ABSTRACT
The aim of the present study is to evaluate the outcome of hand-sewn esophagogastric anastomosis during radical esophagectomy for esophageal cancer. The outcomes of 467 consecutive esophageal cancer patients who underwent cervical esophagogastric anastomosis using interrupted and double-layered sutures after radical esophagectomy via right thoracotomy or thoracoscopic surgery were retrospectively reviewed. Anastomotic leakage, including conduit necrosis, occurred in 11 of 467 patients (2.4%); 7 of 11 (63.6%) cases experienced only minor leakage, whereas the other four (36.4%) patients had major leakage that required surgical or radiologic intervention, including two patients of conduit necrosis. Anastomotic leakages were more frequently observed after retrosternal reconstruction compared with the posterior mediastinal route (P < 0.0001). The median time to healing of leakage was 40 days (range: 14-97 days). Two patients (2/467, 0.4%) died in the hospital due to sepsis caused by the leakage and conduit necrosis. Twelve patients (2.6%) developed anastomotic stenosis, which was improved by dilatation in all patients. Hand-sewn cervical esophagogastric anastomosis is a stable and highly safe method of radical esophagectomy for esophageal cancer.
Subject(s)
Anastomotic Leak/epidemiology , Esophageal Neoplasms/surgery , Esophagectomy/methods , Esophagostomy/methods , Esophagus/surgery , Aged , Anastomosis, Surgical/adverse effects , Anastomosis, Surgical/methods , Anastomotic Leak/etiology , Esophagostomy/adverse effects , Esophagus/pathology , Female , Humans , Male , Middle Aged , Retrospective Studies , Treatment OutcomeABSTRACT
Genotyping graft livers by short tandem repeats after human living-donor liver transplantation (n = 20) revealed the presence of recipient or chimeric genotype cases in hepatocytes (6 of 17, 35.3%), sinusoidal cells (18 of 18, 100%), cholangiocytes (15 of 17, 88.2%) and cells in the periportal areas (7 of 8, 87.5%), suggesting extrahepatic cell involvement in liver regeneration. Regarding extrahepatic origin, bone marrow mesenchymal stem cells (BM-MSCs) have been suggested to contribute to liver regeneration but compose a heterogeneous population. We focused on a more specific subpopulation (1-2% of BM-MSCs), called multilineage-differentiating stress-enduring (Muse) cells, for their ability to differentiate into liver-lineage cells and repair tissue. We generated a physical partial hepatectomy model in immunodeficient mice and injected green fluorescent protein (GFP)-labeled human BM-MSC Muse cells intravenously (n = 20). Immunohistochemistry, fluorescence in situ hybridization and species-specific polymerase chain reaction revealed that they integrated into regenerating areas and expressed liver progenitor markers during the early phase and then differentiated spontaneously into major liver components, including hepatocytes (≈74.3% of GFP-positive integrated Muse cells), cholangiocytes (≈17.7%), sinusoidal endothelial cells (≈2.0%), and Kupffer cells (≈6.0%). In contrast, the remaining cells in the BM-MSCs were not detected in the liver for up to 4 weeks. These results suggest that Muse cells are the predominant population of BM-MSCs that are capable of replacing major liver components during liver regeneration.
Subject(s)
Bone Marrow Transplantation , Liver Diseases/surgery , Liver Regeneration/physiology , Mesenchymal Stem Cell Transplantation , Postoperative Complications/therapy , Adult , Animals , Child , Female , Humans , Immunoenzyme Techniques , In Situ Hybridization, Fluorescence , Liver Transplantation/adverse effects , Male , Mice , Mice, Inbred ICR , Mice, SCID , PrognosisABSTRACT
We assessed the association between obesity measurements including visceral adipose tissue (VAT), measured by computed tomography, and the risk of high-grade prostate cancer after radical prostatectomy. We investigated 296 patients who were diagnosed with prostate cancer and underwent radical prostatectomy. Data extracted from medical records included age, body mass index (BMI), VAT, pretreatment prostate-specific antigen (PSA) levels and Gleason score (GS). We performed logistic regression to examine the association between indicators of obesity and a higher GS (⩾4+3). Among the 296 patients, 107 (36%) had a higher GS. After controlling for age and PSA, BMI was not associated with GS (odds ratio, OR=1.039, 95% confidence interval, CI=0.943-1.145; P=0.437). BMI had different effects on GS depending on VAT. When the data were stratified by the median VAT value, a higher BMI was significantly associated with a higher GS in patients with VAT⩾130.5 cm2 (OR=1.218, 95% CI=1.028-1.443; P=0.022), but not in those with VAT<130.5 cm2 (OR=0.912, 95% CI=0.783-1.062; P=0.236). A higher BMI was associated with an increased risk of high-grade cancer only in patients with more VAT.
Subject(s)
Neoplasm Recurrence, Local/pathology , Obesity, Abdominal/complications , Prostatectomy , Prostatic Neoplasms/pathology , Prostatic Neoplasms/surgery , Tomography, X-Ray Computed , Aged , Biomarkers, Tumor/blood , Body Mass Index , Humans , Male , Middle Aged , Neoplasm Recurrence, Local/diagnostic imaging , Obesity, Abdominal/diagnostic imaging , Obesity, Abdominal/pathology , Proportional Hazards Models , Prostate-Specific Antigen/blood , Prostatectomy/adverse effects , Prostatic Neoplasms/blood , Risk FactorsABSTRACT
STUDY DESIGN: Single case report. OBJECTIVES: To present a case of lumbar disc herniation causing compression of a tethered cord that was successfully treated with lumbar decompression and fusion. BACKGROUND: A tethered cord is a rare pathology associated with a congenital spinal malformation, spinal dysraphism. Furthermore, myelopathy due to lumbar disc herniation in the presence of a tethered cord is extremely rare. METHODS: Single case report. RESULTS: A 43-year-old male with a history of spina bifida presented to our clinic for an evaluation of a progressive spastic gait disturbance and numbness in the lower limbs. A neurological examination revealed muscle weakness and pyramidal tract signs in the lower limbs. Magnetic resonance imaging of the lumbar spine showed disc herniation at L2-3 causing compression of a low-lying cord. Surgical intervention, including herniotomy via a posterolateral approach and instrumented posterolateral fusion, was performed, and a good outcome was achieved 1 year after the surgery. CONCLUSION: The potential for lumbar disc herniation in the presence of a tethered cord should be taken into account in the differential diagnosis of spinal pathologies causing spastic gait disturbances. Furthermore, posterior decompression and fusion is a useful treatment option in such cases.
Subject(s)
Intervertebral Disc Displacement/complications , Neural Tube Defects/complications , Spinal Cord Diseases/etiology , Spinal Cord/pathology , Adult , Humans , Magnetic Resonance Imaging , Male , X-RaysABSTRACT
BACKGROUND AND PURPOSE: GLA is the causative gene of Fabry disease, an X-linked lysosomal storage disorder resulting from α-galactosidase A (α-GAL) deficiency. Stroke is an important manifestation of Fabry disease, and recent epidemiological studies have indicated that up to 4.9% of young male cryptogenic stroke patients have GLA mutations. To determine the importance of GLA mutations in the general stroke population, the frequency of GLA mutations in Japanese male ischaemic stroke (IS) patients with various risk factors and ages was measured. METHODS: A total of 475 male IS patients (mean age 69.7 ± 12.5 years), were enrolled in this study. A blood sample was obtained to produce blood spots for measurement of α-GAL activity. Blood samples with decreased enzymatic activity were reassayed and the entire GLA gene was analyzed by direct DNA sequencing if α-Gal A activity was consistently low. RESULTS: α-Gal A activity was decreased in 10 men, five of whom (1.1%) had the GLA gene mutation, p.E66Q. All IS patients with p.E66Q mutation had substantial residual α-Gal A activity, in contrast to patients with classic-type Fabry disease. Clinically, all patients with p.E66Q mutation were > 50 years old and had multiple small-vessel occlusions (lacunar infarctions). Statistical analysis using Fisher's exact test showed the allele frequency of GLA p.E66Q in patients with small-vessel occlusion to be significantly higher than that in the general Japanese population [odds ratio (OR) = 3.34, P = 0.025). CONCLUSIONS: GLA p.E66Q mutation is a genetic risk factor for cerebral small-vessel occlusion in elderly Japanese males.
Subject(s)
Mutation , Stroke/genetics , alpha-Galactosidase/genetics , Adult , Aged , Aged, 80 and over , Asian People , DNA Mutational Analysis , Humans , Male , Middle Aged , Risk Factors , Young AdultABSTRACT
Our aim was to evaluate the relationship between men's body mass index (BMI) and infertility and to examine the effects of factors related to metabolic syndrome such as hypertension, dyslipidaemia, and impaired glucose tolerance. Our sample comprised 74 healthy Japanese workers at a company who were married between 2003 and 2005. The outcome variable was whether a baby was born during the study period (median follow-up period, 20 months; range, 8-42 months). Data for BMI and other factors were obtained from the results of an annual health checkup in the year of each employee's marriage. Forty-seven men (64%) did not father a baby. Having a baby was significantly associated with a low BMI (21.4 versus 23.2 kg m(-2); P = 0.006). A Cox proportional hazard regression model was performed to assess the association of BMI with fathering a baby. Adjusting for age, systolic and diastolic blood pressure, low density lipoprotein cholesterol, triglycerides, and haemoglobin A(1C), higher BMI was significantly associated with not fathering a baby (hazard ratio, 0.80; 95% confidence interval, 0.67-0.95; P = 0012). High BMI in men was independently associated with an increased risk of not siring a child.
Subject(s)
Body Mass Index , Infertility, Male/etiology , Metabolic Syndrome/complications , Adolescent , Adult , Asian People , Humans , Japan , Male , Metabolic Syndrome/diagnosis , Middle Aged , Pilot Projects , Regression AnalysisSubject(s)
Amino Acids/blood , Umbilical Arteries , Umbilical Veins , Fetal Blood/chemistry , Humans , Infant, NewbornABSTRACT
To assess the utility of auditory brainstem response (ABR) in diagnosing brainstem changes in patients with Leigh syndrome (LS), we performed a longitudinal study of five patients with LS using both ABR and neuroimaging techniques (CT and MRI). The brainstem components of the initial ABRs we performed on the patients were abnormal in all five patients. In four of the patients, these abnormal findings preceded any clinical signs of brainstem impairment. Improvements in clinical findings were reflected in improvements in ABR findings in three patients. In one of these three patients, improvements in clinical findings were also reflected in improvements in MRI findings. In the other two patients, MRI findings showed no improvements, despite the improvements in clinical findings. In two of our patients, ABR clearly revealed functional improvements in the brainstem which were not revealed by MRI. Therefore, we conclude that ABR is an essential diagnostic technique for patients with LS.
Subject(s)
Brain Diseases/etiology , Brain Diseases/physiopathology , Brain Stem/pathology , Brain Stem/physiopathology , Evoked Potentials, Auditory, Brain Stem/physiology , Leigh Disease/complications , Leigh Disease/physiopathology , Adolescent , Adult , Brain Diseases/diagnosis , Brain Stem/diagnostic imaging , Child , Child, Preschool , Female , Humans , Leigh Disease/diagnosis , Longitudinal Studies , Magnetic Resonance Imaging , Male , Reproducibility of Results , Tomography, X-Ray ComputedABSTRACT
Hereditary tyrosinaemia type I (HT 1) (McKusick 276700) is caused by a deficiency of fumarylacetoacetate hydrolase (FAH) activity, the last enzyme in the tyrosine catabolic pathway. Homozygous disruption of the gene encoding FAH in mice (Fah) causes neonatal lethality (i.e. lethal Albino deletion c14CoS mice), which limits the use of this animal as a model for HT I. We developed a new mouse model that carries two genetic defects, Fah and 4-hydroxyphenylpyruvate dioxygenase (Hpd). The double mutant Fah -/- Hpd -/- mice grew normally without evidence of liver and renal disease, showing a phenotype similar to Hpd -/- mice. Complete blockage of the tyrosine catabolic pathway at the, step of HPD prevents development of clinical phenotypes. Administration of homogentisate resulted in rapid apoptosis of hepatocytes and renal tubular epithelial cells, a central feature of visceral injury in patients with HT I. Simultaneously, renal tubular function was impaired, resulting in Fanconi syndrome. Apoptosis of hepatocyte and renal tubular cells is prevented by the caspase inhibitors YVAD or DEVD. However, these inhibitors do not prevent the release of cytochrome c or the development of renal tubular dysfunction. Apoptosis of hepatocytes and of renal tubular epithelial cells are characteristic features of this disease and the apoptotic signal in this disease seems to be initiated by fumarylacetoacetate.
Subject(s)
Apoptosis , Hepatocytes/pathology , Kidney Tubules/pathology , Tyrosinemias/pathology , 4-Hydroxyphenylpyruvate Dioxygenase/genetics , Animals , Cytochrome c Group/metabolism , Disease Models, Animal , Humans , Hydrolases/genetics , Mice , Mice, Knockout , Mitochondria, Liver/enzymology , PhenotypeABSTRACT
Propionic acidaemia (PCCD) or deficiency of propionyl-CoA carboxylase (PCC) is one of the most common organic acidaemias. Recent studies have suggested that this disease can cause somatic or cognitive deterioration even in patients without ketosis or metabolic acidosis, or in cases with unusually late onset. This suggests that for this disease a sensitive yet practical screening procedure is required to achieve early treatment. We conducted a pilot study of gas chromatographic-mass spectrometric screening of 12,000 newborns for PCCD using eluates from dried filter-paper urine collected at 4-7 days of age. Methylcitrate (MC) was targeted for PCCD. For bulk screening, 2-hydroxyundecanoate was used as internal standard; for quantification, stable-isotope-labelled MC was used. Urease pretreatment without fractionation allowed satisfactory recovery and reproducibility of the highly polar MC. We detected an asymptomatic male infant with distinctly elevated MC: the creatinine-corrected level relative to 2-hydroxyundecanoate was 4.8 SD above the normal mean. The MC concentration calculated using the stable-isotope-labelled internal standard was 70.6 mmol/mol creatinine 14.7 SD above the normal mean of 3.70. Parallel analysis of the dried blood spot at 4 days of age by tandem MS showed only borderline elevation of propionylcarnitine. The activity of PCC in lymphocytes was 7% of control. Gene analysis revealed that a single missense mutation, TAT to TGT, resulting in Y435C in the beta chain was present in a homozygous form. Dietary treatment including carnitine supplementation decreased this infant's MC level and to date (at 13 months of age), he shows no neurological or somatic abnormalities.
Subject(s)
Carboxy-Lyases/deficiency , Carnitine/analogs & derivatives , Citrates/urine , Gas Chromatography-Mass Spectrometry , Neonatal Screening , Paper , Propionates/blood , Carboxy-Lyases/genetics , Carnitine/blood , DNA Mutational Analysis , Homozygote , Humans , Infant, Newborn , Male , Mass Spectrometry , Methylmalonyl-CoA Decarboxylase , Mutation, MissenseSubject(s)
Oculocerebrorenal Syndrome , Phosphoric Monoester Hydrolases , DNA, Complementary/genetics , Diagnosis, Differential , Genetic Linkage , Humans , Mutation , Oculocerebrorenal Syndrome/genetics , Oculocerebrorenal Syndrome/physiopathology , Prognosis , Proteins/genetics , X Chromosome/geneticsABSTRACT
Congenital insensitivity to pain with anhidrosis is an autosomal recessive hereditary disorder characterized by recurrent episodic fever, anhidrosis (inability to sweat), absence of reaction to noxious stimuli, self-mutilating behavior, and mental retardation. The human TRKA gene (NTRK1), located on chromosome 1q21-q22 encodes the receptor tyrosine kinase for nerve growth factor. We reported that TRKA is the gene responsible for CIPA and we developed a comprehensive strategy to screen for TRKA mutations and polymorphisms, as based on the gene's structure and organization. Here we report eight novel mutations detected as either a homozygous or heterozygous state in nine CIPA families from five countries. Mendelian inheritance of the mutations was confirmed in seven families for which samples from either parent were available. However, non-mendelian inheritance seems likely for the family when only samples from the mother and siblings, (but not from the father) were available. A paternal uniparental disomy for chromosome 1 is likely to be the cause of reduction to homozygosity of the TRKA gene mutation in this family. Interestingly, a Hispanic patient from the USA has two autosomal genetic disorders, CIPA and pyruvate kinase deficiency, whose genetic loci are both mapped to a closely linked chromosomal region. A splice mutation and a missense mutation were detected in the TRKA and PKLR genes from the homozygous proband, respectively. Thus, concomitant occurrence of two disorders is ascribed to a combination of two separate mutant genes, not a contiguous gene syndrome. This finding suggests a mechanism responsible for two autosomal genetic disorders in one patient. All these data further support findings that TRKA defects can cause CIPA in various ethnic groups. This will aid in diagnosis and genetic counseling of this painless but severe genetic disorder.
Subject(s)
Genetic Linkage/genetics , Hereditary Sensory and Autonomic Neuropathies/genetics , Mutation/genetics , Pyruvate Kinase/deficiency , Pyruvate Kinase/genetics , Receptor, trkA/genetics , Uniparental Disomy/genetics , Adolescent , Adult , Alleles , Child , Child, Preschool , Chromosome Mapping , Chromosomes, Human, Pair 1/genetics , DNA Mutational Analysis , Female , Haplotypes/genetics , Hereditary Sensory and Autonomic Neuropathies/complications , Hereditary Sensory and Autonomic Neuropathies/enzymology , Humans , Infant , Infant, Newborn , Male , Pedigree , Polymorphism, Genetic/genetics , Pyruvate Kinase/metabolismSubject(s)
Amino Acid Metabolism, Inborn Errors , Proline/blood , Female , Humans , Male , Proline Oxidase/deficiencyABSTRACT
Chronic granulomatous disease (CGD) is an inherited disorder of host defense against microbial infections caused by defective activity of the phagocyte NADPH oxidase. Based on an increase of neutrophil superoxide-generating ability in response to interferon gamma (IFN-gamma) in a single patient with CGD, multicentered group studies demonstrated a beneficial effect of prophylactic IFN-gamma. However, no apparent increase of the phagocyte superoxide generation was found in patients enrolled in these studies. The present report offers an additional kindred in whom an IFN-gamma-dependent increase in neutrophil superoxide production was observed in 3 affected patients. The defect in the CYBB gene for gp91-phox was identified as an otherwise silent mutation adjacent to the third intron of the CYBB gene that alters messenger RNA splicing. By molecular analysis, significant differences were found in the splicing pattern of CYBB gene transcripts in patient neutrophils between 1 and 25 days after administration of IFN-gamma. Furthermore, a complete transcript containing the missing exons could be detected in all specimens after the treatment. The changes in the splicing pattern of the transcripts and the prolonged effect on superoxide-generating ability of patient neutrophils indicate that IFN-gamma induced a partial correction of the abnormal splicing of CYBB gene transcripts in myeloid progenitor cells.
Subject(s)
Interferon-gamma/pharmacology , Membrane Glycoproteins/genetics , NADPH Oxidases , Neutrophils/chemistry , RNA Splicing , RNA, Messenger/genetics , Superoxides/blood , Adolescent , Female , Flow Cytometry , Granulomatous Disease, Chronic/genetics , Humans , Male , Mutation , NADPH Oxidase 2 , PedigreeABSTRACT
Human TRKA (NTRK1) encodes the receptor tyrosine kinases (RTKs) for nerve growth factor (NGF) and is the gene responsible for congenital insensitivity to pain with anhidrosis (CIPA), an autosomal recessive disorder characterized by a lack of pain sensation and anhidrosis. We reported 11 putative missense mutations in 31 CIPA families from various ethnic groups. Here we have introduced the corresponding mutations into the TRKA cDNA and examined NGF-stimulated autophosphorylation. We find that wild-type TRKA precursor proteins in a neuronal and a non-neuronal cell line were differentially processed and phosphorylated in an NGF-dependent and -independent manner, respectively. Two mutants (L93P and L213P) in the extracellular domain were aberrantly processed and showed diminished autophosphorylation in neuronal cells. Five mutants (G516R, G571R, R643W, R648C and G708S) in the tyrosine kinase domain were processed as wild-type TRKA but showed significantly diminished autophosphorylation in both neuronal and non-neuronal cells. In contrast, R85S and (H598Y; G607V), detected previously as double and triple mutations, are probably polymorphisms in a particular ethnic background. The other putative mutant D668Y might be a rare polymorphism or might impair the function of TRKA without compromising autophosphorylation. Mutated residues in the tyrosine kinase domain are conserved in various RTKs and probably contribute to critical function of these proteins. Thus, naturally occurring TRKA missense mutations with loss of function provide considerable insight into the structure-function relationship in the RTK family. Our data may aid in developing a drug which targets the clinically devastating 'complex regional pain syndrome'.
Subject(s)
Hypohidrosis/genetics , Pain Insensitivity, Congenital/genetics , Receptor, trkA/genetics , Amino Acid Sequence , Animals , Binding Sites/genetics , COS Cells , DNA, Complementary/genetics , DNA, Recombinant , Genotype , Humans , Hypohidrosis/pathology , Immunoblotting , Molecular Sequence Data , Mutation, Missense , Nerve Growth Factor/metabolism , Pain Insensitivity, Congenital/pathology , Phosphorylation , Plasmids/genetics , Protein Structure, Tertiary , Receptor, trkA/chemistry , Receptor, trkA/metabolism , Sequence Alignment , Sequence Homology, Amino Acid , Transfection , Tumor Cells, CulturedABSTRACT
Carbamoyl phosphate synthetase I deficiency (CPSID) is a rare metabolic disorder affecting the first enzymatic step of urea cycle. We report clinical manifestations of a female case of late-onset CPSID in Japan. An 18-year-old girl was admitted to emergency room due to acute comatose state. Her parents had no apparent consanguineous history. She had suffered from intermittent psychotic episodes (excitation, aggressive behavior and insomnia) with nausea and vomiting from the age of 13, mostly coinciding with menstrual period. She had minor learning disability without major neurological deficits and convulsions. Her mental status was estimated as normal in her intermenstrual period. She had been diagnosed as having hysteria and premenstrual syndrome. Her neurological findings on admission showed deep coma and hypotonic tetraparesis. Plasma ammonia level was markedly elevated (684 micrograms/dl) without significant liver dysfunction. Blood urea nitrogen decreased to 6 mg/dl. Continuous venovenous filtration with subsequential administration of sodium benzoate and l-arginine was started to eliminate blood ammonia. Although the plasma ammonia level decreased to 300 mu/dl in next 10 hours, severe cerebral edema was observed in head computed tomography subsequently, followed by marked cerebral atrophy. Finally, her consciousness status became almost alert a month after the onset, but her mental status was severely retarded. CPSI activity of her biopsied liver markedly decreased and she was diagnosed as having CPS ID. CPSI cDNA analysis of her biopsied liver demonstrated a V1149G mutation. Genomic DNA analysis showed that she was heterozygous in V1149G mutation. The mutation allele was derived from her father. The causative factor for absence or very low level of maternal CPSI mRNA will require further analysis.