ABSTRACT
Peanuts (Arachis hypogaea L.) are among the most important leguminous crops in Argentina. During the growing season, they are frequently attacked by fungal diseases, including Thecaphora frezii. The spores of T. frezii are structures that confer resistance to this phytopathogen. The transition from teliospore to hypha is a characteristic process of some fungi, which is essential for completing their life cycle. Using the transcriptomes of teliospores and hyphae of T. frezii, we aimed to identify genes that were differentially expressed during this transition, and we found 134 up-regulated and 66 down-regulated genes, which would participate in different cellular processes such as: (a) cell cycle and DNA processing; (b) cell fate; (c) rescue, defense and cellular virulence; (d) detoxification by CYP450; (e) energy; (f) nutrient interaction and nutritional adaptation; (g) metabolism; (g) proteins with binding functions or cofactor requirements; (h) stress, cell differentiation and biogenesis of cell components; and (i) transport, cell communication and transcription. The identification of genes in T. frezii and their expression levels during different stages of differentiation could contribute to our understanding of the biological mechanisms in this fungus.
Subject(s)
Arachis , Hyphae , Spores, Fungal , Arachis/microbiology , Hyphae/genetics , Hyphae/growth & development , Spores, Fungal/genetics , Plant Diseases/microbiology , Gene Expression Regulation, Fungal , Genes, Fungal , Fungal Proteins/genetics , Transcriptome , Gene Expression ProfilingABSTRACT
We present the draft genome sequence of Gordonia sp. strain Campus, which was extracted from diesel-contaminated soil in Córdoba, Argentina. It was observed that this strain, in conjunction with alfalfa and poplar, has the ability to decompose diesel-contaminated soils. The data may be important for the phytoremediation of hydrocarbon-contaminated soils.
ABSTRACT
Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. Other members of the family replicate in viral inclusion bodies (VIBs) termed viroplasms that are formed in the cytoplasm of infected plant and insect cells. In this study, the protein coded by the first ORF of MRCV segment S9 (P9-1) was shown to establish cytoplasmic inclusion bodies resembling viroplasms after transfection of Spodoptera frugiperda insect cells. In accordance, MRCV P9-1 self-associates giving rise to high molecular weight complexes when expressed in bacteria. Strong self-interaction was also evidenced by yeast two-hybrid assays. Furthermore, biochemical characterization showed that MRCV P9-1 bound single stranded RNA and had ATPase activity. Finally, the MRCV P9-1 region required for the formation of VIB-like structures was mapped to the protein carboxy-terminal half. This extensive functional and biochemical characterization of MRCV P9-1 revealed further similarities between plant and animal reovirus viroplasm proteins.
Subject(s)
Inclusion Bodies, Viral/metabolism , Reoviridae/metabolism , Spodoptera/virology , Viral Proteins/metabolism , Amino Acid Motifs , Animals , Inclusion Bodies, Viral/chemistry , Inclusion Bodies, Viral/genetics , Open Reading Frames , Reoviridae/chemistry , Reoviridae/genetics , Viral Proteins/chemistry , Viral Proteins/geneticsABSTRACT
Apotransferrin (aTf), has been shown to accelerate the differentiation of oligodendroglial cells (OLGcs) in primary cultures and to increase the expression of different components of the myelin cytoskeleton (CSK). We examined the incorporation and distribution of human aTf (aTfh) exogenously added to OLGcs cultures and its effects on the CSK of the OLGcs. When OLGcs treated with aTfh were extracted with a CSK-stabilizing buffer containing detergent, aTfh was found in the soluble fraction. In vitro experiments showed that purified tubulin was not altered by the addition of aTfh. In OLGc primary cultures treated with aTfh, this glycoprotein showed a punctate distribution pattern along the OLGc processes. Treatment of the cultures with colchicine, cytochalasin, or taxol induced a displacement of the immunoreactivity of aTfh toward the OLGc soma. Analysis of the effects of aTfh on the cell distribution of tyrosinated and detyrosinated tubulin and STOP (stable tubule only polypeptide), showed that aTfh added to OLGc cultures promoted changes suggesting a stabilizing effect on the microtubules (MT) at the tip of the processes. Kinesin and dynein were found to colocalize with the aTfh, indicating that these motors participate in the transport of the added glycoprotein. Moreover, after treatment with aTfh, clathrin immunoreactivity was displaced from the OLGc body toward the cell processes. These results indicate that although aTfh added to OLGcs does not interact directly with CSK components, it seems to be transported in clathrin coated vesicles from the cell body to the tips of the OLGc processes where it promotes their stabilization. This mechanism may be of importance in the increased formation of the myelin membrane induced by aTf.
Subject(s)
Apoproteins/pharmacology , Cytoskeleton/drug effects , Gene Expression Regulation/drug effects , Oligodendroglia/drug effects , Transferrin/pharmacology , Animals , Animals, Newborn , Blotting, Western/methods , Cells, Cultured , Cerebral Cortex/cytology , Cytochalasins/pharmacology , Cytoskeleton/metabolism , Gene Expression Regulation/physiology , Humans , Immunohistochemistry/methods , Microscopy, Confocal/methods , Microtubule-Associated Proteins/metabolism , Oligodendroglia/metabolism , Rats , Rats, Wistar , Receptors, Transferrin/metabolism , Tubulin/metabolismABSTRACT
El objetivo de este trabajo es presentar la experiencia inicial en safenectomía y ligadura de perforantes (Linton) ambulatorias. El programa implementado consiste en el ingreso al hospital a las 6,00, anestesia peridural a las 6,30, cirugía a las 7,00, fin de cirugía 8,45, con internación transitoria con vendaje elástico ajustado. Luego de control, alta alrededor de las 18,00. Se operaron, en un período de 6 meses, 10 mujeres y 5 hombres en su gran mayoría con dolor, pesadez, edema y prurito. Se realizó safenectomía y ligadura de perforantes según técnica clásica. Duración del período de internación: 12 hs. Complicaciones: 1 hematoma de la ingle. Número de consultas externas desde el diagnóstico hasta el alta en la 5* semana: 4. Todos los pacientes se reintegraron a sus tareas habituales en el 8* días postoperatorio. La cirugía venosa mayor puede realizarse en un programa ambulatorio con buena tolerancia por parte del paciente. El procedimiento resultó ser eficiente, sin complicaciones y expeditivo. Se continuará con el programa para informar sobre su resultado alejado en un número mayor de pacientes
Subject(s)
Varicose Veins/surgery , Ambulatory Surgical Procedures/methods , Ambulatory Surgical Procedures/statistics & numerical data , Saphenous Vein/surgery , Extremities , Leg , ThighABSTRACT
El objetivo de este trabajo es presentar la experiencia inicial en safenectomía y ligadura de perforantes (Linton) ambulatorias. El programa implementado consiste en el ingreso al hospital a las 6,00, anestesia peridural a las 6,30, cirugía a las 7,00, fin de cirugía 8,45, con internación transitoria con vendaje elástico ajustado. Luego de control, alta alrededor de las 18,00. Se operaron, en un período de 6 meses, 10 mujeres y 5 hombres en su gran mayoría con dolor, pesadez, edema y prurito. Se realizó safenectomía y ligadura de perforantes según técnica clásica. Duración del período de internación: 12 hs. Complicaciones: 1 hematoma de la ingle. Número de consultas externas desde el diagnóstico hasta el alta en la 5* semana: 4. Todos los pacientes se reintegraron a sus tareas habituales en el 8* días postoperatorio. La cirugía venosa mayor puede realizarse en un programa ambulatorio con buena tolerancia por parte del paciente. El procedimiento resultó ser eficiente, sin complicaciones y expeditivo. Se continuará con el programa para informar sobre su resultado alejado en un número mayor de pacientes