[GIS: CAPABILITIES OF DATA ANALYSIS OF PHENO- AND GENOTYPING Of EL TOR 01 SEROGROUP CHOLERA VIBRIOS ISOLATED FROM AQUATIC OBJECTS OF THE ENVIRONMENT IN RUSSIA FEDERATION].

AIM: Application of the authors' GIS <> for systematization of atoxigenic strains ofserogroup 01 choleravibrios (ctxAB-tcpA-, ctxAB-tcpA+), isolated from aquatic objects of the environment by pheno- and genotype. MATERIALS AND METHODS: A sample of 304 Vibrio cholerae 01 strains was studied. Isolation of 39 genes related to pathogenicity was carried out Discrimination ability of a set of genes was determined by Simpson formula. Cluster analysis was carried out by UPGMA method. RESULTS: Analysis of multi-year data on aquatic V cholerae Ο1 strains in country's subject was carried out using GIS. Apossibility of systematization ofphenotypes of the isolated strains by defined parameters was shown. An experimental program for detectior of presence/lack ofvarious genes and their combinations for genotyping was developed. Conclusion GIS was established to allow to carry out analysis of phenotypes by defined parameters, as well as implement approximate systematization of genotypes of atoxigenic strains of cholera vibrios Ο1 by optimally sufficient detection of 14 genes.

[Serotyping and genotypic characteristic of Vibrio cholerae non-O1/non-O139 serogroups isolated from water of surface basins and sewages of Rostov-on-Don city in 2003 - 2008].

AIM: Determination of serogroup and PCR-genotyping of Vibrio cholerae non-O1/non-O139 strains isolated from surface basins and sewages of Rostov-on-Don city in 2003 - 2008. MATERIALS AND METHODS: Seven hundred strains of V. cholerae non-O1/non-O139 serogroups were studied in reaction of slide-agglutination with array of 80 diagnostic sera for non-O1/non-O139 serogroups. Selective screening of strains representing dominating serogroups was performed for extended number of genetic determinants of pathogenicity factors. RESULTS: It was established that V. cholerae belonging to serogroups O53, O67, O75, and O76 are dominating in water ecosystems of Rostov-on-Don city at this time. All studied strains were characterized by lack of cholera toxin genes and toxin-coregulated pili but had different combinations of genes of additional virulence factors. There was no correlation between genotypic characteristics and serogroup. CONCLUSION: The study showed that change of serologic landscape of V. cholerae non-O1/non-O139 occurred in water objects in studied area during last decades. Necessity of dynamic surveillance for circulation of V. cholerae non-O1/non-O139 in aquatic environment with widening of studied spectrum of their biological features was demonstrated.