ABSTRACT
INTRODUCTION: The objective of this study was to evaluate the efficacy of passive ultrasonic irrigation (PUI) with 17% EDTA and 1% NaOCl solutions on smear layer removal. METHODS: Root canal preparations of 32 human teeth were performed with the ProTaper system. Next, they were longitudinally fractured to permit quantitation of smear layer creation from the cervical, middle, and apical thirds of the roots by using scanning electron microscopy. After reassembling the fractured tooth halves, they were divided into 4 groups according to different final irrigation protocols: group1, EDTA + NaOCl; group 2, EDTA with PUI + NaOCl; group 3, EDTA + NaOCl with PUI; and group 4, EDTA + NaOCl, both with PUI. After irrigation, the tooth halves were separated to permit imaging the same areas by scanning electron microscopy, and a percentage of opened dentinal tubules in irrigated areas as a percent of the total area was obtained. The results were submitted to Kruskal-Wallis, analysis of variance, and Bonferroni tests (α = 0.05). RESULTS: The cervical third of the samples from all groups showed higher percentage of smear layer removal and open dentinal tubule areas, followed by the middle and apical thirds. Among the irrigation groups, there were statistically significant differences in cervical third between group 2 and group 4 samples, with the highest and lowest percentage of smear layer removal, respectively. CONCLUSIONS: PUI by using 1% NaOCl and ultrasonic tip placed within 1 mm of the apical foramen did not show higher efficacy in smear layer removal compared with conventional irrigation.
Subject(s)
Root Canal Irrigants/therapeutic use , Root Canal Preparation/methods , Smear Layer/therapy , Ultrasonic Therapy/methods , Analysis of Variance , Edetic Acid/therapeutic use , Humans , Microscopy, Electron, Scanning , Random Allocation , Sodium Hypochlorite/therapeutic use , Tooth/drug effects , Tooth/ultrastructureABSTRACT
Milk has been studied extensively and has gained wide acceptance as a suitable storage medium capable of maintenance of avulsed teeth that cannot be replanted immediately. The objective of this study was to evaluate whether the renewal of milk as a storage medium every 24 h for up to 120 h is able to increase its ability to maintain human periodontal ligament fibroblasts (PDLF) viability in vitro. Plates with confluent PDLF were soaked in minimum essential medium (MEM) at 37°C (positive control) and in skimmed milk (22 wells) and water (negative control) for 24, 48, 72, 96, and 120 h at 5 and 20°C. The skimmed milk was renewed every 24 h in 11 of the wells of each plate. After these periods, cell viability was determined by the tetrazolium salt-based colorimetric (MTT) assay. Data were statistically analyzed by Kruskal-Wallis and Scheffé tests (α = 5%). At 24 h, milk and MEM performed similarly. However, from 48 h onwards, MEM was significantly better than renewed and not renewed milk at both temperatures. Regardless of temperature (5 or 20°C), renewal of milk with fresh milk did not affect its ability to maintain PDLF viability.
Subject(s)
Cell Survival , Culture Media/chemistry , Fibroblasts/chemistry , Milk/chemistry , Periodontal Ligament/chemistry , Tissue Preservation/methods , Animals , Cells, Cultured , Humans , Temperature , Tetrazolium Salts , Time FactorsABSTRACT
INTRODUCTION: The biological processes underlying the ability of mineral trioxide aggregate (MTA) to promote hard-tissue deposition and wound healing remain unclear. To further study these processes, specific signaling molecules related to the inflammatory response and the biomineralization process were analyzed to assess host-MTA interactions in vivo. METHODS: For cytokine level quantification and immunohistochemical analysis, human dentin tubes were filled with ProRoot MTA (Dentsply, Tulsa Dental, OK) or kept empty and were implanted in subcutaneous tissues in the backs of mice. Dentin tubes were retrieved and subsequently observed using a scanning electron microscope. RESULTS: MTA induced a time-dependent proinflammatory cytokine up-regulation up to 3 days. Immunohistochemical analyses showed an up-regulated expression of myeloperoxidase, nuclear factor-kappa B, activating protein-1, cyclooxygenase-2, inducible nitric oxide synthase, and vascular endothelial growth factor on day 1. Scanning electron microscopic examination revealed the presence of apatite-like clusters on collagen fibrils over the surface of tubes containing MTA. With the increase in time after implantation, a more extensive mineralization showing a compact layer of apatite was observed. CONCLUSION: MTA induced a proinflammatory and pro-wound healing environment. The biomineralization process occurred simultaneously at the biomaterial-dentin-tissue interface, with the acute inflammatory response. This promoted the integration of the biomaterial into the environment.
Subject(s)
Aluminum Compounds/pharmacology , Calcification, Physiologic/drug effects , Calcium Compounds/pharmacology , Inflammation Mediators/metabolism , Oxides/pharmacology , Root Canal Filling Materials/pharmacology , Signal Transduction/drug effects , Silicates/pharmacology , Animals , Apatites/analysis , Collagen/drug effects , Cyclooxygenase 2/drug effects , Dentin/ultrastructure , Drug Combinations , Humans , Interleukin-10/analysis , Interleukin-1beta/drug effects , Lymphocytes/pathology , Macrophages/pathology , Male , Mice , NF-kappa B/drug effects , Neutrophils/pathology , Nitric Oxide Synthase Type II/drug effects , Peroxidase/drug effects , Subcutaneous Tissue/drug effects , Time Factors , Transcription Factor AP-1/drug effects , Tumor Necrosis Factor-alpha/drug effects , Up-Regulation , Vascular Endothelial Growth Factor A/drug effectsABSTRACT
Neste artigo é relatado um caso de lesão cística periapical que se formou como resultado da necrose pulpar. Após o tratamento endodôntico, que inclui terapia a longo prazo com pasta de hidróxido de cálcio, ocorreu o reparo da lesão. A descrição deste caso clínico reforça a idéia de que lesões periapicais estensas, como cistos, podem responder favoravelmente ao tratamento endodontico convencional
Subject(s)
Humans , Female , Adult , Wounds and InjuriesABSTRACT
El propósito de la presente experiencia fue evaluar, en conductos mesiobucales de molares inferiores, la influencia que la rectificación de las paredes de la cámara pulpar y la preparación previa de los accesos tiene sobre la transportación apical. Se emplearon 20 molares inferiores humanos extraídos, los que fueron divididos al azar en 2 grupos de 10 molares cada uno. En el grupo A se rectificaron las paredes de la cámara pulpar y se prepararon los accesos al conducto radicular con fresas de Gates-Glidden #1 y #2. En el grupo B no se efectuó ninguna rectificación ni preparación a dicho nivel. En ambos grupos se instrumentaron los conductos mesiobucales con limas Flexofile del #15 al #35, en movimiento circunferencial. El grado de transportación apical fue evaluado radiográficamente empleando un proyector de perfiles. Los resultados mostraron menores valores de transportación en los conductos del grupo A, respecto de los del grupo B. En ambos grupos, la transportación apical fue mayor a medida que se incrmentó el calibre de los instrumentos (AU)
Subject(s)
Comparative Study , Humans , In Vitro Techniques , Tooth Root/diagnostic imaging , Root Canal Therapy/instrumentation , MolarABSTRACT
El propósito de la presente experiencia fue evaluar, en conductos mesiobucales de molares inferiores, la influencia que la rectificación de las paredes de la cámara pulpar y la preparación previa de los accesos tiene sobre la transportación apical. Se emplearon 20 molares inferiores humanos extraídos, los que fueron divididos al azar en 2 grupos de 10 molares cada uno. En el grupo A se rectificaron las paredes de la cámara pulpar y se prepararon los accesos al conducto radicular con fresas de Gates-Glidden #1 y #2. En el grupo B no se efectuó ninguna rectificación ni preparación a dicho nivel. En ambos grupos se instrumentaron los conductos mesiobucales con limas Flexofile del #15 al #35, en movimiento circunferencial. El grado de transportación apical fue evaluado radiográficamente empleando un proyector de perfiles. Los resultados mostraron menores valores de transportación en los conductos del grupo A, respecto de los del grupo B. En ambos grupos, la transportación apical fue mayor a medida que se incrmentó el calibre de los instrumentos