ABSTRACT
OBJECTIVE: To understand practices of umbilical venous catheter (UVC) insertion in tertiary level neonatal intensive care units (NICU) and investigate the outcomes of subsequent attempts following a failed initial attempt. STUDY DESIGN: Prospective, multi-center observational study of UVC insertions at tertiary level NICUs between March 2019 and January 2020. RESULTS: Of the 101 UVCs inserted at 4 centers, seventy-two (71%) were central at the first attempt and 50% were central at subsequent attempts. Patients with at least 1 failed attempt at insertion were less likely to have a centrally placed UVC (p = .009). Manipulations were less likely to be required when UVC was centrally placed during the first attempt. Maneuvers such as posterior liver mobilization used during insertion were likely to be associated with successful central placement of UVC (p = .0243). The time to complete the procedure was significantly less when the UVC was central at the first attempt (34.2 ± 20.2 vs 46.9 ± 33.8) (p = .0292). Gestational age, birth weight, and age of the baby at the insertion of the UVC, experience of the provider, and type of catheter were comparable among groups. The Shukla formula was most commonly used by providers to measure the depth of UVC placement. CONCLUSION: Repetitive attempts and manipulations were less likely to be beneficial in the successful central placement of UVC in neonates. Additionally, repetitive attempts at insertion prolonged the overall duration of the procedure.
Subject(s)
Catheterization, Central Venous , Catheterization, Peripheral , Infant, Newborn , Humans , Catheterization, Peripheral/methods , Prospective Studies , Umbilical Veins , Birth Weight , CathetersABSTRACT
The long-term viability of a metapopulation depends partly on the gene flow among sub-populations. Management approaches such as translocations and supportive breeding between closely related populations may affect gene flow and overall structure, and therefore viability. Here, we examined temporal changes in the fine-scale population structure of coho salmon (Oncorhynchus kisutch) by comparing archived (1938) and modern (2001-2005) populations in six rivers within a single conservation unit (Puget Sound, Washington) sampled before and after an extended period of between-river transfers and releases of millions of cultured salmon. Genotype frequencies at eight microsatellite loci showed that current populations descended from historical Puget Sound populations, but populations in different rivers that exchanged fish for hatchery propagation share more of their ancestry recently than they did historically. Historically, populations in different rivers were isolated by geographic distance, but that relationship is no longer significant. Allelic richness among all populations declined significantly, suggesting that genetic drift has increased because of a population bottleneck. Populations in different rivers and within the same river have become more diverged, providing further evidence for a widespread bottleneck. Previously, we observed that genetic distance significantly decreased with the number of fish exchanged; however, some populations apparently resisted introgression. Altered gene flow and lost diversity may affect the complexity, and therefore resiliency of sub-populations within a conservation unit. Plans for artificial culture need to maintain existing genetic diversity and avoid disrupting the fine-scale structure by using local populations for parents whenever possible.
Subject(s)
Oncorhynchus kisutch/genetics , Animal Migration , Animals , Breeding , Evolution, Molecular , Female , Gene Flow , Genetic Variation , Male , Microsatellite Repeats , Oncorhynchus kisutch/physiology , Sexual Behavior, AnimalABSTRACT
We performed a genome scan at an average resolution of 8 cM in 719 Finnish sib pairs with type 2 diabetes. Our strongest results are for chromosome 20, where we observe a weighted maximum LOD score (MLS) of 2.15 at map position 69.5 cM from pter and secondary weighted LOD-score peaks of 2.04 at 56.5 cM and 1.99 at 17.5 cM. Our next largest MLS is for chromosome 11 (MLS = 1.75 at 84.0 cM), followed by chromosomes 2 (MLS = 0.87 at 5.5 cM), 10 (MLS = 0.77 at 75.0 cM), and 6 (MLS = 0.61 at 112.5 cM), all under an additive model. When we condition on chromosome 2 at 8.5 cM, the MLS for chromosome 20 increases to 5.50 at 69.0 cM (P=.0014). An ordered-subsets analysis based on families with high or low diabetes-related quantitative traits yielded results that support the possible existence of disease-predisposing genes on chromosomes 6 and 10. Genomewide linkage-disequilibrium analysis using microsatellite marker data revealed strong evidence of association for D22S423 (P=.00007). Further analyses are being carried out to confirm and to refine the location of these putative diabetes-predisposing genes.
Subject(s)
Chromosomes, Human/genetics , Diabetes Mellitus, Type 2/genetics , Genetic Predisposition to Disease/genetics , Aged , Chromosome Mapping , Diabetes Mellitus, Type 2/blood , Fasting , Female , Finland , Genome, Human , Humans , Linkage Disequilibrium/genetics , Lod Score , Male , Matched-Pair Analysis , Microsatellite Repeats/genetics , Middle Aged , Nuclear Family , Quantitative Trait, Heritable , United StatesABSTRACT
Type 2 diabetes mellitus is a complex disorder encompassing multiple metabolic defects. We report results from an autosomal genome scan for type 2 diabetes-related quantitative traits in 580 Finnish families ascertained for an affected sibling pair and analyzed by the variance components-based quantitative-trait locus (QTL) linkage approach. We analyzed diabetic and nondiabetic subjects separately, because of the possible impact of disease on the traits of interest. In diabetic individuals, our strongest results were observed on chromosomes 3 (fasting C-peptide/glucose: maximum LOD score [MLS] = 3.13 at 53.0 cM) and 13 (body-mass index: MLS = 3.28 at 5.0 cM). In nondiabetic individuals, the strongest results were observed on chromosomes 10 (acute insulin response: MLS = 3.11 at 21.0 cM), 13 (2-h insulin: MLS = 2.86 at 65.5 cM), and 17 (fasting insulin/glucose ratio: MLS = 3.20 at 9.0 cM). In several cases, there was evidence for overlapping signals between diabetic and nondiabetic individuals; therefore we performed joint analyses. In these joint analyses, we observed strong signals for chromosomes 3 (body-mass index: MLS = 3.43 at 59.5 cM), 17 (empirical insulin-resistance index: MLS = 3.61 at 0.0 cM), and 19 (empirical insulin-resistance index: MLS = 2.80 at 74.5 cM). Integrating genome-scan results from the companion article by Ghosh et al., we identify several regions that may harbor susceptibility genes for type 2 diabetes in the Finnish population.
Subject(s)
Diabetes Mellitus, Type 2/genetics , Genetic Testing , Genome, Human , Quantitative Trait, Heritable , Age Factors , Blood Glucose/metabolism , Body Mass Index , Chromosomes, Human/genetics , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/metabolism , Fasting , Female , Finland , Genetic Linkage/genetics , Genetic Predisposition to Disease/genetics , Humans , Insulin/blood , Male , Matched-Pair Analysis , Middle Aged , Nuclear Family , Sex Factors , United StatesABSTRACT
We are conducting a genome scan at an average resolution of 10 centimorgans (cM) for type 2 diabetes susceptibility genes in 716 affected sib pairs from 477 Finnish families. To date, our best evidence for linkage is on chromosome 20 with potentially separable peaks located on both the long and short arms. The unweighted multipoint maximum logarithm of odds score (MLS) was 3.08 on 20p (location, chi = 19.5 cM) under an additive model, whereas the weighted MLS was 2.06 on 20q (chi = 57 cM, recurrence risk,lambda(s) = 1. 25, P = 0.009). Weighted logarithm of odds scores of 2.00 (chi = 69.5 cM, P = 0.010) and 1.92 (chi = 18.5 cM, P = 0.013) were also observed. Ordered subset analyses based on sibships with extreme mean values of diabetes-related quantitative traits yielded sets of families who contributed disproportionately to the peaks. Two-hour glucose levels in offspring of diabetic individuals gave a MLS of 2. 12 (P = 0.0018) at 9.5 cM. Evidence from this and other studies suggests at least two diabetes-susceptibility genes on chromosome 20. We have also screened the gene for maturity-onset diabetes of the young 1, hepatic nuclear factor 4-a (HNF-4alpha) in 64 affected sibships with evidence for high chromosomal sharing at its location on chromosome 20q. We found no evidence that sequence changes in this gene accounted for the linkage results we observed.
Subject(s)
Chromosomes, Human, Pair 20 , Diabetes Mellitus, Type 2/genetics , Genetic Predisposition to Disease/genetics , Genetic Variation , Models, Genetic , Phosphoproteins/genetics , Transcription Factors/genetics , Adult , Basic Helix-Loop-Helix Leucine Zipper Transcription Factors , Blood Glucose/metabolism , Chromosome Mapping , DNA-Binding Proteins/genetics , Diabetes Mellitus, Type 2/blood , Exons , Female , Finland , Genetic Linkage , Genetic Markers , Glucose Tolerance Test , Hepatocyte Nuclear Factor 4 , Humans , Introns , Male , Middle Aged , Nuclear Family , Odds Ratio , Point Mutation , Polymorphism, Single-Stranded Conformational , Sequence Deletion , SpousesABSTRACT
In the first reported positive result from a genome scan for non-insulin-dependent diabetes mellitus (NIDDM), Hanis et al. found significant evidence of linkage for NIDDM on chromosome 2q37 and named the putative disease locus NIDDM1 (Hanis et al. 1996. Nat. Genet. 13:161-166). Their total sample was comprised of 440 Mexican-American affected sib-pairs from 246 sibships. The strongest evidence for linkage was at marker D2S125 and best estimates of lambdas (risk to siblings of probands/population prevalence) using this marker were 1.37 under an additive model and 1.36 under a multiplicative model. We examined this chromosomal region using linkage analysis in a Finnish sample comprised of 709 affected sib-pairs from 472 sibships. We excluded this region in our sample (multipoint logarithm of odds score = -2) for lambdas >/= 1.37. We discuss possible reasons why linkage to 2q37 was not found and conclude that this region is unlikely to be playing a major role in NIDDM susceptibility in the Finnish Caucasian population.
Subject(s)
Chromosomes, Human, Pair 2/genetics , Diabetes Mellitus, Type 2/genetics , Aged , Chromosome Mapping , Cohort Studies , Diabetes Mellitus, Type 2/epidemiology , Disease Susceptibility , Female , Finland/epidemiology , Genetic Markers , Genotype , Humans , Likelihood Functions , Lod Score , Male , Middle Aged , Nuclear Family , White People/geneticsABSTRACT
Large-scale genotyping is required to generate dense identity-by-descent maps to map genes for human complex disease. In some studies the number of genotypes needed can approach or even exceed 1 million. Generally, linkage and linkage disequilibrium analyses depend on clear allele identification and subsequent allele frequency estimation. Accurate grouping or categorization of each allele in the sample (allele calling or binning) is therefore an absolute requirement. Hence, a genotyping system that can reliably achieve this is necessary. In the case of affected sib-pair analysis without parents, the need for accurate allele calling is even more critical. We describe methods that permit precise sizing of alleles across multiple gels using the fluorescence-based, Applied Biosystems (ABI) genotyping technology and discuss ways to reduce genotyping error rates. Using database utilities, we show how to minimize intergel allele size variation, to combine data effectively from different models of ABI sequencing machines, and automatically bin alleles. The final data can then be converted into a format ready for analysis by statistical genetic packages such as MENDEL.
Subject(s)
Alleles , Blotting, Southern/methods , Chromosome Mapping/methods , Dinucleotide Repeats , Electrophoresis, Polyacrylamide Gel/methods , DNA/isolation & purification , DNA-Directed DNA Polymerase/genetics , Electronic Data Processing/methods , Genetic Linkage , Genetic Markers , Genetic Techniques , Genotype , Humans , Polymerase Chain Reaction , Quality Control , Taq PolymeraseSubject(s)
Platelet-Derived Growth Factor/metabolism , Receptors, Platelet-Derived Growth Factor/chemistry , Receptors, Platelet-Derived Growth Factor/metabolism , Animals , Baculoviridae , Binding Sites , Cloning, Molecular , Glycosylation , Humans , Kinetics , Ligands , Receptors, Platelet-Derived Growth Factor/biosynthesis , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Spodoptera , Time Factors , TransfectionSubject(s)
Athletic Injuries/psychology , Psychotherapy , Aging , Bone and Bones/injuries , Humans , Joints/injuries , Middle Aged , Narcissism , Self Concept , Social Adjustment , SymbolismABSTRACT
In an age of increasing reliance on scientific theory and technology, social work, as well as other behavioral science professions, has been challenged to utilize traditional scientific methodology for evaluation of counseling and direct practice services. A dilemma has arisen, however, because practitioners charge that the tools of traditional science lack conceptual relevance and practical utility within the practice setting. Analysis of selected research and counseling concepts, however, will reveal significant similarities between the two areas of practice and will demonstrate a way of teaching the integration of scientific and counseling ideas for the benefit of researchers and practitioners.
Subject(s)
Counseling , Quality of Health Care , Consumer Behavior , Humans , Outcome and Process Assessment, Health Care , ResearchABSTRACT
In a world with increasing demands for competent evaluation of counseling services, professional counselors are challenged to study and understand all facets of their unique methods of self-evaluation. This research report specifically discusses the type of communicated information used by counselors (N = 45) and suggests some improvements in self-evaluation based on the research results. The author discusses the use of behavioral, verbal cognitive and written communication because these types of information are used differentially to assess input or outcome of treatment. The sources of each form of information also are identified because this impacts the validity and reliability of self-evaluative decisions.