A novel function of the tomato CALCINEURIN-B LIKE 10 gene as a root-located negative regulator of salt stress.

Climate change exacerbates abiotic stresses like salinization, negatively impacting crop yield, so development of strategies, like using salt-tolerant rootstocks, is crucial. The CALCINEURIN B-LIKE 10 (SlCBL10) gene has been previously identified as a positive regulator of salt tolerance in the tomato shoot. Here, we report a different function of SlCBL10 in tomato shoot and root, as disruption of SlCBL10 only induced salt sensitivity when it was used in the scion but not in the rootstock. The use of SlCBL10 silencing rootstocks (Slcbl10 mutant and RNAi line) improved salt tolerance on the basis of fruit yield. These changes were associated with improved Na+ and K+ homoeostasis, as SlCBL10 silencing reduced the Na+ content and increased the K+ content under salinity, not only in the rootstock but also in the shoot. Improvement of Na+ homoeostasis in Slcbl10 rootstock seems to be mainly due to induction of SlSOS1 expression, while the higher K+ accumulation in roots seems to be mainly determined by expression of LKT1 transporter and SlSKOR channel. These findings demonstrate that SlCBL10 is a negative regulator of salt tolerance in the root, so the use of downregulated SlCBL10 rootstocks may provide a suitable strategy to increase tomato fruit production under salinity.

Salt-tolerant alternative crops as sources of quality food to mitigate the negative impact of salinity on agricultural production.

An increase of abiotic stress tolerance and nutritive value of foods is currently a priority because of climate change and rising world population. Among abiotic stresses, salt stress is one of the main problems in agriculture. Mounting urbanization and industrialization, and increasing global food demand, are pressing farmers to make use of marginal lands affected by salinity and low-quality saline water. In that situation, one of the most promising approaches is searching for new sources of genetic variation like salt-tolerant alternative crops or underexploited crops. They are generally less efficient than cultivated crops in optimal conditions due to lower yield but represent an alternative in stressful growth conditions. In this review, we summarize the advances achieved in research on underexploited species differing in their genetic nature. First, we highlight advances in research on salt tolerance of traditional varieties of tomato or landraces; varieties selected and developed by smallholder farmers for adaptation to their local environments showing specific attractive fruit quality traits. We remark advances attained in screening a collection of tomato traditional varieties gathered in Spanish Southeast, a very productive region which environment is extremely stressing. Second, we explore the opportunities of exploiting the natural variation of halophytes, in particular quinoa and amaranth. The adaptation of both species in stressful growth conditions is becoming an increasingly important issue, especially for their cultivation in arid and semiarid areas prone to be affected by salinity. Here we present a project developed in Spanish Southeast, where quinoa and amaranth varieties are being adapted for their culture under abiotic stress targeting high quality grain.