ABSTRACT
The aim of this study was to develop a resynchronization strategy before the return of oestrus in cows diagnosed as not pregnant after fixed-time artificial insemination (TAI). A total of 839 cows, approximately 45 days post-partum, were synchronized using TAI. On day 0, intravaginal progesterone-releasing devices were inserted and 2 mg of oestradiol benzoate was administered. Eight days later (D8), the progesterone-releasing devices were removed and oestradiol cypionate (0.5 mg, eCG [300 IU]) and prostaglandin (7.5 mg) were administered. All cows were inseminated between 48 and 56 hr after device removal (D10). Thirty days after TAI, cows that were not diagnosed as pregnant by ultrasound were immediately resynchronized and again inseminated at a fixed time. The hormonal protocol used in the first and second rounds of TAI was the same. The pregnancy rate after the first TAI was 52%, and after the second TAI, it was 49%. The increase in the total pregnancy rate (synchronization + second oestrous synchronization) compared to a single synchronization was 23.5%. In conclusion, resynchronization of oestrus and ovulation in zebu cows that had previously undergone TAI protocols is effective in increasing the reproductive efficiency.