Development of chitosan-graphene oxide blend nanoparticles for controlled flurbiprofen delivery.

The use of natural polymeric nanoparticles (Nps) as drug carriers is a highly promising area of research in the field of drug delivery systems because of their high efficiency. In this study, flurbiprofen (FB) loaded chitosan-graphene oxide (CS-GO) blend Nps were synthesized as a controlled delivery system using the emulsion method. The crystalline, molecular, and morphological structures of the prepared CS-GO Nps were characterized using a variety of analytical methods, including Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-Ray diffractometry (XRD), scanning electron microscopy (SEM), and atomic force microscopy (AFM). It was found that the introduction of GO into the CS nanoparticle formulation increased its thermal stability. The range of the average particle size was between 362 ± 5.06 and 718 ± 2.21 nm, with negative zeta potential values between -7.67 ± 4.16 and - 27.93 ± 2.26 mV. The effects of the CS/GO ratio, the FB/polymer ratio, the amount of span 80, and the cross-linker concentration were assessed on FB release profiles. In vitro release studies displayed a two-stage release behaviour with a fast initial release of the FB, followed by sustained and extended release, and the incorporation of GO into the CS Nps made the FB release more sustained and controlled manner. Besides, the cytotoxicity test of the FB-loaded CS-GO Nps was studied through MTT assay, and it was found that they were biocompatible. Based on these findings, it can be inferred that the prepared CS-GO Nps might be a promising candidate drug carrier system for FB.

Design and evaluation of temperature-responsive chitosan/hydroxypropyl cellulose blend nanospheres for sustainable flurbiprofen release.

In present work, temperature-responsive flurbiprofen (FLU) containing chitosan/hydroxypropyl cellulose (CS/HPC) blend nanospheres were prepared using emulsion method. The structures of blend nanospheres were characterized by ATR-FTIR, XRD, SEM, DSC/TGA, zeta potential and particle size analyses. Their lower critical solution temperatures (LCST) were determined and found to be 42 °C. In vitro release studies were performed in gastrointestinal-tract simulated conditions at 30 °C, 37 °C and 44 °C. As the medium temperature was increased, the release of FLU decreased, indicating that blend nanospheres had temperature-responsive feature. The FLU release demonstrated that release profiles depend upon CS/HPC ratio, amount of FLU present in the nanospheres and percentage of cross-linker used. Moreover, the cytotoxicity tests were performed via MTT method and it was observed CS/HPC nanospheres were biocompatible. Based on the in vitro release profile and cytotoxicity studies, the fabricated CS/HPC blend nanospheres could be a promising candidate as a temperature-responsive nano-carrier for controlled drug release.