[The investigation of the influence of cryopreservation and inbreeding on the variability of morphological characteristics of the evening-primrose biennial (Oenothera biennis L.)].

The effect of deep freezing of seeds at -196 degrees C (-320.8 degrees Fahrenheit) and inbreeding on the morphological characteristics of the evening-primrose biennal (Oenothera biennis L.), such as the size of plant parts and the amount of fruits, cauline nodes, and generative and vegetative shoots was investigated. The variation coefficients for these characteristics after treatment with low temperatures and inbreeding were calculated. It was shown that the characteristics of plant size show a low and a middle level of variability in the control group. The variation curves for these characteristics are similar to normal distribution curves. After stresses they slightly change or remain invariant. Large adventive shoots show a high level of variability. The distribution of the results in this case significantly differs from the normal. The branching of plants changes after both stress factors: the amount of all kinds of shoots decreases by half or even more.

[The effect of protease tissue inhibitor on the activity of pancreatic proteolytic enzymes in in-vitro experiments]. / Issledovanie vliianiia tkanevogo ingibitora proteaz na aktivnost' pankreaticheskikh proteoliticheskikh fermentov v opytakh in vitro.

The authors provide the data on in vitro experimental studies into the pharmacological properties of protease inhibitor obtained from bovine pancreas. It is shown that the preparation exerts an inhibitory action on pancreatic proteolytic enzymes such as trypsin, kallikrein, and elastase, drastically reduces the total proteolytic activity in pancreatic tissue homogenates. Comparison with the protease inhibitor gordox has demonstrated the inhibitory effect of both preparations to be practically similar.

[Comparative radioligand analysis of gene-engineered and human hypophyseal somatotropic hormones]. / Sravnitel'nyi radioligandnyi analiz genno-inzhenernogo i gipofizarnogo somatotropnogo gormona.

Somatogen, a human somatotropic hormone (HSH) prepared with the use of gene engineering (genHSH) does not differ from the highly purified substance of hypophysial hormone (hypHSH) in affinity to the antibodies produced on hypHSH and to the membrane receptor of the rabbit liver. 50 per cent inhibition of 125I hypHSH binding to the antibodies was observed at somatogen concentration of 8.2 ng/ml and hypHSH concentration of 7.9 ng/ml. The affinity constants of somatogen and hypHSH to the receptor amounted to 3.8 X 10(9) and 3.6 X 10(9) M-1, respectively. Somatogen for injections as a medicine contains 94.6 per cent of the hormone monomer and by the level of its homogeneity exceeds hypHSH as a medicine manufactured by Serano (Italy) and genHSH as a medicine manufactured by Kabi Vitrum (Sweden).

[Tolerance for prospidin ointment]. / K voprosu o perenosimosti prospidinovoi mazi.

The local and resorption action of 50% prospidin ointment administered at doses up to 100 mg/kg for a long time was studied in rabbit experiments. Prospidin ointment was established to exert no resorption action in doses tested. At the site of the ointment application there occur skin necrotic changes. Complete regeneration of the epithelium with hair recovery is recorded 2 months after the drug is discontinued.

[Mechanism of the pyrazidol potentiation of the hyperglycemic effect of adrenaline]. / K mekhanizmu usileniia pirazidolom giperglikemicheskogo éffekta adrenalina.

The effect of pyrazidol, a new tricyclic antidepressant, on the change in the activity of the phosphorylase in homogenates of the liver and heart, produced by epinephrine and cortisol, was studied. Information obtained in intact rats showed pyrazidol capable of potentiating the action of epinephrine, such effect being absent in adrenalectamized animals. The beta-adrenoblocking agent (propranolol) and the alpha-adrenoblocking agent (phentolamine) lower the activation of the phosphorylase called forth both by epinphrine and by the latter together with pyrazidol. In adrenalectomized rats phentolamine exerts against the background of the cortisol and pyrazidol action a more pronounced effect than does propranolol. These data suggest that one of the factors responsible for the potentiation of the antidepressant hyperglycemic action of epinephrine and cortisol is a greater intensity of glycogenolysis, coming as a result of the phosphorylase activation.

[Effect of pyrazidole on changes in blood sugar concentration caused by adrenaline and serotonin in intact and adrenalectomized animals]. / Vliianie pirazidola na izmenenie kontsentratsii sakhara krovi, vyzyvaemoe adrenalinom i serotoninom, u intaktnykh i adrenaléktomirovannykh zhivotnykh.

The effect produced by pyrazidol, a new tricyclic antidepressant, on changes in the blood sugar concentration caused by epinephrine and serotonin was investigated. Pyrazidol appreciably intensifies the effect of serotonin in intact and adrenalectomized animals. Under the influence of pyrazidol the action of epinephrine in intact animals also gains in strength, while in adrenalectomized animals pyrazidol lessens the hyperglycemic effect of epinephrine. Since the introduction of cortisol to adrenalectomized animals restores the effect of pyrazidol it is suggested that the influence of pyrazidol on the blood sugar concentration is accomplished with participation of glucocorticoids.

[Toxicological study of an antiviral drug, bonaphthon]. / Toksikologicheskoe izuchenie protivovirusnogo preparata bonaftona.

The toxicity of bonaphthon was studied on laboratory animals of different species (mice, rats, guinea pigs, dogs) with its single and repeated (daily, for a space of 4 months) administration. It has been found that bonaphthon, with its single and repeated administration in doses much higher (by 16 to 32 times) than the ones recommended for human beings, is well tolerated not only by sexually mature, but also by immature animals (rats). Bonaphthon does not affect adversely the dynamic changes in the weight of the animals, nor does it have any effect on the diuresis, functional state of the gastro-intestinal tract and the cardiac activity. On its administration in toxic doses exceeding by 60 and more timesthe ones recommened for human beings the drug provokes flabbiness, bradycardia, a fall of arterial pressure and causes changes in the activity of nonspecific enzymatic systems of the liver. Among the test animals most sensitive to bonaphthon are mice.