Identification of Spodoptera frugiperda importin alphas that facilitate the nuclear import of Autographa californica multiple nucleopolyhedrovirus DNA polymerase.

Proteins containing nuclear localization signals (NLSs) are actively transported into the nucleus via the classic importin-α/ß-mediated pathway, and NLSs are recognized by members of the importin-α family. Most studies of insect importin-αs have focused on Drosophila to date, little is known about the importin-α proteins in Lepidoptera insects. In this study, we identified four putative importin-α homologues, Spodoptera frugiperda importin-α1 (SfIMA1), SfIMA2, SfIMA4 and SfIMA7, from Sf9 cells. Immunofluorescence analysis showed that SfIMA2, SfIMA4 and SfIMA7 localized to the nucleus, while SfIMA1 distributed in cytoplasm. Additionally, SfIMA4 and SfIMA7 were also detected in the nuclear membrane of Sf9 cells. SfIMA1, SfIMA4 and SfIMA7, but not SfIMA2, were found to associate with the C terminus of AcMNPV DNA polymerase (DNApol) that harbours a typical monopartite NLS and a classic bipartite NLS. Further analysis of protein-protein interactions revealed that SfIMA1 specifically recognizes the bipartite NLS, while SfIMA4 and SfIMA7 bind to both monopartite and bipartite NLSs. Together, our results suggested that SfIMA1, SfIMA4 and SfIMA7 play important roles in the nuclear import of AcMNPV DNApol C terminus in Sf9 cells.

Insights into the molecular mechanism of the responses for Cyperus alternifolius to PhACs stress in constructed wetlands.

Cyperus alternifolius has been widely reported to be an effective phytoremediation plant in constructed wetland systems (CWs). In this context, an integrated biochemical and proteomic analysis of C. alternifolius leaves exposed to pharmaceutically active compounds (PhACs) in CWs was conducted to understand the mechanism of phytoremediation. The obtained results showed the antioxidant enzyme activities were induced throughout the experiment; however over time, the malondialdehyde content is not significantly different from the control and the photosynthetic pigment contents in plant were subsequently slowly recovered. Therefore, we concluded that reactive oxygen species could be effectively counteracted by the enhanced antioxidant enzyme activities, and therefore the photosynthetic pigments were ultimately restored. Leaf extract proteome maps were obtained through 2-DE, and an average of 55, 49, and 24 spots were significantly altered by 30, 100, and 500 µg/L of PhACs over the control, respectively. Protein expression patterns showed that proteins in C. alternifolius leaves are associated with photosynthesis, energy metabolism, defense, and protein synthesis. Moreover, the most relevant pathways modulated by PhACs were photosynthesis and energy metabolism. The protein expression involved in antioxidant defense and stress response generally increased in all the PhAC treatments. The regulated proteins may favor PhAC degradation in CWs; however, the role of these proteins in degrading PhACs remains unknown; further biochemical studies should be conducted. This study indicated that C. alternifolius can tolerate multiple PhACs.

Effects of improving nitrogen management on nitrogen utilization, nitrogen balance, and reactive nitrogen losses in a Mollisol with maize monoculture in Northeast China.

Traditional fertilization led to higher apparent N surplus, and optimized fertilization can reduce residual nitrogen in soils with keeping high yield. But in continuous spring maize cropping zone in Mollisol in Northeast China, the effect of the optimized N management on N balance and comprehensive environment was not clear. The primary objective of this study was to compare the differences of two fertilizations (traditional farmer N management (FNM) with single basal fertilizer and improvement N management (INM) by soil testing with top-dressing) in gain yield, N uptake and N efficiency, soil N balance, reactive N losses, and environment assessment. The results showed that INM treatment has no remarkable effect on grain yield and N uptake; N partial factor productivity (PFPN) of INM treatment was 19.8 % significantly higher than the FNM treatment. Nmin in soils of INM treatment reached to 111.0 kg ha(-1), which was 27.1 % lower than the FNM treatment after 6 years of continuous maize cropping; the apparent N Losses (ANL) and apparent N surplus (ANS) of INM were only half of FNM by soil N balance analysis. In reactive N losses, comparing with FNM treatment, INM treatment reduced NH3 volatilization, N2O emission, N leaching, and N runoff by 17.8, 35.6, 45, and 38.3 %, respectively, during planting period, and in integrated environment assessment by life cycle assessment (LCA) method, producing 1 t maize grain, energy depletion, acidification, eutrophication, and climate change impacts of INM treatment decreased 26.19, 30.16, 32.61, and 22.75 %, respectively. Therefore, INM treatment is a better N management strategy in comprehensive analysis.