Native Mexican black bean purified anthocyanins fractionated by high-performance counter-current chromatography modulate inflammatory pathways.

In addition to their pigment properties, the potential health benefits of anthocyanins have made them a subject of interest in recent years. This study aimed to obtain purified anthocyanin fractions from native Mexican black bean cultivars using Amberlite XAD-7 resin column and HPCCC and evaluate their anti-inflammatory properties using RAW 264.7 cells. The major anthocyanins in the purified anthocyanin fractions were delphinidin 3-glucoside (61.8%), petunidin 3-glucoside (25.2%), and malvidin 3-glucoside (12.2%). Purified anthocyanin fractions at 12.5 µg/mL effectively prevented LPS-induced ERK1/ERK2 phosphorylation and reduced the protein expression of COX-2 and mRNA expression of iNOS. Results showed that purified anthocyanin fractions have the potential to modulate the inflammatory response by inhibiting the production of pro-inflammatory mediators through the ERK1/ERK2 and NF-κB pathways. This study suggests that anthocyanins from black beans could be used as a natural strategy to help modulate inflammation-associated diseases.

Common beans as a source of food ingredients: Techno-functional and biological potential.

Common beans are an inexpensive source of high-quality food ingredients. They are rich in proteins, slowly digestible starch, fiber, phenolic compounds, and other bioactive molecules that could be separated and processed to obtain value-added ingredients with techno-functional and biological potential. The use of common beans in the food industry is a promising alternative to add nutritional and functional ingredients with a low impact on overall consumer acceptance. Researchers are evaluating traditional and novel technologies to develop functionally enhanced common bean ingredients, such as flours, proteins, starch powders, and phenolic extracts that could be introduced as functional ingredient alternatives in the food industry. This review compiles recent information on processing, techno-functional properties, food applications, and the biological potential of common bean ingredients. The evidence shows that incorporating an adequate proportion of common bean ingredients into regular foods such as pasta, bread, or nutritional bars improves their fiber, protein, phenolic compounds, and glycemic index profile without considerably affecting their organoleptic properties. Additionally, common bean consumption has shown health benefits in the gut microbiome, weight control, and the reduction of the risk of developing noncommunicable diseases. However, food matrix interaction studies and comprehensive clinical trials are needed to develop common bean ingredient applications and validate the health benefits over time.

Low glycemic index common bean snack increased satiety without modifying energy intake in adults with normal weight: randomized crossover trials.

This study aimed to evaluate the short-term effect of a common bean baked snack (CBBS) and cooked bean consumption on energy intake, satiety, glycemic response, and palatability in subjects with normal weight (Study 1) and overweight (Study 2) and to determine the glycemic index of CBBS (Study 3). For studies 1 and 2, satiety and glycemic response were measured over 45 min after consuming CBBS, cooked beans or white bread preload, and energy intake at an ad libitum test meal was calculated. Energy intake remained similar after consuming the three preloads in both studies. Compared to white bread, CBBS consumption increased fullness by 52% in subjects with normal weight but not in those with overweight. The CBBS calculated glycemic index was considered low (42). Consumption of low glycemic index CBBS increased satiety in adults with a normal weight. Long-term trials assessing the effects on body weight management are necessary.

Common Bean Baked Snack Consumption Reduces Apolipoprotein B-100 Levels: A Randomized Crossover Trial.

Snack alternatives based on common beans (Phaseolus vulgaris L.) have been developed to promote pulse consumption. The purpose of this study was to evaluate the chemical composition, sensory acceptance and the effect of common bean baked snack (CBBS) consumption on blood lipid levels in participants with overweight and altered blood lipid levels. A sensory evaluation by 80 untrained judges was carried out using a hedonic scale. A randomized crossover 2 × 2 trial was performed, where 20 participants with overweight and one blood lipid alteration consumed 32 g of CBBS or did not consume it (control) for four weeks. Blood samples were taken to quantify the triglycerides, total cholesterol, LDL-c, HDL-c, ApoB-100, glucose and insulin. Furthermore, anthropometric, dietary and physical activity parameters were recorded. The overall acceptance of CBBS was similar compared to popcorn (p > 0.05). The consumption of CBBS reduced the apolipoprotein B-100 levels (p = 0.008). This reduction could be associated with the additional dietary fiber consumption during the CBBS period (p = 0.04). Although it did not improve any other blood lipid or glucose parameters (p > 0.05), it did not affect them either, which means that the CBBS could be consumed without compromising cardiovascular health.

Pulse-based snacks as functional foods: Processing challenges and biological potential.

Despite their high nutritional value and potential health benefits, pulse intake has not increased in the last three decades. Several strategies have been implemented to increase pulse consumption, such as their incorporation in bakery products, breakfast cereals, and snacks. The inclusion of pulses in these products could be an alternative to satisfy the consumers' demand for healthy foods. However, pulse-based snacks face important challenges, including reducing antinutritional factors, achieving consumer acceptance, and consolidating the pulse-based snacks as functional foods. This review summarizes and discusses methods for producing snacks where cereals or tubers were replaced with at least 50% pulses. Also, it briefly assesses their effect on nutritional composition, antinutritional factors, sensory acceptance, and different health benefits evaluations. Extruded snacks exhibited high protein and dietary fiber and low fat content, contrary to the high fat content of deep fat-fried snacks. Meanwhile, baked snacks presented moderate concentrations of protein, dietary fiber, and lipids. Pulses must be pretreated using process combinations such as soaking, dehulling, cooking, fermentation, germination, and extrusion to reduce the antinutritional factors. Pulse-based snacks show good sensory acceptance. However, sensory evaluation should be more rigorous using additional untrained judges. Several studies have evaluated the health benefits of pulse-based snacks. More research is needed to validate scientifically the health benefits associated with their consumption. Pulse-based snacks could be an alternative to improve the nutritional composition of commercially available snacks. The use of pulses as ingredients of healthier snacks represents an important alternative for the food industry due to their low cost, sensory characteristics, high nutritional profile, and environmental benefits.

Autoclaving and extrusion improve the functional properties and chemical composition of black bean carbohydrate extracts.

Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments. The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans. After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis. Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE. Meanwhile, extrusion showed a reduction in resistant starch of 54.2%. Autoclaving and extrusion treatments also decreased the dietary fiber content. Extrusion reduced almost entirely the content of α-galactooligosaccharides, in comparison to dehulled raw bean CE. The results showed differences in color and granule morphology. The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE. The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products. PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein. Because these extracts are already cooked, they can be added to products that do not require a thermal process such as puddings, sauces, creams, or dairy products, acting as a thickening agent.

Percepción de riesgo sobre lesiones no intencionales en niños y niñas, México / The perception of risk of unintentional injuries among children in Mexico / Percepção de risco sobre lesões não intencionais em meninos e meninas no México

Resumen (analítico) Es posible evitar las lesiones no intencionales, particularmente en los menores, esto se ve obstruido por la percepción de los cuidadores. La narrativa recupera la experiencia de los cuidadores, padres, familiares, analizando la visión sobre las lesiones, en hijos o menores a cargo, hospitalizados. A partir de los resultados se percibe que hay una reducción de la percepción de riesgo en los cuidadores y carencia en la información de medidas preventivas. Además, una creencia de carencia de control, cedido al destino. Se concluye que existe una ingenuidad preventiva en la sobreestimación de las capacidades de los niños. Una percepción de riesgo, creencias y conductas que reducen la valoración del peligro. Roles de género y estatus socio-económico son bases de esta visión fatalista. Resultando la reducción de capacidades de prevención, hacia hijos o menores a cargo.

Abstract (analytical) It is possible to avoid unintentional injuries, particularly in children. This is affected by the perceptions of caregivers. This study collects the experiences of caregivers, parents and relatives, analyzing their visions of injuries of their children or children under their care who have been hospitalized. Based on the results it is perceived that there is a reduction of the perception of risk among caregivers and a lack of information on preventive measures. In addition, there is a belief that they have a lack of control and that it is unavoidable that children injure themselves. The authors conclude that there is a preventative naivety that exists among parents and caregivers and an overestimation of the abilities of children. There are perceptions of risk, beliefs and behaviors that reduce the levels of danger perceived by adults. Traditional gender roles and socio-economic status form the basis of this fatalistic vision. This results in a reduction of prevention capacities that protect adults' children and children under their care.

Resumo (analítico) É possível evitar lesões não intencionais, particularmente em crianças, ainda que obstruídas pela percepção dos cuidadores. A narrativa recupera a experiência dos pais ou familiares cuidadores, analisando a visão sobre as lesões, em crianças ou menores responsáveis, internados. A partir dos resultados nota-se que há redução na percepção do risco nos cuidadores e falta de informação sobre medidas cautelares. Além disso, uma crença de falta de controle, dada ao destino. Há também ingenuidade preventiva e superestimação das capacidades das crianças. Uma percepção de risco, crenças e comportamentos que reduzem a avaliação do perigo. Os papéis de gênero e o status socioeconômico são a base dessa visão fatalista. Redução resultante de capacidades de prevenção, para crianças ou menores responsáveis.

[Wrist arthroscopy for fractures of the distal end of the radius]. / Artroscopía de muñieca en fracturas del extremo distal del radio.

INTRODUCTION: Fractures of the distal end of the radius are very frequent in the orthopedist's practice. The anatomical reduction of the articular surface and the extra-articular alignment of the radius are extremely important to minimize complications. The purpose of the study is to make an arthroscopic diagnosis of the lesions associated to the distal radius and assist in the reduction of the distal radius fracture. MATERIAL AND METHODS: The study was conducted from March 2007 to February 2008. We performed 20 wrist arthroscopies in 20 patients with an intraarticular fracture of the distal end of the radius. They were classified according to Frykman's criteria. Patient age ranged from 17 to 67, with a mean of 38.7 +/- 15.9 years. 60% were males and 40% females; mean follow-up was 12 months; the chondral lesions were classified according to Outerbridge, the interosseous ligament lesions and the triangular fibrocartilage lesions, according to Palmer's criteria. We did articular debridement, radiofrequency chondroplasty, and assisted the fracture reduction. We used Henry's palmar approach and did open reduction and internal fixation with a T-shaped palmar plate and finally performed an arthroscopy to check the reduction. RESULTS: 8 cases had chondral lesions (grades II to IV); 7 cases had lesion of the triangular fibrocartilage (central isolated ruptures, radial detachment of the triangular fibrocartilage from the sigmoid notch), 5 cases had lesion of the scapholunate ligament; one case had a lesion of the luno-pyramidal ligament, and in 6 cases no associated lesions were found; 8 cases had a single lesion and 6 cases had 2 to 3 concomitant lesions. Two patients had been lost at the 3- and 6-month follow-up visits; 35% of those that were followed-up had pain at 3 months (7 patients) and 15% at 6 months. Only 2 cases had scapholunate instability and only one case had distal radioulnar instability. CONCLUSIONS: Arthroscopy has proven useful in identifying hidden lesions, assisting in fracture reduction and even treating the lesions found at the time of the diagnosis. Chondral lesions were the most frequent ones, followed by lesions of the triangular fibrocartilage; a close relation was found between the type of Frykman fracture and the associated lesion. Type 7 and 8 lesions were more frequently associated with chondral lesions and with a higher mean age. At the end of the follow-up period the scapholunate ligament lesions remained unstable. Chondral lesions were related with persistent pain at 3 months.

[Vertebral destruction syndrome. Diagnostic evaluation systems]. / Síndrome de destrucción vertebral. Sistemas de evaluación en su diagnóstico.

BACKGROUND: Vertebral Destruction Syndrome (VDS) is a pathology of multiple etiologies causing structural alterations of the spine, producing deformity with neurological and mechanical alterations. In order to study VDS, a diagnostic process is carried out, sometimes with unexpected results. We undertook this study to validate the systematization of a series of studies to arrive at the diagnosis of VDS. METHODS: We included 105 patients in the study with diagnosis of VDS from January 1998 to December 2005, taking into consideration specificity, sensitivity and predictive value of each integrated study in order to determine its diagnostic value. RESULTS: The most frequent etiology was Pott's Disease (24 cases) followed by osteomyelitis (20 cases), metastasis (18 cases) and multiple myeloma and plasmacytoma (16 cases each). The higher sensitivity in Pott's Disease was obtained with bone scan and polymerase chain reaction (PCR); for multiple myeloma, computerized axial tomography (CAT) and bone scan; CAT and bone scan for infections; MRI for primary tumors; and MRI and bone scan for secondary tumors. CONCLUSIONS: To reduce false positives to 2% and to reduce the maximum number of false negatives, studies such as CAT, MRI, bone scan, PCR, ESR, C-reactive protein and determination of alkaline and acid phosphatase must be included in the VDS study protocol. Other studies have very low diagnostic sensitivity and specificity.

Síndrome de destrucción vertebral: sistemas de evaluación en su diagnóstico / Vertebral destruction syndrome: diagnostic evaluation systems

BACKGROUND: Vertebral Destruction Syndrome (VDS) is a pathology of multiple etiologies causing structural alterations of the spine, producing deformity with neurological and mechanical alterations. In order to study VDS, a diagnostic process is carried out, sometimes with unexpected results. We undertook this study to validate the systematization of a series of studies to arrive at the diagnosis of VDS. METHODS: We included 105 patients in the study with diagnosis of VDS from January 1998 to December 2005, taking into consideration specificity, sensitivity and predictive value of each integrated study in order to determine its diagnostic value. RESULTS: The most frequent etiology was Pott's Disease (24 cases) followed by osteomyelitis (20 cases), metastasis (18 cases) and multiple myeloma and plasmacytoma (16 cases each). The higher sensitivity in Pott's Disease was obtained with bone scan and polymerase chain reaction (PCR); for multiple myeloma, computerized axial tomography (CAT) and bone scan; CAT and bone scan for infections; MRI for primary tumors; and MRI and bone scan for secondary tumors. CONCLUSIONS: To reduce false positives to 2% and to reduce the maximum number of false negatives, studies such as CAT, MRI, bone scan, PCR, ESR, C-reactive protein and determination of alkaline and acid phosphatase must be included in the VDS study protocol. Other studies have very low diagnostic sensitivity and specificity.

Perforación intestinal por anisakiasis / Ileal perforation due to anisakiasis

La anisakiasis es una parasitosis con un número creciente de casos en nuestro entorno en los últimos años. Manifestada como abdomen agudo, simula otras etiologías y es habitualmente de indicación quirúrgica. Se expone un caso cuya excepcional forma de presentación fue la perforación ileal (AU)