ABSTRACT
IntroducciónSe estudiaron los genotipos circulantes de Chlamydia trachomatis que causan infección en nuestra área geográfica con el fin de detectar posibles peculiaridades epidemiológicas.MétodosSe genotiparon 177 cepas obtenidas entre 2006 y 2008 empleando una reacción en cadena de la polimerasa con cebadores del gen ompA y posterior secuenciación.ResultadosLos genotipos más frecuentes fueron el E (45,3%), el D (15,3%), el G (10,2%) y el F (9,6%). Otros genotipos encontrados fueron B, H, I, J, K y LGV II.ConclusiónLa técnica empleada tuvo una alta rentabilidad (89%) (AU)
IntroductionCirculating Chlamydia trachomatis genotypes that cause infection in our geographic area were studied with the aim of detecting possible epidemiological peculiarities.MethodsA total of 177 strains obtained between 2006 and 2008 were genotyped using a PCR with primers targeting the ompA gene, and later sequenced.ResultsThe most frequent genotypes were: E (45.3%), D (15.3%), G (10.2%) and F (9.6%). Other genotypes found were: B, H, I, J, K and LGV II.ConclusionThe molecular assay used had a high yield (89%) (AU)
Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Chlamydia trachomatis/genetics , Genotype , SpainSubject(s)
Chlamydia Infections/microbiology , Chlamydia trachomatis/isolation & purification , DNA, Bacterial/isolation & purification , Immunomagnetic Separation/methods , Polymerase Chain Reaction/instrumentation , Adolescent , Adult , Aged , Automation , Chlamydia trachomatis/genetics , Computer Systems , Female , Humans , Immunomagnetic Separation/instrumentation , Male , Middle Aged , Polymerase Chain Reaction/methods , Proctitis/microbiology , Specimen Handling/instrumentation , Specimen Handling/methods , Urethritis/microbiology , Uterine Cervicitis/microbiology , Young AdultABSTRACT
INTRODUCTION: Circulating Chlamydia trachomatis genotypes that cause infection in our geographic area were studied with the aim of detecting possible epidemiological peculiarities. METHODS: A total of 177 strains obtained between 2006 and 2008 were genotyped using a PCR with primers targeting the ompA gene, and later sequenced. RESULTS: The most frequent genotypes were: E (45.3%), D (15.3%), G (10.2%) and F (9.6%). Other genotypes found were: B, H, I, J, K and LGV II. CONCLUSION: The molecular assay used had a high yield (89%).
Subject(s)
Chlamydia trachomatis/classification , Chlamydia trachomatis/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Chlamydia trachomatis/isolation & purification , Female , Genotype , Humans , Infant , Male , Middle Aged , Spain , Young AdultABSTRACT
We present two cases of P. falciparum malaria in visitors to tourist resorts on the East Coast of the Dominican Republic, traditionally believed to be an area without risk of malaria. In both patients the malaria was severe (with 20% parasitization in one) and there was a long interval between the onset of symptoms and diagnosis. These cases are possibly related (along with a further 17 reports by the Centers for Disease Control and Prevention) to an increase in the population of Anopheles sp as a consequence of increased rainfall and floods provoked by a hurricane in September 2004, as well as to the presence of a semi-immune population (Haitian immigrants working in the construction and tourist sectors). Both physicians and patients should be aware of this outbreak so that adequate precautions can be taken and early diagnoses can be made.
Subject(s)
Malaria, Falciparum/diagnosis , Plasmodium falciparum/isolation & purification , Adult , Animals , Antimalarials/therapeutic use , Dominican Republic , Female , Humans , Malaria, Falciparum/drug therapy , Male , Middle Aged , TravelABSTRACT
Se presentan 2 casos de paludismo por Plasmodium falciparum en viajeros a centros turísticos de la costa este de República Dominicana, considerados clásicamente como libres de riesgo de paludismo. En ambos casos, se trató de pacientes con paludismo grave (con una parasitación del 20% en uno de ellos), con un tiempo largo entre el inicio de la sintomatología y el diagnóstico. Es posible que estos casos (junto con otros 17 comunicados por los Center for Disease Control and Prevention [CDC]) estén relacionados con un aumento en la población de Anopheles sp. como consecuencia del aumento de lluvias e inundaciones provocados por el paso de un huracán en septiembre de 2004, junto con la existencia de población semiinmune (inmigrantes haitianos trabajando en la construcción y el sector turístico). Es muy importante que tanto los médicos como los pacientes sean conscientes de este brote para facilitar la toma adecuada de precauciones y un diagnóstico precoz (AU)
We present two cases of P. falciparum malaria in visitors to tourist resorts on the East Coast of the Dominican Republic, traditionally believed to be an area without risk of malaria. In both patients the malaria was severe (with 20% parasitization in one) and there was a long interval between the onset of symptoms and diagnosis. These cases are possibly related (along with a further 17 reports by the Centers for Disease Control and Prevention) to an increase in the population of Anopheles sp as a consequence of increased rainfall and floods provoked by a hurricane in September 2004, as well as to the presence of a semi-immune population (Haitian immigrants working in the construction and tourists sectors). Both physicians and patients should be aware of this outbreak so that adequate precautions can be taken and early diagnoses can be made (AU)
Subject(s)
Male , Female , Adult , Middle Aged , Humans , Plasmodium falciparum/pathogenicity , Malaria, Falciparum/diagnosis , Malaria, Falciparum/epidemiology , Dominican Republic/epidemiologyABSTRACT
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