ABSTRACT
The effects of p-tyrosol and Rhodiola rosea extract on the hemopoietic system were compared on a model of subacute lead intoxication. No significant differences between the activity of two preparations were revealed by the study of plasma membrane blebbing, apoptosis, and necrosis processes in bone marrow. At the same time, p-tyrosol exhibited a more pronounced effect upon lipid peroxidation and offered significant protection against lead intoxication.
Subject(s)
Bone Marrow Cells/drug effects , Phenylethyl Alcohol/analogs & derivatives , Phenylethyl Alcohol/pharmacology , Rhodiola/chemistry , Animals , Apoptosis/drug effects , Bone Marrow Cells/metabolism , Bone Marrow Cells/pathology , Lipid Peroxidation/drug effects , Male , Mice , Necrosis , Organometallic Compounds/toxicity , Plant Extracts/chemistry , Plant Extracts/pharmacologyABSTRACT
An in vivo study investigated the toxic effect of zinc chloride on murine spermatozoids. Acute and subacute intoxication was found to increase the quantity of TBC active products and to induce the growth of degenerative forms of spermatozoids.
Subject(s)
Chlorides/toxicity , Germ Cells/drug effects , Infertility, Male/chemically induced , Oxidative Stress/drug effects , Zinc Compounds/toxicity , Animals , Male , MiceABSTRACT
ATP activity in mouse bone marrow cells was studied in in vitro experiments. ATP in physiological and supraphysiological concentrations stimulated blebbing of the plasma membrane. Subacute administration of doxorubicin (inductor of oxidative stress) decreased cell sensitivity to ATP, which corresponded to changes in membrane lipid peroxidation.
Subject(s)
Adenosine Triphosphate/metabolism , Bone Marrow Cells/drug effects , Bone Marrow Cells/metabolism , Doxorubicin/pharmacology , Animals , Antineoplastic Agents/pharmacology , Lipid Peroxidation , Male , Mice , Microscopy, Phase-Contrast , Necrosis , Oxidative Stress , Time FactorsSubject(s)
Mutagenesis/drug effects , Oxidative Stress/drug effects , Zinc/toxicity , Animals , Apoptosis/drug effects , MiceABSTRACT
We studied in vitro cytostatic effect of doxorubicin on bone marrow cells preincubated with NAD and reduced glutathione. The presence of NAD and glutathione in the medium potentiated membrane toxicity of this antibiotic, but prevented its toxic effects on intracellular pool of pyridine nucleotides during oxidative stress.
Subject(s)
Bone Marrow Cells/drug effects , Doxorubicin/toxicity , Glutathione/pharmacology , NAD/pharmacology , Oxidative Stress , Animals , Antibiotics, Antineoplastic/toxicity , Bone Marrow Cells/physiology , Cell Membrane/metabolism , Lipid Peroxidation , Male , Mice , NecrosisABSTRACT
The cytotoxic effects of Nd-YAG laser with a wavelength of 533 nm were studied in mouse bone marrow cells in vitro. Laser exposure caused blebbing of bone marrow cell plasma membrane. Preliminary induction of cytochrome P-450 with 3-methylcholanthrene modified the cytotoxic effect of Nd-YAG laser.
Subject(s)
Bone Marrow Cells/radiation effects , Cytochrome P-450 Enzyme System/biosynthesis , Lasers/adverse effects , Methylcholanthrene/pharmacology , Radiation-Protective Agents/pharmacology , Animals , Bone Marrow Cells/enzymology , Bone Marrow Cells/ultrastructure , Cell Membrane/radiation effects , Cell Membrane/ultrastructure , Cells, Cultured , Drug Administration Schedule , Enzyme Induction , Injections, Intraperitoneal , Male , Malondialdehyde/analysis , Methylcholanthrene/administration & dosage , Mice , Mice, Inbred Strains , Radiation-Protective Agents/administration & dosage , Thiobarbituric Acid Reactive Substances/metabolismABSTRACT
We studied the effects of cis-dichlorodiammine platinum (II) on different pathways of cell death in cultured Ehrlich ascites carcinoma in vitro and on subsequent growth of transplanted tumor in vivo. One-hour incubation with the cytostatic modulated apoptosis in cell culture. However, exposure of cell culture to a minimal effective concentration of cis-platinum(II)diammine dichloride promoted the growth of transplanted tumor.
Subject(s)
Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Carcinoma, Ehrlich Tumor/drug therapy , Carcinoma, Ehrlich Tumor/pathology , Cisplatin/pharmacology , Animals , Antineoplastic Agents/therapeutic use , Cell Division/drug effects , Cisplatin/therapeutic use , Female , Mice , Tumor Cells, CulturedABSTRACT
The metabolic state of rat liver was studied in vivo 1, 6, and 18 h after single hyperthermia (42 degrees C). It was shown that intracellular compensatory reactions involved in restoration of the energy state and realization of endogenous cytoprotective mechanisms play a role during recovery.
Subject(s)
Fever/metabolism , Liver/metabolism , Animals , Female , Male , Perfusion , Rats , Time FactorsABSTRACT
The work shows the results of experimental study of the characteristics of serotonin regulation of cell proliferation and programmed cell death in bone-marrow cells cultivated in diffusion chambers. It was found that the change in cell colony and cluster formation and death induced by this biogenic enzyme is disturbed by acrylamide, an inducer of oxidizing stress. Nicotinamide levels out the effect of acrylamide, which is evidence of the existence of NAD-dependent mechanisms in the serotonin-mediated regulation of hemopoiesis.
Subject(s)
Bone Marrow Cells/drug effects , Oxidative Stress/drug effects , Receptors, Serotonin/drug effects , Xenobiotics/pharmacology , Acrylamide/pharmacology , Animals , Apoptosis/drug effects , Bone Marrow Cells/cytology , Cell Division/drug effects , Cells, Cultured , Diffusion Chambers, Culture , Dose-Response Relationship, Drug , Female , Male , Mice , Niacinamide/pharmacology , Serotonin/pharmacologySubject(s)
Acrylamide/toxicity , Hot Temperature/adverse effects , Shock/metabolism , Animals , Cytochrome P-450 Enzyme System/metabolism , Enzyme Induction , Male , Methylcholanthrene/pharmacology , Phenobarbital/pharmacology , Rats , Shock/enzymology , Shock/etiology , Shock/physiopathology , SleepABSTRACT
Study of mutagenic activity of butylacrylate and methyl methacrylate on bone marrow cells of rat was carried out. Mutagenic activity of both of them was noted, but the butylacrylate was more active.