A reflective display based on the electro-microfluidic assembly of particles within suppressed water-in-oil droplet array.

Reflective displays have stimulated considerable interest because of their friendly readability and low energy consumption. Herein, we develop a reflective display technique via an electro-microfluidic assembly of particles (eMAP) strategy whereby colored particles assemble into annular and planar structures inside a dyed water droplet to create "open" and "closed" states of a display pixel. Water-in-oil droplets are compressed within microwells to form a pixel array. The particles dispersed in droplets are driven by deformation-strengthened dielectrophoretic force to achieve fast and reversible motion and assemble into multiple structures. This eMAP based device can display designed information in three primary colors with ≥170° viewing angle, ~0.14 s switching time, and bistability with an optimized material system. This proposed technique demonstrates the basis of a high-performance and energy-saving reflective display, and the display speed and color quality could be further improved by structure and material optimization; exhibiting a potential reflective display technology.

Replaceable Dielectric Film for Low-Voltage and High-Performance Electrowetting-Based Digital Microfluidics.

Electrowetting-on-dielectric (EWOD) technology has been considered as a promising candidate for digital microfluidic (DMF) applications due to its outstanding flexibility and integrability. The dielectric layer with a hydrophobic surface is the key element of an EWOD device, determining its driving voltage, reliability, and lifetime. Hereby, inspired by the ionic-liquid-filled structuring polymer with high capacitance independent on thickness, namely ion gel (IG), we develop a polymer (P)-ion gel-amorphous fluoropolymer, namely, PIGAF, composite film as a replaceable hydrophobic dielectric layer for fabrication of a high-efficiency and stable EWOD-DMF device at relatively low voltage. The results show that the proposed EWOD devices using the PIGAF-based dielectric layer can achieve a large contact angle (θ) change of ∼50° and excellent reversibility with a contact angle hysteresis of ≤5° at a relatively low voltage of 30 Vrms. More importantly, the EWOD actuation voltage did not change obviously with the PIGAF film thickness in the range of several to tens of microns, enabling the thickness of the film to be adjusted according to the demand within a certain range while keeping the actuation voltage low. An EWOD-DMF device can be prepared by simply stacking a PIGAF film onto a PCB board, demonstrating stable droplet actuation (motion) at 30 Vrms and 1 kHz as well as a maximum moving velocity of 69 mm/s at 140 Vrms and 1 kHz. The PIGAF film was highly stable and reliable, maintaining excellent EWOD performance after multiple droplet manipulations (≥50 cycles) or long-term storage of 1 year. The proposed EWOD-DMF device has been demonstrated for digital chemical reactions and biomedical sensing applications.

Microwell Confined Electro-Coalescence for Rapid Formation of High-Throughput Droplet Array.

Droplet array is widely applied in single cell analysis, drug screening, protein crystallization, etc. This work proposes and validates a method for rapid formation of uniform droplet array based on microwell confined droplets electro-coalescence of screen-printed emulsion droplets, namely electro-coalescence droplet array (ECDA). The electro-coalescence of droplets is according to the polarization induced electrostatic and dielectrophoretic forces, and the dielectrowetting effect. The photolithographically fabricated microwells are highly regular and reproducible, ensuring identical volume and physical confinement to achieve uniform droplet array, and meanwhile the microwell isolation protects the paired water droplets from further fusion and broadens its feasibility to different fluidic systems. Under optimized conditions, a droplet array with an average diameter of 85 µm and a throughput of 106 in a 10 cm × 10 cm chip can be achieved within 5 s at 120 Vpp and 50 kHz. This ECDA chip is validated for various microwell geometries and functional materials. The optimized ECDA are successfully applied for digital viable bacteria counting, showing comparable results to the plate culture counting. Such an ECDA chip, as a digitizable and high-throughput platform, presents excellent potential for high-throughput screening, analysis, absolute quantification, etc.

Electro-Microfluidic Assembly Platform for Manipulating Colloidal Structures inside Water-in-Oil Emulsion Droplets.

Colloidal assembly is a key strategy in nature and artificial device. Hereby, an electromicrofluidic assembly platform (eMAP) is proposed and validated to achieve 3D colloidal assembly and manipulation within water droplets. The water-in-oil emulsion droplets autoposition in the eMAP driven by dielectrophoresis, where the (di)electrowetting effect induces droplet deformation, facilitating quadratic growth of the electric field in water droplet to achieve "far-field" dielectrophoretic colloidal assembly. Reconfigurable 3D colloidal configurations are observed and dynamically programmed via applied electric fields, colloidal properties, and droplet size. Binary and ternary colloidal assemblies in one droplet allow designable chemical and physical anisotropies for functional materials and devices. Integration of eMAP in high throughput enables mass production of functional microcapsules, and programmable optoelectronic units for display devices. This eMAP is a valuable reference for expanding fundamental and practical exploration of colloidal systems.

A liquid crystal-based biosensor for detection of insulin driven by conformational change of an aptamer at aqueous-liquid crystal interface.

Insulin is a critical predictor for the function of pancreatic islet beta cells, which plays a crucial role in diagnosing diabetes and diabetes-related disorders. Herein, we propose and validate a label-free and cost-effective aptamer-based optical LC biosensor for detection of insulin based on the directional recognition of biomolecular binding events at a responsive aqueous-liquid crystal (LC) interface. The binding of insulin and aptamer adsorbed on CTAB triggers a conformational change of the aptamer from G-quadruplex to stretched structure, inducing homeotropic to planar alignment and correspondingly dark to bright optical image change of the LC films. The molecular dynamic (MD) simulation validates that the orientational transition is associated with the interaction energy changes at the interface, which is in coordination with the optical observation. This LC biosensor takes advantages of simple preparation, easy operation, rapid sensing, high specificity for insulin determination in the range of 0.1-1.0 nM within 5 min. This sensor is also applicable for insulin detection in diluted human urine and serum. Additionally, the optical cell arrays allow to detection multiple samples of the same/different biomarkers at the same time. Such a strategy offers a potential basis for monitoring other clinical biomarkers, and for point-of-care testing (POCT) as well.

Design of Driving Waveform for Shortening Red Particles Response Time in Three-Color Electrophoretic Displays.

Three-color electrophoretic displays (EPDs) have the advantages of multi-color display and low power consumption. However, their red particles have the disadvantage of long response time. In this paper, a driving waveform, which is based on electrophoresis theory and reference gray scale optimization, was proposed to shorten the response time of red particles in three-color EPDs. The driving waveform was composed of erasing stage, reference gray scale forming stage, red driving stage, and white or black driving stage. Firstly, the characteristics of particle motion were analyzed by electrophoresis theory and Stokes law. Secondly, the reference gray scale of the driving waveform was optimized to shorten the distance between red particles and a common electrode plate. Finally, an experimental platform was developed to test the performance of the driving waveform. Experimental results showed that the proposed driving waveform can shorten the response time of red particles by 65.57% and reduce the number of flickers by 66.67% compared with the traditional driving waveform.

Design of Driving Waveform Based on a Damping Oscillation for Optimizing Red Saturation in Three-Color Electrophoretic Displays.

At present, three-color electrophoretic displays (EPDs) have problems of dim brightness and insufficient color saturation. In this paper, a driving waveform based on a damping oscillation was proposed to optimize the red saturation in three-color EPDs. The optimized driving waveform was composed of an erasing stage, a particles activation stage, a red electrophoretic particles purification stage, and a red display stage. The driving duration was set to 360 ms, 880 ms, 400 ms, and 2400 ms, respectively. The erasing stage was used to erase the current pixel state and refresh to a black state. The particles' activation stage was set as two cycles, and then refreshed to the black state. The red electrophoretic particles' purification stage was a damping oscillation driving waveform. The red and black electrophoretic particles were separated by changing the magnitude and polarity of applied electric filed, so that the red electrophoretic particles were purified. The red display stage was a low positive voltage, and red electrophoretic particles were driven to the common electrode to display a red state. The experimental results showed that the maximum red saturation could reach 0.583, which was increased by 27.57% compared with the traditional driving waveform.

Designing Splicing Digital Microfluidics Chips Based on Polytetrafluoroethylene Membrane.

As a laboratory-on-a-chip application tool, digital microfluidics (DMF) technology is widely used in DNA-based applications, clinical diagnosis, chemical synthesis, and other fields. Additional components (such as heaters, centrifuges, mixers, etc.) are required in practical applications on DMF devices. In this paper, a DMF chip interconnection method based on electrowetting-on-dielectric (EWOD) was proposed. An open modified slippery liquid-infused porous surface (SLIPS) membrane was used as the dielectric-hydrophobic layer material, which consisted of polytetrafluoroethylene (PTFE) membrane and silicone oil. Indium tin oxide (ITO) glass was used to manufacture the DMF chip. In order to test the relationship between the splicing gap and droplet moving, the effect of the different electrodes on/off time on the minimum driving voltage when the droplet crossed a splicing gap was investigated. Then, the effects of splicing gaps of different widths, splicing heights, and electrode misalignments were investigated, respectively. The experimental results showed that a driving voltage of 119 V was required for a droplet to cross a splicing gap width of 300 µm when the droplet volume was 10 µL and the electrode on/off time was 600 ms. At the same time, the droplet could climb a height difference of 150 µm with 145 V, and 141 V was required when the electrode misalignment was 1000 µm. Finally, the minimum voltage was not obviously changed, when the same volume droplet with different aqueous solutions crossed the splicing gap, and the droplet could cross different chip types. These splicing solutions show high potential for simultaneous detection of multiple components in human body fluids.

A novel p-n Mn0.2Cd0.8S/NiWO4 heterojunction for highly efficient photocatalytic H2 production.

Constructing a p-n heterojunction has been regarded as an effective way to restrain charge recombination and boost photocatalytic H2 production activity. Herein, a novel Mn0.2Cd0.8S/NiWO4 composite was fabricated by a hydrothermal process and which exhibited enhanced H2 production activity and excellent photostability. Particularly, the composite with 30 wt% of NiWO4 achieved the optimal H2 production rate of 17.76 mmol g-1 h-1, which was 2.9 times higher than that of Mn0.2Cd0.8S. The increased H2 production property was mainly due to the p-n heterojunction between Mn0.2Cd0.8S and NiWO4, which provided an efficient path for charge transfer and inhibited the photocorrosion of Mn0.2Cd0.8S. This work can offer technical support for the design and development of p-n heterojunctions that can be applied for photocatalytic H2 production.