Prevention of Hepatorenal Insufficiency Associated with Lead Exposure by Hibiscus sabdariffa L. Beverages Using In Vivo Assay.

Lead pollution is a major environmental challenge worldwide. Therefore, dietary interventions that are aimed at preventing lead's deleterious effects on body organs are needed. The study's goal was to study and compare the protective effect of cold and hot beverages of Roselle (Hibiscus sabdariffa L.) red calyces (CRB and HRB, respectively) on liver and kidney insufficiency associated with lead exposure in male rats. Adult albino rats (32 males) were divided into four groups of equal number, including a normal control (group 1), while groups from 2 to 4 received lead acetate (20 mg/kg body weight/day) and were kept untreated (group 2). The 3rd and the 4th groups received CRB and HRB (0.5 ml/100 g body weight/day), respectively, for 6 weeks. The gain in the body and relative weights of the liver and kidneys were calculated. Liver and kidney functions were determined in serum, while lead, delta-aminolevulinic acid dehydratase, and oxidative stress markers were established in tissues. Specimens from the liver and kidney of sacrificed rats were histopathologically examined. The total activity of antioxidants and total content of anthocyanin of both beverages were determined. Lead exposure resulted in its accumulation in tissues, leading to overweight and liver and kidney insufficiency along with oxidative stress, which was further confirmed by histological staining. CRB was more efficient than HRB in preventing the deleterious effects of lead intoxication. Due to their antioxidant properties, the present study proved that Roselle red calyx beverages, particularly the cold ones, are protective agents against lead-associated disorders in a rat model.

Comparative ultrastructural-functional characterizations of the skin in three reptile species; Chalcides ocellatus, Uromastyx aegyptia aegyptia, and Psammophis schokari aegyptia (FORSKAL, 1775): Adaptive strategies to their habitat.

The current investigation was planned utilizing SEM, histological, and furthermore cytokeratin immunohistochemical to give a full depiction of skin of three reptiles species; Chalcides ocellatus (Scincidae), Uromastyx aegyptia aegyptia (Agamidae), and Psammophis schokari aegyptia (Colubridae) captured from Egypt with various ecological environment. Our SEM results showed different scales covered epidermis of three reptile's species with diverse surface microstructure. Overlapped rhomboid scales with numerous lenticular sense organs with numerous pores and oberhäutchen layer with microridges in C. ocellatus. In U. aegyptia, scales were overlapped elliptical-shaped possess lens-like sense structure with several scattered pits and oberhäutchen layer with polygonal outlined cells. While in P. schokari aegyptia, smooth scales flattened with two large dome-shaped scale receptors/sensilla and lens-like sense structure, moreover polygonal-shaped micro-ornamentation in scale hinge joints were observed. Histologically, skin of three species had outer epidermis with stratum germinativum, stratum corneum (α-keratin, ß-keratin layer) capped by surface Oberhäutchen and inner dermis. Osteoderms were observed with dermis of C. ocellatus. There are marked variation within pigment cells types among examined species. Melanophores observed in dermal layer of C. ocellatus, while in U. aegyptia, three pigment cells in tegument dermis; melanophores, xanthophores, and iridophores whereas, P. schokari aegyptia had two forms of chromatophore cells (melanophores and iridophores) in dermis and few melanophores scattered between stratum germinativum cells. The highest cytokeratin immunostaining observed in epidermal cell layer of U. aegyptia aegyptia than two other species. Conclusion, dry scaly skin of reptiles reflects a great range of functional aspects and success to adapt with terrestrial life.