do Distrito Federal (DF). Objetivo Estimar a prevalência de HAS e a sua associação com parâmetros lipídicos, glicídicos e de adiposidade em adolescentes do DF. Métodos Trata-se de um estudo observacional transversal com participantes do Estudo de Riscos Cardiovasculares em Adolescentes (ERICA). Foram avaliados pressão arterial, glicemia sanguínea, hemoglobina glicada, insulina, modelo de avaliação da homeostase da resistência à insulina (HOMA-IR), triglicerídeos, colesterol total, lipoproteína de alta densidade, lipoproteína de baixa densidade, índice de massa corporal (IMC) e perímetro da cintura, além de variáveis econômicas, demográficas e de maturação sexual. A análise de dados foi feita no software Stata e foi dividida nas seguintes etapas: análises descritiva, bruta e ajustada. Considerou-se p < 0,05. Resultados Foram incluídos 1.200 adolescentes com média de idade de 14,8 anos. A prevalência de HAS foi de 8% (intervalo de confiança de 95%: 6,3; 9,9). A maioria dos parâmetros se associou com a PA na análise bruta; na ajustada, os parâmetros glicídicos, lipídicos e de adiposidade mantiveram a associação, tendo IMC e HOMA-IR a maior magnitude na relação. Conclusão O estudo revelou elevada prevalência de HAS em adolescentes do DF, e os níveis pressóricos apresentaram-se associados a outros marcadores de perfil lipídico, glicídico e de adiposidade, evidenciando a relevância da vigilância em saúde para o planejamento de ações efetivas para a reversão do quadro e prevenção de novos casos.

Abstract Background The prevalence of hypertension and other metabolic disorders has increased in young individuals. However, no representative studies have been conducted in the population of the Federal District, Brazil. Objective To estimate the prevalence of hypertension and its association with lipid, glucose, and adiposity markers in school-aged adolescents living in the Federal District. Methods This cross-sectional study included participants of the Study of Cardiovascular Risks in Adolescents (Portuguese acronym, ERICA). Blood pressure, blood glucose, glycated hemoglobin, insulin, homeostatic model assessment for insulin resistance (HOMA-IR), triglycerides, total cholesterol, high-density lipoprotein, low-density lipoprotein, body mass index (BMI), waist circumference, and economic, demographic, and sexual maturity variables were assessed. The data were analyzed in Stata, and the analysis was divided into different stages: descriptive, crude, and adjusted. Significant results were set at p < 0.05. Results In total, 1,200 adolescents were included, and their mean age was 14.8 years. The prevalence of hypertension was 8% (95% confidence interval: 6.3; 9.9). Most parameters were associated with blood pressure in crude analysis. In adjusted analysis, glucose, lipid, and adiposity markers maintained the associations, and the highest magnitudes were those of BMI and HOMA-IR. Conclusion The study revealed a high prevalence of hypertension in adolescents living in the Federal District, and blood pressure levels were associated with other markers of lipid, glucose, and adiposity profile. The findings indicate the relevance of health surveillance for planning effective actions aimed at reversing this situation and preventing new cases.

Hypertension and Associated Lipid, Glucose, and Adiposity Parameters in School-Aged Adolescents in the Federal District, Brazil. / Hipertensão Arterial e Parâmetros Lipídicos, Glicídicos e de Adiposidade Associados em Adolescentes Escolares do Distrito Federal.

BACKGROUND: The prevalence of hypertension and other metabolic disorders has increased in young individuals. However, no representative studies have been conducted in the population of the Federal District, Brazil. OBJECTIVE: To estimate the prevalence of hypertension and its association with lipid, glucose, and adiposity markers in school-aged adolescents living in the Federal District. METHODS: This cross-sectional study included participants of the Study of Cardiovascular Risks in Adolescents (Portuguese acronym, ERICA). Blood pressure, blood glucose, glycated hemoglobin, insulin, homeostatic model assessment for insulin resistance (HOMA-IR), triglycerides, total cholesterol, high-density lipoprotein, low-density lipoprotein, body mass index (BMI), waist circumference, and economic, demographic, and sexual maturity variables were assessed. The data were analyzed in Stata, and the analysis was divided into different stages: descriptive, crude, and adjusted. Significant results were set at p < 0.05. RESULTS: In total, 1,200 adolescents were included, and their mean age was 14.8 years. The prevalence of hypertension was 8% (95% confidence interval: 6.3; 9.9). Most parameters were associated with blood pressure in crude analysis. In adjusted analysis, glucose, lipid, and adiposity markers maintained the associations, and the highest magnitudes were those of BMI and HOMA-IR. CONCLUSION: The study revealed a high prevalence of hypertension in adolescents living in the Federal District, and blood pressure levels were associated with other markers of lipid, glucose, and adiposity profile. The findings indicate the relevance of health surveillance for planning effective actions aimed at reversing this situation and preventing new cases.

FUNDAMENTO: A prevalência de hipertensão arterial sistêmica (HAS) e de outros distúrbios metabólicos tem aumentado em indivíduos jovens. Entretanto, não há estudos representativos sobre esse assunto com a população do Distrito Federal (DF). OBJETIVO: Estimar a prevalência de HAS e a sua associação com parâmetros lipídicos, glicídicos e de adiposidade em adolescentes do DF. MÉTODOS: Trata-se de um estudo observacional transversal com participantes do Estudo de Riscos Cardiovasculares em Adolescentes (ERICA). Foram avaliados pressão arterial, glicemia sanguínea, hemoglobina glicada, insulina, modelo de avaliação da homeostase da resistência à insulina (HOMA-IR), triglicerídeos, colesterol total, lipoproteína de alta densidade, lipoproteína de baixa densidade, índice de massa corporal (IMC) e perímetro da cintura, além de variáveis econômicas, demográficas e de maturação sexual. A análise de dados foi feita no software Stata e foi dividida nas seguintes etapas: análises descritiva, bruta e ajustada. Considerou-se p < 0,05. RESULTADOS: Foram incluídos 1.200 adolescentes com média de idade de 14,8 anos. A prevalência de HAS foi de 8% (intervalo de confiança de 95%: 6,3; 9,9). A maioria dos parâmetros se associou com a PA na análise bruta; na ajustada, os parâmetros glicídicos, lipídicos e de adiposidade mantiveram a associação, tendo IMC e HOMA-IR a maior magnitude na relação. CONCLUSÃO: O estudo revelou elevada prevalência de HAS em adolescentes do DF, e os níveis pressóricos apresentaram-se associados a outros marcadores de perfil lipídico, glicídico e de adiposidade, evidenciando a relevância da vigilância em saúde para o planejamento de ações efetivas para a reversão do quadro e prevenção de novos casos.

Vitamin E enriched diet increases the rate of orthodontic tooth movement.

INTRODUCTION: Vitamin E is a popular antioxidant suggested to affect bone turnover. However, the effects of a vitamin E enriched diet on the rate of tooth movement are unknown. Therefore, this study aimed to evaluate tooth movement in rats receiving a vitamin E enriched diet. In addition, we examined bone remodeling in experimental and control rats. METHODS: Thirty-two 6-week-old male rats were divided into 4 groups: (1) group 1 (n = 8): orthodontic tooth movement (OTM) for 4 days + regular diet; (2) group 2 (n = 8): OTM for 14 days + regular diet; (3) group 3 (n = 8): OTM for 4 days + vitamin E diet; and (4) group 4 (n = 8) - OTM for 14 days + vitamin E diet. Maxillary alveolar bones and femurs of rats were analyzed by microcomputed tomography and histology. RESULTS: Rats fed a vitamin E diet presented an increased OTM rate at days 4 and 14. We found an increased number of osteoclasts and decreased bone volume in the vitamin E diet group at day 14 of OTM. In addition, there was increased expression of the microphthalmia-associated transcription factor in the alveolar bone of the vitamin E diet group. In contrast, there was no difference in bone remodeling in femurs or alveolar bone at the control side. CONCLUSIONS: We found that an enriched vitamin E diet increases the rate of OTM in rats, suggesting that vitamin E may be useful as an avenue to accelerate OTM.

The food environment in schools and their immediate vicinities associated with excess weight in adolescence: A systematic review and meta-analysis.

AIM: The present study systematically reviewed the characteristics of the food environment in schools and their immediate vicinities associated with excess weight in adolescents. METHODS: We searched for relevant articles in seven databases. No restrictions were applied on language, publication date, and status of publication. The study selection process and data extraction were conducted by two authors independently. For meta-analyses, the random-effects model and the maximum-likelihood method were applied. RESULTS: A total of 9327 publications were identified from the initial search. Of these, 20 studies met the inclusion criteria and were included in the systematic review. The sale of food in school or immediate vicinity was associated with high BMI [Odds ratio (OR)= 1.14, 95% confidence interval (CI) 1.01, 2.06]. The availability of healthy food provided by school significantly decreased the odds of obesity [OR= 0.89, 95%CI 0.82, 0.96]. However, the presence of nutrition policies or programs at school was not associated with obesity (OR= 0.81, 95%CI 0.57, 1.16). CONCLUSIONS: This study highlights the need for improvement in school food environment, including restricting students' exposure to unhealthy foods. The quality of food (healthy vs unhealthy) provided or made available to adolescents in the school food environment influences their weight status. Therefore, promotion of healthy food programs and availability of healthy food at school would limit the prevalence of excess weight in adolescents.

Grazing Behavior Hinders Weight Loss in Long-Term Post Bariatric Surgery: a Cross-Sectional Study.

BACKGROUND: Grazing behavior is common in patients undergoing bariatric surgery (BS); however, little is known about grazing behavior in operated patients in the long term. We aimed to estimate grazing frequency and its association with weight loss and surgery response in patients who had undergone BS. METHODS: This cross-sectional study included 109 patients who had undergone Roux-en-Y gastric bypass at least 5 years previously. They answered questions about socioeconomic factors, surgical information, and adherence to treatment/follow-up. The Rep(eat) questionnaire was used for grazing assessment. Student's t-test and Pearson's chi-square test were used to evaluate differences between groups. Analysis of covariance and partial correlation were used to investigate grazing behavior, and weight loss outcomes were controlled for the elapsed time since BS, healthcare service type, current psychologist care status, and the presence of a constriction ring. RESULTS: The mean elapsed time since BS was 9.97 ± 2.39 years. Patients with grazing behavior (67%) comprised fewer surgery responders (p = 0.019), had lower total (%TWL, p = 0.005) and excess weight loss (%EWL, p = 0.029), and received less psychological counseling (45.5% vs. 54.5%, p = 0.042). Correlations were found between the presence of grazing and its subtypes and %EWL and %TWL (T = - 0.382, T = - 0.361, p < 0.001; compulsive grazing: T = - 0.358, T = - 0.342, p < 0.001, p = 0.001; non-compulsive grazing: T = - 0.333, T = - 0.311, p = 0.001, p = 0.003). No significant difference between patients with and without grazing behavior was found for previous/current diagnoses of psychological diseases or dietitian counseling. CONCLUSION: Grazing behavior is common in the long-term among patients who have undergone BS and is negatively related to weight loss parameters.