Larvicidal activity of Caesalpinia ferrea Mart. and Lippia origanoides Cham. Against Aedes aegypti (LINNAEUS, 1762) (Diptera: Culicidae)

The control of Aedes aegypti has been considered one of the most important public health challenges worldwide. Chemical compounds have long been used for this purpose, but resistance to these molecules has also increased. Therefore, over the last few years several studies have focused on the development of alternative tools, particularly those based on plant metabolites. The purpose of this study was to assess the larvicidal activity of Caesalpinia ferrea and Lippia origanoides against Ae. aegypti. Larvae (L3) of Ae. aegypti Liverpool and Rockefeller strains, as well as of the Recife population were exposed to different concentrations of C. ferrea (ranging from 13.1 to 105 mg/mL) and L. origanoides (ranging from 16.3 to 130 mg/mL), and the mortality rate was evaluated up to 48 hours after the beginning of the experiment. All tested groups and ontrol group were quadruplicated. For C. ferrea, mortality ranged from 42.5% to 100% for Ae. aegypti Liverpool strain, from 67% to 100% for Ae. aegypti Rockfeller strain, and 57% to 100% for Ae. aegypti Recife population after 48 hours of larval exposure. For L. origanoides, the larvicidal activity ranged from 75% to 100% for Ae. aegypti Liverpool strain, from 61.5% to 100% for Ae. aegypti Rockfeller strain, and from 60.5% to 100% for Ae. aegypti Recife population. The hydro ethanol extract of C. ferrea and L. origanoides presented larvicidal activity against Ae. aegypti.

Sandflies in an endemic area for Visceral Leishmaniasis in Northeastern Brazil / Flebotomíneos em uma área endêmica para Leishmaniose Visceral no Nordeste do Brasil

Abstract Phlebotomines of the genus Lutzomyia are important vector for species of Leishmania, the etiological agent of leishmaniasis. The aim of this study was to assess the diversity of sandflies in an endemic area for Visceral Leishmaniasis (VL). Sampling was performed in four localities on the coast of the municipality of Goiana, state of Pernambuco, Northeastern Brazil. Specimens were collected monthly on three consecutive nights, from November 2017 to October 2018. CDC light traps were installed at a height of 1.5 m above the ground, in two ecotypes (i.e. tree and animal facility), both in peridomestic areas. A total of 5,205 sandflies were collected, among which the species Lutzomyia longipalpis (99.85%; 5,197/5,205) was the most common, followed by Lutzomyia evandroi (0.13%; 07/5,205) and Lutzomyia whitmani (0.02%; 01/5,205). Specimens of L. longipalpis were collected throughout the study period and most of them were found near to the animal facilities. The findings from this research indicate that vectors for Leishmania infantum are present in the study area throughout the year, along with occurrences of vectors relating to Cutaneous Leishmaniasis (CL). Preventive vector control measures need to be implemented in Goiana to reduce the risk of infection for the human and animal populations.

Resumo Flebotomíneos do gênero Lutzomyia são importantes vetores de espécies de Leishmania, agente etiológico das leishmanioses. O objetivo deste estudo foi avaliar a diversidade de flebotomíneos em área endêmica para Leishmaniose Visceral (LV). As coletas foram realizadas em quatro localidades do litoral do município de Goiana, Pernambuco, Nordeste do Brasil. Os espécimes foram coletados mensalmente em três noites consecutivas de novembro de 2017 a outubro de 2018. Foram instaladas armadilhas luminosas modelo CDC a uma altura de 1,5 m acima do solo em dois ecótopos (ex. árvore e instalações de animais), ambos em áreas peridomiciliares. Um total de 5.205 flebotomíneos foi coletado, sendo Lutzomyia longipalpis (99,85%; 5.197/5.205) a mais comum, seguida por Lutzomyia evandroi (0,13%; 07/5.205) e Lutzomyia whitmani (0,02%; 01/5.205). L. longipalpis foi coletada durante todo o período do estudo. A maioria dos espécimes foi detectada perto das instalações dos animais. Os achados deste estudo indicam a presença de vetores de Leishmania infantum na área avaliada ao longo do ano, bem como a ocorrência de vetores relacionados à Leishmaniose Cutânea (LC). Medidas preventivas de controle vetorial devem ser implementadas em Goiana para reduzir o risco de infecção à população humana e animal.

Sandflies in an endemic area for Visceral Leishmaniasis in Northeastern Brazil.

Phlebotomines of the genus Lutzomyia are important vector for species of Leishmania, the etiological agent of leishmaniasis. The aim of this study was to assess the diversity of sandflies in an endemic area for Visceral Leishmaniasis (VL). Sampling was performed in four localities on the coast of the municipality of Goiana, state of Pernambuco, Northeastern Brazil. Specimens were collected monthly on three consecutive nights, from November 2017 to October 2018. CDC light traps were installed at a height of 1.5 m above the ground, in two ecotypes (i.e. tree and animal facility), both in peridomestic areas. A total of 5,205 sandflies were collected, among which the species Lutzomyia longipalpis (99.85%; 5,197/5,205) was the most common, followed by Lutzomyia evandroi (0.13%; 07/5,205) and Lutzomyia whitmani (0.02%; 01/5,205). Specimens of L. longipalpis were collected throughout the study period and most of them were found near to the animal facilities. The findings from this research indicate that vectors for Leishmania infantum are present in the study area throughout the year, along with occurrences of vectors relating to Cutaneous Leishmaniasis (CL). Preventive vector control measures need to be implemented in Goiana to reduce the risk of infection for the human and animal populations.

[Epistemological analysis of the scientific knowledge about Toxocara sp. with the emphasis on human infection]. / Análise epistemológica dos conhecimentos científicos sobre Toxocara sp. com ênfase na infecção humana.

The scope of this article was to analyze the evolution of knowledge about Toxocara sp. from a zoonotic point of view by means of scientific data published between the years 1996 and 2015 using epistemology as a tool. The epistemological analysis of the evolution of knowledge on the zoonotic aspect of Toxocara sp. in Brazil revealed that, in general, little new information was added which had, over the period studied, slow and discontinuous absorption, and was not incorporated in the social reality from the standpoint of public health.

Este artigo objetivou analisar a evolução do conhecimento sobre Toxocara sp. em seu aspecto zoonótico por meio de dados científicos entre os anos de 1996 a 2015 utilizando a epistemologia como ferramenta. A análise epistemológica da evolução do conhecimento sobre o aspecto zoonótico de Toxocara sp. no Brasil demonstrou que, de uma maneira geral, foram agregadas poucas novas informações as quais tiveram, ao longo do período estudado, absorção lenta, descontínua, e não foram inseridas na realidade social do ponto de vista de Saúde Pública.

Sandflies in an endemic area for Visceral Leishmaniasis in Northeastern Brazil / Flebotomíneos em uma área endêmica para Leishmaniose Visceral no Nordeste do Brasil

Phlebotomines of the genus Lutzomyia are important vector for species of Leishmania, the etiological agent of leishmaniasis. The aim of this study was to assess the diversity of sandflies in an endemic area for Visceral Leishmaniasis (VL). Sampling was performed in four localities on the coast of the municipality of Goiana, state of Pernambuco, Northeastern Brazil. Specimens were collected monthly on three consecutive nights, from November 2017 to October 2018. CDC light traps were installed at a height of 1.5 m above the ground, in two ecotypes (i.e. tree and animal facility), both in peridomestic areas. A total of 5,205 sandflies were collected, among which the species Lutzomyia longipalpis (99.85%; 5,197/5,205) was the most common, followed by Lutzomyia evandroi (0.13%; 07/5,205) and Lutzomyia whitmani (0.02%; 01/5,205). Specimens of L. longipalpis were collected throughout the study period and most of them were found near to the animal facilities. The findings from this research indicate that vectors for Leishmania infantum are present in the study area throughout the year, along with occurrences of vectors relating to Cutaneous Leishmaniasis (CL). Preventive vector control measures need to be implemented in Goiana to reduce the risk of infection for the human and animal populations.(AU)

Flebotomíneos do gênero Lutzomyia são importantes vetores de espécies de Leishmania, agente etiológico das leishmanioses. O objetivo deste estudo foi avaliar a diversidade de flebotomíneos em área endêmica para Leishmaniose Visceral (LV). As coletas foram realizadas em quatro localidades do litoral do município de Goiana, Pernambuco, Nordeste do Brasil. Os espécimes foram coletados mensalmente em três noites consecutivas de novembro de 2017 a outubro de 2018. Foram instaladas armadilhas luminosas modelo CDC a uma altura de 1,5 m acima do solo em dois ecótopos (ex. árvore e instalações de animais), ambos em áreas peridomiciliares. Um total de 5.205 flebotomíneos foi coletado, sendo Lutzomyia longipalpis (99,85%; 5.197/5.205) a mais comum, seguida por Lutzomyia evandroi (0,13%; 07/5.205) e Lutzomyia whitmani (0,02%; 01/5.205). L. longipalpis foi coletada durante todo o período do estudo. A maioria dos espécimes foi detectada perto das instalações dos animais. Os achados deste estudo indicam a presença de vetores de Leishmania infantum na área avaliada ao longo do ano, bem como a ocorrência de vetores relacionados à Leishmaniose Cutânea (LC). Medidas preventivas de controle vetorial devem ser implementadas em Goiana para reduzir o risco de infecção à população humana e animal.(AU)

Análise epistemológica dos conhecimentos científicos sobre Toxocara sp. com ênfase na infecção humana / Epistemological analysis of the scientific knowledge about Toxocara sp. with the emphasis on human infection

Resumo Este artigo objetivou analisar a evolução do conhecimento sobre Toxocara sp. em seu aspecto zoonótico por meio de dados científicos entre os anos de 1996 a 2015 utilizando a epistemologia como ferramenta. A análise epistemológica da evolução do conhecimento sobre o aspecto zoonótico de Toxocara sp. no Brasil demonstrou que, de uma maneira geral, foram agregadas poucas novas informações as quais tiveram, ao longo do período estudado, absorção lenta, descontínua, e não foram inseridas na realidade social do ponto de vista de Saúde Pública.

Abstract The scope of this article was to analyze the evolution of knowledge about Toxocara sp. from a zoonotic point of view by means of scientific data published between the years 1996 and 2015 using epistemology as a tool. The epistemological analysis of the evolution of knowledge on the zoonotic aspect of Toxocara sp. in Brazil revealed that, in general, little new information was added which had, over the period studied, slow and discontinuous absorption, and was not incorporated in the social reality from the standpoint of public health.

Detection and molecular characterization of Trypanosoma (Duttonella) vivax in dairy cattle in the state of Sergipe, northeastern Brazil / Detecção e caracterização molecular de Trypanosoma (Duttonella) vivax em gado leiteiro no estado de Sergipe, no Nordeste do Brasil

Trypanosoma (Duttonella) vivax is an important cause of economic losses among feedlot cattle. These losses are related to the morbidity, mortality, reproductive issues and decreased production. It is known that the clinical signs observed in infections by this protozoon are similar to other hemoparasitosis, which difficult the diagnosis. Therefore, the aim of this study was to detect and molecularly characterize an outbreak of trypanosomiasis caused by T. (D.) vivax in dairy cattle in the municipality of São Miguel Aleixo, state of Sergipe, Brazil. Blood samples from cattle (n = 15) presenting clinical signs compatible with trypanosomiasis were collected and parasitological and molecular evaluated. Among the samples analyzed, 34% (5/15) were positive from blood smears, 60% (9/15) from the buffy coat method and 80% (12/15) from the molecular method. The DNA sequence obtained (659 bp) showed 99% similarity to T. (D.) vivax sequences that are available in the GenBank database. The presence of this protozoon in cattle herds is a problem for producers. Diagnosing trypanosomiasis is problematic because its evolution is similar to that of other parasitic blood diseases. In addition, this is the first report of infection by T. (D.) vivax in cattle in the state of Sergipe, northeastern Brazil.(AU)

Trypanosoma (Duttonella) vivax é responsável por consideráveis perdas econômicas na bovinocultura. Estas perdas estão relacionados à morbidade, mortalidade, problemas reprodutivos e declínio na produção. Sabe-se que os sinais clínicos apresentados em infecções por este protozoário se assemelha a outras hemoparasitoses, dificultando muitas vezes o diagnóstico. Portanto, objetivou-se com este estudo detectar a ocorrência de T. (D.) vivax em bovinos leiteiros no município de São Miguel Aleixo, Estado de Sergipe, Brasil. Para tanto, amostras de sangue (n = 15) foram coletadas e avaliadas através de métodos parasitológicos e moleculares. Do total das amostras analisadas, 34% (5/15) foram positivas no esfregaço sanguíneo, 60% (9/15) pelo método do Buffy Coat, enquanto na biologia molecular 80% (12/15) amplificaram um fragmento de DNA (659 pb) compatível com T. (D.) vivax (GenBank). Em conclusão a presença de T. (D.) vivax nos rebanhos bovinos caracteriza-se como um problema para os pecuaristas, como também para o diagnóstico, uma vez que essa tripanossomíase apresenta evolução semelhante a outras hemoparasitoses. Ademais, este é o primeiro relato de infecção por T. (D.) vivax em bovinos do estado de Sergipe, nordeste do Brasil.(AU)

Detection and molecular characterization of Trypanosoma (Duttonella) vivax in dairy cattle in the state of Sergipe, northeastern Brazil / Detecção e caracterização molecular de Trypanosoma (Duttonella) vivax em gado leiteiro no estado de Sergipe, no Nordeste do Brasil

Abstract Trypanosoma (Duttonella) vivax is an important cause of economic losses among feedlot cattle. These losses are related to the morbidity, mortality, reproductive issues and decreased production. It is known that the clinical signs observed in infections by this protozoon are similar to other hemoparasitosis, which difficult the diagnosis. Therefore, the aim of this study was to detect and molecularly characterize an outbreak of trypanosomiasis caused by T. (D.) vivax in dairy cattle in the municipality of São Miguel Aleixo, state of Sergipe, Brazil. Blood samples from cattle (n = 15) presenting clinical signs compatible with trypanosomiasis were collected and parasitological and molecular evaluated. Among the samples analyzed, 34% (5/15) were positive from blood smears, 60% (9/15) from the buffy coat method and 80% (12/15) from the molecular method. The DNA sequence obtained (659 bp) showed 99% similarity to T. (D.) vivax sequences that are available in the GenBank database. The presence of this protozoon in cattle herds is a problem for producers. Diagnosing trypanosomiasis is problematic because its evolution is similar to that of other parasitic blood diseases. In addition, this is the first report of infection by T. (D.) vivax in cattle in the state of Sergipe, northeastern Brazil.

Resumo Trypanosoma (Duttonella) vivax é responsável por consideráveis perdas econômicas na bovinocultura. Estas perdas estão relacionados à morbidade, mortalidade, problemas reprodutivos e declínio na produção. Sabe-se que os sinais clínicos apresentados em infecções por este protozoário se assemelha a outras hemoparasitoses, dificultando muitas vezes o diagnóstico. Portanto, objetivou-se com este estudo detectar a ocorrência de T. (D.) vivax em bovinos leiteiros no município de São Miguel Aleixo, Estado de Sergipe, Brasil. Para tanto, amostras de sangue (n = 15) foram coletadas e avaliadas através de métodos parasitológicos e moleculares. Do total das amostras analisadas, 34% (5/15) foram positivas no esfregaço sanguíneo, 60% (9/15) pelo método do Buffy Coat, enquanto na biologia molecular 80% (12/15) amplificaram um fragmento de DNA (659 pb) compatível com T. (D.) vivax (GenBank). Em conclusão a presença de T. (D.) vivax nos rebanhos bovinos caracteriza-se como um problema para os pecuaristas, como também para o diagnóstico, uma vez que essa tripanossomíase apresenta evolução semelhante a outras hemoparasitoses. Ademais, este é o primeiro relato de infecção por T. (D.) vivax em bovinos do estado de Sergipe, nordeste do Brasil.

Detection and molecular characterization of Trypanosoma (Duttonella) vivax in dairy cattle in the state of Sergipe, northeastern Brazil.

Trypanosoma (Duttonella) vivax is an important cause of economic losses among feedlot cattle. These losses are related to the morbidity, mortality, reproductive issues and decreased production. It is known that the clinical signs observed in infections by this protozoon are similar to other hemoparasitosis, which difficult the diagnosis. Therefore, the aim of this study was to detect and molecularly characterize an outbreak of trypanosomiasis caused by T. (D.) vivax in dairy cattle in the municipality of São Miguel Aleixo, state of Sergipe, Brazil. Blood samples from cattle (n = 15) presenting clinical signs compatible with trypanosomiasis were collected and parasitological and molecular evaluated. Among the samples analyzed, 34% (5/15) were positive from blood smears, 60% (9/15) from the buffy coat method and 80% (12/15) from the molecular method. The DNA sequence obtained (659 bp) showed 99% similarity to T. (D.) vivax sequences that are available in the GenBank database. The presence of this protozoon in cattle herds is a problem for producers. Diagnosing trypanosomiasis is problematic because its evolution is similar to that of other parasitic blood diseases. In addition, this is the first report of infection by T. (D.) vivax in cattle in the state of Sergipe, northeastern Brazil.

Gastrointestinal parasites of exotic birds living in captivity in the state of Sergipe, Northeastern Brazil / Parasitos gastrointestinais de aves exóticas que vivem em cativeiro no estado de Sergipe, Nordeste do Brasil

Brazil has a wide diversity of exotic birds that were brought to this country during the European colonization. These animals are kept in captivity and, in some cases, in inadequate facilities, which may facilitate the introduction of pathogens, including gastrointestinal parasites. The purpose of this study was to identify the main gastrointestinal parasites that affect exotic birds living in captivity in the state of Sergipe, Northeastern Brazil. Fecal samples (n = 362) from exotic birds were analyzed by direct examination and by the Willis-Mollay technique. Eggs of Amidostomum sp., Ascaridia sp., Capillarinae, Heterakis sp., Trichostrongylidae, and oocysts of coccidians were identified in nine different bird species. This is the first report in Brazil of infection by Ascaridia sp. and Heterakis sp. in Nymphicus hollandicus; by Trichostrongylidae in Serinus canaria and Streptopelia risoria.(AU)

O Brasil possui uma grande diversidade de aves exóticas que foram trazidas para o país durante a colonização europeia. Estes animais são mantidos em cativeiros, os quais muitas vezes são inadequados, proporcionando o surgimento de doenças provocadas por parasitas gastrointestinais. Objetivou-se neste estudo, realizar avaliação coproparasitológica de fezes de aves ornamentais mantidas em cativeiros no estado de Sergipe, região Nordeste do Brasil. Amostras fecais (n= 362) de aves exóticas foram analisadas através do exame direto a fresco e técnica de Willis-Mollay. Ovos de Amidostomum sp., Ascaridia sp., Capillarinae, Heterakis sp., Trichostrongylidae, e oocistos de coccídeos foram encontrados em nove diferentes espécies de aves. Aqui, destacamos, os primeiros registros no Brasil de Ascaridia sp. e Heterakis sp., parasitando Nymphicus hollandicus; Trichostrongilidae em Serinus canaria e Streptopelia risoria.(AU)

Gastrointestinal parasites of exotic birds living in captivity in the state of Sergipe, Northeastern Brazil / Parasitos gastrointestinais de aves exóticas que vivem em cativeiro no estado de Sergipe, Nordeste do Brasil

Abstract Brazil has a wide diversity of exotic birds that were brought to this country during the European colonization. These animals are kept in captivity and, in some cases, in inadequate facilities, which may facilitate the introduction of pathogens, including gastrointestinal parasites. The purpose of this study was to identify the main gastrointestinal parasites that affect exotic birds living in captivity in the state of Sergipe, Northeastern Brazil. Fecal samples (n = 362) from exotic birds were analyzed by direct examination and by the Willis-Mollay technique. Eggs of Amidostomum sp., Ascaridia sp., Capillarinae, Heterakis sp., Trichostrongylidae, and oocysts of coccidians were identified in nine different bird species. This is the first report in Brazil of infection by Ascaridia sp. and Heterakis sp. in Nymphicus hollandicus; by Trichostrongylidae in Serinus canaria and Streptopelia risoria.

Resumo O Brasil possui uma grande diversidade de aves exóticas que foram trazidas para o país durante a colonização europeia. Estes animais são mantidos em cativeiros, os quais muitas vezes são inadequados, proporcionando o surgimento de doenças provocadas por parasitas gastrointestinais. Objetivou-se neste estudo, realizar avaliação coproparasitológica de fezes de aves ornamentais mantidas em cativeiros no estado de Sergipe, região Nordeste do Brasil. Amostras fecais (n= 362) de aves exóticas foram analisadas através do exame direto a fresco e técnica de Willis-Mollay. Ovos de Amidostomum sp., Ascaridia sp., Capillarinae, Heterakis sp., Trichostrongylidae, e oocistos de coccídeos foram encontrados em nove diferentes espécies de aves. Aqui, destacamos, os primeiros registros no Brasil de Ascaridia sp. e Heterakis sp., parasitando Nymphicus hollandicus; Trichostrongilidae em Serinus canaria e Streptopelia risoria.

Gastrointestinal parasites of exotic birds living in captivity in the state of Sergipe, Northeastern Brazil.

Brazil has a wide diversity of exotic birds that were brought to this country during the European colonization. These animals are kept in captivity and, in some cases, in inadequate facilities, which may facilitate the introduction of pathogens, including gastrointestinal parasites. The purpose of this study was to identify the main gastrointestinal parasites that affect exotic birds living in captivity in the state of Sergipe, Northeastern Brazil. Fecal samples (n = 362) from exotic birds were analyzed by direct examination and by the Willis-Mollay technique. Eggs of Amidostomum sp., Ascaridia sp., Capillarinae, Heterakis sp., Trichostrongylidae, and oocysts of coccidians were identified in nine different bird species. This is the first report in Brazil of infection by Ascaridia sp. and Heterakis sp. in Nymphicus hollandicus; by Trichostrongylidae in Serinus canaria and Streptopelia risoria.

Gastrointestinal parasites of cats in Brazil: frequency and zoonotic risk / Parasitas gastrointestinais em gatos no Brasil: frequência e risco zoonótico

Gastrointestinal helminths are considered to be the most common parasites affecting cats worldwide. Correct diagnosis of these parasites in animals living in urban areas is pivotal, especially considering the zoonotic potential of some species (e.g. Ancylostoma sp. and Toxocara sp.). In this study, a copromicroscopic survey was conducted using fecal samples (n = 173) from domestic cats living in the northeastern region of Brazil. Samples were examined through the FLOTAC technique and the overall results showed positivity of 65.31% (113/173) among the samples analyzed. Coinfections were observed in 46.01% (52/113) of the positive samples. The most common parasites detected were Ancylostoma sp., Toxocara cati, Strongyloides stercoralis, Trichuris sp., Dipylidium caninum and Cystoisospora sp. From an epidemiological point of view, these findings are important, especially considering that zoonotic parasites (e.g. Ancylostoma sp. and Toxocara sp.) were the nematodes most frequently diagnosed in this study. Therefore, the human population living in close contact with cats is at risk of infection caused by the zoonotic helminths of these animals. In addition, for the first time the FLOTAC has been used to diagnosing gastrointestinal parasites of cats in Brazil.(AU)

Helmintos gastrointestinais são considerados os mais frequentes parasitos que afetam gatos em todo o mundo. O correto diagnóstico desses parasitos, em animais que vivem em áreas urbanas, é crucial, especialmente quando considerado o potencial zoonótico de algumas espécies (ex. Ancylostoma sp. e Toxocara sp.). Neste estudo, uma pesquisa coproparasitológica foi realizada, utilizando-se amostras de fezes (n = 173) de gatos domésticos da região Nordeste do Brasil. As amostras foram examinadas através da técnica FLOTAC, e os resultados gerais mostraram uma positividade de 65,31% (113/173). Co-infecções foram observadas em 46,01% (52/113) das amostras positivas. Os parasitas mais comuns aqui detectados foram Ancylostoma sp., Toxocara cati, Strongyloides stercoralis, Trichuris sp., Dipylidium caninum e Cystoisospora sp. De um ponto de vista epidemiológico, esses resultados são importantes, especialmente considerando que os parasitos zoonóticos (ex. Ancylostoma sp. e Toxocara sp.) foram os mais frequentes nematódeos diagnosticados neste estudo. Portanto, a população humana, em estreito convívio com esses animais, apresenta risco de infecção por helmintos de caráter zoonótico. Além disso, pela primeira vez, a técnica de FLOTAC tem sido utilizada no diagnóstico de parasitos gastrointestinais em gatos no Brasil.(AU)

Gastrointestinal parasites of cats in Brazil: frequency and zoonotic risk / Parasitas gastrointestinais em gatos no Brasil: frequência e risco zoonótico

Abstract Gastrointestinal helminths are considered to be the most common parasites affecting cats worldwide. Correct diagnosis of these parasites in animals living in urban areas is pivotal, especially considering the zoonotic potential of some species (e.g. Ancylostoma sp. and Toxocara sp.). In this study, a copromicroscopic survey was conducted using fecal samples (n = 173) from domestic cats living in the northeastern region of Brazil. Samples were examined through the FLOTAC technique and the overall results showed positivity of 65.31% (113/173) among the samples analyzed. Coinfections were observed in 46.01% (52/113) of the positive samples. The most common parasites detected were Ancylostoma sp., Toxocara cati, Strongyloides stercoralis, Trichuris sp., Dipylidium caninum and Cystoisospora sp. From an epidemiological point of view, these findings are important, especially considering that zoonotic parasites (e.g. Ancylostoma sp. and Toxocara sp.) were the nematodes most frequently diagnosed in this study. Therefore, the human population living in close contact with cats is at risk of infection caused by the zoonotic helminths of these animals. In addition, for the first time the FLOTAC has been used to diagnosing gastrointestinal parasites of cats in Brazil.

Resumo Helmintos gastrointestinais são considerados os mais frequentes parasitos que afetam gatos em todo o mundo. O correto diagnóstico desses parasitos, em animais que vivem em áreas urbanas, é crucial, especialmente quando considerado o potencial zoonótico de algumas espécies (ex. Ancylostoma sp. e Toxocara sp.). Neste estudo, uma pesquisa coproparasitológica foi realizada, utilizando-se amostras de fezes (n = 173) de gatos domésticos da região Nordeste do Brasil. As amostras foram examinadas através da técnica FLOTAC, e os resultados gerais mostraram uma positividade de 65,31% (113/173). Co-infecções foram observadas em 46,01% (52/113) das amostras positivas. Os parasitas mais comuns aqui detectados foram Ancylostoma sp., Toxocara cati, Strongyloides stercoralis, Trichuris sp., Dipylidium caninum e Cystoisospora sp. De um ponto de vista epidemiológico, esses resultados são importantes, especialmente considerando que os parasitos zoonóticos (ex. Ancylostoma sp. e Toxocara sp.) foram os mais frequentes nematódeos diagnosticados neste estudo. Portanto, a população humana, em estreito convívio com esses animais, apresenta risco de infecção por helmintos de caráter zoonótico. Além disso, pela primeira vez, a técnica de FLOTAC tem sido utilizada no diagnóstico de parasitos gastrointestinais em gatos no Brasil.

Gastrointestinal parasites of cats in Brazil: frequency and zoonotic risk.

Gastrointestinal helminths are considered to be the most common parasites affecting cats worldwide. Correct diagnosis of these parasites in animals living in urban areas is pivotal, especially considering the zoonotic potential of some species (e.g. Ancylostoma sp. and Toxocara sp.). In this study, a copromicroscopic survey was conducted using fecal samples (n = 173) from domestic cats living in the northeastern region of Brazil. Samples were examined through the FLOTAC technique and the overall results showed positivity of 65.31% (113/173) among the samples analyzed. Coinfections were observed in 46.01% (52/113) of the positive samples. The most common parasites detected were Ancylostoma sp., Toxocara cati, Strongyloides stercoralis, Trichuris sp., Dipylidium caninum and Cystoisospora sp. From an epidemiological point of view, these findings are important, especially considering that zoonotic parasites (e.g. Ancylostoma sp. and Toxocara sp.) were the nematodes most frequently diagnosed in this study. Therefore, the human population living in close contact with cats is at risk of infection caused by the zoonotic helminths of these animals. In addition, for the first time the FLOTAC has been used to diagnosing gastrointestinal parasites of cats in Brazil.

Use of the FLOTAC technique to diagnosing parasites of the urinary tract of dogs.

Among the nematodes that infect the urinary tract of dogs, the Dioctophyma renale and Capillaria plica are those more frequently reported. For a long time, sedimentation was the only method used to detect eggs of these parasites in urine. The aim of this study was to analyze urine samples (n = 54) of dogs, obtained by bladder catheterization or cystocentesis, by using a modified FLOTAC technique. Animals were divided into two groups, i.e., with (n = 20) and without (n = 34) suspicion of urinary disease. The overall positivity herein observed was 3.8 % (2/54), being all animals (10 %; 2/20) from the group with suspicion of urinary disease. In the first positive sample, a single egg of D. renale was detected, whereas in the second sample two trematode-like eggs were observed. This is the first short survey employed to detect eggs of parasites that inhabit the urinary tract of dogs using a modified FLOTAC technique; in addition, for the first time, eggs of D. renale have been detected using this tool.

New insights into diagnosis of Platynosomum fastosum (Trematoda: Dicrocoeliidae) in cats.

Platynosomum fastosum is a hepatic trematode which causes the so-called lizard poisoning in cats. This parasite is reported in tropical and sub-tropical areas infecting the liver, gallbladder, and bile ducts of cats. Despite its clinical importance, the diagnosis of P. fastosum has been poorly investigated so far. In this study, three cases of infection by P. fastosum in cats are reported. The FLOTAC technique was utilized for the first time in the detection of eggs of this parasite. Three cats (two males and one female), being one of them with a history of diarrhea and vomiting, were diagnosed positive for the presence of P. fastosum eggs through the FLOTAC technique. In conclusion, this study provides important and new insights into the diagnosis of P. fastosum in cats, expanding the geographical knowledge of this parasite in Brazil.

Evaluation of epidemiological and health factors associated with infection by Cryptosporidium spp. in domiciled dogs / Avaliação de fatores epidemiológicos e sanitários associados à infecção por Cryptosporidium spp. em cães domiciliados

The genus Cryptosporidium consists of protozoa with large reproduction and dissemination capacity. Transmission can occur indirectly by ingestion of water contaminated with viable oocysts or directly between animals, between humans, or between animals and human. In various parts of the world, pets have been cited as potential sources of infection by Cryptosporidium spp. The present study aimed to evaluate the occurrence of natural Cryptosporidium spp. infection in domestic dogs in the city of Recife, Pernambuco State, Brazil, as well as the association of the infection with epidemiological and sanitary data. Stool samples from 97 dogs were analyzed using the techniques of centrifugal sedimentation in formalin-ether with subsequent staining by Kinyoun method. Statistical analysis was performed using the chi-square test of Pearson and Fisher Exact test, with significance level of 5.0%. Positivity was detected in 31.9% (31/97) of samples. It was the first record of infection by Cryptosporidium spp. in dogs from northeastern Brazil. We verified significant association between the rate of infection and veterinary medical care, vaccination, and type of environment in which the dogs were kept.(AU)

O gênero Cryptosporidium é composto por protozoários com grande capacidade de reprodução e disseminação. Sua transmissão pode ocorrer indiretamente pela ingestão de água e alimentos contaminados com oocistos viáveis ou diretamente entre animais, entre humanos ou entre animais e humanos. Em diversas partes do mundo, animais de companhia tem sido citados como fontes potenciais de infecção por Cryptosporidium spp. O presente estudo teve como objetivo avaliar a ocorrência da infecção por Cryptosporidium spp. em cães domiciliados na cidade do Recife, estado de Pernambuco, Brasil, bem como a associação da infecção com dados epidemiológicos e sanitários. Foram analisadas 97 amostras de fezes de caninos domiciliados, utilizando-se as técnicas de centrífugo-sedimentação em formol-éter com posterior coloração pelo método de Kinyoun. A análise estatística foi realizada por meio dos testes Qui-quadrado de Pearson e Exato de Fisher, com nível de significância de 5,0%.  Detectou-se positividade de 31,9% (31/97), tratando-se do primeiro registro de ocorrência da infecção por Cryptosporidium spp. na espécie canina no nordeste brasileiro, verificando-se associação significativa entre a taxa de infecção e a assistência médica veterinária, vacinação recebida e o tipo de ambiente de criação dos cães.(AU)

Evaluation of epidemiological and health factors associated with infection by Cryptosporidium spp. in domiciled dogs / Avaliação de fatores epidemiológicos e sanitários associados à infecção por Cryptosporidium spp. em cães domiciliados

The genus Cryptosporidium consists of protozoa with large reproduction and dissemination capacity. Transmission can occur indirectly by ingestion of water contaminated with viable oocysts or directly between animals, between humans, or between animals and human. In various parts of the world, pets have been cited as potential sources of infection by Cryptosporidium spp. The present study aimed to evaluate the occurrence of natural Cryptosporidium spp. infection in domestic dogs in the city of Recife, Pernambuco State, Brazil, as well as the association of the infection with epidemiological and sanitary data. Stool samples from 97 dogs were analyzed using the techniques of centrifugal sedimentation in formalin-ether with subsequent staining by Kinyoun method. Statistical analysis was performed using the chi-square test of Pearson and Fisher Exact test, with significance level of 5.0%. Positivity was detected in 31.9% (31/97) of samples. It was the first record of infection by Cryptosporidium spp. in dogs from northeastern Brazil. We verified significant association between the rate of infection and veterinary medical care, vaccination, and type of environment in which the dogs were kept.

O gênero Cryptosporidium é composto por protozoários com grande capacidade de reprodução e disseminação. Sua transmissão pode ocorrer indiretamente pela ingestão de água e alimentos contaminados com oocistos viáveis ou diretamente entre animais, entre humanos ou entre animais e humanos. Em diversas partes do mundo, animais de companhia tem sido citados como fontes potenciais de infecção por Cryptosporidium spp. O presente estudo teve como objetivo avaliar a ocorrência da infecção por Cryptosporidium spp. em cães domiciliados na cidade do Recife, estado de Pernambuco, Brasil, bem como a associação da infecção com dados epidemiológicos e sanitários. Foram analisadas 97 amostras de fezes de caninos domiciliados, utilizando-se as técnicas de centrífugo-sedimentação em formol-éter com posterior coloração pelo método de Kinyoun. A análise estatística foi realizada por meio dos testes Qui-quadrado de Pearson e Exato de Fisher, com nível de significância de 5,0%.  Detectou-se positividade de 31,9% (31/97), tratando-se do primeiro registro de ocorrência da infecção por Cryptosporidium spp. na espécie canina no nordeste brasileiro, verificando-se associação significativa entre a taxa de infecção e a assistência médica veterinária, vacinação recebida e o tipo de ambiente de criação dos cães.

AVALIAÇÃO DE FATORES EPIDEMIOLÓGICOS E SANITÁRIOS ASSOCIADOS À INFECÇÃO POR Cryptosporidium spp. EM CÃES DOMICILIADOS

Abstract The genus Cryptosporidium consists of protozoa with large reproduction and dissemination capacity. Transmission can occur indirectly by ingestion of water contaminated with viable oocysts or directly between animals, between humans, or between animals and human. In various parts of the world, pets have been cited as potential sources of infection by Cryptosporidium spp. The present study aimed to evaluate the occurrence of natural Cryptosporidium spp. infection in domestic dogs in the city of Recife, Pernambuco State, Brazil, as well as the association of the infection with epidemiological and sanitary data. Stool samples from 97 dogs were analyzed using the techniques of centrifugal sedimentation in formalin-ether with subsequent staining by Kinyoun method. Statistical analysis was performed using the chi-square test of Pearson and Fisher Exact test, with significance level of 5.0%. Positivity was detected in 31.9% (31/97) of samples. It was the first record of infection by Cryptosporidium spp. in dogs from northeastern Brazil. We verified significant association between the rate of infection and veterinary medical care, vaccination, and type of environment in which the dogs were kept.

Resumo O gênero Cryptosporidium é composto por protozoários com grande capacidade de reprodução e disseminação. Sua transmissão pode ocorrer indiretamente pela ingestão de água e alimentos contaminados com oocistos viáveis ou diretamente entre animais, entre humanos ou entre animais e humanos. Em diversas partes do mundo, animais de companhia tem sido citados como fontes potenciais de infecção por Cryptosporidium spp. O presente estudo teve como objetivo avaliar a ocorrência da infecção por Cryptosporidium spp. em cães domiciliados na cidade do Recife, estado de Pernambuco, Brasil, bem como a associação da infecção com dados epidemiológicos e sanitários. Foram analisadas 97 amostras de fezes de caninos domiciliados, utilizando-se as técnicas de centrífugo-sedimentação em formol-éter com posterior coloração pelo método de Kinyoun. A análise estatística foi realizada por meio dos testes Qui-quadrado de Pearson e Exato de Fisher, com nível de significância de 5,0%. Detectou-se positividade de 31,9% (31/97), tratando-se do primeiro registro de ocorrência da infecção por Cryptosporidium spp. na espécie canina no nordeste brasileiro, verificando-se associação significativa entre a taxa de infecção e a assistência médica veterinária, vacinação recebida e o tipo de ambiente de criação dos cães.