Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.

Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm. TAM 111 and TAM 112 are two popular cultivars grown in the Southern US Great Plains with significantly different protein content. To investigate regulatory differences, transcriptome data were analyzed from developing grains at early- and mid-filling stages. At the mid-filling stage, TAM 111 preferentially upregulated starch metabolism-related pathways compared to TAM 112, whereas amino acid metabolism and transporter-related pathways were over-represented in TAM 112. Elemental analyses also indicated a higher N percentage in TAM 112 at the mid-filling stage. To explore the regulatory variation, weighted correlation gene network was constructed from publicly available RNAseq datasets to identify the modules differentially regulated in TAM 111 and TAM 112. Further, the potential transcription factors (TFs) regulating those modules were identified using graphical least absolute shrinkage and selection operator (GLASSO). Homologs of the OsNF-Y family members with known starch metabolism-related functions showed higher connectivities in TAM 111. Multiple TFs with high connectivity in TAM 112 had predicted functions associated with ABA response in grain. These results will provide novel targets for breeders to explore and further our understanding in mechanisms regulating grain development.

Tissue specific expression of UMAMIT amino acid transporters in wheat.

Wheat grain protein content and composition are important for its end-use quality. Protein synthesis during the grain filling phase is supported by the amino acids remobilized from the vegetative tissue, the process in which both amino acid importers and exporters are expected to be involved. Previous studies identified amino acid importers that might function in the amino acid remobilization in wheat. However, the amino acid exporters involved in this process have been unexplored so far. In this study, we have curated the Usually Multiple Amino acids Move In and out Transporter (UMAMIT) family of transporters in wheat. As expected, the majority of UMAMITs were found as triads in the A, B, and D genomes of wheat. Expression analysis using publicly available data sets identified groups of TaUMAMITs expressed in root, leaf, spike, stem and grain tissues, many of which were temporarily regulated. Strong expression of TaUMAMITs was detected in the late senescing leaves and transfer cells in grains, both of which are the expected site of apoplastic amino acid transport during grain filling. Biochemical characterization of selected TaUMAMITs revealed that TaUMAMIT17 shows a strong amino acid export activity and might play a role in amino acid transfer to the grains.

Effect of ethyl acetate extract of Songaricum Rupron on p38 and CREB characteristics of ovariectomized rats / 中国药学杂志

OBJECTIVE: To study the effect of ethyl acetate extract of Songaricum Rupron (ECS) on ovariectomization induced learning and memory disorder in rats and its potential mechanism. METHODS: SD rats were ovariectomized to establish animal model of learning and memory disorder, and taken ECS by intragastric administration(3.3 mg · kg-1). Morris water maze was used to test the learning and memory ability of rats. HE staining was used to observe morphological change of hippocampal neurons. The p38 and p-CREB expression were tested by Western-Blot. RESULTS: The latent route of OVX + ECS group in the Morris water maze was significantly shorten, while numbers of crossing platform were markedly increased, compared with OVX group. The neurological form of pyramidal cells in hippocampus were improved by HE staining. CONCLUSION: It shows that the p38 expression is reduced while p-CREB is increased in OVX + ECS group, which potentially contributes to the improvement of learning and memory disorder due to lack of endogenous estrogen.