Fatal gunshot trauma of a child: A case from colonial Cyprus.

Forensic science has made some significant contributions to the investigation of human rights abuses related to armed conflicts, especially in the last 40 years. Some investigations are aimed at the collection of evidence in order to prosecute those responsible, while others are humanitarian in nature. This paper presents the multidisciplinary effort to recover and identify the remains of a 7-year-old child who was shot by British colonial forces in Cyprus in 1956. An investigation led to the discovery of the burial site, and archaeological methods were used to recover the remains. The anthropological examination provided information about the age of the child, as well as the nature of the skeletal trauma present. DNA results confirmed the identity of the victim, and the remains were released to the surviving family members for burial.

A proposed method for differentiating knives from cut marks on bone: A forensic anthropological approach.

Knife cut marks have been extensively studied in forensic literature, and the results have been beneficial in identifying both the cut mark and implement type on bone. For this study, an assessment in differentiating knife cut marks has been attempted by using sword cut mark characteristics. In addition, a flowchart was created to assist in interpreting such marks. Knives of different serrations (non-serrated, macro- and micro-serrated) were used to inflict cut marks on pig ribs with a forward and backward stab thrust. The analysis revealed that some traits adapted from previous studies applied to knife cut marks and that non-serrated and serrated marks could be distinguished accurately using the flowchart. The distinction between different types of serrated blades was not as successful. However, results show that further research and development of the flowchart can be invaluable in similar analyses.

Sex assessment from the pelvis: a test of the Phenice (1969) and Klales et al. (2012) methods.

Sex assessment is one of the first steps of routine forensic anthropological examinations and it provides a crucial element to identify a set of human skeletal remains. In bioarchaeological contexts, this assessment is also important, as it helps in the reconstruction of past societies. Sex determination can be achieved by using several morphological or metric traits of the skull and postcranial skeleton, which have been found to have varying degrees of accuracy. In 1969 Phenice proposed a methodology focusing on three traits located on the pubis. These traits were described as either having a female or male morphology with ambiguity being rare. Phenice's method became regularly utilized as it was considered to be reliable. In 2012, Klales and colleagues published a revision of Phenice's method, as they found that it did not capture the variation in the expression of the three traits. Klales and co-authors created a visual ordinal scale of 1-5 for each of the three traits Phenice originally identified, thus adding three extra possible forms of expression. The purpose of the present research was to test both the original and revised methodologies on the same skeletal population in order to evaluate their suitability for the assessment of sex. The Luís Lopes Anthropological collection in Lisbon was used; 117 males and 117 females were scored using both methodologies. The results showed that the original method performed better (96.5% accuracy) than the revised method (92.7%).

The detection of clandestine graves in an arid environment using thermal imaging deployed from an unmanned aerial vehicle.

The Middle East is one of the world regions that has frequently suffered from armed conflicts that resulted in mass burials. However, the detection of clandestine graves in such an arid environment by deploying remote sensing payload on unmanned aerial vehicles (UAVs) has received little attention. The present study used a UAV equipped with a thermal sensor aimed at narrowing down the search area of possible gravesites in the arid climate of Kuwait. The enclosed research area, which includes both control and experimental mass graves, was imaged for 18 months. The variation in topsoil temperature and soil moisture between the graves and their surroundings was evaluated. The results of the analysis demonstrated the effectiveness of thermal imaging techniques in detecting heat produced from buried sheep carcasses and detecting the change in grave soil moisture for our research environment for 7 and 10 months, respectively. The buried animals significantly influenced the topsoil temperature (p = 0.044), while the height from which the images were captured had an insignificant effect on the measured temperature within the range tested (p = 0.985). Furthermore, there was a negative correlation (-0.359) between grave temperature and the calculated soil moisture. The results from these cost- and time-effective search methods presented in this study confirm their potential for the detection of burial sites in an arid environment.

Evaluation of the obturator foramen as a sex assessment trait.

Correctly assessing sex from skeletal remains is one of the main elements of creating a biological profile. Many traits allow for this, the obturator foramen being one. However, research on its accuracy has provided mixed results. This study examines the obturator foramen using a 5-point grading scale to assess the degree of sexual dimorphism in four known age and sex skeletal collections from the UK and South Africa. Overall, sexual dimorphism was found in the obturator foramen when using the new scoring system; however, accuracies for correct sex classification ranged from ~ 46 to ~ 75%. Considering its wide range in accuracy rates across the four samples and difficulty in identifying the subtle changes in morphology, the obturator foramen should only be used as part of a multifactorial assessment of sex.

Biometric sex assessment from the femur and tibia in a modern Greek population.

Forensic anthropologists assess sex by analysing quantitative and qualitative characters of the human skeleton. In general, the pelvis and skull are the skeletal regions used most often, but in many cases, they are missing or fragmentary. In such circumstances, where only limb bones are present, it is necessary to use techniques based on other skeletal elements. Metric traits of the long bones of the lower extremities have been reported as reliable indicators of sex. This study was designed to determine whether the two main long bones of the leg, the femur and tibia, can be used for the assessment of sex on a Greek skeletal population. The skeletal sample used in this study comes from the modern human skeletal collection that is currently housed at the National and Kapodistrian University of Athens and is known as The Athens Collection. It consists of 371 femora and 372 tibiae corresponding to 200 adult individuals (111 males and 89 females). The age range is 19-96 years for males and 20-99 years for females. The maximum lengths and epiphyseal widths were measured for the present study, and it was found that the discriminant analysis of the metrical data of each long bone provided high sex discrimination accuracies. The rate of correct sex discrimination based on different long bones ranged from 91.50 % (left femur) to 93.40 % (left tibia). Our results suggest that lower limb bones can be used effectively for sexing in forensic contexts, in addition to other sex assessment techniques.

Technical note: Development of regression equations to reassociate upper limb bones from commingled contexts.

The major upper limb skeletal elements (scapulae, humeri, ulnae and radii) are frequently utilized for sex determination and stature estimation. Consequently, in forensic cases that involve commingled remains, it is crucial to reassociate the aforementioned bones and attribute them to the right individual. The aim of the present study is to develop simple and multiple regression equations for sorting commingled human skeletal elements of the upper limb. In that context, ten common anthropological linear measurements of the articular surfaces of scapulae, humeri, ulnae, and radii were performed on 222 adult skeletons from the Athens Collection. The functions developed for sorting adjoining bones presented a strong positive linear relationship (r=0.69-0.93, p<0.05). The values of the determination coefficient statistics (r2=0.47-0.86) were found to be high and those of the standard errors of the estimate were found to be low (SEE=0.88-1.61). Blind tests indicated that when metric and morphoscopic sorting techniques are combined, a reliable sorting of the skeletal elements of the upper limbs is possible.

Septal Aperture of the Humerus: Etiology and Frequency Rates in Two European Populations.

Analysis of the septal aperture was conducted on two documented European populations. Collections from the National Museum of Natural History Lisbon, Portugal, and University of Athens, Greece, were used for the study. Both collections are modern and documented for sex and age. The Portuguese sample comprises 297 individuals (149 males and 148 females) between the ages of 18 and 88. A septal aperture was observed in 50 individuals resulting in a frequency of 16.83%. The Greek sample comprises 117 individuals (68 males and 49 females) between the ages of 20 and 65. Twenty-five septal apertures were observed, giving a frequency of 21.37%. Both populations had high frequencies which exceeded those observed in European countries in previous studies. Sex analysis shows that both samples confirm that septal apertures are more common in females. The Portuguese sample also supports that septal apertures are more common in the left humerus; however, the Greek sample had a higher frequency of bilateral cases. Measurements of the Portuguese sample were taken to determine whether robusticity correlates with presence of septal apertures. These measurements concluded that there was no difference in robusticity with presence or absence of a septal aperture, challenging previous studies. Anat Rec, 2019. © 2019 American Association for Anatomy Anat Rec, 303:1821-1830, 2020. © 2019 American Association for Anatomy.

Biometric sex estimation using the scapula and clavicle in a modern Greek population.

ABSTRACT: Human skeletal remains exhibit sexual dimorphism, which is apparent in adult living individuals as well. Researchers from forensic and osteoarchaeological fields have a growing interest in establishing metric standards for sexing by use of discriminant function analysis. The present study focuses on providing sex estimation metric standards using measurements from the scapula and clavicle in a Modern Greek sample (Athens Collection) consisting of 107 male and 90 female skeletons. A total of seven measurements were taken: maximum scapular height and breadth, glenoid cavity height and breadth, maximum clavicular length, anterior and superior clavicular diameter. The calculation of Sexual Dimorphism Index showed a significant degree of sexual dimorphism on both bones ranging from 10% to 18%. Statistical analysis provided the discriminant functions with an accuracy of correct sex estimation between 84.9% and 91.4%, and showed that the highest accuracy rate was obtained from the left scapula measurements; proving that this bone can be a useful tool in providing an accurate sex estimation in skeletal remains of Modern Greek origin.

Sharp Force Trauma Death in a Young Individual From Medieval Gloucester.

The authors of the present work evaluate the trauma observed on the skeletal remains of an individual from medieval Gloucester and reconstruct the events that led to his death. The almost complete skeleton was recovered from the cemetery of St Owen and dates to the late medieval period. Several methods were used to determine the sex and age of the individual. The anthropological examination showed that the remains belonged to a young male, between the ages of 17 and 19 years. The young man also had antemortem pathologies that were related to his diet and lifestyle, as he appears to have had iron-deficiency anemia and Schmorl nodes. The trauma observed on the remains consisted of 3 cut marks located on the cranium, left radius, and right scapula. The cuts seem to have been inflicted by a heavy weapon, such as a sword. The trauma pattern observed is consistent with defensive action, and the fact that this skeleton was the only one in the collection that has evidence of trauma suggests that this was a case of interpersonal violence.

Assessing size and strength of the clavicle for its usefulness for sex estimation in a British medieval sample.

The construction of the biological profile from human skeletal remains is the foundation of anthropological examination. However, remains may be fragmentary and the elements usually employed, such as the pelvis and skull, are not available. The clavicle has been successfully used for sex estimation in samples from Iran and Greece. In the present study, the aim was to test the suitability of the measurements used in those previous studies on a British Medieval population. In addition, the project tested whether discrimination between sexes was due to size or clavicular strength. The sample consisted of 23 females and 25 males of pre-determined sex from two medieval collections: Poulton and Gloucester. Six measurements were taken using an osteometric board, sliding calipers and graduated tape. In addition, putty rings and bi-planar radiographs were made and robusticity measures calculated. The resulting variables were used in stepwise discriminant analyses. The linear measurements allowed correct sex classification in 89.6% of all individuals. This demonstrates the applicability of the clavicle for sex estimation in British populations. The most powerful discriminant factor was maximum clavicular length and the best combination of factors was maximum clavicular length and circumference. This result is similar to that obtained by other studies. To further investigate the extent of sexual dimorphism of the clavicle, the biomechanical properties of the polar second moment of area J and the ratio of maximum to minimum bending rigidity are included in the analysis. These were found to have little influence when entered into the discriminant function analysis.

Application of Purkait's triangle method on a skeletal population from southern Europe.

The existence of sexual dimorphism in individual elements of the human skeleton allows sex determination and significantly enhances the identification of fragmentary skeletal material. Traditionally, the pelvis and the skull are used for an accurate sex determination. Sometimes they are not available and alternative methods have to be used. The aim of this work is to study the sexual dimorphism of the proximal portion of the posterior femur by analyzing the biometric data of the Purkait's triangle. The studied sample comprises 203 individuals (112 males and 91 females) from the Modern Human Skeletal Reference Collection (The Athens Collection) of the Department of Animal & Human Physiology (National & Kapodistrian University of Athens). The biometric data were analyzed by Discriminant Analysis and equations were generated for sex determination, which gave an overall correct classification of 78.3%. For the right and the left femur the percentages of correct classification were 77.8% and 75.9% respectively. These accuracy rates were compared to those of an Indian population and were found to be lower (86.5% for males and 86.3% for females). The study of sexual dimorphism (SD) in Greek, Indian, European-American and African-American populations gave interesting results. The AB diameter in the Greek sample shows the lowest degree of SD while the AC diameter shows the highest. In the other three population samples, the BC diameter shows the highest degree of SD. We assume that the femur anatomy of the Greeks is the major cause that the Purkait method does not give high rates of correct classification. It is therefore advisable that practitioners exercise caution when using this method on skeletal material from Greece, especially in forensic contexts.

A test of the revised auricular surface aging method on a modern European population.

The accurate age estimation of adults is an important step in the construction of the biological profile of skeletonized remains. The auricular surface of the ilium as it was developed in 1985 by Lovejoy et al., is one of the methods employed for age estimation. This study presents the results of a blind test of the revised auricular surface aging method developed by Buckberry and Chamberlain. A sample of 120 individuals from the Athens Collection was used to test this revised aging technique. Almost all features and composite score were positively correlated with known age-at-death. The calculation of bias demonstrated no obvious trend for either overestimation or underestimation of age when all individuals were pooled together. Inaccuracy showed that absolute errors of estimated ages against known ages are substantial. The data generated from this study suggest that the revised method can be reliable for age estimation on a modern European population.

Sex determination in modern Greeks using diagonal measurements of molar teeth.

Sex determination is a necessary step in the investigation of unidentified human remains from a forensic context. Teeth, as one of the strongest tissues in the human body, can be used for this purpose. Most studies of sexual dimorphism in teeth are based on the traditional mesiodistal and buccolingual crown measurements. The purpose of this study is to examine the degree of sexual dimorphism in permanent molars of modern Greeks using crown and cervical diagonal diameters, and to evaluate their applicability in sex determination. A total of 344 permanent molars in 107 individuals (53 male and 54 female) from the Athens Collection were examined. Crown and cervical diagonal diameters of both maxillary and mandibular molars were measured. It was found that males have larger molars than females and in 19 out of 24 dimensions measured male molars exceeded female molars significantly (P<0.05). The most dimorphic molars are the maxillary second molar, and the mandibular second and first molars. Although other molars were also sexually dimorphic they did not have a statistically significant difference in all dimensions. Cervical diagonal diameters have found to be more sexually diamorphic than crown diagonal diameters. In discriminant function analysis the variables entered more frequently were the cervical diagonal diameters mainly of mandibular molars. Classification accuracy was found to be 93% for the total sample, 77.4% for upper jaw, and 88.4% for the lower jaw. Accuracy rates were higher for cervical than crown diagonal diameters. The data generated from the present study suggest that this metric method can be useful and reliable for sex determination, especially when the traditional dental measurements are not applicable.

Guidelines for the recognition of cemetery remains in Greece.

Forensic pathologists frequently consult anthropologists for the identification of skeletonized human remains. These remains may be the result of criminal activity or remains that were unearthed because of erosion, or during construction projects. In some cases, human remains that had been previously buried in a cemetery may be the subject of a forensic investigation. Early recognition of cemetery remains prevents unnecessary efforts and conserves precious resources. One of the key characteristics of cemetery remains is the presence of embalmed tissue. However, there are countries where embalming is not a common practice, and other clues must be sought for identifying previously buried remains. Current funerary customs in Greece and, in particular, the tradition of exhumations result in a large number of misplaced human remains. The present study presents examples of cemetery remains from Greece and offers guidelines for recognizing changes on skeletal remains that may be indicative of a cemetery origin. Location of discovery, condition of the remains, and the types of associated artifacts are all factors that aid forensic anthropologists in identifying cemetery remains.

A simple technique for imaging the human skeleton using a flatbed scanner.

A simple technique for imaging the human skeleton with a flatbed scanner is presented using the auricular surface of the ilium as an example. A flatbed scanner with resolution capabilities of 600 dpi or greater allows for images of human bones. The auricular surface of the ilium was selected to demonstrate this technique as it is a fairly three-dimensional area that can be difficult to record photographically. Fifty left ilia of various ages at death from the Athens Collection were selected from which three observers (SCF, CE, and IM) scored the morphology of the auricular surface using a well-established aging method. Observations were taken of the dry bone, of digital photographs of the bone, and of scanned images of the bone, and in that sequence. Results indicate that scores of scanned images are equivalent or better than digital images of the same ilia. This technique allows for sharing data electronically with ease.

Sexual dimorphism of the arm bones in a modern greek population.

Several studies have shown that sex determination methods based on measurements of the skeleton are population specific. Metric traits of the long bones of the arm have been reported as reliable indicators of sex. This study was designed to determine whether the three long bones of the arm can be used for sex determination on a skeletal population from Greece. The material used consists of the arm bones of 204 adult individuals (111 males and 93 females) coming from the Modern Human Skeletal Collection of the University of Athens. The age range is 19-96 years for males and 20-99 years for females. The maximum lengths and epiphyseal widths were measured in the long bones of the arm (humerus, radius, and ulna). The discriminant analysis of the metrical data of each long bone gave very high discrimination accuracies. The rate of correct sex discrimination based on different long bones ranges from 90.30% (ulna) to 95.70% (humerus). In addition, intra- and inter-observer error tests were performed. These indicated that replication of measurements was satisfactory for the same observer over time and between observers. The results of this study show that metric characteristics of the arm bones can be used for the determination of sex in skeletal remains from Greece and that bone dimensions are population specific.

Sex determination using metatarsal osteometrics from the Athens collection.

The determination of sex in skeletal remains performed by forensic anthropologists or bioarchaeologists typically relies on the analysis of quantitative and qualitative characteristics of the skeleton. In this regard, the most widely used features belong to the pelvic and cranial areas, but these are often not available in forensic or archaeological contexts. The objective of this study is to determine the extent of dimorphism exhibited by the metatarsal bones in order to examine their utility in the metric determination of sex in skeletal remains of Greek origin. A further objective is the development of linear discriminant function equations for sex determination based on the metatarsals from a contemporary Greek population. The skeletal sample used in this study is comprised of 1595 metatarsals (left and right), corresponding to 186 adult individuals (97 males, 89 females) and belongs to the documented collection of the University of Athens. The results suggest that metatarsal bones exhibit significant sexual dimorphism and the accuracy of the discriminant function equations for sex determination range from 80.7% to 90.1% (or 77.9-86.4% cross-validated). Thus, metatarsal bones may be used for sex determination in skeletal remains from Greece in addition to other sexing techniques.

Sex determination using metacarpal biometric data from the Athens Collection.

Forensic anthropologists and bioarchaeologists usually determine sex by analyzing quantitative and qualitative characters of the human skeleton. In general, the pelvis and skull are the skeletal parts used most often, but in many cases these parts are missing or fragmentary. In such circumstances, it is necessary to use techniques based on other skeletal elements. The primary aim of this work is to determine whether metacarpals can be used for the determination of sex specifically in a Greek population. A secondary step is to describe and develop linear discriminant function equations for sex determination based on a contemporary Greek population using metacarpal biometric data. The skeletal sample of the modern Greeks is housed at the National and Kapodistrian University of Athens (NKUA) is comprised of 993 metacarpals (left and right), corresponding to 151 adult individuals (84 males and 67 females). The correct sex classification reached an accuracy of 83.7-88.1% for left and 83.8-89.7%, for right metacarpals. Our results suggest that metacarpals can be used for sexing in bioarchaeological, and forensic studies, in addition to other sex determination techniques.