Dataset of a flow intermittency study: Benthic communities of 13 alpine intermittent rivers.

In the last few decades, perennial mountain streams are becoming increasingly intermittent, due to global climate change and anthropogenic pressures. This phenomenon leads to negative effects on benthic communities' biodiversity and river ecosystems functionality. However, the impact of flow intermittency in previously perennial Alpine streams is still poorly investigated. This dataset consists of all the data collected during a spring sampling campaign performed in April-May 2017 along 13 mountain streams located in the SW Italian Alps. These watercourses have been selected because it was possible to identify two different sampling sites: one perennial, where water has always been flowing throughout the years, and one intermittent, which showed flowing water during the sampling campaign but, in the last decade, has experienced summer dry phases. All the sites have been characterized defining the microhabitats in which samples were retrieved, and physico-chemical data were collected at each site. Biological sampling included benthic macroinvertebrates and diatoms. Therefore, the present dataset offers various biological, ecological and physico-chemical information regarding Alpine streams which have recently become intermittent. Potentially, it could be used for comparisons with different benthic communities present in mountain rivers worldwide which are facing drying events too. The broad range of information present in this dataset offers the possibility to examine only the perennial sites themselves, as an example of good river functionality due to continuous flowing water, or only the intermittent ones, to better understand the effects of drying events on these peculiar ecosystems.

Dataset of a study about the impact of a micro-sewage effluent on the benthic macroinvertebrate community in a small Apennine creek (NW Italy).

Concerning the impact of organic contamination, most studies focus on the main river courses, which are affected by large wastewater plants and intensively urbanized areas, while a large part of a river's catchment area is made up of small streams flowing through rural or forested areas. As a result, the impact of even small sources of organic load on small systems is often not analysed. This study investigated the impact of a small sewage source on the aquatic environment of the Caramagna Creek (NW Italy). At the study site, the creek receives an effluent sewer from a small cluster of houses. To evaluate the impact of this point source of pollution, we estimated macroinvertebrate community composition and abundance monthly from January 2005 to March 2006 in two stations, located respectively 50 m upstream and 50 m downstream of the sewer pipe. At the same time, main physicochemical parameters, microbiological data, and chlorophyll-a concentration were assessed. These data aim to inspire additional research, particularly in addressing the implications of often overlooked small impacts occurring in small rivers, which can have an enormous impact given the dendritic organisation of the hydrographic network and the multiplicative effect along the river system. These results are especially relevant in the context of evolving river dynamics influenced by decreasing flows, resulting in a diminution in dilution capacity and thus greater fragility of river ecosystems. Moreover, if we consider only the upstream site, this dataset holds important potential related to non-impacted macroinvertebrate communities, constituting an important reference because it integrates macroinvertebrate community data with different environmental data, from granulometry to in-stream productivity, from chemical-physical to microbiological data.

Temporal effects of fine sediment deposition on benthic macroinvertebrate community structure, function and biodiversity likely reflects landscape setting.

Globally, excessive fine sediment (particles <2 mm) deposition is acknowledged to have deleterious effects on aquatic biodiversity. However, the impacts are often equivocal possibly reflecting landscape context, although this is rarely considered. To address this, we examined the temporal response of macroinvertebrate taxonomic and functional diversity to experimental fine sediment clogging in a prealpine (Italy) and lowland setting (UK). Colonisation devices were installed insitu with either clean or clogged substrates and examined for short (7-14 days), medium (21-28 days) and long (56-63 days) timescales. Clogging resulted in altered taxonomic community composition in both the lowland and prealpine rivers and modified functional community composition in the prealpine river. Nestedness was consistently found to be the dominant process driving differences in taxonomic composition between the clean and clogged substrates in the prealpine environment, with clogged substrates forming a nested community. No dominant component structured lowland taxonomic communities. Functional community composition was driven by nestedness in both environments but was heavily dominant in the case of the prealpine river, possibly reflecting low functional redundancy. Widely employed community richness metrics (EPT, taxa and functional richness) only displayed a response to fine sediment loading in the prealpine environment but taxa characterized as sensitive to fine sediment as well as some functional feeding groups did exhibit differences in both settings. In the prealpine environment, the effects of fine sediment intensified over time for several community metrics. Although further research is required to corroborate our findings and extend our observations across more rivers and typologies, excessive fine sediment is a pervasive stressor affecting macroinvertebrate communities in prealpine and lowland environments. However, the biodiversity facets that responded to clogging differed between the two landscape settings probably reflecting wider environmental filtering. Monitoring and managing fine sediment loading likely requires context specific approaches to maximise ecological benefits.

Biodiversity of leaf litter fungi in streams along a latitudinal gradient.

Global patterns of biodiversity have emerged for soil microorganisms, plants and animals, and the extraordinary significance of microbial functions in ecosystems is also well established. Virtually unknown, however, are large-scale patterns of microbial diversity in freshwaters, although these aquatic ecosystems are hotspots of biodiversity and biogeochemical processes. Here we report on the first large-scale study of biodiversity of leaf-litter fungi in streams along a latitudinal gradient unravelled by Illumina sequencing. The study is based on fungal communities colonizing standardized plant litter in 19 globally distributed stream locations between 69°N and 44°S. Fungal richness suggests a hump-shaped distribution along the latitudinal gradient. Strikingly, community composition of fungi was more clearly related to thermal preferences than to biogeography. Our results suggest that identifying differences in key environmental drivers, such as temperature, among taxa and ecosystem types is critical to unravel the global patterns of aquatic fungal diversity.

Effectiveness of artificial floods for benthic community recovery after sediment flushing from a dam.

The number of dams is predicted to increase worldwide under the current global change scenario. A major environmental problem associated with dams is the release of large quantities of fine sediment downstream. Therefore, future studies in river conservation will largely be focused on the management of sediments trapped by reservoirs. The aim of this study was to investigate the downstream ecological impacts of sediment flushing from a dam and the effectiveness of artificial flash floods as a recovery strategy. Artificial flash floods have often been employed to remove large amounts of sediment from riverbeds, but their importance in improving the biological quality of lotic environments is almost unknown. We carried out a series of quantitative macroinvertebrate samplings over a 2-year period that started before sediment release and included the artificial flushing events. We characterized the macroinvertebrate community in its structural and functional aspects and tested the performance of two biomonitoring indexes, comparing their diagnostic ability. Our results demonstrated that sediment flushing significantly altered the structure and composition of benthic communities for more than 1 year. Flash floods exacerbated the overall biological quality, but we believe that this treatment was useful because, by removing large amounts of sediment, the biological recovery process was accelerated. Finally, regarding the water quality assessment, we found that the biomonitoring index for siltation, composed of a selection of taxonomical and functional metrics, was more reliable than the generic one.

Trophic availability buffers the detrimental effects of clogging in an alpine stream.

Clogging, the streambed colmation by fine sediments, is an important widespread source of impact affecting freshwaters. Alterations in stream morphology and hydrology, added to the effects of global climate change, are responsible for this phenomenon, that is particularly pernicious in mountainous lotic systems naturally characterized by coarse substrates. Among the studies investigating this issue some were descriptive, while others used artificial substrates to compare ongoing fine sediment accumulation and macroinvertebrate assemblage recruitment. Other studies used from the outset artificial substrates arranged with different levels of clogging. Our study fits into this line, but adding an innovative element simulating different availability of coarse particulate organic matter, i.e. the main trophic input in low-order, mountainous stream. To investigate how clogging and CPOM can influence macroinvertebrate communities, we placed 135 artificial substrates in the upper Po river (NW Italy). We set up a three way factorial design with three different levels of sedimentation and terrestrial leaf material. Artificial substrates were removed on three different dates. Benthic invertebrates were identified and classified according to their bio-ecological traits. We also measured macroinvertebrate dry mass and CPOM degradation in the different trap types. Our findings show that clogging acts as a selective filter influencing taxa richness, density, functional composition and biomass of benthic assemblage. Moreover, fine sediments affect the energetic dynamics in the river ecosystem, decreasing the mass loss rate of terrestrial leaves. Interestingly, our results clearly demonstrate that high availability of CPOM can buffer the negative effect of clogging, suggesting that an adequate input of allochthonous organic matter may lessen the impact of fine sediment deposition. Because land use transformation and removal of wooded riparian areas increase clogging and simultaneously reduces the input of CPOM, our findings stress the importance to include the management of river basins in the conservation strategies of mountainous streams.

Freshwater ecosystems and aquatic insects: a paradox in biological invasions.

Biological invasions have increased significantly in response to global change and constitute one of the major causes of biodiversity loss. Insects make up a large fraction of invasive species, in general, and freshwaters are among the most invaded ecosystems on our planet. However, even though aquatic insects dominate most inland waters, have unparalleled taxonomic diversity and occupy nearly all trophic niches, there are almost no invasive insects in freshwaters. We present some hypotheses regarding why aquatic insects are not common among aquatic invasive organisms, suggesting that it may be the result of a suite of biological, ecological and anthropogenic factors. Such specific knowledge introduces a paradox in the current scientific discussion on invasive species; therefore, a more in-depth understanding could be an invaluable aid to disentangling how and why biological invasions occur.

Development and application of an innovative expert decision support system to manage sediments and to assess environmental risk in freshwater ecosystems.

With the aim of supporting decision makers to manage contamination in freshwater environments, an innovative expert decision support system (EDSS) was developed. The EDSS was applied in a sediment quality assessment along the Bormida river (NW, Italy) which has been heavily contaminated by an upstream industrial site for more than a century. Sampling sites were classified by means of comparing chemical concentrations with effect-based target values (threshold and probable effect concentrations). The level of each contaminant and the combined toxic pressure were used to rank sites into three categories: (i) uncontaminated (8 sites), (ii) mildly contaminated (4) and (iii) heavily contaminated (19). In heavily contaminated sediments, an environmental risk index (EnvRI) was determined by means of integrating chemical data with ecotoxicological and ecological parameters (triad approach). In addition a sediment risk index (SedRI) was computed from combining chemical and ecotoxicological data. Eight sites exhibited EnvRI values ≥0.25, the safety threshold level (range of EnvRI values: 0.14-0.31) whereas SedRI exceeded the safety threshold level at 6 sites (range of SedRI values: 0.16-0.36). At sites classified as mildly contaminated, sublethal biomarkers were integrated with chemical data into a biological vulnerability index (BVI), which exceeded the safety threshold level at one site (BVI value: 0.28). Finally, potential human risk was assessed in selected stations (11 sites) by integrating genotoxicity biomarkers (GTI index falling in the range 0.00-0.53). General conclusions drawn from the EDSS data include: (i) in sites classified as heavily contaminated, only a few exhibited some significant, yet limited, effects on biodiversity; (ii) restrictions in re-using sediments from heavily contaminated sites found little support in ecotoxicological data; (iii) in the majority of the sites classified as mildly contaminated, tested organisms exhibited low response levels; (iv) preliminary results on genotoxicity biomarkers indicate possible negative consequences for humans if exposed to river sediments from target areas.

Feeding habits, fine structure and microhabitat preference of Euthyplocia hecuba (Hagen, 1861) (Ephemeroptera: Euthyplociidae) nymphs from Honduras.

The Euthyplociidae are a tropical and subtropical Ephemeropteran group. The aim of this study was to augment knowledge on some aspects of the biology and ecology of Euthyplocia hecuba (Hagen, 1861) nymphs. Mayflies were collected in the Rio El Padre, located on the Caribbean slope of Honduras. Diet, microhabitat preference and some fine morphological aspects of the nymphs were examined. E. hecuba nymphs are very specialised organisms that occupy a specific ecological niche in the lotic/benthic community. Immature stages show an evident preference for life in soft and fine substrata. They are detritivorous burrowers that ingest large amounts of fine particles deposited by river flow in sedimentation areas. They show peculiar morphological adaptations for life in this particular substratum, such as mouthparts modified for digging and collection of fine organic sediments, digging legs with shovel-like tibiae and massive femora, and filamentous gills.

A "weight of evidence" approach for the integration of environmental "triad" data to assess ecological risk and biological vulnerability.

A new Expert Decision Support System (EDSS) that can integrate Triad data for assessing environmental risk and biological vulnerability at contaminated sites has been developed. Starting with ecosystem relevance, the EDSS assigns different weights to the results obtained from Triad disciplines. The following parameters have been employed: 1) chemical soil analyses (revealing the presence of potentially dangerous substances), 2) ecotoxicological bioassays (utilizing classical endpoints such as survival and reproduction rates), 3) biomarkers (showing sublethal pollutant effects), and 4) ecological parameters (assessing changes in community structure and functions). For each Triad discipline, the EDSS compares the data obtained at the studied field sites with reference values and calculates different 0-1 indexes (e.g., Chemical Risk Index, Ecotoxicological Risk Index, and Ecological Risk Index). The EDSS output consists of 3 indexes: 1) Environmental Risk index (EnvRI), quantifying the levels of biological damage at population-community level, 2) Biological Vulnerability Index (BVI), assessing the potential threats to biological equilibriums, and 3) Genotoxicity Index (GTI), screening genotoxicity effects. The EDSS has been applied in the integration of a battery of Triad data obtained during the European Union-funded Life Intervention in the Fraschetta Area (LINFA) project, which has been carried out in order to estimate the potential risk from soils of a highly anthropized area (Alessandria, Italy) mainly impacted by deposition of atmospheric pollutants. Results obtained during 4 seasonal sampling campaigns (2004-2005) show maximum values of EnvRI in sites A and B (characterized by industrial releases) and lower levels in site D (affected by vehicular traffic emissions). All 3 potentially polluted sites have shown high levels of BVI and GTI, suggesting a general change from reference conditions (site C).

Use of highly sensitive sublethal stress responses in the social amoeba Dictyostelium discoideum for an assessment of freshwater quality.

In this work, the sensitivity of a battery of tests on the social amoeba Dictyostelium discoideum has been assessed within a freshwater toxicity study. The results obtained from the evaluation of survival and replication rate of D. discoideum were compared to those derived with a series of widely used tests for freshwater toxicity assessment, i. e. bioassays using Vibrio fischeri, Daphnia magna and Pseudokirchneriella subcapitata. The effects on sublethal endpoints, i.e. lysosomal membrane stability (LMS) and endocytotic rate, were analysed in conjunction with high-level endpoints to verify the potential to make a typical bioassay more sensitive. The field ecotoxicological investigation employing D. discoideum is part of a monitoring study assessing environmental quality of the Bormida River (Italy), subjected until recently to a chronic industrial pollution. The survey was carried out at several stations (upstream and downstream of a chemical factory outlet) in two different periods. In 2002, the results of chemical analyses performed on river water indicated no contamination. The ecotoxicological data obtained in this period showed that no evidence of biological effects was observed using V. fischeri and D. magna bioassays. In spite of the previous classical acute toxicity tests, significant differences in cell viability of D. discoideum were found. By analysing the effects measured on LMS and endocytotic rate, more relevant changes were observed for these sublethal stress biomarkers compared to survival. The chronic toxicity data showed significant changes in cell growth both of P. subcapitata and D. discoideum. Nevertheless, more sensitive and rapid responses were obtained when assessing the effects of exposure on D. discoideum. The chemical and ecotoxicological data obtained in 2006 indicated a full recovery of the quality of the river water (neither contamination nor toxicity found). Altogether, the results reported in this study underline that the use of a battery of biomarkers in conjunction with high-level endpoints may help follow the pollutant-induced stress syndrome in the organisms from early sublethal effects to starting mortality.

A characterization of the egg capsules of Anacroneuria starki and A. talamanca (Plecoptera: Perlidae), with a suggestion about the distribution of stoneflies in the Tropics.

In this study, the structure of egg capsules of two species of Neotropical Perlidae: Anacroneuria starki Fenoglio and Morisi (2001 a) and A. talamanca Stark (1998), were examined. Eggs were studied using a scanning electron microscope. A morphological characterization of these eggshells is presented: layers, attachment structures and other anatomical features are described. The egg capsules of the two species differ in some aspects, but both are characterized by thin-layered egg coverings (vitelline envelope, chorion and extrachorion). On the basis of these observations, the importance of eggshell structure for the biogeographical distribution of Plecoptera in the tropics is discussed. A key role for egg capsules in the adaptation process of Plecoptera to aquatic environments of the Neotropics is hypothesized.

Benthic macroinvertebrate communities as indicators of river environment quality: an experience in Nicaragua

Biotic indexes are one of several types of measures that are routinely used in biological monitoring in temperate streams and offer interesting possibilities to assess the environmental quality of rivers in the neotropics. Macroinvertebrate communities of seven southeastern Nicaraguan streams were monitored and seven ecological indexes were applied. The results suggest that information from the Indice Biótico Esteso (I.B.E.) is closely correlated to the results of other methods, but the I.B.E. index is easier to apply as well as avoiding high costs and time-consuming procedures. A calibration of the method is necessary for rapid assessment approaches in the neotropics

Benthic macroinvertebrate communities as indicators of river environment quality: an experience in Nicaragua.

Biotic indexes are one of several types of measures that are routinely used in biological monitoring in temperate streams and offer interesting possibilities to assess the environmental quality of rivers in the neotropics. Macroinvertebrate communities of seven southeastern Nicaraguan streams were monitored and seven ecological indexes were applied. The results suggest that information from the Indice Biótico Esteso (I.B.E.) is closely correlated to the results of other methods, but the I.B.E. index is easier to apply as well as avoiding high costs and time-consuming procedures. A calibration of the method is necessary for rapid assessment approaches in the neotropics.