The Influence of Environmental Variation on the Genetic Structure of a Poison Frog Distributed Across Continuous Amazonian Rainforest.

Biogeographic barriers such as rivers have been shown to shape spatial patterns of biodiversity in the Amazon basin, yet relatively little is known about the distribution of genetic variation across continuous rainforest. Here, we characterize the genetic structure of the brilliant-thighed poison frog (Allobates femoralis) across an 880-km-long transect along the Purus-Madeira interfluve south of the Amazon river, based on 64 individuals genotyped at 7609 single-nucleotide polymorphism (SNP) loci. A population tree and clustering analyses revealed 4 distinct genetic groups, one of which was strongly divergent. These genetic groups were concomitant with femoral spot coloration differences, which was intermediate within a zone of admixture between two of the groups. The location of these genetic groups did not consistently correspond to current ecological transitions between major forest types. A multimodel approach to quantify the relative influence of isolation-by-geographic distance (IBD) and isolation-by-environmental resistance (IBR) nevertheless revealed that, in addition to a strong signal of IBD, spatial genetic differentiation was explained by IBR primarily linked to dry season intensity (r2 = 8.4%) and canopy cover (r2 = 6.4%). We show significant phylogenetic divergence in the absence of obvious biogeographical barriers and that finer-scaled measures of genetic structure are associated with environmental variables also known to predict the density of A. femoralis.

Soil and forest structure predicts large-scale patterns of occurrence and local abundance of a widespread Amazonian frog.

The distribution of biodiversity within the Amazon basin is often structured by sharp environmental boundaries, such as large rivers. The Amazon region is also characterized by subtle environmental clines, but how they might affect the distributions and abundance of organisms has so far received less attention. Here, we test whether soil and forest characteristics are associated with the occurrence and relative abundance of the forest-floor dwelling Aromobatid frog, Allobates femoralis. We applied a structured sampling regime along an 880 km long transect through forest of different density. High detection probabilities were estimated for A. femoralis in each of the sampling modules. Using generalized linear mixed-effects models and simple linear regressions that take detectability into account, we show that A. femoralis is more abundant in open forests than in dense forests. The presence and relative abundance of A. femoralis is also positively associated with clay-rich soils, which are poorly drained and therefore likely support the standing water bodies required for reproduction. Taken together, we demonstrate that relatively easy-to-measure environmental features can explain the distribution and abundance of a widespread species at different spatial scales. Such proxies are of clear value to ecologists and conservation managers working in large inaccessible areas such as the Amazon basin.

Changes in leaf chlorophyll content associated with flowering and its role in the diversity of phytophagous insects in a tree species from a semiarid Caatinga.

Phytophagous insects choose their feeding resources according to their own requirements, but their feeding preferences in the semiarid Caatinga have rarely been studied. Flowering trees leads to a greater diversity of flower visitors and their predators in the host plant, but little is known about why the diversity of phytophagous insects not associated with flowers is also increased. The purpose of this study was to evaluate the diversity of sap-sucking, wood-boring and leaf-chewing insects associated with leaf chlorophyll content in flowering and non-flowering plants of Poincianella pyramidalis, an endemic tree of Caatinga. We used a leaf chlorophyll index (LCI) as a surrogate for resource quality, and an entomological umbrella to collect phytophagous insects. We show that trees which bloomed demonstrated higher chlorophyll content, greater abundance and a significant difference in the composition of phytophagous insect species when compared to non-flowering trees (p < 0.05). The results suggest that not only the presence of flowers themselves, but also the higher nutritional quality of leaf tissue, can explain the differences in species diversity and abundance of phytophagous insects. Exceptional flowering trees in the Caatinga area studied may thus act as spots of high quality resources, favouring changes in the diversity of insects in this environment.