ABSTRACT
The aim of this study was to evaluate flower and leaf methanol extracts of Artemisia alba Turra for their total phenolic and flavonoid contents, antioxidant capacity and to investigate their phenolic composition. The flower extract was richer in total phenolics and flavonoids and possessed higher antioxidant activity through DPPH and ABTS assays. The UHPLC-PDA-MS analysis of the flower and leaf methanol extracts revealed similar phenolic profile and allowed identification of 31 phenolic compounds (flavonoids, coumarins, and phenolic acids) by comparison with the respective reference compounds or tentatively characterized by their chromatographic behavior, UV patterns, and MS fragmentations. The presence of hispidulin, jaceosidin, desmethoxycentaureidin, and dicaffeoyl esters of quinic acid in A. alba is reported herein for the first time. The distribution of flavonoids in A. alba from different origins was discussed from chemotaxonomic point of view.
Subject(s)
Antioxidants/pharmacology , Artemisia/chemistry , Flowers/chemistry , Phenols/pharmacology , Plant Leaves/chemistry , Antioxidants/chemistry , Antioxidants/isolation & purification , Benzothiazoles/antagonists & inhibitors , Biphenyl Compounds/antagonists & inhibitors , Flavonoids/chemistry , Flavonoids/isolation & purification , Flavonoids/pharmacology , Molecular Structure , Phenols/chemistry , Phenols/isolation & purification , Picrates/antagonists & inhibitors , Sulfonic Acids/antagonists & inhibitorsABSTRACT
Ten new sesquiterpene alcohols of which seven germacranes, a eudesmane, a guaiane and an oplopane were isolated from the aerial parts of Artemisia alba Turra. Their structures and relative stereochemistry were elucidated by spectral methods ((1)H and (13)C NMR, COSY, HSQC, HMBC, NOESY, and MS). In addition, the known 7-hydroxycadin-4-en-3-one, centaureidin and axillarin were found for the first time in the studied species.
Subject(s)
Artemisia/chemistry , Oxygen/chemistry , Sesquiterpenes, Eudesmane/isolation & purification , Sesquiterpenes, Guaiane/isolation & purification , Sesquiterpenes/isolation & purification , Alcohols/chemistry , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Sesquiterpenes/chemistry , Sesquiterpenes, Eudesmane/chemistry , Sesquiterpenes, Guaiane/chemistryABSTRACT
Alkaloid pattern of individuals from 16 Bulgarian Galanthus elwesii populations was investigated by GC/MS and TLC. Twenty-one Amaryllidaceae alkaloids were detected and 14 of them were identified. Crinane type alkaloids, haemanthamine or crinine, dominated alkaloid metabolism in most of the populations. With exception of one population, where the separate individuals showed variable alkaloid profiles (dominated by crinine or haemanthamine) the individuals of the rest of populations have identical and characteristic alkaloid profiles. Some populations showed remarkable differences in respect to their alkaloid pattern-type of biosynthesis, main alkaloids and number of alkaloids. Populations dominated by galanthamine type alkaloids were found as well. These data demonstrate that like the morphological features, the alkaloid metabolism of G. elwesii is also variable.
Subject(s)
Alkaloids/metabolism , Galanthus/metabolism , Alkaloids/chemistry , Chromatography, Thin Layer , Galanthus/chemistry , Galanthus/classification , Gas Chromatography-Mass Spectrometry , Species SpecificityABSTRACT
A GC/MS analysis of alkaloids from leaves, bulbs and roots of Pancratium maritimum was performed. From the identified 16 alkaloids, 5 alkaloids were reported for the first time for this plant. Several compounds with pharmacological activity were found. Haemanthamine was main alkaloid in the leaves and bulbs whereas galanthane was found to be main alkaloid in roots.
Subject(s)
Alkaloids/chemistry , Magnoliopsida/chemistry , Alkaloids/isolation & purification , Alkaloids/metabolism , Bulgaria , Gas Chromatography-Mass Spectrometry , Plant Leaves/chemistry , Plant Roots/chemistry , Plants, Medicinal/chemistryABSTRACT
Erucifoline (1) and 9-angeloylhastanecine (2) were isolated from the aerial parts of Senecio aquaticus. Five other pyrrolizidine alkaloids were tentatively identified by GC/MS analysis: jacobine; seneciphylline; spartioidine; jacozine; and senecionine.