Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 6 de 6
Filter
Add more filters










Language
Publication year range
1.
Rev Bras Parasitol Vet ; 22(1): 92-7, 2013.
Article in English | MEDLINE | ID: mdl-23538502

ABSTRACT

Dogs are definite hosts for several zoonotic helminthes and protozoan. Rural areas from the Lobos District in the northeast of Buenos Aires province, Argentina, are mainly used for livestock activity, increasing in this way the number of dogs on farms as well as the human risk of parasitic infections. The aims of this research were to evaluate the endoparasitic infections in dogs from farms in the Lobos District and analyze their zoonotic importance as well as several risk practices and habits of the rural population. Forty-two dog fecal samples obtained in 21 farms were analyzed through coproparasitological methods and coproantigen tests, which resulted in an overall parasite prevalence of 69.05% and 80.95% of the parasitized farms. The most frequent parasites were Trichuris vulpis and Eucoleus aerophila (26.19%), Echinococcus granulosus (19.05%), Uncinaria stenocephala and coccids (14.29%). The analysis of epidemiological files showed several habits of the rural population considered as risk factors associated with the presence of fecal samples parasitized and the presence of E. granulosus on the farms. It is clear that people involved with the farms studied were exposed to several helminthes that could cause serious diseases like cystic echinococcosis, which can become an important public health issue and affect the economy worldwide.


Subject(s)
Dog Diseases/epidemiology , Dog Diseases/parasitology , Animals , Argentina/epidemiology , Dogs , Feces/parasitology , Rural Health , Zoonoses
2.
Rev. bras. parasitol. vet ; 22(1): 92-97, Jan.-Mar. 2013. tab
Article in English | LILACS, VETINDEX | ID: lil-671615

ABSTRACT

Dogs are definite hosts for several zoonotic helminthes and protozoan. Rural areas from the Lobos District in the northeast of Buenos Aires province, Argentina, are mainly used for livestock activity, increasing in this way the number of dogs on farms as well as the human risk of parasitic infections. The aims of this research were to evaluate the endoparasitic infections in dogs from farms in the Lobos District and analyze their zoonotic importance as well as several risk practices and habits of the rural population. Forty-two dog fecal samples obtained in 21 farms were analyzed through coproparasitological methods and coproantigen tests, which resulted in an overall parasite prevalence of 69.05% and 80.95% of the parasitized farms. The most frequent parasites were Trichuris vulpis and Eucoleus aerophila (26.19%), Echinococcus granulosus (19.05%), Uncinaria stenocephala and coccids (14.29%). The analysis of epidemiological files showed several habits of the rural population considered as risk factors associated with the presence of fecal samples parasitized and the presence of E. granulosus on the farms. It is clear that people involved with the farms studied were exposed to several helminthes that could cause serious diseases like cystic echinococcosis, which can become an important public health issue and affect the economy worldwide.


Os cães são hospedeiros definitivos de vários helmintos e protozoários zoonóticos. As áreas rurais do distrito de Lobos, no nordeste da província de Buenos Aires, Argentina, destinam-se principalmente à atividade pecuária aumentando desta forma o número de cães em fazendas, bem como o risco humano de infecções parasitárias. Os objetivos desta pesquisa foram avaliar as infecções endoparasitas em cães de fazendas do distrito de Lobos e analisar a sua importância zoonótica, bem como as práticas de risco e hábitos da população rural. Quarenta e duas amostras de fezes de cães obtidos em 21 fazendas foram analisadas pelos métodos coproparasitológicos e testes de coproantígenos, resultando numa prevalência de parasitas de 69,05% e 80,95% das propriedades parasitados. Os parasitas mais frequentes foram Trichuris vulpis e Eucoleus aerophila (26,19%), Echinococcus granulosus (19,05%), Uncinaria stenocephala e coccídeos (14,29%). A análise dos arquivos epidemiológicos mostraram vários hábitos da população rural como fatores de risco associados com a presença de amostras fecais parasitadas e a presença de E. granulosus em propriedades rurais. É evidente que as pessoas das fazendas estudadas foram expostas a vários helmintos que podem causar doenças graves como equinococose cística, que constitui importante problema de saúde pública e econômico a nível mundial.


Subject(s)
Animals , Dogs , Echinococcus granulosus , Echinococcosis/epidemiology , Dog Diseases/epidemiology , Dog Diseases/parasitology , Feces/parasitology , Argentina/epidemiology , Rural Health , Zoonoses
3.
Antimicrob Agents Chemother ; 55(12): 5861-7, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21930885

ABSTRACT

The need to identify improved therapy against cystic echinococcosis (CE) has motivated pharmacology-based research. The comparative pharmacological performances of the benzimidazole compounds flubendazole (FLBZ) and albendazole (ABZ) were addressed here. The goals of the work were as follows: (i) to evaluate the ex vivo activities of FLBZ, ABZ, and their respective metabolites against Echinococcus granulosus protoscoleces, (ii) to compare the plasma and cyst disposition kinetics for the two drugs in infected mice, and (iii) to compare the clinical efficacies of FLBZ and ABZ against CE in mice. For the ex vivo study, E. granulosus protoscoleces were incubated with FLBZ, reduced FLBZ (R-FLBZ), ABZ, and ABZ-sulfoxide (ABZSO) (10 nmol/ml). Protoscolex viability was monitored by the methylene blue exclusion test and scanning electron microscopy (SEM). For the pharmacokinetic study, BALB/c mice with CE were allocated to two different groups and orally treated with either FLBZ or ABZ (5 mg/kg of body weight), both formulated as a cyclodextrin-based solution. Blood and cyst samples were taken up to 12 h posttreatment and analyzed by high-performance liquid chromatography (HPLC). For the efficacy study, CE-infected BALB/c mice were divided into three groups: the unmedicated control group and the FLBZ- and ABZ-treated groups. Oral treatments were performed twice a day during 25 days. After treatment, all animals were killed and the weight of the cysts was recorded. Loss of protoscolex viability was observed after drug incubation. FLBZ was detected in plasma (area under the concentration-versus-time curve [AUC] = 1.8 µg · h/ml) and cysts (AUC = 0.3 µg · h/g) collected from treated infected animals. Conversely, ABZSO was the only active molecule measured in plasma (AUC = 4.4 µg·h/ml) and cysts (AUC = 1.5 µg·h/g) after ABZ treatment. FLBZ induced a 90% reduction in cyst weight in comparison to those collected from untreated control mice (P < 0.05). However, no differences in cyst weight were observed between the ABZ-treated (8.2 g) and unmedicated control (10.5 g) groups. Due to these results, we consider flubendazole to have great potential to become a drug of choice in the treatment of cystic echinococcosis.


Subject(s)
Albendazole , Anticestodal Agents , Echinococcosis/drug therapy , Echinococcus granulosus/drug effects , Mebendazole/analogs & derivatives , Albendazole/administration & dosage , Albendazole/pharmacokinetics , Albendazole/pharmacology , Albendazole/therapeutic use , Animals , Anticestodal Agents/administration & dosage , Anticestodal Agents/pharmacokinetics , Anticestodal Agents/pharmacology , Anticestodal Agents/therapeutic use , Echinococcosis/metabolism , Echinococcosis/parasitology , Echinococcus granulosus/growth & development , Echinococcus granulosus/ultrastructure , Mebendazole/administration & dosage , Mebendazole/pharmacokinetics , Mebendazole/pharmacology , Mebendazole/therapeutic use , Mice , Mice, Inbred BALB C , Treatment Outcome
4.
Chemotherapy ; 56(5): 386-92, 2010.
Article in English | MEDLINE | ID: mdl-20948209

ABSTRACT

BACKGROUND: Cystic echinococcosis (CE) is an important public health problem worldwide. Flubendazole, administered in tablets, has shown poor in vivo efficacy against CE in humans. However, flubendazole prepared as a solution caused a marked reduction in hydatid cysts developed in mice. The goal of the current work was to compare the chemoprophylactic effect of flubendazole formulated either as a hydroxypropyl-ß-cyclodextrin solution or as a carboxymethylcellulose suspension in secondary CE in mice. METHODS: Balb/C mice were infected with Echinococcus granulosus protoscoleces. One day after infection, the animals were allocated into 3 different experimental groups: unmedicated control and treated at the time point of infection with flubendazole either prepared as a solution or suspension given twice a day during 15 days. Six months after infection, the animals were sacrificed to collect and weight parasitic cysts. Cyst samples recovered from infected mice of each experimental group were prepared for both scanning and transmission electron microscopy. RESULTS: Both flubendazole formulations induced a significant reduction in cyst weight compared to the cysts recovered from the unmedicated control animals. Both formulations showed similar flubendazole-induced ultrastructural morphological changes. CONCLUSION: Flubendazole offers a great potential to become a drug of choice in the preventive treatment of cystic echinococcosis.


Subject(s)
Antinematodal Agents/therapeutic use , Echinococcosis/prevention & control , Mebendazole/analogs & derivatives , 2-Hydroxypropyl-beta-cyclodextrin , Animals , Carboxymethylcellulose Sodium/chemistry , Chemistry, Pharmaceutical , Disease Models, Animal , Echinococcosis/pathology , Mebendazole/therapeutic use , Mice , Mice, Inbred BALB C , Microscopy, Electron, Scanning , Microscopy, Electron, Transmission , beta-Cyclodextrins/chemistry
5.
Parasitol Res ; 103(2): 355-62, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18465143

ABSTRACT

The pharmacokinetic (PK) behaviour and clinical efficacy of albendazole (ABZ) against hydatid cysts in mice were assessed after treatment with two different ABZ pharmaceutical formulations. BalbC mice received ABZ (0.5 mg/kg) prepared either as solution or suspension (50 microg/ml) for oral administration (PK study). Blood samples were collected up to 16 h post-treatment and processed to measure ABZ/metabolites concentrations in plasma. The clinical efficacy assessment was performed in BalbC mice infected 8 months earlier with Echinococcus granulosus protoscoleces. Infected animals were allocated into three experimental treatment groups: (a) untreated control, (b) ABZ-solution treated, (c) ABZ-suspension treated. Both treated groups received ABZ (0.5 mg/kg) administered under two different therapeutic schemes: dosing every 48 h over 30 days (regimen I) or treated every 12 h during 15 days (regimen II). Experimental mice were sacrificed 12 h after treatment, and cysts were recovered, weighed and processed for transmission electron microscopy. Enhanced ABZ sulphoxide (the main ABZ metabolite) concentration profiles were measured in animals treated with the ABZ solution. Any positive clinical response was obtained after treatment every 48 h (30 days therapy). However, consistent with the observed PK results, both ABZ formulations were clinically effective in infected mice treated with a 12-h dosing interval (15 days therapy).


Subject(s)
Albendazole/pharmacokinetics , Albendazole/therapeutic use , Anthelmintics/pharmacokinetics , Anthelmintics/therapeutic use , Echinococcosis/drug therapy , Echinococcus granulosus , Albendazole/administration & dosage , Animals , Anthelmintics/administration & dosage , Chemistry, Pharmaceutical , Disease Models, Animal , Drug Administration Schedule , Echinococcosis/parasitology , Echinococcus granulosus/drug effects , Echinococcus granulosus/ultrastructure , Mice , Mice, Inbred BALB C , Microscopy, Electron, Transmission , Pharmaceutical Solutions , Treatment Outcome
6.
Vet Parasitol ; 138(3-4): 377-81, 2006 Jun 15.
Article in English | MEDLINE | ID: mdl-16504405

ABSTRACT

Cystic echinococcosis is the most prevalent zoonosis in Tierra del Fuego province, Argentina, with important economic, productive and public health consequences. The present work was performed to determine the ovine prevalence in Tierra del Fuego, Argentina, as well as to evaluate the quality of diagnostic systems in slaughterhouses. Moreover, genetic analyses to characterize the strain of Echinococcus granulosus involved in the region were done. The first actions to perform a diagnosis of the epidemiological situation of hydatidosis in Tierra del Fuego were done between 1976 and 1977. A canine prevalence of 80% and an ovine prevalence of 55% results were obtained. Since 1979 the control program of Hydatidosis of Tierra del Fuego was implemented. It was based on semiannual canine anthelmintic treatment with praziquantel at dose of 5mg/kg, and complemented with sanitary education and canine and ovine epidemiological surveillance. During May 1997-January 1999: 5,916 sheep coming from 20 farms of the programmatic area were evaluated. In the lamb category, hydatid cysts were not found. In the adults category, 62 infected animals were found (3.2%). The ovine prevalence was 1.1% and there was 100% of coincidence between diagnosis in the slaughterhouse, re-inspection in the laboratory and histopathological study. The marked decrease in the prevalence observed for sheep infection evidenced a destabilization of the biological cycle of the parasite. This could be explained by the application of a control program with uninterrupted systematic actions. Polymerase chain reaction-ribosomal ITS-1 DNA (rDNA) restriction fragment length polymorphism (PCR-RFLP) analysis and partial sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene were used to characterize E. granulosus isolates collected from different regions of Tierra del Fuego to determine which genotypes occurred in this region. The results revealed the presence of the G1 genotype (sheep-dog strain). This is the first time that a molecular analysis was performed for the E. granulosus isolates from Tierra del Fuego.


Subject(s)
Echinococcosis/veterinary , Echinococcus granulosus/classification , Sheep Diseases/epidemiology , Abattoirs , Animals , Argentina/epidemiology , DNA, Mitochondrial/chemistry , DNA, Ribosomal Spacer/genetics , Dogs , Echinococcosis/diagnosis , Echinococcosis/epidemiology , Echinococcus granulosus/genetics , Echinococcus granulosus/isolation & purification , Electron Transport Complex IV/genetics , Humans , Liver/parasitology , Lung/parasitology , Polymerase Chain Reaction/veterinary , Polymorphism, Restriction Fragment Length , Prevalence , Sheep , Sheep Diseases/diagnosis , Sheep Diseases/parasitology , Zoonoses
SELECTION OF CITATIONS
SEARCH DETAIL