ABSTRACT
An isotachophoretic method is presented for the analysis of the glycoprotein mucin as raw material. The assay is also used for the mucin assay in the saliva substitute Saliva Orthana.
Subject(s)
Mouthwashes/analysis , Mucins/analysis , Saliva, Artificial/analysis , Animals , Electrophoresis , Indicators and Reagents , Spectrophotometry, Ultraviolet , SwineABSTRACT
Cisplatin in normal saline of pH 2.5 caused haemolysis of rat erythrocytes, whereas cisplatin in normal saline of pH 3.5 did not. Even a difference of 0.2 pH units appeared to be of significant importance: haemolysis of rat erythrocytes was observed with cisplatin in saline of pH 3.0 but not with cisplatin in saline of pH 3.2. The LD in mice was 15.4 mg/kg for cisplatin in saline of pH 2.5 versus 24.0 mg/kg for cisplatin in saline of pH 3.5. Experiments with cisplatin should include careful control of pH.
Subject(s)
Cisplatin/toxicity , Erythrocytes/drug effects , Animals , Hemolysis/drug effects , Hydrogen-Ion Concentration , In Vitro Techniques , Lethal Dose 50 , Male , Rats , Rats, Inbred StrainsABSTRACT
This study shows that peritoneal dialysis of theophylline-intoxicated rats results in withdrawal of approximately 27% of the administered dose of theophylline in 4 hr. Clinical consequences of this experience are considered.