ABSTRACT
OBJECTIVE: To investigate the correlation between plasmacytoid dendritic cell (pDC) dose in grafts and the occurrence of cytomegalovirus (CMV) infection after allogeneic hematopoietic stem cell transplantation (allo-HSCT). METHODS: The clinical data of 80 children who received allo-HSCT in Children's Hospital of Soochow University from August 20, 2020 to June 11, 2021 were retrospectively analyzed. Proportions of DC subsets and T-cell subsets in grafts were detected by flow cytometry in order to calculate infused cell dose of each cell. Weekly monitoring of CMV-DNA copies in peripheral blood for each child were performed after transplantation. The last follow-up date was December 31, 2021. RESULTS: All the children gained hematopoietic reconstitution. CMV infection was observed in 51 children (63.8%±5.4%) within the first 100 days after transplantation, including 2 cases developing CMV disease. Univariate analysis indicated that infused doses of DC and pDC were significantly associated with CMV infection within 100 days after allo-HSCT (P <0.05). Multivariate analysis indicated that a high dose infusion of pDC was an independent protective factor for CMV infection within 100 days after allo-HSCT (P <0.05). By the end of follow-up, 7 children died of transplantation-related complications, including 2 deaths from CMV disease, 2 deaths from extensive chronic graft-versus-host disease, and 3 deaths from capillary leak syndrome. The overall survival rate was 91.2%. CONCLUSION: The pDC in grafts may be associated with early infection of CMV after allo-HSCT, while a high infused pDC dose may serve as a protective factor for CMV infection after transplantation.
Subject(s)
Cytomegalovirus Infections , Graft vs Host Disease , Hematopoietic Stem Cell Transplantation , Child , Humans , Retrospective Studies , Graft vs Host Disease/complications , Hematopoietic Stem Cell Transplantation/adverse effects , Dendritic CellsABSTRACT
Two supramolecular assemblies were developed by the dimmolybdenum paddlewheel complex trans-Mo(2)(DAniF)(2)(O(2)CC(6)F(5))(2) (1) (ancillary ligand DAniF = N,N'-di-(p-anisyl)formamidinate) through intermolecular offset face to face C(6)F(5)···C(6)F(5) interactions. The two networks are different because of the presence and absence of tetrahydrofuran (THF) axial coordination to the Mo(2) units, but feature commonly short interplanar distances between the two paired perfluorophenyl groups, that is, 3.30 Å and 3.23 Å, respectively, which are similar to the shortest intermolecular C···F contacts between two atoms in meta and ortho positions. Consistently, as indicated by the Mulliken population analysis, the dipole moments for the meta and ortho C-F bonds are considerably enlarged because the pentafluorobenzoate groups are bonded to the dimetal unit. In comparison, X-ray structural and theoretical analyses were also made for the parent molecule pentafluorobenzoic acid. The resultant data show that charge distribution on the perfluorophenyl group has a major influence on the C(6)F(5)···C(6)F(5) binding mode. Therefore, it is evidenced that the dimetal unit plays a role in further polarizing the highly polarized C-F bonds and the intermolecular perfluorophenyl-perfluorophenyl interactions are dominated by the C···F dipole-dipole interaction, rather than aromatic-aromatic π-π stacking.
ABSTRACT
A simple and efficient synthesis of N-sulfonyl ketimines through a Pd-catalyzed cross-coupling reaction between organoboronic acids and tosylbenzimidoyl chlorides under mild conditions has been developed.
